tbe buffer Search Results


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  • 99
    Thermo Fisher tbe tris borate edta buffer
    Characterization of barcoded 601 (BC-601) DNA a , BC-601 DNA prepared for all 115 nucleosome library members as described in Methods (Barcoded 601 (BC-601) DNA preparation). Ligation products are 192 bp in size and were visualized by polyacrylamide gel electrophoresis (5% acrylamide, <t>0.5×</t> <t>TBE,</t> 200 V, 40 min) and staining with SYBR Safe DNA gel stain. A faint band corresponding to unligated 601 DNA (601) is slightly visible in certain cases. b .
    Tbe Tris Borate Edta Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1706 article reviews
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    tbe tris borate edta buffer - by Bioz Stars, 2020-04
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    99
    Millipore tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 520 article reviews
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    tbe buffer - by Bioz Stars, 2020-04
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    96
    TaKaRa tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 1x tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    1x Tbe Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1x tbe buffer - by Bioz Stars, 2020-04
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    99
    TaKaRa tris borate edta tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Tbe Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Fisher Scientific tris borate edta buffer tbe
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Buffer Tbe, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher ethylenediaminetetraacetic acid tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Ethylenediaminetetraacetic Acid Tbe Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 3 article reviews
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    99
    Millipore tbe buffer concentrate
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Buffer Concentrate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 6 article reviews
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    tbe buffer concentrate - by Bioz Stars, 2020-04
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    96
    Amresco tris borate edta tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Tbe Buffer, supplied by Amresco, used in various techniques. Bioz Stars score: 96/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher tbe urea sample buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Urea Sample Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 47 article reviews
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    tbe urea sample buffer - by Bioz Stars, 2020-04
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    97
    Bio-Rad tris borate edta tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Tbe Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 272 article reviews
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    95
    Promega tris borate edta tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Tbe Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Bio-Rad tris borate edta buffer tbe page
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Buffer Tbe Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher tbe urea loading buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Urea Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 33 article reviews
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    tbe urea loading buffer - by Bioz Stars, 2020-04
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    85
    Mediatech tris borate edta tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Tbe Buffer, supplied by Mediatech, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 19 article reviews
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    91
    GE Healthcare tbe tris borate edta buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Tris Borate Edta Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 17 article reviews
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    91
    Biotium tris borate edta buffer tbe
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Buffer Tbe, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Roche tbe tris borate edta buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Tris Borate Edta Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 81/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    CinnaGen Co tbe tris borate edta buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tbe Tris Borate Edta Buffer, supplied by CinnaGen Co, used in various techniques. Bioz Stars score: 97/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck & Co tris borate edta buffer tbe
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Buffer Tbe, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Melford Laboratories 1x tris borate edta tbe buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    1x Tris Borate Edta Tbe Buffer, supplied by Melford Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Nippon Genetics tris borate edta buffer tbe
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Buffer Tbe, supplied by Nippon Genetics, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tris borate edta buffer tbe - by Bioz Stars, 2020-04
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    87
    Amresco tris borate edta buffer
    <t>CovR-D53A</t> and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% <t>TBE</t> polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Tris Borate Edta Buffer, supplied by Amresco, used in various techniques. Bioz Stars score: 87/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× <t>Tris-borate-EDTA</t> buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
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    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× <t>Tris-borate-EDTA</t> buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
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    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× <t>Tris-borate-EDTA</t> buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
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    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× <t>Tris-borate-EDTA</t> buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
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    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× <t>Tris-borate-EDTA</t> buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
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    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× <t>Tris-borate-EDTA</t> buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
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    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× <t>Tris-borate-EDTA</t> buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
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    Image Search Results


    Characterization of barcoded 601 (BC-601) DNA a , BC-601 DNA prepared for all 115 nucleosome library members as described in Methods (Barcoded 601 (BC-601) DNA preparation). Ligation products are 192 bp in size and were visualized by polyacrylamide gel electrophoresis (5% acrylamide, 0.5× TBE, 200 V, 40 min) and staining with SYBR Safe DNA gel stain. A faint band corresponding to unligated 601 DNA (601) is slightly visible in certain cases. b .

    Journal: Nature

    Article Title: ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference

    doi: 10.1038/nature23671

    Figure Lengend Snippet: Characterization of barcoded 601 (BC-601) DNA a , BC-601 DNA prepared for all 115 nucleosome library members as described in Methods (Barcoded 601 (BC-601) DNA preparation). Ligation products are 192 bp in size and were visualized by polyacrylamide gel electrophoresis (5% acrylamide, 0.5× TBE, 200 V, 40 min) and staining with SYBR Safe DNA gel stain. A faint band corresponding to unligated 601 DNA (601) is slightly visible in certain cases. b .

    Article Snippet: Quenched assay samples were directly run on a 5% TBE gel in 0.5× TBE buffer for 40 min at 200 V. Nucleosomes were visualized by staining with SYBR Gold Nucleic Acid Gel Stain (ThermoFisher Scientific).

    Techniques: Ligation, Polyacrylamide Gel Electrophoresis, Staining

    DsrA DII competes with full-length DsrA for binding to Hfq. 5′-end-labeled DsrA was incubated with increasing concentrations of DsrA DI , DsrA DII , and DsrA DIII at 25°C for 5 min, followed by the addition of Hfq and an incubation at 25°C for 5 min. Samples were separated on an 8% native polyacrylamide gel in 1× TBE. ( A ) DsrA DI , ( B ) DsrA DII , and ( C ) DsrA DIII were incubated with full-length 5′-end-labeled DsrA. Samples were incubated without (−) or with (+) 3 μM Hfq. ( D ) 5′-end-labeled full-length DsrA (+), DsrA DI (•), DsrA DII (▴), or DsrA DIII (▪) were incubated at 25°C for 10 min with the indicated amounts of purified Hfq. The percent of unbound RNA was determined using a phosphorimager (see Materials and Methods) and plotted against Hfq concentration. Only full-length DsrA and DsrA DII bound to Hfq.

    Journal: RNA

    Article Title: Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure

    doi: 10.1261/rna.2570803

    Figure Lengend Snippet: DsrA DII competes with full-length DsrA for binding to Hfq. 5′-end-labeled DsrA was incubated with increasing concentrations of DsrA DI , DsrA DII , and DsrA DIII at 25°C for 5 min, followed by the addition of Hfq and an incubation at 25°C for 5 min. Samples were separated on an 8% native polyacrylamide gel in 1× TBE. ( A ) DsrA DI , ( B ) DsrA DII , and ( C ) DsrA DIII were incubated with full-length 5′-end-labeled DsrA. Samples were incubated without (−) or with (+) 3 μM Hfq. ( D ) 5′-end-labeled full-length DsrA (+), DsrA DI (•), DsrA DII (▴), or DsrA DIII (▪) were incubated at 25°C for 10 min with the indicated amounts of purified Hfq. The percent of unbound RNA was determined using a phosphorimager (see Materials and Methods) and plotted against Hfq concentration. Only full-length DsrA and DsrA DII bound to Hfq.

    Article Snippet: Samples were then mixed with 2 μL of Hi-Density TBE sample buffer (Invitrogen), analyzed on an 8% native polyacrylamide gel in 1× TBE (Invitrogen) at 100 V for 2 h, dried, and exposed to a PhosphorImager screen (Molecular Dynamics).

    Techniques: Binding Assay, Labeling, Incubation, Purification, Concentration Assay

    Quantitative AGE assay development. ( A ) Ethidium bromide versus Sybr-Gold ‘Staining’. Aliquots of 200 ng of four plasmid batches known to contain differing levels of recombinant plasmid were separated by AGE and then stained with 1× TBE containing either ethidium bromide (50 ng/ml) (left gel) or 1× Sybr-Gold (right gel) for 30 min prior to gel-image capture by Polaroid camera. Arrows indicate the position of low-level recombinants only clearly visible with Sybr-Gold staining. ( B ) Image analysis of a 1× Sybr-Gold stained gel using manufacture's recommended staining times. Image capture by ProXpress. For this analysis, 1, 2 and 5 μl volumes of MassRuler High Range DNA Ladder (MBI-Fermentas, Lithuania) were loaded in lanes 1, 2 and 3, respectively. Left panel, gel image with loads corresponding to DNA quantities (per band) ranging from 1.6 to 10 ng (lane 1), 3.2 to 20 ng (lane 2) and 8 to 50 ng (lane 3). Middle panel, schematic ImageQuant (Molecular Dynamics, SunnyVale, CA) generated electropherogram traces of each lane. Right panel, scatter-plot with trend-line representation of area-under-curve signal ( y -axis) against quantity (in nanograms) of DNA ( x -axis) of the combined 1.6 ng through to 50 ng results. This plot highlights the significant signal ‘plateau’ that develops above 50 ng loads ( C ) Extending assay linear range by increasing Sybr-Gold staining times. Triplicate 5 μl (lanes 1, 3 and 5) and 30 μl (lanes 2, 4 and 6) MassRuler DNA ladder loads were separated by AGE. Subsequently, the gel was then dissected longitudinally into three parts and stained for 20 min (lanes 1 and 2), 2 h (lanes 3 and 4) or 24 h (lanes 5 and 6). After such staining regimes, excess stain was removed by copious washing with water. Image analysis by ProXpress and scatter plot data representation was then undertaken as described above in (B). The 5 and 30 μl loads generate a collective DNA species quantitative range of between 8 and 300 ng. The results of analysis reveal the R 2 values obtained for the 20 min, 2 h and 24 h staining regimes are 0.910, 0.990 and 0.999, respectively.

    Journal: Nucleic Acids Research

    Article Title: Effective and robust plasmid topology analysis and the subsequent characterization of the plasmid isoforms thereby observed

    doi: 10.1093/nar/gnh124

    Figure Lengend Snippet: Quantitative AGE assay development. ( A ) Ethidium bromide versus Sybr-Gold ‘Staining’. Aliquots of 200 ng of four plasmid batches known to contain differing levels of recombinant plasmid were separated by AGE and then stained with 1× TBE containing either ethidium bromide (50 ng/ml) (left gel) or 1× Sybr-Gold (right gel) for 30 min prior to gel-image capture by Polaroid camera. Arrows indicate the position of low-level recombinants only clearly visible with Sybr-Gold staining. ( B ) Image analysis of a 1× Sybr-Gold stained gel using manufacture's recommended staining times. Image capture by ProXpress. For this analysis, 1, 2 and 5 μl volumes of MassRuler High Range DNA Ladder (MBI-Fermentas, Lithuania) were loaded in lanes 1, 2 and 3, respectively. Left panel, gel image with loads corresponding to DNA quantities (per band) ranging from 1.6 to 10 ng (lane 1), 3.2 to 20 ng (lane 2) and 8 to 50 ng (lane 3). Middle panel, schematic ImageQuant (Molecular Dynamics, SunnyVale, CA) generated electropherogram traces of each lane. Right panel, scatter-plot with trend-line representation of area-under-curve signal ( y -axis) against quantity (in nanograms) of DNA ( x -axis) of the combined 1.6 ng through to 50 ng results. This plot highlights the significant signal ‘plateau’ that develops above 50 ng loads ( C ) Extending assay linear range by increasing Sybr-Gold staining times. Triplicate 5 μl (lanes 1, 3 and 5) and 30 μl (lanes 2, 4 and 6) MassRuler DNA ladder loads were separated by AGE. Subsequently, the gel was then dissected longitudinally into three parts and stained for 20 min (lanes 1 and 2), 2 h (lanes 3 and 4) or 24 h (lanes 5 and 6). After such staining regimes, excess stain was removed by copious washing with water. Image analysis by ProXpress and scatter plot data representation was then undertaken as described above in (B). The 5 and 30 μl loads generate a collective DNA species quantitative range of between 8 and 300 ng. The results of analysis reveal the R 2 values obtained for the 20 min, 2 h and 24 h staining regimes are 0.910, 0.990 and 0.999, respectively.

    Article Snippet: The 1× TBE running buffer also contained 5.0 μg/ml chloroquine, and electrophoresis was performed at 50 V for 48 h. Staining was carried out by soaking in 1× TBE containing 1× Sybr-Gold (Molecular Probes) for 24 h in the dark.

    Techniques: Staining, Plasmid Preparation, Recombinant, Generated

    CovR-D53A and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% TBE polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.

    Journal: PLoS Pathogens

    Article Title: Dual-Site Phosphorylation of the Control of Virulence Regulator Impacts Group A Streptococcal Global Gene Expression and Pathogenesis

    doi: 10.1371/journal.ppat.1004088

    Figure Lengend Snippet: CovR-D53A and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% TBE polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.

    Article Snippet: The purified PCR products (0.4 µg) were incubated with indicated amounts of various CovR isoforms at 37°C for 15 min in TBE-buffer (89 mM Tris, 89 mM borate, 1 mM EDTA, 5% glycerol, and 10 µg/ml polydI:dC (Sigma)) as described .

    Techniques: Binding Assay, Recombinant, Concentration Assay, Incubation, Staining, Molecular Weight

    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Journal: Applied and Environmental Microbiology

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia

    doi:

    Figure Lengend Snippet: PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Article Snippet: It was then incubated at 4°C for 16 h and, finally, digestion was performed at 37°C for another 48 h. High-molecular-weight restriction fragments were resolved in 1% agarose gel in 0.5% Tris-borate-EDTA buffer by using a CHEF apparatus (CHEF-DR II; Bio-Rad Laboratories, Richmond, Calif.).

    Techniques: Isolation, Agarose Gel Electrophoresis, Marker