taqman probes Thermo Fisher Search Results


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  • 90
    Thermo Fisher taqman thermo fisher probes
    Taqman Thermo Fisher Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman mgb probe
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    Taqman Mgb Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probes hs00225039
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    Taqman Probes Hs00225039, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probes
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probe hs99999803 m1
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Taqman Probe Hs99999803 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescent probes
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Fluorescent Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman tamra probe
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Taqman Tamra Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hs04206910 cn
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Hs04206910 Cn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher aqp3 taqman probes
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Aqp3 Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mbnl3 taqman probes
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Mbnl3 Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman primers probes
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Taqman Primers Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 18s taqman probes
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    18s Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher invitrogen taqman probes
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Invitrogen Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probe hs00559657
    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with <t>TaqMan</t> probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p
    Taqman Probe Hs00559657, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman assay probes
    High-density SNP mapping in the HBB locus. (a) Distribution of 103 <t>SNPs</t> in the HBB locus interrogated by the Illumina Omni1-Quad chip and <t>TaqMan</t> assay. Globin genes are indicated by boxes. The schematic is not drawn to scale. Abbreviations: LCR, locus
    Taqman Assay Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman snp assay
    Correlation of XCI patterns obtained with the use of 3 different methods . (A) HUMARA assay versus <t>TaqMan</t> <t>SNP</t> assay at the IDS locus. For each individual, the %Sup measured by HUMARA was compared with the percentage of IDS allele obtained with the TaqMan
    Taqman Snp Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cdna taqman probes
    Correlation of XCI patterns obtained with the use of 3 different methods . (A) HUMARA assay versus <t>TaqMan</t> <t>SNP</t> assay at the IDS locus. For each individual, the %Sup measured by HUMARA was compared with the percentage of IDS allele obtained with the TaqMan
    Cdna Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman hydrolysis probes
    Correlation of XCI patterns obtained with the use of 3 different methods . (A) HUMARA assay versus <t>TaqMan</t> <t>SNP</t> assay at the IDS locus. For each individual, the %Sup measured by HUMARA was compared with the percentage of IDS allele obtained with the TaqMan
    Taqman Hydrolysis Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman primers probe combined assays
    Correlation of XCI patterns obtained with the use of 3 different methods . (A) HUMARA assay versus <t>TaqMan</t> <t>SNP</t> assay at the IDS locus. For each individual, the %Sup measured by HUMARA was compared with the percentage of IDS allele obtained with the TaqMan
    Taqman Primers Probe Combined Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher custom taqman probes
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
    Custom Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman minor groove binder
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
    Taqman Minor Groove Binder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher assays on demand taqman probes
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
    Assays On Demand Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probe collection
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
    Taqman Probe Collection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 1×taqman probe
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
    1×Taqman Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher let 7a taqman probe assay
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
    Let 7a Taqman Probe Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probe mm00468464
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
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    Thermo Fisher taqman probes spanning rs17650901
    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic <t>DNA</t> from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific <t>TaqMan</t> PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.
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    Thermo Fisher fluorescent taqman probes
    Minigene studies of α-spectrin intron 30 and the α LEPRA variant. ( A ) Partial sequence of intron 30 of the <t>SPTA1</t> gene, showing the location of the α LEPRA variant. ( B ) Each minigene construct used in minigene assays includes the ANK1 erythroid promoter, a fragment of SPTA1 genomic DNA inserted into intron 2 of the HBG1 gene, and the HBG1 3′ untranslated region and polyA signal. The hybrid HBG1-SPTA1 transcripts derived from minigenes are shown, either WT or elongated, with the locations of <t>Taqman</t> probes utilized to detect total spectrin (T bar) or the unique insert of the elongated transcript (E bar). ( C ) Minigene results. The specific sequences utilized in minigene constructs are shown. Percentages of elongated α-spectrin transcript over total α-spectrin transcript are shown in the second column from right. The adjusted P value of the difference from WT is shown on the right.
    Fluorescent Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bcl xl taqman probes
    Minigene studies of α-spectrin intron 30 and the α LEPRA variant. ( A ) Partial sequence of intron 30 of the <t>SPTA1</t> gene, showing the location of the α LEPRA variant. ( B ) Each minigene construct used in minigene assays includes the ANK1 erythroid promoter, a fragment of SPTA1 genomic DNA inserted into intron 2 of the HBG1 gene, and the HBG1 3′ untranslated region and polyA signal. The hybrid HBG1-SPTA1 transcripts derived from minigenes are shown, either WT or elongated, with the locations of <t>Taqman</t> probes utilized to detect total spectrin (T bar) or the unique insert of the elongated transcript (E bar). ( C ) Minigene results. The specific sequences utilized in minigene constructs are shown. Percentages of elongated α-spectrin transcript over total α-spectrin transcript are shown in the second column from right. The adjusted P value of the difference from WT is shown on the right.
    Bcl Xl Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman fluorogenic probes
    Minigene studies of α-spectrin intron 30 and the α LEPRA variant. ( A ) Partial sequence of intron 30 of the <t>SPTA1</t> gene, showing the location of the α LEPRA variant. ( B ) Each minigene construct used in minigene assays includes the ANK1 erythroid promoter, a fragment of SPTA1 genomic DNA inserted into intron 2 of the HBG1 gene, and the HBG1 3′ untranslated region and polyA signal. The hybrid HBG1-SPTA1 transcripts derived from minigenes are shown, either WT or elongated, with the locations of <t>Taqman</t> probes utilized to detect total spectrin (T bar) or the unique insert of the elongated transcript (E bar). ( C ) Minigene results. The specific sequences utilized in minigene constructs are shown. Percentages of elongated α-spectrin transcript over total α-spectrin transcript are shown in the second column from right. The adjusted P value of the difference from WT is shown on the right.
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    Thermo Fisher abi taqman primers probes
    Minigene studies of α-spectrin intron 30 and the α LEPRA variant. ( A ) Partial sequence of intron 30 of the <t>SPTA1</t> gene, showing the location of the α LEPRA variant. ( B ) Each minigene construct used in minigene assays includes the ANK1 erythroid promoter, a fragment of SPTA1 genomic DNA inserted into intron 2 of the HBG1 gene, and the HBG1 3′ untranslated region and polyA signal. The hybrid HBG1-SPTA1 transcripts derived from minigenes are shown, either WT or elongated, with the locations of <t>Taqman</t> probes utilized to detect total spectrin (T bar) or the unique insert of the elongated transcript (E bar). ( C ) Minigene results. The specific sequences utilized in minigene constructs are shown. Percentages of elongated α-spectrin transcript over total α-spectrin transcript are shown in the second column from right. The adjusted P value of the difference from WT is shown on the right.
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    Image Search Results


    Amplification plots of Ceratocystis platani DNA showing comparison between two different TaqMan MGB probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Amplification plots of Ceratocystis platani DNA showing comparison between two different TaqMan MGB probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques: Amplification, Fluorescence

    TaqMan MGB probes and primer specificity.

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: TaqMan MGB probes and primer specificity.

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    Ceratocystis platani detection by TaqMan MGB probe on ITS2. Selection of amplification plots from different DNA samples: (i) C. platani mycelium and infected plane tissues (continuous lines), (ii) airborne inoculum traps (AITs) from sampling area (broken

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Ceratocystis platani detection by TaqMan MGB probe on ITS2. Selection of amplification plots from different DNA samples: (i) C. platani mycelium and infected plane tissues (continuous lines), (ii) airborne inoculum traps (AITs) from sampling area (broken

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques: Selection, Amplification, Infection, Sampling

    Alignment of two primers and the TaqMan MGB probe sequences against the internal transcribed spacer (ITS) and cerato-platanin (CP) gene for which the assay was designed. Primer sequences are indicated by the arrow boxes, and the probe sequences are shown

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Alignment of two primers and the TaqMan MGB probe sequences against the internal transcribed spacer (ITS) and cerato-platanin (CP) gene for which the assay was designed. Primer sequences are indicated by the arrow boxes, and the probe sequences are shown

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    TaqMan MGB probes and primer specificity.

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: TaqMan MGB probes and primer specificity.

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    Phylogenetic analysis of nucleotide sequences from NDV strains based on a 325-bp-long fragment amplified from the fusion protein genes, which encompasses the annealing sites of oligonucleotide primers and TaqMan MGB probes described in the RRT-PCR pathotyping

    Journal: Journal of Clinical Microbiology

    Article Title: Real-Time PCR-Based Pathotyping of Newcastle Disease Virus by Use of TaqMan Minor Groove Binder Probes ▿

    doi: 10.1128/JCM.01652-08

    Figure Lengend Snippet: Phylogenetic analysis of nucleotide sequences from NDV strains based on a 325-bp-long fragment amplified from the fusion protein genes, which encompasses the annealing sites of oligonucleotide primers and TaqMan MGB probes described in the RRT-PCR pathotyping

    Article Snippet: For real-time PCR amplification, the cycling program recommended by the manufacturer of the TaqMan MGB probes (Applied Biosystems) was used: 95°C for 10 min (hot start), followed by 45 cycles of 95°C for 15 s and 60°C for 60 s. Fluorescence data were collected in the primer elongation phase at 60°C.

    Techniques: Amplification, Quantitative RT-PCR

    Schematic diagram of the principle of the ASPPAA PCR. Four allele-specific TaqMan MGB probes, corresponding to additional mismatched allele-specific primers and a common forward primer, were designed to amplify an amplicon that was as short as possible

    Journal: Applied and Environmental Microbiology

    Article Title: The Allele-Specific Probe and Primer Amplification Assay, a New Real-Time PCR Method for Fine Quantification of Single-Nucleotide Polymorphisms in Pooled DNA

    doi: 10.1128/AEM.06957-11

    Figure Lengend Snippet: Schematic diagram of the principle of the ASPPAA PCR. Four allele-specific TaqMan MGB probes, corresponding to additional mismatched allele-specific primers and a common forward primer, were designed to amplify an amplicon that was as short as possible

    Article Snippet: During the optimization steps, we assessed primer concentrations of 300, 600, and 900 nM and MGB TaqMan probe concentrations of 100, 200, and 300 nM (Applied Biosystems, Foster City, CA).

    Techniques: Polymerase Chain Reaction, Amplification

    Genomic DNA quantification with real-time TaqMan MGB probe in reticular dermis samples

    Journal: PLoS ONE

    Article Title: Glycerolized Reticular Dermis as a New Human Acellular Dermal Matrix: An Exploratory Study

    doi: 10.1371/journal.pone.0149124

    Figure Lengend Snippet: Genomic DNA quantification with real-time TaqMan MGB probe in reticular dermis samples

    Article Snippet: For absolute quantification with the real-time TaqMan MGB probe, 5 μl of 1/50 DNA diluted samples obtained from dermal matrices at different incubation times were added to a 2X Applied Biosystem “ready to use” master mix (Life Technologies, Carlsbad, CA, USA) with 500 mM of primers and 150 nM of probe and run in PCR under the same conditions as the standards.

    Techniques:

    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with TaqMan probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p

    Journal: Heliyon

    Article Title: Effects of pyrroloquinoline quinone and imidazole pyrroloquinoline on biological activities and neural functions

    doi: 10.1016/j.heliyon.2020.e03240

    Figure Lengend Snippet: Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with TaqMan probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p

    Article Snippet: Real-time quantitative polymerase chain reactions were performed in an Eco real-time PCR system (Illumia, San Diego, CA) in combination with TaqMan probes (Applied Biosystems) for COX4/1 and PGC1 α.

    Techniques: Expressing, Incubation, Cell Culture, Isolation

    RECQL5 mRNA is increased in UCC and is associated with poor prognosis Taqman qRT-PCR quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p

    Journal: Oncotarget

    Article Title: Altered RECQL5 expression in urothelial bladder carcinoma increases cellular proliferation and makes RECQL5 helicase activity a novel target for chemotherapy

    doi: 10.18632/oncotarget.12683

    Figure Lengend Snippet: RECQL5 mRNA is increased in UCC and is associated with poor prognosis Taqman qRT-PCR quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p

    Article Snippet: RT-PCR was performed using TaqMan probe and primers (Applied Biosystems, Grand Island, NY) for RECQL5β (Hs00696986_g1) and RECQL5 non isotype specific (Hs00188633_m1), Heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1) (Hs03043878_g1), Testis enhanced gene transcript protein (TEGT) (Hs01012085_m1) and Mitochondrial ATP synthase H+ transporting F1 complex beta subunit (ATP5B) (Hs00969573_mH) with TaqMan Universal PCR Mix (Applied Biosystems) as recommended by the manufacturer.

    Techniques: Quantitative RT-PCR

    Confirmation of Lpcat1 GT in ES cells and generation of mice. ( A ) Top: Diagram of Lpcat1 locus. Boxes denote exons, and intervening lines denote introns. The β-geo selection cassette encoding a β-galactosidase/neomycin fusion protein was inserted in intron 9, approximately 400 bp 3′ of exon 9. f1, f2, and r2 denote primers used to confirm the presence of GT cassette in the Lpcat1 locus by qPCR analysis. Bottom: PCR analysis of the Lpcat1 locus in GT ES cells or parental ES cells (129J/Ola) using 2 separate primer sets. The presence of amplicons in GT ES cells with the absence of signal in parental cells demonstrates trapping of the Lpcat1 locus at exon 9. Vertical line indicates discontinuous lanes in the same gel. ( B ) qPCR analysis of GT ES cells. cDNA from GT ES cells (GT) and parental cells (WT) was subjected to qPCR analysis using 3 separate exon-spanning TaqMan primer/probe sets for Lpcat1 as depicted in the bottom panel: 2/3, 9/10, and 12/13. Data were normalized to Actb and represent 3 independent experiments, each performed in triplicate. Note the difference in signal between 2/3 probe and 9/10 probe in GT cells. † P

    Journal: The Journal of Clinical Investigation

    Article Title: LPCAT1 regulates surfactant phospholipid synthesis and is required for transitioning to air breathing in mice

    doi: 10.1172/JCI38061

    Figure Lengend Snippet: Confirmation of Lpcat1 GT in ES cells and generation of mice. ( A ) Top: Diagram of Lpcat1 locus. Boxes denote exons, and intervening lines denote introns. The β-geo selection cassette encoding a β-galactosidase/neomycin fusion protein was inserted in intron 9, approximately 400 bp 3′ of exon 9. f1, f2, and r2 denote primers used to confirm the presence of GT cassette in the Lpcat1 locus by qPCR analysis. Bottom: PCR analysis of the Lpcat1 locus in GT ES cells or parental ES cells (129J/Ola) using 2 separate primer sets. The presence of amplicons in GT ES cells with the absence of signal in parental cells demonstrates trapping of the Lpcat1 locus at exon 9. Vertical line indicates discontinuous lanes in the same gel. ( B ) qPCR analysis of GT ES cells. cDNA from GT ES cells (GT) and parental cells (WT) was subjected to qPCR analysis using 3 separate exon-spanning TaqMan primer/probe sets for Lpcat1 as depicted in the bottom panel: 2/3, 9/10, and 12/13. Data were normalized to Actb and represent 3 independent experiments, each performed in triplicate. Note the difference in signal between 2/3 probe and 9/10 probe in GT cells. † P

    Article Snippet: Total RNA for qPCR was isolated and reverse transcribed into cDNA by standard methods. qPCR was performed using TaqMan primer/probe sets (Applied Biosystems) specific for Sftpc (assay ID: Mm00488144_m1), Sftpb (assay ID: Mm00455681_m1), Abca3 (assay ID: Mm00550501_m1), Lpcat1 exons 2/3 (assay ID: Mm00628177_m1), Lpcat1 exons 9/10 (assay ID: Mm00461015_m1), Lpcat1 exons 12/13 (assay ID: Mm01340302_g1), Hspa5 (assay ID: Mm00517691), Fasn (assay ID: Mm01253300_g1), and Scd1 (assay ID: Mm00772290_m1) and Actb (part number: 4352933E) as an internal control.

    Techniques: Mouse Assay, Selection, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    ATF4 expression promotes RET ubiquitination. (A and B) HEK 293T cells were transfected with the indicated plasmids, and western blot (WB) analysis was performed with the indicated antibodies. (C) Western blot analysis of TT cells infected with the lentivirus harboring ATF4 with the indicated antibodies. (D) RNA was isolated from cells treated as in (C) and subjected to real-time PCR using ATF4 or RET Taqman primers probes. (E) MTC cells expressing lentiviral-ATF4 were treated with MG132 (4 h), and denatured extracts were immunoprecipitated with an anti-RET antibody followed by western blot analysis with antiubiquitin antibody. (F) ATF4-shRNA (clones of 74 and 76) and nontargeting shRNA (Con) are treated with tunicamycin (2 μg/mL) for 24 h, and western blot analysis was performed with the indicated antibodies. (G) ATF4 expression inhibits RET signaling. MTC cells expressing lentiviral-ATF4 were immunoblotted with the indicated antibodies. IP, immunoprecipitation.

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: ATF4 Targets RET for Degradation and Is a Candidate Tumor Suppressor Gene in Medullary Thyroid Cancer

    doi: 10.1210/jc.2016-2878

    Figure Lengend Snippet: ATF4 expression promotes RET ubiquitination. (A and B) HEK 293T cells were transfected with the indicated plasmids, and western blot (WB) analysis was performed with the indicated antibodies. (C) Western blot analysis of TT cells infected with the lentivirus harboring ATF4 with the indicated antibodies. (D) RNA was isolated from cells treated as in (C) and subjected to real-time PCR using ATF4 or RET Taqman primers probes. (E) MTC cells expressing lentiviral-ATF4 were treated with MG132 (4 h), and denatured extracts were immunoprecipitated with an anti-RET antibody followed by western blot analysis with antiubiquitin antibody. (F) ATF4-shRNA (clones of 74 and 76) and nontargeting shRNA (Con) are treated with tunicamycin (2 μg/mL) for 24 h, and western blot analysis was performed with the indicated antibodies. (G) ATF4 expression inhibits RET signaling. MTC cells expressing lentiviral-ATF4 were immunoblotted with the indicated antibodies. IP, immunoprecipitation.

    Article Snippet: Total RNA and complementary DNA were prepared with use of a Cell-to-CT Kit, and quantitative real-time polymerase chain reaction (PCR) was performed with use of TaqMan primer probes (Thermo Fisher Scientific, Waltham, MA) and hypoxanthine phosphoribosyltransferase as the control.

    Techniques: Expressing, Transfection, Western Blot, Infection, Isolation, Real-time Polymerase Chain Reaction, Immunoprecipitation, shRNA

    High-density SNP mapping in the HBB locus. (a) Distribution of 103 SNPs in the HBB locus interrogated by the Illumina Omni1-Quad chip and TaqMan assay. Globin genes are indicated by boxes. The schematic is not drawn to scale. Abbreviations: LCR, locus

    Journal: Experimental Biology and Medicine

    Article Title: Original Research: A case-control genome-wide association study identifies genetic modifiers of fetal hemoglobin in sickle cell disease

    doi: 10.1177/1535370216642047

    Figure Lengend Snippet: High-density SNP mapping in the HBB locus. (a) Distribution of 103 SNPs in the HBB locus interrogated by the Illumina Omni1-Quad chip and TaqMan assay. Globin genes are indicated by boxes. The schematic is not drawn to scale. Abbreviations: LCR, locus

    Article Snippet: For the HBB locus haplotype analysis, genotype data for SNPs rs2855121 and rs2855122 were confirmed using TaqMan® assay probes (ThermoFisher Scientific, Grand Island, NY) for real-time PCR detection.

    Techniques: Chromatin Immunoprecipitation, TaqMan Assay

    Correlation of XCI patterns obtained with the use of 3 different methods . (A) HUMARA assay versus TaqMan SNP assay at the IDS locus. For each individual, the %Sup measured by HUMARA was compared with the percentage of IDS allele obtained with the TaqMan

    Journal: Blood

    Article Title: Skewing of X-inactivation ratios in blood cells of aging women is confirmed by independent methodologies

    doi: 10.1182/blood-2008-12-195677

    Figure Lengend Snippet: Correlation of XCI patterns obtained with the use of 3 different methods . (A) HUMARA assay versus TaqMan SNP assay at the IDS locus. For each individual, the %Sup measured by HUMARA was compared with the percentage of IDS allele obtained with the TaqMan

    Article Snippet: For this assay, cDNA was synthesized from PMN RNA with the use of random hexamers and TaqMan Reverse Transcription Reagents (Applied Biosystems), after which the aforementioned SNP was detected with the use of the TaqMan SNP assay (Applied Biosystems).

    Techniques:

    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic DNA from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific TaqMan PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.

    Journal: Scientific Reports

    Article Title: Loss of Sirt2 increases and prolongs a caerulein-induced pancreatitis permissive phenotype and induces spontaneous oncogenic Kras mutations in mice

    doi: 10.1038/s41598-018-34792-y

    Figure Lengend Snippet: Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic DNA from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific TaqMan PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.

    Article Snippet: Genomic DNA isolated from mouse pancreas were subjected to TaqMan PCR with custom TaqMan probes specific for mouse Kras mutation G12D or G12V (Applied Biosystems).

    Techniques: Mouse Assay, Polymerase Chain Reaction, Immunostaining, Mutagenesis, Staining

    Schematic of competition assays used to measure relative fitness levels between two strains. ( a ) Overview of life history of the standard reference N2 strain since its isolation from the wild. Derived alleles in npr-1 and glb-5 arose and fixed after 1957 and before 1969 when methods for cryopreservation were developed. These two alleles were identified for their role in changing foraging behavior on bacterial lawns from social to solitary behavior. ( b ) Schematic of pairwise competition experiments used throughout the paper to quantify fitness differences between two strains. ( c ) Relative proportion of each strain as ascertained by Droplet Digital PCR using a custom TaqMan probe (dots) is used to estimate the relative fitness between the two strains (line). ( d ) Silent mutations were edited into the 90 th or 92 nd amino acid of the dpy-10 gene using CRISPR/Cas9 to create a common SNV for Droplet Digital PCR. We refer to these as barcoded strains. ( e ) Competition experiments between the parent strain (top) and the same strain containing one of the silent mutations. We display the result from each competition experiment as a single dot overlaid on top of a boxplot showing the mean, first, and third quartiles of all replicates.

    Journal: eLife

    Article Title: Changes to social feeding behaviors are not sufficient for fitness gains of the Caenorhabditis elegans N2 reference strain

    doi: 10.7554/eLife.38675

    Figure Lengend Snippet: Schematic of competition assays used to measure relative fitness levels between two strains. ( a ) Overview of life history of the standard reference N2 strain since its isolation from the wild. Derived alleles in npr-1 and glb-5 arose and fixed after 1957 and before 1969 when methods for cryopreservation were developed. These two alleles were identified for their role in changing foraging behavior on bacterial lawns from social to solitary behavior. ( b ) Schematic of pairwise competition experiments used throughout the paper to quantify fitness differences between two strains. ( c ) Relative proportion of each strain as ascertained by Droplet Digital PCR using a custom TaqMan probe (dots) is used to estimate the relative fitness between the two strains (line). ( d ) Silent mutations were edited into the 90 th or 92 nd amino acid of the dpy-10 gene using CRISPR/Cas9 to create a common SNV for Droplet Digital PCR. We refer to these as barcoded strains. ( e ) Competition experiments between the parent strain (top) and the same strain containing one of the silent mutations. We display the result from each competition experiment as a single dot overlaid on top of a boxplot showing the mean, first, and third quartiles of all replicates.

    Article Snippet: To quantify the relative proportion of each strain, we used a digital PCR based approach using a custom TaqMan probe (Applied Biosciences).

    Techniques: Isolation, Derivative Assay, Digital PCR, CRISPR

    Minigene studies of α-spectrin intron 30 and the α LEPRA variant. ( A ) Partial sequence of intron 30 of the SPTA1 gene, showing the location of the α LEPRA variant. ( B ) Each minigene construct used in minigene assays includes the ANK1 erythroid promoter, a fragment of SPTA1 genomic DNA inserted into intron 2 of the HBG1 gene, and the HBG1 3′ untranslated region and polyA signal. The hybrid HBG1-SPTA1 transcripts derived from minigenes are shown, either WT or elongated, with the locations of Taqman probes utilized to detect total spectrin (T bar) or the unique insert of the elongated transcript (E bar). ( C ) Minigene results. The specific sequences utilized in minigene constructs are shown. Percentages of elongated α-spectrin transcript over total α-spectrin transcript are shown in the second column from right. The adjusted P value of the difference from WT is shown on the right.

    Journal: The Journal of Clinical Investigation

    Article Title: Aberrant splicing contributes to severe α-spectrin–linked congenital hemolytic anemia

    doi: 10.1172/JCI127195

    Figure Lengend Snippet: Minigene studies of α-spectrin intron 30 and the α LEPRA variant. ( A ) Partial sequence of intron 30 of the SPTA1 gene, showing the location of the α LEPRA variant. ( B ) Each minigene construct used in minigene assays includes the ANK1 erythroid promoter, a fragment of SPTA1 genomic DNA inserted into intron 2 of the HBG1 gene, and the HBG1 3′ untranslated region and polyA signal. The hybrid HBG1-SPTA1 transcripts derived from minigenes are shown, either WT or elongated, with the locations of Taqman probes utilized to detect total spectrin (T bar) or the unique insert of the elongated transcript (E bar). ( C ) Minigene results. The specific sequences utilized in minigene constructs are shown. Percentages of elongated α-spectrin transcript over total α-spectrin transcript are shown in the second column from right. The adjusted P value of the difference from WT is shown on the right.

    Article Snippet: Fluorescent Taqman probes (Applied Biosystems) corresponding to total SPTA1 and the elongated α-spectrin transcript were added to amplification reactions, allowing determination of the contribution of elongated α-spectrin transcripts after normalization.

    Techniques: Variant Assay, Sequencing, Construct, Derivative Assay