taq polymerase Takara Search Results


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  • 90
    Thermo Fisher taq dna polymerase
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 33236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 33236 article reviews
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    taq dna polymerase - by Bioz Stars, 2020-02
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    86
    Thermo Fisher takara taq polymerase
    Takara Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 18 article reviews
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    takara taq polymerase - by Bioz Stars, 2020-02
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    90
    TaKaRa z taq polymerase
    Optimization of the <t>UP-M-PCR.</t> Lane A, B, C, D, E, amplicon fragments by UP (500 nmol L −1 ) and compound specific primer hpt-839, nptII-508, pat-262, bar-226 and sps-110 at a series concentrations of 500 nmol L −1 , 50 nmol L −1 , 25 nmol L −1 , 5 nmol L −1 , 0.5 nmol L −1 ; lane F1, amplicon fragments by UP at 500 nmol L −1 and all compound specific primers at 25 nmol L −1 ; lane F2, amplicon fragments by UP at 500 nmol L −1 and all compound specific primers at the optimized concentration; lane G1,G2,G3, amplicon fragments by all primers at the optimized concentration with TaKaRa <t>Taq</t> ™, Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase; lane H1, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the common amplification conditions; lane H2, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the optimized amplification conditions; lane M, 100 bp DNA Marker.
    Z Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 2719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    z taq polymerase - by Bioz Stars, 2020-02
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    TaKaRa taq dna polymerase
    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic <t>DNA.</t> PCR was performed on the isolated genomic DNA using <t>taq</t> DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for
    Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 9958 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 9958 article reviews
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    taq dna polymerase - by Bioz Stars, 2020-02
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    TaKaRa la taq polymerase
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
    La Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 4252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 4252 article reviews
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    la taq polymerase - by Bioz Stars, 2020-02
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    TaKaRa ex taq dna polymerase
    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with <t>Taq</t> I. Lane M: 100 bp <t>DNA</t> ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
    Ex Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 7119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 7119 article reviews
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    93
    PerkinElmer taq polymerase
    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with <t>Taq</t> I. Lane M: 100 bp <t>DNA</t> ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
    Taq Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 4139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore takara taq polymerase
    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with <t>Taq</t> I. Lane M: 100 bp <t>DNA</t> ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
    Takara Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 4 article reviews
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    takara taq polymerase - by Bioz Stars, 2020-02
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    New England Biolabs takara taq polymerase
    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with <t>Taq</t> I. Lane M: 100 bp <t>DNA</t> ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
    Takara Taq Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    takara taq polymerase - by Bioz Stars, 2020-02
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    Fisher Scientific takara taq polymerase
    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with <t>Taq</t> I. Lane M: 100 bp <t>DNA</t> ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
    Takara Taq Polymerase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa titanium taq dna pol
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Titanium Taq Dna Pol, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa hot start dna polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Hot Start Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hot start dna polymerase - by Bioz Stars, 2020-02
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    TaKaRa premix taq dna polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Premix Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 255 article reviews
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    premix taq dna polymerase - by Bioz Stars, 2020-02
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    Thermo Fisher taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 25147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr premix ex taq kits
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Sybr Premix Ex Taq Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa extaq hot start version
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Extaq Hot Start Version, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa hotstar taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Hotstar Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hotstar taq polymerase - by Bioz Stars, 2020-02
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    79
    TaKaRa primerstar taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Primerstar Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa gxl taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Gxl Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa takaraex taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Takaraex Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    takaraex taq polymerase - by Bioz Stars, 2020-02
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    96
    Bioneer Corporation taq dna polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Taq Dna Polymerase, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 96/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Fisher Scientific ex taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Ex Taq Polymerase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore la taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    La Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 21 article reviews
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    la taq polymerase - by Bioz Stars, 2020-02
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    92
    Shimadzu Corporation taq dna polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Taq Dna Polymerase, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    TaKaRa exo taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Exo Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    TaKaRa platinium taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Platinium Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore ex taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Ex Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Roche taq dna polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Taq Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 6706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pyrobest taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Pyrobest Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    TaKaRa klen taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Klen Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa faststart taq polymerase
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Faststart Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Optimization of the UP-M-PCR. Lane A, B, C, D, E, amplicon fragments by UP (500 nmol L −1 ) and compound specific primer hpt-839, nptII-508, pat-262, bar-226 and sps-110 at a series concentrations of 500 nmol L −1 , 50 nmol L −1 , 25 nmol L −1 , 5 nmol L −1 , 0.5 nmol L −1 ; lane F1, amplicon fragments by UP at 500 nmol L −1 and all compound specific primers at 25 nmol L −1 ; lane F2, amplicon fragments by UP at 500 nmol L −1 and all compound specific primers at the optimized concentration; lane G1,G2,G3, amplicon fragments by all primers at the optimized concentration with TaKaRa Taq ™, Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase; lane H1, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the common amplification conditions; lane H2, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the optimized amplification conditions; lane M, 100 bp DNA Marker.

    Journal: PLoS ONE

    Article Title: A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis

    doi: 10.1371/journal.pone.0022900

    Figure Lengend Snippet: Optimization of the UP-M-PCR. Lane A, B, C, D, E, amplicon fragments by UP (500 nmol L −1 ) and compound specific primer hpt-839, nptII-508, pat-262, bar-226 and sps-110 at a series concentrations of 500 nmol L −1 , 50 nmol L −1 , 25 nmol L −1 , 5 nmol L −1 , 0.5 nmol L −1 ; lane F1, amplicon fragments by UP at 500 nmol L −1 and all compound specific primers at 25 nmol L −1 ; lane F2, amplicon fragments by UP at 500 nmol L −1 and all compound specific primers at the optimized concentration; lane G1,G2,G3, amplicon fragments by all primers at the optimized concentration with TaKaRa Taq ™, Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase; lane H1, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the common amplification conditions; lane H2, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the optimized amplification conditions; lane M, 100 bp DNA Marker.

    Article Snippet: To test the efficiency of Taq Polymerase to be employed in PCR assays, comparative tests were made with several Taq polymerases, such as Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase, and TaKaRa Taq ™.

    Techniques: Polymerase Chain Reaction, Amplification, Concentration Assay, Marker

    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region

    Journal: Heredity

    Article Title: Deficiency of a pyrroline-5-carboxylate reductase produces the yellowish green cocoon ‘Ryokuken’ of the silkworm, Bombyx mori

    doi: 10.1038/s41437-018-0051-8

    Figure Lengend Snippet: Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region

    Article Snippet: The region from transcription initiation to terminator (1F-9063R) and to the 1st intronic region on the Daizo genome (1F-5924R) of BmP5CR1 was amplified by 2.5 U Ex Taq polymerase or LA Taq polymerase (TaKaRa, Japan).

    Techniques: Polymerase Chain Reaction, Marker

    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Journal: Neuroscience letters

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain

    doi: 10.1016/j.neulet.2010.12.060

    Figure Lengend Snippet: (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Article Snippet: PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′).

    Techniques: Mutagenesis, Mouse Assay, Polymerase Chain Reaction, Isolation

    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio). PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Journal: Nucleic Acids Research

    Article Title: Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

    doi: 10.1093/nar/gni111

    Figure Lengend Snippet: PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio). PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Article Snippet: The same results were obtained by the other polymerase systems: LA Taq polymerase (Takara-bio), Tth polymerase (Applied-boisystems); Expand High Fidelity, Expand High FidelityPLUS and Expand Long Template polymerase (Roche-diagnostics); TITANIUM Taq polymerase (Becton-Dickinson-Clontech); and Taq polymerase (Promega).

    Techniques: Polymerase Chain Reaction, Genomic Sequencing, Sequencing, Staining

    PCR with primers carrying mismatched bases. PCR was performed at two human genomic sites with primers (20 bp), one of which (forward primer) carried one, two or three mismatched bases in the middle of the primer, in the absence (left) or presence (right) of Tth RecA protein and ATP using the Taq DNA polymerase ( rTaq DNA polymerase plus ‘hot start’ antibody). ( a ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 66 562 and nt 66 581 in GenBank accession no AC006454 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set a-1); lanes 2 and 6, PCR products using primers (primer set a-2 with one mismatched base at nt 66 566, T to C); lanes 3 and 7, PCR products using primers (primer set a-3 with two mismatched base at nt 66 566 and nt 66 571, both T to C); and lanes 4 and 8, PCR products using primers (primer set a-4 with three mismatched base at nt 66 566 and nt 66 571, T to C and nt 66 576, G to C). The oligonucleotide sequences used for the forward primers (mismatched bases are underlined) are as follows: primer set a-1, 5′-CATGGCACCTGCTCTGAGAC-3′; primer set a-2, 5′-CATGGCACC C GCTCTGAGAC-3′; primer set a-3, 5′-CATGGCACC C GCTC C GAGAC-3′; and primer set a-4, 5′-CATG C CACC C GCTC C GAGAC-3′. ( b ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 38 501 and nt 38 520 in GenBank accession no. AC0937734 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set b-1); lanes 2 and 6, PCR products using primers (primer set b-2 with one mismatched base at nt 38 505, G to A); lanes 3 and 7, PCR products using primers (primer set b-3 with two mismatched base at nt 38 505 and nt 38 510, both G to A); and lanes 4 and 8, PCR products using primers (primer set b-4 with three mismatched base at nt 38 505, nt 38 510 and nt 38 515, all G to A). The oligonucleotide sequences used for the forward primers are as follows: primer set b-1, 5′-ATCTGTGTGGTTCGGCTCTG-3′; primer set b-2, 5′-ATCTGTGTG A TTCGGCTCTG-3′; primer set b-3, 5′-ATCTGTGTG A TTCG A CTCTG-3′; and primer set b-4, 5′-ATCT A TGTG A TTCG A CTCTG-3′. ( c ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 63 957 and nt 63 976 in GenBank accession no. AC004975 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set c-1); lanes 2 and 6, PCR products using primers (primer set c-2 with one mismatched base at nt 63 961, A to T); lanes 3 and 7, PCR products using primers (primer set c-3 with two mismatched base at nt 63 961 and nt 63 966, A to T and C to T); and lanes 4 and 8, PCR products using primers (primer set c-4 with three mismatched base at nt 63 961, nt 63 966 and nt 63 971, A to T, C to T and G to T). The oligonucleotide sequences used for the forward primers are as follows: primer set c-1, 5′-GCAGGCACCAAGAACTACTG-3′; primer set c-2, 5′-GCAGGCACC T AGAACTACTG-3′; primer set c-3, 5′-GCAGGCACC T AGAA T TACTG-3′; and primer set c-4, 5′-GCAG T CACC T AGAA T TACTG-3′. The sequences for the backward primers are 5′-TCACCTCCCAGCCTGGCCCA-3′ for ( a ), 5′-AGGGAGATGTTCTCATAAAT-3′ and 5′-CTGTAAGTGGCAGACATTAC-3′ for ( b ). Nucleotide numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Journal: Nucleic Acids Research

    Article Title: Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

    doi: 10.1093/nar/gni111

    Figure Lengend Snippet: PCR with primers carrying mismatched bases. PCR was performed at two human genomic sites with primers (20 bp), one of which (forward primer) carried one, two or three mismatched bases in the middle of the primer, in the absence (left) or presence (right) of Tth RecA protein and ATP using the Taq DNA polymerase ( rTaq DNA polymerase plus ‘hot start’ antibody). ( a ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 66 562 and nt 66 581 in GenBank accession no AC006454 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set a-1); lanes 2 and 6, PCR products using primers (primer set a-2 with one mismatched base at nt 66 566, T to C); lanes 3 and 7, PCR products using primers (primer set a-3 with two mismatched base at nt 66 566 and nt 66 571, both T to C); and lanes 4 and 8, PCR products using primers (primer set a-4 with three mismatched base at nt 66 566 and nt 66 571, T to C and nt 66 576, G to C). The oligonucleotide sequences used for the forward primers (mismatched bases are underlined) are as follows: primer set a-1, 5′-CATGGCACCTGCTCTGAGAC-3′; primer set a-2, 5′-CATGGCACC C GCTCTGAGAC-3′; primer set a-3, 5′-CATGGCACC C GCTC C GAGAC-3′; and primer set a-4, 5′-CATG C CACC C GCTC C GAGAC-3′. ( b ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 38 501 and nt 38 520 in GenBank accession no. AC0937734 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set b-1); lanes 2 and 6, PCR products using primers (primer set b-2 with one mismatched base at nt 38 505, G to A); lanes 3 and 7, PCR products using primers (primer set b-3 with two mismatched base at nt 38 505 and nt 38 510, both G to A); and lanes 4 and 8, PCR products using primers (primer set b-4 with three mismatched base at nt 38 505, nt 38 510 and nt 38 515, all G to A). The oligonucleotide sequences used for the forward primers are as follows: primer set b-1, 5′-ATCTGTGTGGTTCGGCTCTG-3′; primer set b-2, 5′-ATCTGTGTG A TTCGGCTCTG-3′; primer set b-3, 5′-ATCTGTGTG A TTCG A CTCTG-3′; and primer set b-4, 5′-ATCT A TGTG A TTCG A CTCTG-3′. ( c ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 63 957 and nt 63 976 in GenBank accession no. AC004975 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set c-1); lanes 2 and 6, PCR products using primers (primer set c-2 with one mismatched base at nt 63 961, A to T); lanes 3 and 7, PCR products using primers (primer set c-3 with two mismatched base at nt 63 961 and nt 63 966, A to T and C to T); and lanes 4 and 8, PCR products using primers (primer set c-4 with three mismatched base at nt 63 961, nt 63 966 and nt 63 971, A to T, C to T and G to T). The oligonucleotide sequences used for the forward primers are as follows: primer set c-1, 5′-GCAGGCACCAAGAACTACTG-3′; primer set c-2, 5′-GCAGGCACC T AGAACTACTG-3′; primer set c-3, 5′-GCAGGCACC T AGAA T TACTG-3′; and primer set c-4, 5′-GCAG T CACC T AGAA T TACTG-3′. The sequences for the backward primers are 5′-TCACCTCCCAGCCTGGCCCA-3′ for ( a ), 5′-AGGGAGATGTTCTCATAAAT-3′ and 5′-CTGTAAGTGGCAGACATTAC-3′ for ( b ). Nucleotide numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Article Snippet: The same results were obtained by the other polymerase systems: LA Taq polymerase (Takara-bio), Tth polymerase (Applied-boisystems); Expand High Fidelity, Expand High FidelityPLUS and Expand Long Template polymerase (Roche-diagnostics); TITANIUM Taq polymerase (Becton-Dickinson-Clontech); and Taq polymerase (Promega).

    Techniques: Polymerase Chain Reaction

    Effect of T.thermophilus RecA protein on PCR. PCR with Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio) for several randomly selected sequences (300–650 bp) in human genomic DNA was carried out in the absence or in the presence of the Tth RecA protein. ( a ) Control, PCR under the standard conditions described in Materials and Methods. ( b ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture). ( c ) Similar to (a), but with ATP (400 μM). ( d ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP (300 μM). ( e ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP-γS (300 μM). The products were electrophoresed and stained with ethidium bromide. Molecular weight markers are indicated on the right-hand side of these panels. The oligonucleotide sequences used for the primers were as follows: 5′-ACAATGGGCTCACTCACCCA-3′ and 5′-CTAAGACCAATGGATAGCTG-3′ for lane 1 (300 bp); 5′-GCTCAGCATGGTGGTGGCAT-3′ and 5′-CCTCATACCTTCCCCCCCAT-3′ for lane 2 (319 bp); 5′-GACTACTCTAGCGACTGTCC-3′ and 5′-GACAGCCACCAGATCCAATC-3′ for lane 3 (360 bp); 5′-AACCTCACAACCTTGGCTGA-3′ and 5′-TTCACAACTTAAGATTTGGC-3′ for lane 4 (400 bp); 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-CAGGGAGCGTGTCCATAGGG-3′ for lane 5 (450 bp); 5′-CTGCTGAAAGAGATGCGGTG-3′ and 5′-AGGAAAACAGCCCAAGGGAC-3′ for lane 6 (469 bp); and 5′-ACTTTGTTCTGAGCCTCACA-3′ and 5′-GTTGCCCAATCGCCCCTCTC-3′ for lane 7 (650 bp).

    Journal: Nucleic Acids Research

    Article Title: Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

    doi: 10.1093/nar/gni111

    Figure Lengend Snippet: Effect of T.thermophilus RecA protein on PCR. PCR with Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio) for several randomly selected sequences (300–650 bp) in human genomic DNA was carried out in the absence or in the presence of the Tth RecA protein. ( a ) Control, PCR under the standard conditions described in Materials and Methods. ( b ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture). ( c ) Similar to (a), but with ATP (400 μM). ( d ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP (300 μM). ( e ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP-γS (300 μM). The products were electrophoresed and stained with ethidium bromide. Molecular weight markers are indicated on the right-hand side of these panels. The oligonucleotide sequences used for the primers were as follows: 5′-ACAATGGGCTCACTCACCCA-3′ and 5′-CTAAGACCAATGGATAGCTG-3′ for lane 1 (300 bp); 5′-GCTCAGCATGGTGGTGGCAT-3′ and 5′-CCTCATACCTTCCCCCCCAT-3′ for lane 2 (319 bp); 5′-GACTACTCTAGCGACTGTCC-3′ and 5′-GACAGCCACCAGATCCAATC-3′ for lane 3 (360 bp); 5′-AACCTCACAACCTTGGCTGA-3′ and 5′-TTCACAACTTAAGATTTGGC-3′ for lane 4 (400 bp); 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-CAGGGAGCGTGTCCATAGGG-3′ for lane 5 (450 bp); 5′-CTGCTGAAAGAGATGCGGTG-3′ and 5′-AGGAAAACAGCCCAAGGGAC-3′ for lane 6 (469 bp); and 5′-ACTTTGTTCTGAGCCTCACA-3′ and 5′-GTTGCCCAATCGCCCCTCTC-3′ for lane 7 (650 bp).

    Article Snippet: The same results were obtained by the other polymerase systems: LA Taq polymerase (Takara-bio), Tth polymerase (Applied-boisystems); Expand High Fidelity, Expand High FidelityPLUS and Expand Long Template polymerase (Roche-diagnostics); TITANIUM Taq polymerase (Becton-Dickinson-Clontech); and Taq polymerase (Promega).

    Techniques: Polymerase Chain Reaction, Staining, Molecular Weight

    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.

    Journal: Journal of Veterinary Science

    Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates

    doi: 10.4142/jvs.2006.7.3.277

    Figure Lengend Snippet: PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.

    Article Snippet: The PCR conditions consisted of 5 µl (50 ng/µl) of DNA and 1 µl each of primer (50 pM) in a 5 µl of 10× reaction buffer with 5 µl of 25 mM MgCl2 , 5 µl of 10 mM dNTP (each 2.5 mM) and 1 µl of 5 U Ex Taq DNA polymerase (TaKaRa, Japan) to a final volume of 50 µl on a thermal cycler (PTC-100; MJ Research, USA).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .

    Journal: Nucleic Acids Research

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification

    doi: 10.1093/nar/gkr1068

    Figure Lengend Snippet: Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .

    Article Snippet: Indeed, our recent study showed that TITANIUM Taq DNA pol exhibited high selectivity and efficiency in a one-way incorporation of modified d Px TP opposite Ds in templates, and is useful for the unnatural base incorporation in a primer region in PCR ( ).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification