Journal: The Journal of Neuroscience
Article Title: Loss of Retrograde Endocannabinoid Signaling and Reduced Adult Neurogenesis in Diacylglycerol Lipase Knock-out Mice
Figure Lengend Snippet: Generation and validation of DAGLβ −/− mice. a , DAGLβ knock-out mice were generated from a Lexicon OmniBank ES cell clone OST195261, which contains a gene trap cassette insertion in the first exon of the mouse DAGLβ gene. LTR, Long terminal repeat; NEO, neomycin gene; pA, polyadenylation sequence; SV40tpA, SV40 triple polyadenylation sequence; PGK, phosphoglycerate kinase-1 promoter; BTK, Bruton tyrosine kinase. b , TaqMan analysis for DAGLβ mRNA levels in the cortex (CTX), cerebellum (Cb), spinal cord (SC), and liver (LI) from wt (+/+), DAGLβ +/− (+/−), and DAGLβ −/− (−/−) mice ( n = 4/genotype). DAGLβ expression is normalized as the percentage of GAPDH levels. Error bars indicate SEM. c , Western blot analysis of cerebellum tissues from wt (WT), DAGLα −/− (α −/− ), and DAGLβ −/− (β −/− ) mice demonstrates the absence of DAGLβ protein in DAGLβ −/− mice.
Article Snippet: The TaqMan gene expression IDs for DAGLβ, MAGL, FAAH, CB1 , and CB2 are Mm01201462_m1, Mm00449274_m1, Mm00515684_m1, Mm01212171_ s1, and Mm00438286_m1 (Applied Biosystems).
Techniques: Mouse Assay, Knock-Out, Generated, Sequencing, Expressing, Western Blot