taqman mgb probe Search Results


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  • 99
    Thermo Fisher taqman mgb probe
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    Taqman Mgb Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher locus specific taqman minor groove binder probes
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    Locus Specific Taqman Minor Groove Binder Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher 5 nuclease taqman minor groove binder minor groove binder mgb probes
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    5 Nuclease Taqman Minor Groove Binder Minor Groove Binder Mgb Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman minor groove binder probe bacr p
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    Taqman Minor Groove Binder Probe Bacr P, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher minor groove binder labeled taqman probe
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    Minor Groove Binder Labeled Taqman Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman mgb probe assay
    Amplification plots of Ceratocystis platani DNA showing comparison between two different <t>TaqMan</t> <t>MGB</t> probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence
    Taqman Mgb Probe Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher taqman minor groove binder
    Unique DNA sequence in the Rickettsia japonica genome that analyzed PCR in this study. A) Comparative genome map of the 216-bp open reading frame <t>(ORF).</t> The R. japonica –specific sequence region (AB437281) in the R. japonica genome and the complete genome sequence of R. conorii strain Malish 7 were compared. The RC1338 DNA sequence and the mapping position data for R. conorii were obtained from the Rickettsia genome database ( www.igs.cnrs-mrs.fr/mgdb/Rickettsia ). Two solid black arrows indicate primer positions; this region was amplified and sequenced with the same primers. B) Alignments of R. japonica –specific 216-bp ORF (AB437281) between R. japonica YH, R. heilongjiangensis CH8-1, and Rickettsia sp. LON, as performed by the program ClustalW ( www.ebi.ac.uk/clustalw ), and the positions of primers and probe in real-time PCR. Primer positions and directions (black arrows) and the <t>TaqMan</t> minor groove binder (MGB) (line) probe position are shown. The 216-bp ORF of R. heilongjiangensis and Rickettsia sp. LON were registered on GenBank with accession nos. AB512783 and AB512784, respectively. DNA sequences are identical among R. japonica strains and Rickettsia sp. LON strains (asterisks). The alignment was edited with BioEdit version 7.0.0 ( www.mbio.ncsu.edu/BioEdit/bioedit.html ).
    Taqman Minor Groove Binder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Amplification plots of Ceratocystis platani DNA showing comparison between two different TaqMan MGB probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Amplification plots of Ceratocystis platani DNA showing comparison between two different TaqMan MGB probes. The fluorescence (normalized reporter signal, Rn) of the TaqMan MGB probes for the CP target gene (broken line) appears later than the fluorescence

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques: Amplification, Fluorescence

    TaqMan MGB probes and primer specificity.

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: TaqMan MGB probes and primer specificity.

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    Ceratocystis platani detection by TaqMan MGB probe on ITS2. Selection of amplification plots from different DNA samples: (i) C. platani mycelium and infected plane tissues (continuous lines), (ii) airborne inoculum traps (AITs) from sampling area (broken

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Ceratocystis platani detection by TaqMan MGB probe on ITS2. Selection of amplification plots from different DNA samples: (i) C. platani mycelium and infected plane tissues (continuous lines), (ii) airborne inoculum traps (AITs) from sampling area (broken

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques: Selection, Amplification, Infection, Sampling

    Alignment of two primers and the TaqMan MGB probe sequences against the internal transcribed spacer (ITS) and cerato-platanin (CP) gene for which the assay was designed. Primer sequences are indicated by the arrow boxes, and the probe sequences are shown

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: Alignment of two primers and the TaqMan MGB probe sequences against the internal transcribed spacer (ITS) and cerato-platanin (CP) gene for which the assay was designed. Primer sequences are indicated by the arrow boxes, and the probe sequences are shown

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    TaqMan MGB probes and primer specificity.

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

    doi: 10.1128/AEM.01484-13

    Figure Lengend Snippet: TaqMan MGB probes and primer specificity.

    Article Snippet: The primer and probe final concentrations used were as follows: 300 nM forward primer (Eurofins MWG Operon, Ebersberg, Germany), 300 nM reverse primer (Eurofins MWG Operon), and 200 nM TaqMan MGB probe (Applied Biosystems) designed for amplification of ITS and CP genes.

    Techniques:

    Phylogenetic analysis of nucleotide sequences from NDV strains based on a 325-bp-long fragment amplified from the fusion protein genes, which encompasses the annealing sites of oligonucleotide primers and TaqMan MGB probes described in the RRT-PCR pathotyping

    Journal: Journal of Clinical Microbiology

    Article Title: Real-Time PCR-Based Pathotyping of Newcastle Disease Virus by Use of TaqMan Minor Groove Binder Probes ▿

    doi: 10.1128/JCM.01652-08

    Figure Lengend Snippet: Phylogenetic analysis of nucleotide sequences from NDV strains based on a 325-bp-long fragment amplified from the fusion protein genes, which encompasses the annealing sites of oligonucleotide primers and TaqMan MGB probes described in the RRT-PCR pathotyping

    Article Snippet: For real-time PCR amplification, the cycling program recommended by the manufacturer of the TaqMan MGB probes (Applied Biosystems) was used: 95°C for 10 min (hot start), followed by 45 cycles of 95°C for 15 s and 60°C for 60 s. Fluorescence data were collected in the primer elongation phase at 60°C.

    Techniques: Amplification, Quantitative RT-PCR

    Identification of 4 novel genes during murine pancreatic development. Expression of Cbs, Gfi1, Meis1, and Aldh18a1 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous GAPDH was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p

    Journal: Pancreas

    Article Title: Synergizing Genomic Analysis with Biological Knowledge to Identify and Validate Novel Genes in Pancreatic Development

    doi: 10.1097/MPA.0b013e31823d0160

    Figure Lengend Snippet: Identification of 4 novel genes during murine pancreatic development. Expression of Cbs, Gfi1, Meis1, and Aldh18a1 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous GAPDH was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p

    Article Snippet: Assays used FAM dye labeled Taqman MGB probes and an ABI 7000 PCR instrument (Applied Biosystems, Foster City, CA) and were normalized to glyceraldehyde-3-phosphate-dehydrogenase ( gapdh ) as previously published .

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Synthesized, Nuclease Assay, Labeling, Polymerase Chain Reaction

    Expression of mature endocrine markers; Ins, Gcg, Sst, Iapp, Pcsk1 and Pcsk2 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous gapdh was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p

    Journal: Pancreas

    Article Title: Synergizing Genomic Analysis with Biological Knowledge to Identify and Validate Novel Genes in Pancreatic Development

    doi: 10.1097/MPA.0b013e31823d0160

    Figure Lengend Snippet: Expression of mature endocrine markers; Ins, Gcg, Sst, Iapp, Pcsk1 and Pcsk2 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous gapdh was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p

    Article Snippet: Assays used FAM dye labeled Taqman MGB probes and an ABI 7000 PCR instrument (Applied Biosystems, Foster City, CA) and were normalized to glyceraldehyde-3-phosphate-dehydrogenase ( gapdh ) as previously published .

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Synthesized, Nuclease Assay, Labeling, Polymerase Chain Reaction

    Temporal expression of Pdx1, Ptf1a, Neurog3 , Hes1 , Sox9 and Rfx6 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous gapdh was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p

    Journal: Pancreas

    Article Title: Synergizing Genomic Analysis with Biological Knowledge to Identify and Validate Novel Genes in Pancreatic Development

    doi: 10.1097/MPA.0b013e31823d0160

    Figure Lengend Snippet: Temporal expression of Pdx1, Ptf1a, Neurog3 , Hes1 , Sox9 and Rfx6 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous gapdh was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p

    Article Snippet: Assays used FAM dye labeled Taqman MGB probes and an ABI 7000 PCR instrument (Applied Biosystems, Foster City, CA) and were normalized to glyceraldehyde-3-phosphate-dehydrogenase ( gapdh ) as previously published .

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Synthesized, Nuclease Assay, Labeling, Polymerase Chain Reaction

    Unique DNA sequence in the Rickettsia japonica genome that analyzed PCR in this study. A) Comparative genome map of the 216-bp open reading frame (ORF). The R. japonica –specific sequence region (AB437281) in the R. japonica genome and the complete genome sequence of R. conorii strain Malish 7 were compared. The RC1338 DNA sequence and the mapping position data for R. conorii were obtained from the Rickettsia genome database ( www.igs.cnrs-mrs.fr/mgdb/Rickettsia ). Two solid black arrows indicate primer positions; this region was amplified and sequenced with the same primers. B) Alignments of R. japonica –specific 216-bp ORF (AB437281) between R. japonica YH, R. heilongjiangensis CH8-1, and Rickettsia sp. LON, as performed by the program ClustalW ( www.ebi.ac.uk/clustalw ), and the positions of primers and probe in real-time PCR. Primer positions and directions (black arrows) and the TaqMan minor groove binder (MGB) (line) probe position are shown. The 216-bp ORF of R. heilongjiangensis and Rickettsia sp. LON were registered on GenBank with accession nos. AB512783 and AB512784, respectively. DNA sequences are identical among R. japonica strains and Rickettsia sp. LON strains (asterisks). The alignment was edited with BioEdit version 7.0.0 ( www.mbio.ncsu.edu/BioEdit/bioedit.html ).

    Journal: Emerging Infectious Diseases

    Article Title: Diagnostic Assay for Rickettsia japonica

    doi: 10.3201/eid1512.090252

    Figure Lengend Snippet: Unique DNA sequence in the Rickettsia japonica genome that analyzed PCR in this study. A) Comparative genome map of the 216-bp open reading frame (ORF). The R. japonica –specific sequence region (AB437281) in the R. japonica genome and the complete genome sequence of R. conorii strain Malish 7 were compared. The RC1338 DNA sequence and the mapping position data for R. conorii were obtained from the Rickettsia genome database ( www.igs.cnrs-mrs.fr/mgdb/Rickettsia ). Two solid black arrows indicate primer positions; this region was amplified and sequenced with the same primers. B) Alignments of R. japonica –specific 216-bp ORF (AB437281) between R. japonica YH, R. heilongjiangensis CH8-1, and Rickettsia sp. LON, as performed by the program ClustalW ( www.ebi.ac.uk/clustalw ), and the positions of primers and probe in real-time PCR. Primer positions and directions (black arrows) and the TaqMan minor groove binder (MGB) (line) probe position are shown. The 216-bp ORF of R. heilongjiangensis and Rickettsia sp. LON were registered on GenBank with accession nos. AB512783 and AB512784, respectively. DNA sequences are identical among R. japonica strains and Rickettsia sp. LON strains (asterisks). The alignment was edited with BioEdit version 7.0.0 ( www.mbio.ncsu.edu/BioEdit/bioedit.html ).

    Article Snippet: Therefore, we focused on this conserved region of the 216-bp ORF to develop a TaqMan minor groove binder (MGB) probe (Applied Biosystems) that could detect the pathogenic R. japonica group, including R. heilongjiangensis , with a high degree of specificity.

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction