taqman probe Search Results


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  • 88
    Thermo Fisher taqman probe mm00468464
    Taqman Probe Mm00468464, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probe
    RECQL5 mRNA is increased in UCC and is associated with poor prognosis <t>Taqman</t> <t>qRT-PCR</t> quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p
    Taqman Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 13813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher taqman probe hs00559657
    RECQL5 mRNA is increased in UCC and is associated with poor prognosis <t>Taqman</t> <t>qRT-PCR</t> quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p
    Taqman Probe Hs00559657, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher taqman probes hs00225039
    RECQL5 mRNA is increased in UCC and is associated with poor prognosis <t>Taqman</t> <t>qRT-PCR</t> quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p
    Taqman Probes Hs00225039, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher taqman tamra probe
    RECQL5 mRNA is increased in UCC and is associated with poor prognosis <t>Taqman</t> <t>qRT-PCR</t> quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p
    Taqman Tamra Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher taqman probe hs99999803 m1
    RECQL5 mRNA is increased in UCC and is associated with poor prognosis <t>Taqman</t> <t>qRT-PCR</t> quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p
    Taqman Probe Hs99999803 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher standard taqman probes
    RECQL5 mRNA is increased in UCC and is associated with poor prognosis <t>Taqman</t> <t>qRT-PCR</t> quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p
    Standard Taqman Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probes hs001160164 m1
    RECQL5 mRNA is increased in UCC and is associated with poor prognosis <t>Taqman</t> <t>qRT-PCR</t> quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p
    Taqman Probes Hs001160164 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mgb probes taqman
    Schematic of competition assays used to measure relative fitness levels between two strains. ( a ) Overview of life history of the standard reference N2 strain since its isolation from the wild. Derived alleles in npr-1 and glb-5 arose and fixed after 1957 and before 1969 when methods for cryopreservation were developed. These two alleles were identified for their role in changing foraging behavior on bacterial lawns from social to solitary behavior. ( b ) Schematic of pairwise competition experiments used throughout the paper to quantify fitness differences between two strains. ( c ) Relative proportion of each strain as ascertained by Droplet Digital <t>PCR</t> using a custom <t>TaqMan</t> probe (dots) is used to estimate the relative fitness between the two strains (line). ( d ) Silent mutations were edited into the 90 th or 92 nd amino acid of the dpy-10 gene using CRISPR/Cas9 to create a common SNV for Droplet Digital PCR. We refer to these as barcoded strains. ( e ) Competition experiments between the parent strain (top) and the same strain containing one of the silent mutations. We display the result from each competition experiment as a single dot overlaid on top of a boxplot showing the mean, first, and third quartiles of all replicates.
    Mgb Probes Taqman, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher taqman probes spanning rs17650901
    Schematic of competition assays used to measure relative fitness levels between two strains. ( a ) Overview of life history of the standard reference N2 strain since its isolation from the wild. Derived alleles in npr-1 and glb-5 arose and fixed after 1957 and before 1969 when methods for cryopreservation were developed. These two alleles were identified for their role in changing foraging behavior on bacterial lawns from social to solitary behavior. ( b ) Schematic of pairwise competition experiments used throughout the paper to quantify fitness differences between two strains. ( c ) Relative proportion of each strain as ascertained by Droplet Digital <t>PCR</t> using a custom <t>TaqMan</t> probe (dots) is used to estimate the relative fitness between the two strains (line). ( d ) Silent mutations were edited into the 90 th or 92 nd amino acid of the dpy-10 gene using CRISPR/Cas9 to create a common SNV for Droplet Digital PCR. We refer to these as barcoded strains. ( e ) Competition experiments between the parent strain (top) and the same strain containing one of the silent mutations. We display the result from each competition experiment as a single dot overlaid on top of a boxplot showing the mean, first, and third quartiles of all replicates.
    Taqman Probes Spanning Rs17650901, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    RECQL5 mRNA is increased in UCC and is associated with poor prognosis Taqman qRT-PCR quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p

    Journal: Oncotarget

    Article Title: Altered RECQL5 expression in urothelial bladder carcinoma increases cellular proliferation and makes RECQL5 helicase activity a novel target for chemotherapy

    doi: 10.18632/oncotarget.12683

    Figure Lengend Snippet: RECQL5 mRNA is increased in UCC and is associated with poor prognosis Taqman qRT-PCR quantification of mRNA from 197 primary bladder tumour and 20 normal tissue samples using a RECQL5 probe and plotted as fold change [ 52 ]. A. Stratified by normal compared to malignant tissue and B. according to tumour grade; * and *** indicate p

    Article Snippet: RT-PCR was performed using TaqMan probe and primers (Applied Biosystems, Grand Island, NY) for RECQL5β (Hs00696986_g1) and RECQL5 non isotype specific (Hs00188633_m1), Heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1) (Hs03043878_g1), Testis enhanced gene transcript protein (TEGT) (Hs01012085_m1) and Mitochondrial ATP synthase H+ transporting F1 complex beta subunit (ATP5B) (Hs00969573_mH) with TaqMan Universal PCR Mix (Applied Biosystems) as recommended by the manufacturer.

    Techniques: Quantitative RT-PCR

    Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with TaqMan probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p

    Journal: Heliyon

    Article Title: Effects of pyrroloquinoline quinone and imidazole pyrroloquinoline on biological activities and neural functions

    doi: 10.1016/j.heliyon.2020.e03240

    Figure Lengend Snippet: Expression of COX4/1 and PGC1α in SK-N-SH cells and HepG2 cells after incubation of the cells with PQQ, PQQH 2 and IPQ. (A) COX4/1 expression in SK-N-SH cells, (B) COX4/1 expression in HepG2 cells (C) PGC1α expression in SK-N-SH cells, (D) PGC1α expression in HepG2 cells. Total mRNAs were extracted from SK-N-SH cells and HepG2 cells that had been cultured for 24 h after the addition PQQ, PQQH 2 and IPQ. One hundred nM and 1 μM of PQQ, PQQH 2 or IPQ were added to SK-N-SH cells and HepG2 cells, respectively. Total RNAs were isolated from cultured cells. Total RNAs (3–5 μg) were transcribed into cDNA by real-time quantitative polymerase chain reactions with TaqMan probes for COX4/1 and PGC1α. The relative quantities of COX4/1 and PGC1α transcripts were normalized to the level of GAPDH message. Each value is the mean ± SEM (n = 3–5). ***p

    Article Snippet: Real-time quantitative polymerase chain reactions were performed in an Eco real-time PCR system (Illumia, San Diego, CA) in combination with TaqMan probes (Applied Biosystems) for COX4/1 and PGC1 α.

    Techniques: Expressing, Incubation, Cell Culture, Isolation

    Schematic of competition assays used to measure relative fitness levels between two strains. ( a ) Overview of life history of the standard reference N2 strain since its isolation from the wild. Derived alleles in npr-1 and glb-5 arose and fixed after 1957 and before 1969 when methods for cryopreservation were developed. These two alleles were identified for their role in changing foraging behavior on bacterial lawns from social to solitary behavior. ( b ) Schematic of pairwise competition experiments used throughout the paper to quantify fitness differences between two strains. ( c ) Relative proportion of each strain as ascertained by Droplet Digital PCR using a custom TaqMan probe (dots) is used to estimate the relative fitness between the two strains (line). ( d ) Silent mutations were edited into the 90 th or 92 nd amino acid of the dpy-10 gene using CRISPR/Cas9 to create a common SNV for Droplet Digital PCR. We refer to these as barcoded strains. ( e ) Competition experiments between the parent strain (top) and the same strain containing one of the silent mutations. We display the result from each competition experiment as a single dot overlaid on top of a boxplot showing the mean, first, and third quartiles of all replicates.

    Journal: eLife

    Article Title: Changes to social feeding behaviors are not sufficient for fitness gains of the Caenorhabditis elegans N2 reference strain

    doi: 10.7554/eLife.38675

    Figure Lengend Snippet: Schematic of competition assays used to measure relative fitness levels between two strains. ( a ) Overview of life history of the standard reference N2 strain since its isolation from the wild. Derived alleles in npr-1 and glb-5 arose and fixed after 1957 and before 1969 when methods for cryopreservation were developed. These two alleles were identified for their role in changing foraging behavior on bacterial lawns from social to solitary behavior. ( b ) Schematic of pairwise competition experiments used throughout the paper to quantify fitness differences between two strains. ( c ) Relative proportion of each strain as ascertained by Droplet Digital PCR using a custom TaqMan probe (dots) is used to estimate the relative fitness between the two strains (line). ( d ) Silent mutations were edited into the 90 th or 92 nd amino acid of the dpy-10 gene using CRISPR/Cas9 to create a common SNV for Droplet Digital PCR. We refer to these as barcoded strains. ( e ) Competition experiments between the parent strain (top) and the same strain containing one of the silent mutations. We display the result from each competition experiment as a single dot overlaid on top of a boxplot showing the mean, first, and third quartiles of all replicates.

    Article Snippet: To quantify the relative proportion of each strain, we used a digital PCR based approach using a custom TaqMan probe (Applied Biosciences).

    Techniques: Isolation, Derivative Assay, Digital PCR, CRISPR

    Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic DNA from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific TaqMan PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.

    Journal: Scientific Reports

    Article Title: Loss of Sirt2 increases and prolongs a caerulein-induced pancreatitis permissive phenotype and induces spontaneous oncogenic Kras mutations in mice

    doi: 10.1038/s41598-018-34792-y

    Figure Lengend Snippet: Accumulation of spontaneous tumorigenic Kras mutations in Sirt2 -deficient mice. (a) Accumulation of tumorigenic Kras mutations during inflammation-coupled neoplasm. Mouse pancreas genomic DNA from wild-type and Sirt2 KO, with and without caerulein-treatment, were analyzed for Kras G12D or Kras G12V mutations by competitive allele-specific TaqMan PCR. The table shows the summary of analysis for percentage of mice positive for mutations (46 wild-type mice, 56 Sirt2 KO mice) after pancreatitis. (b) Detail description of wild-type and Sirt2 KO mice with Kras G12D and Kras G12V mutations. (c) Bar graph representation of ( b ). ( d ) Immunostaining detection of KRAS-G12D mutant protein in mouse pancreas. Mice pancreas tissues were fixed, embedded in paraffin, and immuno-histochemical staining with anti- KRAS-G12D antibody was performed. Representative images are shown. Bars indicate 100 µm.

    Article Snippet: Genomic DNA isolated from mouse pancreas were subjected to TaqMan PCR with custom TaqMan probes specific for mouse Kras mutation G12D or G12V (Applied Biosystems).

    Techniques: Mouse Assay, Polymerase Chain Reaction, Immunostaining, Mutagenesis, Staining

    Amplification plots of Ceratocystis platani DNA showing comparison between two different TaqMan MGB probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Amplification plots of Ceratocystis platani DNA showing comparison between two different TaqMan MGB probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques: Amplification, Fluorescence

    TaqMan MGB probes and primer specificity.

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: TaqMan MGB probes and primer specificity.

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    Ceratocystis platani detection by TaqMan MGB probe on ITS2. Selection of amplification plots from different DNA samples: (i) C. platani mycelium and infected plane tissues (continuous lines), (ii) airborne inoculum traps (AITs) from sampling area (broken

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Ceratocystis platani detection by TaqMan MGB probe on ITS2. Selection of amplification plots from different DNA samples: (i) C. platani mycelium and infected plane tissues (continuous lines), (ii) airborne inoculum traps (AITs) from sampling area (broken

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques: Selection, Amplification, Infection, Sampling

    Alignment of two primers and the TaqMan MGB probe sequences against the internal transcribed spacer (ITS) and cerato-platanin (CP) gene for which the assay was designed. Primer sequences are indicated by the arrow boxes, and the probe sequences are shown

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Alignment of two primers and the TaqMan MGB probe sequences against the internal transcribed spacer (ITS) and cerato-platanin (CP) gene for which the assay was designed. Primer sequences are indicated by the arrow boxes, and the probe sequences are shown

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    TaqMan MGB probes and primer specificity.

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: TaqMan MGB probes and primer specificity.

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    Phylogenetic analysis of nucleotide sequences from NDV strains based on a 325-bp-long fragment amplified from the fusion protein genes, which encompasses the annealing sites of oligonucleotide primers and TaqMan MGB probes described in the RRT-PCR pathotyping

    Journal: Journal of Clinical Microbiology

    Article Title: Real-Time PCR-Based Pathotyping of Newcastle Disease Virus by Use of TaqMan Minor Groove Binder Probes ▿

    doi: 10.1128/JCM.01652-08

    Figure Lengend Snippet: Phylogenetic analysis of nucleotide sequences from NDV strains based on a 325-bp-long fragment amplified from the fusion protein genes, which encompasses the annealing sites of oligonucleotide primers and TaqMan MGB probes described in the RRT-PCR pathotyping

    Article Snippet: For real-time PCR amplification, the cycling program recommended by the manufacturer of the TaqMan MGB probes (Applied Biosystems) was used: 95°C for 10 min (hot start), followed by 45 cycles of 95°C for 15 s and 60°C for 60 s. Fluorescence data were collected in the primer elongation phase at 60°C.

    Techniques: Amplification, Quantitative RT-PCR