taqman probe chemistry Search Results


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  • 90
    Thermo Fisher taqman probe chemistry
    Expression changes (mRNA) of evaluation genes. A-C : Relative mRNA concentrations in the mouse neural retina were measured by qPCR (real time) for the developmental ages P2 and <t>P25.</t> Concentrations were normalized to the beta-Actin mRNA concentration. Bars indicate standard deviation for triplicate assays. <t>Taqman</t> chemistry was used for target specificity with hydrolysis probes that span exon junctions. Rho , Rcvn , Pde6b, and Sag1 are key markers of photoreceptor-specific gene expression. Genes are grouped to account for different scales of relative expression. Most genes had Pol-II peak signal ratios > 1.8, as determined from temporal Pol-II ChIP-on-Chip analysis, except for: Hdac9 (ratio 1.6), Hdac10 (ratio 1.7), and Grik2 (ratio 0.9, Table 1 ). Genes: Rhodopsin (Rho), Recoverin (Rcvrn), Retinoschisis 1 (Rs1), Phosphodiesterase 6b (Pde6b), S-antigen (Sag), Elongation of very long chain fatty acids-like 4 (Elovl4), D4, zinc and double PHD fingers, family 3 (Dpf3), Spectrin repeat containing, nuclear envelope 1 (Syne1), Solute carrier family 38, Na/H -coupled glutamine transporter, member 3 (Slc38a3), Family with sequence similarity 53, member B (A930008G19Rik, Fam53b), Nuclear receptor subfamily 1, group D, member 1 (Nr1d1), Jumonji domain containing 2C (Jmjd2c), Histone deacetylase 9 (Hdac9), Bardet-Biedl syndrome 9 (E130103I17Rik, Bbs9), Histone deacetylase 10 (Hdac10), Glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), Solute carrier family 24, Na/K/Ca exchanger, member 1 (Slc24a1).
    Taqman Probe Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher taqman probe based chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Taqman Probe Based Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman probe based chemistry/product/Thermo Fisher
    Average 88 stars, based on 250 article reviews
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    taqman probe based chemistry - by Bioz Stars, 2020-09
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    99
    Thermo Fisher taqman mgb probe chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Taqman Mgb Probe Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher taqman primer fluorogenic probe chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Taqman Primer Fluorogenic Probe Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher fluorogenic probe based taqman chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Fluorogenic Probe Based Taqman Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher predesigned gene specific taqman probe chemistries
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Predesigned Gene Specific Taqman Probe Chemistries, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher taqman hydrolysis probe chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Taqman Hydrolysis Probe Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression changes (mRNA) of evaluation genes. A-C : Relative mRNA concentrations in the mouse neural retina were measured by qPCR (real time) for the developmental ages P2 and P25. Concentrations were normalized to the beta-Actin mRNA concentration. Bars indicate standard deviation for triplicate assays. Taqman chemistry was used for target specificity with hydrolysis probes that span exon junctions. Rho , Rcvn , Pde6b, and Sag1 are key markers of photoreceptor-specific gene expression. Genes are grouped to account for different scales of relative expression. Most genes had Pol-II peak signal ratios > 1.8, as determined from temporal Pol-II ChIP-on-Chip analysis, except for: Hdac9 (ratio 1.6), Hdac10 (ratio 1.7), and Grik2 (ratio 0.9, Table 1 ). Genes: Rhodopsin (Rho), Recoverin (Rcvrn), Retinoschisis 1 (Rs1), Phosphodiesterase 6b (Pde6b), S-antigen (Sag), Elongation of very long chain fatty acids-like 4 (Elovl4), D4, zinc and double PHD fingers, family 3 (Dpf3), Spectrin repeat containing, nuclear envelope 1 (Syne1), Solute carrier family 38, Na/H -coupled glutamine transporter, member 3 (Slc38a3), Family with sequence similarity 53, member B (A930008G19Rik, Fam53b), Nuclear receptor subfamily 1, group D, member 1 (Nr1d1), Jumonji domain containing 2C (Jmjd2c), Histone deacetylase 9 (Hdac9), Bardet-Biedl syndrome 9 (E130103I17Rik, Bbs9), Histone deacetylase 10 (Hdac10), Glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), Solute carrier family 24, Na/K/Ca exchanger, member 1 (Slc24a1).

    Journal: Molecular Vision

    Article Title: Temporal ChIP-on-Chip of RNA-Polymerase-II to detect novel gene activation events during photoreceptor maturation

    doi:

    Figure Lengend Snippet: Expression changes (mRNA) of evaluation genes. A-C : Relative mRNA concentrations in the mouse neural retina were measured by qPCR (real time) for the developmental ages P2 and P25. Concentrations were normalized to the beta-Actin mRNA concentration. Bars indicate standard deviation for triplicate assays. Taqman chemistry was used for target specificity with hydrolysis probes that span exon junctions. Rho , Rcvn , Pde6b, and Sag1 are key markers of photoreceptor-specific gene expression. Genes are grouped to account for different scales of relative expression. Most genes had Pol-II peak signal ratios > 1.8, as determined from temporal Pol-II ChIP-on-Chip analysis, except for: Hdac9 (ratio 1.6), Hdac10 (ratio 1.7), and Grik2 (ratio 0.9, Table 1 ). Genes: Rhodopsin (Rho), Recoverin (Rcvrn), Retinoschisis 1 (Rs1), Phosphodiesterase 6b (Pde6b), S-antigen (Sag), Elongation of very long chain fatty acids-like 4 (Elovl4), D4, zinc and double PHD fingers, family 3 (Dpf3), Spectrin repeat containing, nuclear envelope 1 (Syne1), Solute carrier family 38, Na/H -coupled glutamine transporter, member 3 (Slc38a3), Family with sequence similarity 53, member B (A930008G19Rik, Fam53b), Nuclear receptor subfamily 1, group D, member 1 (Nr1d1), Jumonji domain containing 2C (Jmjd2c), Histone deacetylase 9 (Hdac9), Bardet-Biedl syndrome 9 (E130103I17Rik, Bbs9), Histone deacetylase 10 (Hdac10), Glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), Solute carrier family 24, Na/K/Ca exchanger, member 1 (Slc24a1).

    Article Snippet: Gene expression assays—real-time PCR Specific genes from the ChIP-on-chip data representing a full range of Pol-II peak signal ratios (P25/P2) were analyzed by real-time PCR, using Taqman probe chemistry following the manufacturer’s standard protocol (Applied Biosystems, Foster City, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Standard Deviation, Chromatin Immunoprecipitation, Sequencing, Histone Deacetylase Assay

    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by qRT-PCR using Taqman probes (white symbols).

    Journal: BMC Genomics

    Article Title: Highlights of glycosylation and adhesion related genes involved in myogenesis

    doi: 10.1186/1471-2164-15-621

    Figure Lengend Snippet: Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by qRT-PCR using Taqman probes (white symbols).

    Article Snippet: A micro-fluidic chip was used to measure quality and quantity of total RNA (Agilent 2100 Bioanalyser, Agilent Technologies Inc., Santa Clara, CA, USA) and 1 μg was converted into cDNA using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster city, CA, USA). mRNA was quantified by QRT-PCR on ABI Prism 7900 Sequence Detector System using TaqMan probe-based chemistry (Applied Biosystems), with 6-carboxyfluorescein (FAM) as a reporter. cDNA (2 ng) was used to quantify myogenic and adipogenic markers and target genes in 96-well plates and Taqman Low Density Array (TLDA) respectively.

    Techniques: Quantitative RT-PCR