Journal: Cancer Science

Article Title: NKG2D defines tumor‐reacting effector CD8 + T cells within tumor microenvironment

doi: 10.1111/cas.15050

Figure Lengend Snippet: Expression of transcription factors in tumor antigen‐specific CD8 + T cells in the immune control effector phase. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. B16OVA‐Luc2 cells (10 5 cells/mouse) were used to sc inoculate vaccinated C57BL/6 mice. At the indicated time‐points, TIL were collected and subjected to flow cytometry (Day 0 = tumor inoculation). Representative histograms (left) and summaries (right) of Eomes (A) and T‐bet (B) expression in OVATet + T cells are shown. C, Representative contour plots (left) and summaries (middle) of TOX expression (TOX hi ) on OVATet + T cells in TIL are shown. The percentage of TOX hi OVATet + T cells isolated from the indicated sources is shown (right). Data are presented as the mean ± SEM

Article Snippet: For intracellular staining, cells were fixed and permeabilized in accordance with the manufacturer's instructions (FoxP3/Transcription factor staining buffer set, eBioscence) before being stained with fluorophore‐labeled antibodies: anti‐mouse eomesodermin (Eomes, clone REA116, MACS Miltenyi Biotec), T‐bet (4B10, BioLegend), and TOX (REA473, Miltenyi Biotec).

Techniques: Expressing, Control, Flow Cytometry, Isolation

Journal: bioRxiv

Article Title: Assembly of neuron- and radial glial cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons

doi: 10.1101/2022.09.03.506497

Figure Lengend Snippet: Histochemical analysis of radial glial cells, intermediate progenitor cells, and the morphology of radial fibers. ( A ) Immunostaining of Pax6-positive radial glial cells and Tbr2-positive intermediate progenitor cells in the VZ of WT and DKO cerebral cortices at E16. Bar graphs show the numbers of Pax6- and Tbr2-positive cells per 150 × 150 μm 2 . N = 5 mice per group. Mean ± SD; ns > 0.05; the Student’s t -test. ( B ) Immunostaining of nestin-positive radial fibers in WT and DKO cerebral cortices at E16. ( C ) High magnification images of mCherry-positive bipolar neurons and radial fibers in the WT and DKO at E16, 2 days after in utero labeling. Bipolar neurons in the SP/IZ-attached radial fiber, irrespective of genotypes. Scale bars represent 20 μm (A), 50 μm (B), and 5 μm (C).

Article Snippet: Sections were incubated at 4°C overnight with biotinylated HA-binding protein (b-HABP) (1:400, Hokudo), anti-NCAN (sheep, 1:400, R&D), anti-TNC (rat, 1:400, R&D), anti-Pax6 (rabbit, 1:400, Invitrogen), anti-Tbr2 (mouse, 1:400, Wako), anti-Nestin (mouse, 1:400, Millipore), and Alexa Fluor 488-conjugated anti GFP (rabbit, 1:1000, Invitrogen).

Techniques: Immunostaining, In Utero, Labeling