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    Thermo Fisher taqman gene expression mastermix thermo fisher scientific
    Taqman Gene Expression Mastermix Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman gene expression mastermix thermo fisher scientific/product/Thermo Fisher
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    taqman gene expression mastermix thermo fisher scientific - by Bioz Stars, 2020-07
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    99
    Thermo Fisher gene expression taqman gene expression assays
    <t>qPCR</t> analysis of dorsal root ganglions (DRGs) from scLRP1 −/− and scLRP1 +/+ mice show upregulation of regeneration associated genes (RAGs) before and after injury <t>Taqman</t> qPCR analysis of ( A ) uninjured DRGs showing CREB3, ATF3, STAT3 and SPRR1A in scLRP1 −/− mice (n=3–5 mice per genotype; *p
    Gene Expression Taqman Gene Expression Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12045 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene expression taqman gene expression assays/product/Thermo Fisher
    Average 99 stars, based on 12045 article reviews
    Price from $9.99 to $1999.99
    gene expression taqman gene expression assays - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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    qPCR analysis of dorsal root ganglions (DRGs) from scLRP1 −/− and scLRP1 +/+ mice show upregulation of regeneration associated genes (RAGs) before and after injury Taqman qPCR analysis of ( A ) uninjured DRGs showing CREB3, ATF3, STAT3 and SPRR1A in scLRP1 −/− mice (n=3–5 mice per genotype; *p

    Journal: Glia

    Article Title: Schwann Cells Regulate Sensory Neuron Gene Expression Before and After Peripheral Nerve Injury

    doi: 10.1002/glia.23325

    Figure Lengend Snippet: qPCR analysis of dorsal root ganglions (DRGs) from scLRP1 −/− and scLRP1 +/+ mice show upregulation of regeneration associated genes (RAGs) before and after injury Taqman qPCR analysis of ( A ) uninjured DRGs showing CREB3, ATF3, STAT3 and SPRR1A in scLRP1 −/− mice (n=3–5 mice per genotype; *p

    Article Snippet: RNA was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using TaqMan® gene expression products and an AB Step one Plus Real-Time PCR System (Applied Biosystems), as previously described ( ; ).

    Techniques: Real-time Polymerase Chain Reaction, Mouse Assay

    Validation of microarray gene expression by real‐time PCR. A–D: Relative expression levels of genes identified by SAM; FOXA1, TRAF3IP3, HCST and CYP1B1, measured by TaqMan qRT‐PCR in 22 type II and 6 type I DCIS cases. E–G: Relative expression level of EMT‐related genes identified by SAM; SNAI1, S100A8 and CXCL1, measured by TaqMan qRT‐PCR in 22 DCIS type II and 6 DCIS type I cases. Black horizontal bars represent median value for each DCIS phenotype.

    Journal: Molecular Oncology

    Article Title: Molecular diversity in ductal carcinoma in situ (DCIS) and early invasive breast cancer), Molecular diversity in ductal carcinoma in situ (DCIS) and early invasive breast cancer

    doi: 10.1016/j.molonc.2010.06.007

    Figure Lengend Snippet: Validation of microarray gene expression by real‐time PCR. A–D: Relative expression levels of genes identified by SAM; FOXA1, TRAF3IP3, HCST and CYP1B1, measured by TaqMan qRT‐PCR in 22 type II and 6 type I DCIS cases. E–G: Relative expression level of EMT‐related genes identified by SAM; SNAI1, S100A8 and CXCL1, measured by TaqMan qRT‐PCR in 22 DCIS type II and 6 DCIS type I cases. Black horizontal bars represent median value for each DCIS phenotype.

    Article Snippet: cDNA was synthesised in a total volume of 20 μl with 100 ng total RNA using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA) and used as template for real‐time PCR analysis in TaqMan Gene Expression Assays (Applied Biosystems) on an ABI Prism 7900HT sequence detector system (Applied Biosystems).

    Techniques: Microarray, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Hypoxia-induced modification of PLK promoter methylation in HCC cells. (a) Promoter methylation status of the plks examined in HCC-derived cells HepG2 and Hep3B; U = unmethylated, M = methylated. Fully methylated HeLa DNA was used as a positive control (+M), no template was added to the negative control (−M). (b) Post hypoxia, PLK4 transcripts were assessed via qPCR in RNA extracted from HCC cells. All qPCR data is representative of the mean value of three independent experiments and error bars represent +/− SD. (c) PLK protein levels were examined post treatment from whole cell lysates. Actin was used as a loading control. (−) represents lysates from untreated cells, (+) lysates from cells grown in the presence of hypoxia. (d) Quantification of protein levels using densitometry. Levels have been normalized to the respective untreated controls. Data is representative of the mean value of three independent experiments and error bars represent +/− SD. (e) The fold change of PLK1 transcripts as determined by qPCR. Values normalized to the respective untreated sample. (f) PLK2 and PLK3 analyzed and fold changed determine by normalization to the respective untreated samples. (g) Hif1α transcripts post hypoxia were determine by real-time PCR using a Taqman probe.

    Journal: PLoS ONE

    Article Title: p53-Dependent and Cell Specific Epigenetic Regulation of the Polo-like kinases under Oxidative Stress

    doi: 10.1371/journal.pone.0087918

    Figure Lengend Snippet: Hypoxia-induced modification of PLK promoter methylation in HCC cells. (a) Promoter methylation status of the plks examined in HCC-derived cells HepG2 and Hep3B; U = unmethylated, M = methylated. Fully methylated HeLa DNA was used as a positive control (+M), no template was added to the negative control (−M). (b) Post hypoxia, PLK4 transcripts were assessed via qPCR in RNA extracted from HCC cells. All qPCR data is representative of the mean value of three independent experiments and error bars represent +/− SD. (c) PLK protein levels were examined post treatment from whole cell lysates. Actin was used as a loading control. (−) represents lysates from untreated cells, (+) lysates from cells grown in the presence of hypoxia. (d) Quantification of protein levels using densitometry. Levels have been normalized to the respective untreated controls. Data is representative of the mean value of three independent experiments and error bars represent +/− SD. (e) The fold change of PLK1 transcripts as determined by qPCR. Values normalized to the respective untreated sample. (f) PLK2 and PLK3 analyzed and fold changed determine by normalization to the respective untreated samples. (g) Hif1α transcripts post hypoxia were determine by real-time PCR using a Taqman probe.

    Article Snippet: Real time PCRs were carried out on an ABI 7300 machine using Taqman gene expression probes for mouse Plk1 , Plk4 , and HIF1α ; and human PLK1-PLK4 , and HIF1α (Applied biosystems).

    Techniques: Modification, Methylation, Derivative Assay, Positive Control, Negative Control, Real-time Polymerase Chain Reaction

    MAML1 promotes Runx2-mediated osteoblastic differentiation. A, C3H10T1/2 cells were transiently transfected with a Runx2 and/or MAML1 expression plasmids and cultured for 2 days. B, Total RNA was isolated and reverse-transcribed and TaqMan real-time PCR was performed to investigate the expression level of alkaline phosphatase gene, a marker of osteoblast.

    Journal: PLoS Genetics

    Article Title: MAML1 Enhances the Transcriptional Activity of Runx2 and Plays a Role in Bone Development

    doi: 10.1371/journal.pgen.1003132

    Figure Lengend Snippet: MAML1 promotes Runx2-mediated osteoblastic differentiation. A, C3H10T1/2 cells were transiently transfected with a Runx2 and/or MAML1 expression plasmids and cultured for 2 days. B, Total RNA was isolated and reverse-transcribed and TaqMan real-time PCR was performed to investigate the expression level of alkaline phosphatase gene, a marker of osteoblast.

    Article Snippet: Real-time PCR was performed using a TaqMan Gene Expression Assay, TaqMan Universal PCR Mix and the 7900HT Fast Real-Time PCR System (Applied Biosystems).

    Techniques: Transfection, Expressing, Cell Culture, Isolation, Real-time Polymerase Chain Reaction, Marker

    Altered miRNAs in neonatal vs. adult mouse lung. A : Venn diagram of altered miRNAs in neonatal vs. adult lungs. Fifty miRNAs increased more than twofold, and 17 miRNAs decreased by more than half in neonates exposed to hyperoxia. In adults exposed to hyperoxia, 21 miRNAs increased more than twofold, and 12 miRNAs decreased by more than half. B : this Venn diagram of miRNA related to Bach1 shows no altered miRNAs common to either neonatal or adult lungs exposed to hyperoxia on the basis of array data. C : selected miRNAs from the miRNA expression profile were validated by RT-quantitative PCR (qPCR). D : correlation between data from microarray and RT-PCR for selected miRNAs was performed using lineal regression. The x -axis represents fold in log 2 by qPCR; the y -axis represents fold in log 2 by TaqMan low-density array data.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: MiR-196a regulates heme oxygenase-1 by silencing Bach1 in the neonatal mouse lung

    doi: 10.1152/ajplung.00428.2015

    Figure Lengend Snippet: Altered miRNAs in neonatal vs. adult mouse lung. A : Venn diagram of altered miRNAs in neonatal vs. adult lungs. Fifty miRNAs increased more than twofold, and 17 miRNAs decreased by more than half in neonates exposed to hyperoxia. In adults exposed to hyperoxia, 21 miRNAs increased more than twofold, and 12 miRNAs decreased by more than half. B : this Venn diagram of miRNA related to Bach1 shows no altered miRNAs common to either neonatal or adult lungs exposed to hyperoxia on the basis of array data. C : selected miRNAs from the miRNA expression profile were validated by RT-quantitative PCR (qPCR). D : correlation between data from microarray and RT-PCR for selected miRNAs was performed using lineal regression. The x -axis represents fold in log 2 by qPCR; the y -axis represents fold in log 2 by TaqMan low-density array data.

    Article Snippet: Steady-state mRNA and miRNA levels were evaluated by quantitative real-time PCR using a TaqMan gene expression system (Applied Biosystems).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Microarray, Reverse Transcription Polymerase Chain Reaction, TLDA Assay

    Generation and validation of DAGLβ −/− mice. a , DAGLβ knock-out mice were generated from a Lexicon OmniBank ES cell clone OST195261, which contains a gene trap cassette insertion in the first exon of the mouse DAGLβ gene. LTR, Long terminal repeat; NEO, neomycin gene; pA, polyadenylation sequence; SV40tpA, SV40 triple polyadenylation sequence; PGK, phosphoglycerate kinase-1 promoter; BTK, Bruton tyrosine kinase. b , TaqMan analysis for DAGLβ mRNA levels in the cortex (CTX), cerebellum (Cb), spinal cord (SC), and liver (LI) from wt (+/+), DAGLβ +/− (+/−), and DAGLβ −/− (−/−) mice ( n = 4/genotype). DAGLβ expression is normalized as the percentage of GAPDH levels. Error bars indicate SEM. c , Western blot analysis of cerebellum tissues from wt (WT), DAGLα −/− (α −/− ), and DAGLβ −/− (β −/− ) mice demonstrates the absence of DAGLβ protein in DAGLβ −/− mice.

    Journal: The Journal of Neuroscience

    Article Title: Loss of Retrograde Endocannabinoid Signaling and Reduced Adult Neurogenesis in Diacylglycerol Lipase Knock-out Mice

    doi: 10.1523/JNEUROSCI.5693-09.2010

    Figure Lengend Snippet: Generation and validation of DAGLβ −/− mice. a , DAGLβ knock-out mice were generated from a Lexicon OmniBank ES cell clone OST195261, which contains a gene trap cassette insertion in the first exon of the mouse DAGLβ gene. LTR, Long terminal repeat; NEO, neomycin gene; pA, polyadenylation sequence; SV40tpA, SV40 triple polyadenylation sequence; PGK, phosphoglycerate kinase-1 promoter; BTK, Bruton tyrosine kinase. b , TaqMan analysis for DAGLβ mRNA levels in the cortex (CTX), cerebellum (Cb), spinal cord (SC), and liver (LI) from wt (+/+), DAGLβ +/− (+/−), and DAGLβ −/− (−/−) mice ( n = 4/genotype). DAGLβ expression is normalized as the percentage of GAPDH levels. Error bars indicate SEM. c , Western blot analysis of cerebellum tissues from wt (WT), DAGLα −/− (α −/− ), and DAGLβ −/− (β −/− ) mice demonstrates the absence of DAGLβ protein in DAGLβ −/− mice.

    Article Snippet: The TaqMan gene expression IDs for DAGLβ, MAGL, FAAH, CB1 , and CB2 are Mm01201462_m1, Mm00449274_m1, Mm00515684_m1, Mm01212171_ s1, and Mm00438286_m1 (Applied Biosystems).

    Techniques: Mouse Assay, Knock-Out, Generated, Sequencing, Expressing, Western Blot

    Generation and validation of DAGLα −/− mice. a , Targeting strategy for DAGLα: genomic structure of the mouse DAGLα gene up to exon 8. Targeting vector replaces a portion of exon 1 with a selection cassette. Homologous integration is detected by Southern blotting using probes external to the targeting vector on both the 5′ and 3′ sides. Probes are indicated as solid lines above the genomic locus. Both probes detect an endogenous band of 16.4 kb in NdeI-cut genomic DNA. Homologous targeting creates bands of 8.6 and 11.1 kb when probed with 5′ or 3′ external probes, respectively. b , TaqMan analysis for DAGLα mRNA levels in cortex (CTX), cerebellum (Cb), and spinal cord (SC) from wt (+/+), DAGLα +/− (+/−), and DAGLα −/− (−/−) mice ( n = 3–5/genotype). DAGLα expression is normalized as the percentage of GAPDH levels. Error bars indicate SEM. c , Western blot analysis of cerebellum tissues from wt (WT), DAGLα −/− (α −/− ), and DAGLβ −/− (β −/− ) mice demonstrates the absence of DAGLα protein in DAGLα −/− mice. Expression of actin in each sample is used as a loading control. In d , DAGLα expression is restricted to Purkinje cell dendrites in the cerebellum of adult wt mice (the red staining), with this staining lost in DAGLα −/− mice ( e ). Normal staining of GFAP, to highlight Bergman glial cells, was found in the wt and DAGLα −/− mice (the green staining in d and e ). The sections were counterstained to show cell nuclei (in blue).

    Journal: The Journal of Neuroscience

    Article Title: Loss of Retrograde Endocannabinoid Signaling and Reduced Adult Neurogenesis in Diacylglycerol Lipase Knock-out Mice

    doi: 10.1523/JNEUROSCI.5693-09.2010

    Figure Lengend Snippet: Generation and validation of DAGLα −/− mice. a , Targeting strategy for DAGLα: genomic structure of the mouse DAGLα gene up to exon 8. Targeting vector replaces a portion of exon 1 with a selection cassette. Homologous integration is detected by Southern blotting using probes external to the targeting vector on both the 5′ and 3′ sides. Probes are indicated as solid lines above the genomic locus. Both probes detect an endogenous band of 16.4 kb in NdeI-cut genomic DNA. Homologous targeting creates bands of 8.6 and 11.1 kb when probed with 5′ or 3′ external probes, respectively. b , TaqMan analysis for DAGLα mRNA levels in cortex (CTX), cerebellum (Cb), and spinal cord (SC) from wt (+/+), DAGLα +/− (+/−), and DAGLα −/− (−/−) mice ( n = 3–5/genotype). DAGLα expression is normalized as the percentage of GAPDH levels. Error bars indicate SEM. c , Western blot analysis of cerebellum tissues from wt (WT), DAGLα −/− (α −/− ), and DAGLβ −/− (β −/− ) mice demonstrates the absence of DAGLα protein in DAGLα −/− mice. Expression of actin in each sample is used as a loading control. In d , DAGLα expression is restricted to Purkinje cell dendrites in the cerebellum of adult wt mice (the red staining), with this staining lost in DAGLα −/− mice ( e ). Normal staining of GFAP, to highlight Bergman glial cells, was found in the wt and DAGLα −/− mice (the green staining in d and e ). The sections were counterstained to show cell nuclei (in blue).

    Article Snippet: The TaqMan gene expression IDs for DAGLβ, MAGL, FAAH, CB1 , and CB2 are Mm01201462_m1, Mm00449274_m1, Mm00515684_m1, Mm01212171_ s1, and Mm00438286_m1 (Applied Biosystems).

    Techniques: Mouse Assay, Plasmid Preparation, Selection, Southern Blot, Expressing, Western Blot, Staining