tagmentation Search Results


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  • 99
    Millipore tagmentation buffer
    Tagmentation Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmentation buffer/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    tagmentation buffer - by Bioz Stars, 2020-08
    99/100 stars
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    93
    Illumina Inc tagmentation
    Current workflow of single-cell technologies to study cortical development. Step1. Biological systems to study brain development. Upper panel shows in vivo mouse embryonic brain and below panel indicates in vitro human brain organoid which is commonly used for the single-cell neurogenesis studies. Step2. Cell isolation methods. Individual cells can be isolated using FACS, Microfluidic ChIP, or Drop-seq approaches. Step3. Library preparation. The common protocols include polyA+ mRNA capture, reverse transcription, cDNA amplification using PCR, and <t>tagmentation.</t> Step4. Sequencing of the library. Step5. Computational analysis. After the preprocessing of sequencing reads, visualization using t-SNE, unsupervised clustering, and correlation analysis with bulk RNA-seq is followed to identify subtypes of cells and characterize their identities.
    Tagmentation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 1019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmentation/product/Illumina Inc
    Average 93 stars, based on 1019 article reviews
    Price from $9.99 to $1999.99
    tagmentation - by Bioz Stars, 2020-08
    93/100 stars
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    91
    Illumina Inc tagment buffer
    Current workflow of single-cell technologies to study cortical development. Step1. Biological systems to study brain development. Upper panel shows in vivo mouse embryonic brain and below panel indicates in vitro human brain organoid which is commonly used for the single-cell neurogenesis studies. Step2. Cell isolation methods. Individual cells can be isolated using FACS, Microfluidic ChIP, or Drop-seq approaches. Step3. Library preparation. The common protocols include polyA+ mRNA capture, reverse transcription, cDNA amplification using PCR, and <t>tagmentation.</t> Step4. Sequencing of the library. Step5. Computational analysis. After the preprocessing of sequencing reads, visualization using t-SNE, unsupervised clustering, and correlation analysis with bulk RNA-seq is followed to identify subtypes of cells and characterize their identities.
    Tagment Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagment buffer/product/Illumina Inc
    Average 91 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    tagment buffer - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    Illumina Inc tagmentation enzyme
    Current workflow of single-cell technologies to study cortical development. Step1. Biological systems to study brain development. Upper panel shows in vivo mouse embryonic brain and below panel indicates in vitro human brain organoid which is commonly used for the single-cell neurogenesis studies. Step2. Cell isolation methods. Individual cells can be isolated using FACS, Microfluidic ChIP, or Drop-seq approaches. Step3. Library preparation. The common protocols include polyA+ mRNA capture, reverse transcription, cDNA amplification using PCR, and <t>tagmentation.</t> Step4. Sequencing of the library. Step5. Computational analysis. After the preprocessing of sequencing reads, visualization using t-SNE, unsupervised clustering, and correlation analysis with bulk RNA-seq is followed to identify subtypes of cells and characterize their identities.
    Tagmentation Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmentation enzyme/product/Illumina Inc
    Average 92 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    tagmentation enzyme - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    88
    Illumina Inc nextera tagmentation
    Current workflow of single-cell technologies to study cortical development. Step1. Biological systems to study brain development. Upper panel shows in vivo mouse embryonic brain and below panel indicates in vitro human brain organoid which is commonly used for the single-cell neurogenesis studies. Step2. Cell isolation methods. Individual cells can be isolated using FACS, Microfluidic ChIP, or Drop-seq approaches. Step3. Library preparation. The common protocols include polyA+ mRNA capture, reverse transcription, cDNA amplification using PCR, and <t>tagmentation.</t> Step4. Sequencing of the library. Step5. Computational analysis. After the preprocessing of sequencing reads, visualization using t-SNE, unsupervised clustering, and correlation analysis with bulk RNA-seq is followed to identify subtypes of cells and characterize their identities.
    Nextera Tagmentation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera tagmentation/product/Illumina Inc
    Average 88 stars, based on 119 article reviews
    Price from $9.99 to $1999.99
    nextera tagmentation - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    89
    Illumina Inc tagmentation buffer
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Tagmentation Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmentation buffer/product/Illumina Inc
    Average 89 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    tagmentation buffer - by Bioz Stars, 2020-08
    89/100 stars
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    91
    Illumina Inc chip tagmentation
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Chip Tagmentation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip tagmentation/product/Illumina Inc
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    chip tagmentation - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    85
    Epicentre Biotechnologies nextera tagmentation system
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Nextera Tagmentation System, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera tagmentation system/product/Epicentre Biotechnologies
    Average 85 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    nextera tagmentation system - by Bioz Stars, 2020-08
    85/100 stars
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    90
    Illumina Inc nexteraxt tagmentation kit
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Nexteraxt Tagmentation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nexteraxt tagmentation kit/product/Illumina Inc
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    nexteraxt tagmentation kit - by Bioz Stars, 2020-08
    90/100 stars
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    91
    ATUM tagmented dna
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Tagmented Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmented dna/product/ATUM
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    tagmented dna - by Bioz Stars, 2020-08
    91/100 stars
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    91
    Illumina Inc neutralize tagment buffer
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Neutralize Tagment Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutralize tagment buffer/product/Illumina Inc
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    neutralize tagment buffer - by Bioz Stars, 2020-08
    91/100 stars
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    92
    Epicentre Biotechnologies nextera tagmentation kit
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Nextera Tagmentation Kit, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera tagmentation kit/product/Epicentre Biotechnologies
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    nextera tagmentation kit - by Bioz Stars, 2020-08
    92/100 stars
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    88
    Illumina Inc nextera tagmentation protocol
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Nextera Tagmentation Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera tagmentation protocol/product/Illumina Inc
    Average 88 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    nextera tagmentation protocol - by Bioz Stars, 2020-08
    88/100 stars
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    86
    Illumina Inc nextera tagmentation chemistry
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Nextera Tagmentation Chemistry, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera tagmentation chemistry/product/Illumina Inc
    Average 86 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    nextera tagmentation chemistry - by Bioz Stars, 2020-08
    86/100 stars
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    91
    Illumina Inc nextera tagmentation reaction
    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct <t>tagmentation</t> of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated
    Nextera Tagmentation Reaction, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera tagmentation reaction/product/Illumina Inc
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nextera tagmentation reaction - by Bioz Stars, 2020-08
    91/100 stars
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    91
    New England Biolabs tagmentation
    Tn5 transposome has direct <t>tagmentation</t> activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.
    Tagmentation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmentation/product/New England Biolabs
    Average 91 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    tagmentation - by Bioz Stars, 2020-08
    91/100 stars
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    90
    fluidigm tagmentation
    Tn5 transposome has direct <t>tagmentation</t> activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.
    Tagmentation, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmentation/product/fluidigm
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    tagmentation - by Bioz Stars, 2020-08
    90/100 stars
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    90
    Illumina Inc tagmentation dna buffer
    Tn5 transposome has direct <t>tagmentation</t> activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.
    Tagmentation Dna Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tagmentation dna buffer/product/Illumina Inc
    Average 90 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    tagmentation dna buffer - by Bioz Stars, 2020-08
    90/100 stars
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    93
    Millipore tagmentation mix
    Tn5 transposome has direct <t>tagmentation</t> activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.
    Tagmentation Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc amplicon tagment mix
    Tn5 transposome has direct <t>tagmentation</t> activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.
    Amplicon Tagment Mix, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc mate pair tagmentation enzyme
    Tn5 transposome has direct <t>tagmentation</t> activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.
    Mate Pair Tagmentation Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Current workflow of single-cell technologies to study cortical development. Step1. Biological systems to study brain development. Upper panel shows in vivo mouse embryonic brain and below panel indicates in vitro human brain organoid which is commonly used for the single-cell neurogenesis studies. Step2. Cell isolation methods. Individual cells can be isolated using FACS, Microfluidic ChIP, or Drop-seq approaches. Step3. Library preparation. The common protocols include polyA+ mRNA capture, reverse transcription, cDNA amplification using PCR, and tagmentation. Step4. Sequencing of the library. Step5. Computational analysis. After the preprocessing of sequencing reads, visualization using t-SNE, unsupervised clustering, and correlation analysis with bulk RNA-seq is followed to identify subtypes of cells and characterize their identities.

    Journal: Frontiers in Neuroscience

    Article Title: Exploring the Complexity of Cortical Development Using Single-Cell Transcriptomics

    doi: 10.3389/fnins.2018.00031

    Figure Lengend Snippet: Current workflow of single-cell technologies to study cortical development. Step1. Biological systems to study brain development. Upper panel shows in vivo mouse embryonic brain and below panel indicates in vitro human brain organoid which is commonly used for the single-cell neurogenesis studies. Step2. Cell isolation methods. Individual cells can be isolated using FACS, Microfluidic ChIP, or Drop-seq approaches. Step3. Library preparation. The common protocols include polyA+ mRNA capture, reverse transcription, cDNA amplification using PCR, and tagmentation. Step4. Sequencing of the library. Step5. Computational analysis. After the preprocessing of sequencing reads, visualization using t-SNE, unsupervised clustering, and correlation analysis with bulk RNA-seq is followed to identify subtypes of cells and characterize their identities.

    Article Snippet: In case of Smart-seq, commercially available Smart-seq kit (Clontech) is used to generate full-length double-stranded cDNA which is converted into sequencing libraries by tagmentation (Nextera, Illumina) (Ziegenhain et al., ).

    Techniques: In Vivo, In Vitro, Cell Isolation, Isolation, FACS, Chromatin Immunoprecipitation, Amplification, Polymerase Chain Reaction, Sequencing, RNA Sequencing Assay

    High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct tagmentation of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated

    Journal: BMC Genomics

    Article Title: High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers

    doi: 10.1186/s12864-018-5299-0

    Figure Lengend Snippet: High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1 : Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct tagmentation of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated

    Article Snippet: Thirty microliters of tagmentation buffer and 1 μl transposase (Nextera, Illumina) was added, and samples were incubated at 37 °C for 10 min. 22.5 μl of the transposition reaction were combined with 15 μl of PCR master mix and 2.5 μl of primer mix (Nextera, Illumina).

    Techniques: High Throughput Screening Assay, Amplification, Binding Assay, FACS, Sonication, Chromatin Immunoprecipitation, DNA Purification, Generated, Sequencing

    Tn5 transposome has direct tagmentation activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.

    Journal: bioRxiv

    Article Title: Transposase assisted tagmentation of RNA/DNA hybrid duplexes

    doi: 10.1101/2020.01.29.926105

    Figure Lengend Snippet: Tn5 transposome has direct tagmentation activity on RNA/DNA hybrid duplexes. (a) Crystal structure of a single subunit of E. coli Tn5 Transposase (PDB code 1MM8) complexed with ME DNA duplex, and zoom-in views of the conserved catalytic core of Tn5 transposase, HIV-1 integrase (PDB code 1BIU), and E. coli RNase HI (PDB code 1G15), all of which are from the retroviral integrase superfamily. Active-site residues are shown as sticks, and the Mn 2+ and Mg 2+ ions are shown as deep blue and magenta spheres. (b) Schematic of Tn5-assisted tagmentation of RNA/DNA hybrids. (c) Gel pictures (left) and peak pictures (right) represent size distributions of RNA/DNA hybrid fragments after incubation without Tn5 transposome, with Tn5 transposome, and with inactivated Tn5 transposome. The blue and orange patches denote small and large fragments, respectively. (d) Schematic of the product of in vitro tagmentation reaction of the canonical dsDNA substrate. (e) Workflow of conversion of tagged RNA/DNA hybrids into amplifiable DNA sequences. (f) qPCR amplification curve of tagmentation products of samples with Tn5 treatment, with inactivated Tn5 treatment, or without Tn5 treatment. Average Ct values of two technical replicates are 18.06, 26.25 and 26.41, respectively.

    Article Snippet: Strand extension reaction was performed by directly adding 0.32 U/μl Bst 3.0 DNA Polymerase and 1X NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB) to tagmentation products and incubating at 72°C for 15 min, followed by Bst 3.0 DNA Polymerase inactivation at 80°C for 20 min. Next, 0.2 μM indexed primers were added to perform enrichment PCR as follows: 30 sec at 98°C, and then n cycles of 10 sec at 98°C, 75 sec at 65°C, followed by the last 10min extension at 65°C.

    Techniques: Activity Assay, Incubation, In Vitro, Real-time Polymerase Chain Reaction, Amplification

    Tagmentation activity of Tn5 transposome on RNA/DNA hybrids. (a) Denaturing (8 M urea) polyacrylamide gel analysis of reverse transcription products of an in vitro transcribed mRNA (IRF9). Lane 1: ssRNA marker. Lane 2: in vitro transcribed mRNA (IRF9). Lane 3 4: reverse transcription products of an in vitro transcribed mRNA (IRF9). Lane 5: reverse transcription product treated with DNase I. Lane 6: reverse transcription product treated with RNase H. ssRNA and ssDNA is marked with a red asterisk and a blue pound sign, respectively. (b) Gel picture showing size distribution of RNA/DNA hybrids products of 50 μl reaction systems without Tn5 transposome, and with 5 μl, 10 μl, and 15 μl Tn5 transposome, respectively. The blue and orange patches denote small and large fragments, respectively. (c) qPCR amplification curve of tagmentation products without Tn5 treatment or with Tn5 treatment in three different buffers (see methods). Average Ct values are 26.41, 18.39, 18.33 and 18.34, respectively. (d) Sanger sequencing chromatograms of PCR products following RNA/DNA hybrid tagmentation and strand extension. Adaptor A and B sequences are highlighted with blue background color and insert sequences are highlighted with yellow background.

    Journal: bioRxiv

    Article Title: Transposase assisted tagmentation of RNA/DNA hybrid duplexes

    doi: 10.1101/2020.01.29.926105

    Figure Lengend Snippet: Tagmentation activity of Tn5 transposome on RNA/DNA hybrids. (a) Denaturing (8 M urea) polyacrylamide gel analysis of reverse transcription products of an in vitro transcribed mRNA (IRF9). Lane 1: ssRNA marker. Lane 2: in vitro transcribed mRNA (IRF9). Lane 3 4: reverse transcription products of an in vitro transcribed mRNA (IRF9). Lane 5: reverse transcription product treated with DNase I. Lane 6: reverse transcription product treated with RNase H. ssRNA and ssDNA is marked with a red asterisk and a blue pound sign, respectively. (b) Gel picture showing size distribution of RNA/DNA hybrids products of 50 μl reaction systems without Tn5 transposome, and with 5 μl, 10 μl, and 15 μl Tn5 transposome, respectively. The blue and orange patches denote small and large fragments, respectively. (c) qPCR amplification curve of tagmentation products without Tn5 treatment or with Tn5 treatment in three different buffers (see methods). Average Ct values are 26.41, 18.39, 18.33 and 18.34, respectively. (d) Sanger sequencing chromatograms of PCR products following RNA/DNA hybrid tagmentation and strand extension. Adaptor A and B sequences are highlighted with blue background color and insert sequences are highlighted with yellow background.

    Article Snippet: Strand extension reaction was performed by directly adding 0.32 U/μl Bst 3.0 DNA Polymerase and 1X NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB) to tagmentation products and incubating at 72°C for 15 min, followed by Bst 3.0 DNA Polymerase inactivation at 80°C for 20 min. Next, 0.2 μM indexed primers were added to perform enrichment PCR as follows: 30 sec at 98°C, and then n cycles of 10 sec at 98°C, 75 sec at 65°C, followed by the last 10min extension at 65°C.

    Techniques: Activity Assay, In Vitro, Marker, Real-time Polymerase Chain Reaction, Amplification, Sequencing, Polymerase Chain Reaction