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    Thermo Fisher pcr master mix
    Results of PPARγ mRNA expression analysis with <t>TaqMan</t> <t>RT-PCR</t> from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.
    Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman universal pcr master mix
    Results of PPARγ mRNA expression analysis with <t>TaqMan</t> <t>RT-PCR</t> from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.
    Taqman Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54679 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 54679 article reviews
    Price from $9.99 to $1999.99
    taqman universal pcr master mix - by Bioz Stars, 2020-08
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    Thermo Fisher fast advanced taqman universal pcr master mix
    <t>CCL20</t> and CCR6 gene expression in colonic biopsies. A and B: Microarray gene expression results of CCL20 and CCR6 in colonic biopsies from healthy controls (N), active (UCa) or inactive (UCi) ulcerative colitis, and active (CDa) or inactive (CDi) Crohn’s disease. Individual values (Log 2 ) and mean are plotted. C and D: <t>qRT-PCR</t> gene expression results of CCL20 and CCR6 in colonic biopsies from N, Ulcerative Colitis (UC) and Crohn’s disease (CD), n = 5 in each group. Individual values (foldchange 2 -ΔΔCt ) and mean are plotted. *p
    Fast Advanced Taqman Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast advanced taqman universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 399 article reviews
    Price from $9.99 to $1999.99
    fast advanced taqman universal pcr master mix - by Bioz Stars, 2020-08
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    Results of PPARγ mRNA expression analysis with TaqMan RT-PCR from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.

    Journal: International Journal of Molecular Sciences

    Article Title: PPARγ Expression Is Diminished in Macrophages of Recurrent Miscarriage Placentas

    doi: 10.3390/ijms19071872

    Figure Lengend Snippet: Results of PPARγ mRNA expression analysis with TaqMan RT-PCR from trophoblastic tissue. PPARγ mRNA expression was significantly downregulated in the miscarriage groups (SM, 15 cases, p = 0.01) and RM, 16 cases, p = 0.004)) compared to the healthy controls (15 cases). This bar graph shows the mean of relative PPARγ expression; therefore, the presentation of error bars is not appropriate.

    Article Snippet: Each reaction was accomplished with a volume of 20 µL, including 1 µL cDNA, 8 µL H2 O (DEPC treated DI water; Sigma, Taufkirchen, Germany) and 10 µL TaqMan® Fast Universal PCR Master Mix 2× (Applied Biosystems, Nr.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Comparison of miRNA expression levels obtained by NGS Illumina sequencing vs. TaqMan PCR. The results of the correlation are visualized as bar diagrams on the left and scatter diagrams with regression lines on the right comparing next generation sequencing (blue/y-axis) and TaqMan fold changes (red/x-axis) for hsa-miR-378a ( A ), hsa-miR-375 ( B ), hsa-miR-21-5p ( C ) and hsa-miR-215-5p ( D ). There was a good correlation of the NGS with the TaqMan results with a strong relationship for hsa-miR-378a (Pearson’s r = 0.9), hsa-miR-375 ( r = 0.77) and hsa-miR-215-5p ( r = 0.75), but only a moderate relationship for hsa-miR-21-5p ( r = 0.53).

    Journal: Oncotarget

    Article Title: Extensive screening of microRNA populations identifies hsa-miR-375 and hsa-miR-133a-3p as selective markers for human rectal and colon cancer

    doi: 10.18632/oncotarget.25535

    Figure Lengend Snippet: Comparison of miRNA expression levels obtained by NGS Illumina sequencing vs. TaqMan PCR. The results of the correlation are visualized as bar diagrams on the left and scatter diagrams with regression lines on the right comparing next generation sequencing (blue/y-axis) and TaqMan fold changes (red/x-axis) for hsa-miR-378a ( A ), hsa-miR-375 ( B ), hsa-miR-21-5p ( C ) and hsa-miR-215-5p ( D ). There was a good correlation of the NGS with the TaqMan results with a strong relationship for hsa-miR-378a (Pearson’s r = 0.9), hsa-miR-375 ( r = 0.77) and hsa-miR-215-5p ( r = 0.75), but only a moderate relationship for hsa-miR-21-5p ( r = 0.53).

    Article Snippet: Total RNA was transcribed to cDNA using the TaqMan® MicroRNA Reverse Transcription Kit (Applied Biosystems, Catalog # 4366597). cDNAs were amplified using the TaqMan® Fast Universal PCR Master Mix (2X), no AmpErase® UNG (Applied Biosystems, Catalog # 4352042).

    Techniques: Expressing, Next-Generation Sequencing, Sequencing, Polymerase Chain Reaction

    Depletion of CCR2 + cells impairs CD4 + T cell activation and leads to the death of fbp1 Δ mutant yeast-infected mice. CCR2-DTR mice and control (DTR-negative) littermates were treated with 250 ng of DT intraperitoneally before and after infection with 10 6 fbp1 Δ mutant cells as illustrated in panel A. (B) Survival curve of fbp1 Δ mutant yeast - infected, CCR2-depleted mice (dashed red line) and control littermates (solid red line). The data shown are cumulative from three independent experiments with four or five mice per group. ****, P ≤ 0.0001 (determined by log rank [Mantel-Cox] test). (C) Total number of CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Each symbol represents one mouse. The data shown are cumulative from two experiments with four or five mice per group ***, P ≤ 0.001 (determined by Mann-Whitney test). (D) Representative FACS profile of cytokine production by CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice or control littermates. Plots are gated on Thy1.2 + CD4 + CD8 − lymphocytes in BALF. (E) CFU counts in lung tissue of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Data are cumulative from two independent experiments with three or four mice per group. ***, P ≤ 0.001 (determined by Mann-Whitney test). (F) Cytokine gene transcription in lung tissue was examined on day 6 after infection with the fbp1 Δ mutant in CCR2-depleted mice (red bars) and control littermates (red striped bars). Control C57BL/6J mice infected with H99 were also analyzed as a control population (black bars). Differential gene expression relative to GAPDH was examined by qRT-PCR with cytokine-specific TaqMan probes and calculated by the ΔΔ CT method. The data shown are cumulative from two independent experiments with four mice per group and are depicted as mean ± the standard error of the mean. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Journal: mBio

    Article Title: The F-Box Protein Fbp1 Shapes the Immunogenic Potential of Cryptococcus neoformans

    doi: 10.1128/mBio.01828-17

    Figure Lengend Snippet: Depletion of CCR2 + cells impairs CD4 + T cell activation and leads to the death of fbp1 Δ mutant yeast-infected mice. CCR2-DTR mice and control (DTR-negative) littermates were treated with 250 ng of DT intraperitoneally before and after infection with 10 6 fbp1 Δ mutant cells as illustrated in panel A. (B) Survival curve of fbp1 Δ mutant yeast - infected, CCR2-depleted mice (dashed red line) and control littermates (solid red line). The data shown are cumulative from three independent experiments with four or five mice per group. ****, P ≤ 0.0001 (determined by log rank [Mantel-Cox] test). (C) Total number of CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Each symbol represents one mouse. The data shown are cumulative from two experiments with four or five mice per group ***, P ≤ 0.001 (determined by Mann-Whitney test). (D) Representative FACS profile of cytokine production by CD4 + T cells recovered from the BALF of fbp1 Δ mutant - infected, CCR2-depleted mice or control littermates. Plots are gated on Thy1.2 + CD4 + CD8 − lymphocytes in BALF. (E) CFU counts in lung tissue of fbp1 Δ mutant - infected, CCR2-depleted mice (red symbols) and control littermates (black symbols) on day 6 after infection. Data are cumulative from two independent experiments with three or four mice per group. ***, P ≤ 0.001 (determined by Mann-Whitney test). (F) Cytokine gene transcription in lung tissue was examined on day 6 after infection with the fbp1 Δ mutant in CCR2-depleted mice (red bars) and control littermates (red striped bars). Control C57BL/6J mice infected with H99 were also analyzed as a control population (black bars). Differential gene expression relative to GAPDH was examined by qRT-PCR with cytokine-specific TaqMan probes and calculated by the ΔΔ CT method. The data shown are cumulative from two independent experiments with four mice per group and are depicted as mean ± the standard error of the mean. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Article Snippet: TaqMan Fast Universal PCR master mix (2×) No Amp and TaqMan probes (Applied Biosystems) for each gene were used and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

    Techniques: Activation Assay, Mutagenesis, Infection, Mouse Assay, MANN-WHITNEY, FACS, Expressing, Quantitative RT-PCR

    Effective maturation of CCR2 + Ly6C + monocytes into mo-DCs after fbp1 Δ mutant yeast infection. Differentiation of mo-DCs was analyzed on day 3 after i.n. infection with H99 (black bars) or the fbp1 Δ mutant (red bars). The data shown are cumulative from two independent experiments with four or five mice per group and are depicted as the mean ± the standard error of the mean. (A) Representative FACS profile of Ly6C + monocyte maturation into mo-DCs (defined as CD45 + CD11b + Ly6C hi Ly6G-CD11c + class II + ) in H99- or fbp1 Δ mutant-infected mice. MHC, major histocompatibility complex. (B) Percentages of mo-DCs (CD11c + class II + ) in monocyte gate (CD45 + CD11b + Ly6C hi Ly6G − ) in mice infected with H99 (black bar) or the fbp1 Δ mutant (red). (C) Total numbers of mo-DCs recruited to the lungs. (D to F) Total numbers of recruited neutrophils (D), monocytes (E), and mo-DCs (F) on day 3 after infection with H99 (black symbols), the fbp1 Δ mutant (red symbols), or the complemented strain ( fbp1 Δ FBP1 ) (gray symbols). (G to I) Chemokine expression in lung tissue was analyzed by qRT-PCR. The expression of each CCR2 ligand was examined with TaqMan probes. Differential gene expression relative to GAPDH was calculated by the ΔΔ CT method. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Journal: mBio

    Article Title: The F-Box Protein Fbp1 Shapes the Immunogenic Potential of Cryptococcus neoformans

    doi: 10.1128/mBio.01828-17

    Figure Lengend Snippet: Effective maturation of CCR2 + Ly6C + monocytes into mo-DCs after fbp1 Δ mutant yeast infection. Differentiation of mo-DCs was analyzed on day 3 after i.n. infection with H99 (black bars) or the fbp1 Δ mutant (red bars). The data shown are cumulative from two independent experiments with four or five mice per group and are depicted as the mean ± the standard error of the mean. (A) Representative FACS profile of Ly6C + monocyte maturation into mo-DCs (defined as CD45 + CD11b + Ly6C hi Ly6G-CD11c + class II + ) in H99- or fbp1 Δ mutant-infected mice. MHC, major histocompatibility complex. (B) Percentages of mo-DCs (CD11c + class II + ) in monocyte gate (CD45 + CD11b + Ly6C hi Ly6G − ) in mice infected with H99 (black bar) or the fbp1 Δ mutant (red). (C) Total numbers of mo-DCs recruited to the lungs. (D to F) Total numbers of recruited neutrophils (D), monocytes (E), and mo-DCs (F) on day 3 after infection with H99 (black symbols), the fbp1 Δ mutant (red symbols), or the complemented strain ( fbp1 Δ FBP1 ) (gray symbols). (G to I) Chemokine expression in lung tissue was analyzed by qRT-PCR. The expression of each CCR2 ligand was examined with TaqMan probes. Differential gene expression relative to GAPDH was calculated by the ΔΔ CT method. **, P ≤ 0.01 (determined by Mann-Whitney test).

    Article Snippet: TaqMan Fast Universal PCR master mix (2×) No Amp and TaqMan probes (Applied Biosystems) for each gene were used and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

    Techniques: Mutagenesis, Infection, Mouse Assay, FACS, Expressing, Quantitative RT-PCR, MANN-WHITNEY

    CCL20 and CCR6 gene expression in colonic biopsies. A and B: Microarray gene expression results of CCL20 and CCR6 in colonic biopsies from healthy controls (N), active (UCa) or inactive (UCi) ulcerative colitis, and active (CDa) or inactive (CDi) Crohn’s disease. Individual values (Log 2 ) and mean are plotted. C and D: qRT-PCR gene expression results of CCL20 and CCR6 in colonic biopsies from N, Ulcerative Colitis (UC) and Crohn’s disease (CD), n = 5 in each group. Individual values (foldchange 2 -ΔΔCt ) and mean are plotted. *p

    Journal: PLoS ONE

    Article Title: Expression of CCL20 and Its Corresponding Receptor CCR6 Is Enhanced in Active Inflammatory Bowel Disease, and TLR3 Mediates CCL20 Expression in Colonic Epithelial Cells

    doi: 10.1371/journal.pone.0141710

    Figure Lengend Snippet: CCL20 and CCR6 gene expression in colonic biopsies. A and B: Microarray gene expression results of CCL20 and CCR6 in colonic biopsies from healthy controls (N), active (UCa) or inactive (UCi) ulcerative colitis, and active (CDa) or inactive (CDi) Crohn’s disease. Individual values (Log 2 ) and mean are plotted. C and D: qRT-PCR gene expression results of CCL20 and CCR6 in colonic biopsies from N, Ulcerative Colitis (UC) and Crohn’s disease (CD), n = 5 in each group. Individual values (foldchange 2 -ΔΔCt ) and mean are plotted. *p

    Article Snippet: For CCL20 , a TaqMan assay was used with the Fast Real-Time PCR Universal PCR Master Mix and TaqMan probes (probe ID Hs01011368_m1, Life Technologies, CA, USA).

    Techniques: Expressing, Microarray, Quantitative RT-PCR