talon superflow metal affinity resin Search Results


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  • 99
    TaKaRa talon superflow metal affinity resin
    Talon Superflow Metal Affinity Resin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/talon superflow metal affinity resin/product/TaKaRa
    Average 99 stars, based on 592 article reviews
    Price from $9.99 to $1999.99
    talon superflow metal affinity resin - by Bioz Stars, 2020-01
    99/100 stars
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    80
    TaKaRa talon metal affinity superflow resin
    Talon Metal Affinity Superflow Resin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/talon metal affinity superflow resin/product/TaKaRa
    Average 80 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    talon metal affinity superflow resin - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    88
    Becton Dickinson talon superflow metal affinity resin
    Talon Superflow Metal Affinity Resin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/talon superflow metal affinity resin/product/Becton Dickinson
    Average 88 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    talon superflow metal affinity resin - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    88
    Millipore talon superflow metal affinity resin
    Purification of recombinant hVN from N. benthamiana using a three‐stage extraction protocol. Total leaf protein solubilized in 9 m urea (lane 1) was passed through a column containing Heparin Sepharose 6 Fast Flow resin and bound hVN eluted under high salt (500 m m NaCl) conditions (lane 2). HIS‐tagged hVN was then immobilized in <t>TALON</t> ® <t>Superflow</t> Metal Affinity Resin and eluted using imidazole (lane 3). Plant‐made hVN was further subjected to size exclusion filtration using an Amicon Ultra Centrifugal Filter (Ultracel‐30K) in order to remove low molecular weight protein contaminants and concentrate the product (lane 4). Protein samples representing each major step of the 3‐stage extraction protocol were electrophoresed through a 12% SDS‐polyacrylamide gel and stained with Coomassie Brilliant Blue R‐250. M = prestained SDS‐PAGE standards, low range (Bio‐Rad). Molecular weights marked in kDa.
    Talon Superflow Metal Affinity Resin, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/talon superflow metal affinity resin/product/Millipore
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    talon superflow metal affinity resin - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    78
    Becton Dickinson m imidazole talon superflow metal affinity resin
    Purification of recombinant hVN from N. benthamiana using a three‐stage extraction protocol. Total leaf protein solubilized in 9 m urea (lane 1) was passed through a column containing Heparin Sepharose 6 Fast Flow resin and bound hVN eluted under high salt (500 m m NaCl) conditions (lane 2). HIS‐tagged hVN was then immobilized in <t>TALON</t> ® <t>Superflow</t> Metal Affinity Resin and eluted using imidazole (lane 3). Plant‐made hVN was further subjected to size exclusion filtration using an Amicon Ultra Centrifugal Filter (Ultracel‐30K) in order to remove low molecular weight protein contaminants and concentrate the product (lane 4). Protein samples representing each major step of the 3‐stage extraction protocol were electrophoresed through a 12% SDS‐polyacrylamide gel and stained with Coomassie Brilliant Blue R‐250. M = prestained SDS‐PAGE standards, low range (Bio‐Rad). Molecular weights marked in kDa.
    M Imidazole Talon Superflow Metal Affinity Resin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m imidazole talon superflow metal affinity resin/product/Becton Dickinson
    Average 78 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    m imidazole talon superflow metal affinity resin - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    Image Search Results


    Purification of recombinant hVN from N. benthamiana using a three‐stage extraction protocol. Total leaf protein solubilized in 9 m urea (lane 1) was passed through a column containing Heparin Sepharose 6 Fast Flow resin and bound hVN eluted under high salt (500 m m NaCl) conditions (lane 2). HIS‐tagged hVN was then immobilized in TALON ® Superflow Metal Affinity Resin and eluted using imidazole (lane 3). Plant‐made hVN was further subjected to size exclusion filtration using an Amicon Ultra Centrifugal Filter (Ultracel‐30K) in order to remove low molecular weight protein contaminants and concentrate the product (lane 4). Protein samples representing each major step of the 3‐stage extraction protocol were electrophoresed through a 12% SDS‐polyacrylamide gel and stained with Coomassie Brilliant Blue R‐250. M = prestained SDS‐PAGE standards, low range (Bio‐Rad). Molecular weights marked in kDa.

    Journal: Plant Biotechnology Journal

    Article Title: Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform

    doi: 10.1111/pbi.12779

    Figure Lengend Snippet: Purification of recombinant hVN from N. benthamiana using a three‐stage extraction protocol. Total leaf protein solubilized in 9 m urea (lane 1) was passed through a column containing Heparin Sepharose 6 Fast Flow resin and bound hVN eluted under high salt (500 m m NaCl) conditions (lane 2). HIS‐tagged hVN was then immobilized in TALON ® Superflow Metal Affinity Resin and eluted using imidazole (lane 3). Plant‐made hVN was further subjected to size exclusion filtration using an Amicon Ultra Centrifugal Filter (Ultracel‐30K) in order to remove low molecular weight protein contaminants and concentrate the product (lane 4). Protein samples representing each major step of the 3‐stage extraction protocol were electrophoresed through a 12% SDS‐polyacrylamide gel and stained with Coomassie Brilliant Blue R‐250. M = prestained SDS‐PAGE standards, low range (Bio‐Rad). Molecular weights marked in kDa.

    Article Snippet: A column of TALON® Superflow Metal Affinity Resin (Sigma) was prepared with a packed bed volume equivalent to 1 mL/5 g leaf material and equilibrated with three column volumes of HS Elution buffer.

    Techniques: Purification, Recombinant, Flow Cytometry, Filtration, Molecular Weight, Staining, SDS Page