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    Thermo Fisher taqman master mix
    Inflammatory cytokine and Wnt mRNA levels in primary human 3D lung aggregate cultures and human macrophages (M) after cigarette smoke extracts (CSE) exposure. (A) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and CSE exposed (48 h) lung aggregate cultures, containing and not containing M. (B) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and rWnt5a (1 µg/ml) treated lung aggregate cultures, containing and not containing M. (C) <t>Taqman</t> array heat map analysis of pooled ( n = 4) <t>cDNA</t> of human M compared with control. M were treated with CSE for 3 h. (D) Changes in mRNA levels of Wnt signaling pathway genes measured by Taqman Array analysis using pooled ( n = 4) cDNA samples of human M compared with control.
    Taqman Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman master mix/product/Thermo Fisher
    Average 99 stars, based on 5368 article reviews
    Price from $9.99 to $1999.99
    taqman master mix - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher taqman preamp master mix kit
    Relative levels of mmu-miR-466h and its target genes in fresh and nutrient-depleted media with and without mmu-miR-466h inhibition. (A) Mmu-miR-466h levels. <t>TaqMan</t> microRNA <t>qRT-PCR</t> analysis was used to assess mmu-miR-466h levels at 23.5h in different
    Taqman Preamp Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman preamp master mix kit/product/Thermo Fisher
    Average 97 stars, based on 1463 article reviews
    Price from $9.99 to $1999.99
    taqman preamp master mix kit - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Inflammatory cytokine and Wnt mRNA levels in primary human 3D lung aggregate cultures and human macrophages (M) after cigarette smoke extracts (CSE) exposure. (A) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and CSE exposed (48 h) lung aggregate cultures, containing and not containing M. (B) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and rWnt5a (1 µg/ml) treated lung aggregate cultures, containing and not containing M. (C) Taqman array heat map analysis of pooled ( n = 4) cDNA of human M compared with control. M were treated with CSE for 3 h. (D) Changes in mRNA levels of Wnt signaling pathway genes measured by Taqman Array analysis using pooled ( n = 4) cDNA samples of human M compared with control.

    Journal: Frontiers in Immunology

    Article Title: Cigarette Smoke-Induced Pulmonary Inflammation Becomes Systemic by Circulating Extracellular Vesicles Containing Wnt5a and Inflammatory Cytokines

    doi: 10.3389/fimmu.2018.01724

    Figure Lengend Snippet: Inflammatory cytokine and Wnt mRNA levels in primary human 3D lung aggregate cultures and human macrophages (M) after cigarette smoke extracts (CSE) exposure. (A) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and CSE exposed (48 h) lung aggregate cultures, containing and not containing M. (B) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and rWnt5a (1 µg/ml) treated lung aggregate cultures, containing and not containing M. (C) Taqman array heat map analysis of pooled ( n = 4) cDNA of human M compared with control. M were treated with CSE for 3 h. (D) Changes in mRNA levels of Wnt signaling pathway genes measured by Taqman Array analysis using pooled ( n = 4) cDNA samples of human M compared with control.

    Article Snippet: TaqMan master mix was combined with the cDNA samples then the mixed solutions were loaded onto the Human WNT Pathway, Fast 96-well TaqMan Array (Thermo Fisher Scientific, Waltham, MA, USA) plate.

    Techniques:

    Correlation of measurements obtained by qPCR and ddPCR. Plots showing the correlation (with 95% confidence interval) between microRNA quantification performed by single TaqMan assays (= qPCR) and ddPCR. In plot A, D and G the target microRNAs are normalized to cel-miR-39, in plot B and E miR-16 was used for normalization, and in plot C and F data were normalized to the mean of cel-miR-39 and miR-16.

    Journal: PLoS ONE

    Article Title: Quantification of microRNA levels in plasma – Impact of preanalytical and analytical conditions

    doi: 10.1371/journal.pone.0201069

    Figure Lengend Snippet: Correlation of measurements obtained by qPCR and ddPCR. Plots showing the correlation (with 95% confidence interval) between microRNA quantification performed by single TaqMan assays (= qPCR) and ddPCR. In plot A, D and G the target microRNAs are normalized to cel-miR-39, in plot B and E miR-16 was used for normalization, and in plot C and F data were normalized to the mean of cel-miR-39 and miR-16.

    Article Snippet: The reaction was performed using 3 μL of purified microRNA in a total volume of 10 μL, and the mixture was incubated in 40 cycles of 16°C for 2 min, 42°C for 1 min and 50°C for 1 sec, followed by 1 cycle of 85°C for 5 min. As recommended by the manufacturer, a pre-amplification step was performed using 5 μL of the transcribed cDNA in a total reaction volume of 25 μL containing TaqMan®PreAmp Master Mix and Custom TaqMan MIR PreAmp Pool (Applied Biosystems).

    Techniques: Real-time Polymerase Chain Reaction

    Validation of the 41 classifier genes using TaqMan real-time PCR assays. The expression profile of the 41 classifier genes was measured in each of the 82 samples by real-time PCR using TaqMan® Gene Expression Assays. Based on TaqMan assay data, the coefficient of the 41 classifier genes were re-learned from the 52 training samples and used to classify the status of 30 testing samples using the same method applied to microarray data. (A). Classification probabilities for each testing sample: TAA (case) vs. normal (control); (B). Contingency table summarizes the predicted and actual class membership for the testing set; (C). Classification accuracy, sensitivity and specificity.

    Journal: PLoS ONE

    Article Title: Gene Expression Signature in Peripheral Blood Detects Thoracic Aortic Aneurysm

    doi: 10.1371/journal.pone.0001050

    Figure Lengend Snippet: Validation of the 41 classifier genes using TaqMan real-time PCR assays. The expression profile of the 41 classifier genes was measured in each of the 82 samples by real-time PCR using TaqMan® Gene Expression Assays. Based on TaqMan assay data, the coefficient of the 41 classifier genes were re-learned from the 52 training samples and used to classify the status of 30 testing samples using the same method applied to microarray data. (A). Classification probabilities for each testing sample: TAA (case) vs. normal (control); (B). Contingency table summarizes the predicted and actual class membership for the testing set; (C). Classification accuracy, sensitivity and specificity.

    Article Snippet: The resulting cDNA was subjected to a 14-cycle PCR amplification followed by real-time PCR reaction using the manufacturer's TaqMan® PreAmp Master Mix Kit Protocol (Applied Biosystems, PN 4366127).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, TaqMan Assay, Microarray

    Relative levels of mmu-miR-466h and its target genes in fresh and nutrient-depleted media with and without mmu-miR-466h inhibition. (A) Mmu-miR-466h levels. TaqMan microRNA qRT-PCR analysis was used to assess mmu-miR-466h levels at 23.5h in different

    Journal: Biotechnology and bioengineering

    Article Title: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells

    doi: 10.1002/bit.23092

    Figure Lengend Snippet: Relative levels of mmu-miR-466h and its target genes in fresh and nutrient-depleted media with and without mmu-miR-466h inhibition. (A) Mmu-miR-466h levels. TaqMan microRNA qRT-PCR analysis was used to assess mmu-miR-466h levels at 23.5h in different

    Article Snippet: The preamplification (10 cycles) was done using Applied Systems TaqMan® PreAmp Kit (Part No. 4384267) in PCR Thermal cycler (Applied Biosystems) after miRNA reverse transcription and before qRT-PCR reads.

    Techniques: Inhibition, Quantitative RT-PCR

    qRT-PCR comparison of mmu-miR-466h, mmu-miR-669c, and mRNA levels for mmu-miR-466h predicted targets in fresh and depleted media after 24h. (A) Mmu-miR-466h and mmu-miR-669c levels. The TaqMan microRNA assays were used for both miRs with sno202 and let-7c

    Journal: Biotechnology and bioengineering

    Article Title: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells

    doi: 10.1002/bit.23092

    Figure Lengend Snippet: qRT-PCR comparison of mmu-miR-466h, mmu-miR-669c, and mRNA levels for mmu-miR-466h predicted targets in fresh and depleted media after 24h. (A) Mmu-miR-466h and mmu-miR-669c levels. The TaqMan microRNA assays were used for both miRs with sno202 and let-7c

    Article Snippet: The preamplification (10 cycles) was done using Applied Systems TaqMan® PreAmp Kit (Part No. 4384267) in PCR Thermal cycler (Applied Biosystems) after miRNA reverse transcription and before qRT-PCR reads.

    Techniques: Quantitative RT-PCR