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  • 79
    Thermo Fisher taqman universal pcr mm
    Taqman Universal Pcr Mm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Quantitative real-time <t>PCR</t> validation of miRNAs. To validate differential expression of the miRNAs, 4 of the miRNAs with the largest fold-changes (2 up- and 2 down-regulated; see Table 1 ), were validated using quantitative real-time PCR with either Taqman or <t>SYBR</t> Green. In addition, we performed qRT-PCR on miR-423-5p, which was the only miRNA differentially expressed at both 1 and 6 hours, and miR-466f-3p, which was chosen for subsequent investigations. If significant (FDR of 10%), miRNA fold-change on the array is given below the corresponding stretch duration. PCR results are qualitatively consistent in direction and magnitude with the array. N = 4-6/group. ANOVA and post-hoc Tukey results: *p
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    Quantitative real-time <t>PCR</t> validation of miRNAs. To validate differential expression of the miRNAs, 4 of the miRNAs with the largest fold-changes (2 up- and 2 down-regulated; see Table 1 ), were validated using quantitative real-time PCR with either Taqman or <t>SYBR</t> Green. In addition, we performed qRT-PCR on miR-423-5p, which was the only miRNA differentially expressed at both 1 and 6 hours, and miR-466f-3p, which was chosen for subsequent investigations. If significant (FDR of 10%), miRNA fold-change on the array is given below the corresponding stretch duration. PCR results are qualitatively consistent in direction and magnitude with the array. N = 4-6/group. ANOVA and post-hoc Tukey results: *p
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    Thermo Fisher taqman universal pcr master mix
    Quantitative real-time <t>PCR</t> validation of miRNAs. To validate differential expression of the miRNAs, 4 of the miRNAs with the largest fold-changes (2 up- and 2 down-regulated; see Table 1 ), were validated using quantitative real-time PCR with either Taqman or <t>SYBR</t> Green. In addition, we performed qRT-PCR on miR-423-5p, which was the only miRNA differentially expressed at both 1 and 6 hours, and miR-466f-3p, which was chosen for subsequent investigations. If significant (FDR of 10%), miRNA fold-change on the array is given below the corresponding stretch duration. PCR results are qualitatively consistent in direction and magnitude with the array. N = 4-6/group. ANOVA and post-hoc Tukey results: *p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    <t>qRT-PCR</t> validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by <t>Taqman</t> qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p
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    Quantitative real-time PCR validation of miRNAs. To validate differential expression of the miRNAs, 4 of the miRNAs with the largest fold-changes (2 up- and 2 down-regulated; see Table 1 ), were validated using quantitative real-time PCR with either Taqman or SYBR Green. In addition, we performed qRT-PCR on miR-423-5p, which was the only miRNA differentially expressed at both 1 and 6 hours, and miR-466f-3p, which was chosen for subsequent investigations. If significant (FDR of 10%), miRNA fold-change on the array is given below the corresponding stretch duration. PCR results are qualitatively consistent in direction and magnitude with the array. N = 4-6/group. ANOVA and post-hoc Tukey results: *p

    Journal: BMC Genomics

    Article Title: MicroRNA modulate alveolar epithelial response to cyclic stretch

    doi: 10.1186/1471-2164-13-154

    Figure Lengend Snippet: Quantitative real-time PCR validation of miRNAs. To validate differential expression of the miRNAs, 4 of the miRNAs with the largest fold-changes (2 up- and 2 down-regulated; see Table 1 ), were validated using quantitative real-time PCR with either Taqman or SYBR Green. In addition, we performed qRT-PCR on miR-423-5p, which was the only miRNA differentially expressed at both 1 and 6 hours, and miR-466f-3p, which was chosen for subsequent investigations. If significant (FDR of 10%), miRNA fold-change on the array is given below the corresponding stretch duration. PCR results are qualitatively consistent in direction and magnitude with the array. N = 4-6/group. ANOVA and post-hoc Tukey results: *p

    Article Snippet: Real-time PCR was performed (Taqman universal master mix or Applied Biosystems power SYBR green PCR master mix).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, SYBR Green Assay, Quantitative RT-PCR, Polymerase Chain Reaction

    qRT-PCR validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by Taqman qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p

    Journal: PLoS ONE

    Article Title: Transcriptome-wide comparison of the impact of Atoh1 and miR-183 family on pluripotent stem cells and multipotent otic progenitor cells

    doi: 10.1371/journal.pone.0180855

    Figure Lengend Snippet: qRT-PCR validation of a subset of differentially expressed genes in mESC and iMOP cells. Graphs show linear fold change of differentially expressed genes by Taqman qPCR detection in Atoh1-expressing cells (blue) and Atoh1/miR-183 family-expressing cells (red) compared to control in mESCs (A) and iMOP cells (B). Data were normalized to detection of 18S ribosomal RNA. All values represent statistically significant differences for mESCs except for Ndnf expression in Atoh1/miR-183 family-expressing cells, and statistically significant differences are indicated by asterisks for iMOP cells (n = 3, Student’s t-test p

    Article Snippet: Reverse transcription and quantitative PCR analysis (qRT-PCR) For mRNA detection, the TaqMan Reverse Transcription Kit (Life technologies) was used to reverse-transcribe RNA according to the manufacturer protocol. qPCR was performed using Taqman Fast Advanced Universal PCR Master Mix (Life Technologies), and reactions were analyzed using a 7500 Fast Real-Time PCR Instrument (Applied Biosystems).

    Techniques: Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Expressing