Journal: Journal of Clinical Microbiology
Article Title: Rapid and Specific Detection of tdh, trh1, and trh2 mRNA of Vibrio parahaemolyticus by Transcription-Reverse Transcription Concerted Reaction with an Automated System
Figure Lengend Snippet: Real-time monitoring of TRC reactions for RNA preparations of control strains of V. parahaemolyticus by using TRC assays to detect tdh , trh 1, and trh 2 mRNAs. The profiles of the TRC reactions for detection of the tdh mRNA (A), the trh 1 mRNA (B), and the trh 2 mRNA (C) are shown. The total RNA extracted from AQ3815 (red dots; tdh 1 positive, tdh 2 positive, trh 1 negative, and trh 2 negative), AQ3776 (blue dots; tdh 3 positive, tdh 4 positive, trh 1 positive, and trh 2 negative), AQ3860 (green dots; tdh 5 positive, trh 1 positive, and trh 2 negative), AQ4037 (pink dots; tdh negative, trh 1 positive, and trh 2 negative), AT4 (pale-blue dots; tdh negative, trh 1 negative, and trh 2 positive), and DOH272 (gray dots; tdh negative, trh 1 negative, and trh 2 negative) were examined.
Article Snippet: The high-level tdh transcription in AQ3776 may be due to the copy number effect of the tdh 4 gene.