tag-pcr Search Results


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  • 99
    Thermo Fisher pcr tag master mix
    Pcr Tag Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co tag pcr mastermix
    Tag Pcr Mastermix, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche tag pcr mix
    Tag Pcr Mix, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc tag polymerase chain reactions pcr
    Tag Polymerase Chain Reactions Pcr, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen tag pcr core kit
    Tag Pcr Core Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Luminex id tag polymerase chain reaction
    Id Tag Polymerase Chain Reaction, supplied by Luminex, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa ex tag pcr kit
    Ex Tag Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Advanced Biotechnologies Inc gold tag pcr enzyme
    Gold Tag Pcr Enzyme, supplied by Advanced Biotechnologies Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad tag pcr master mix kit
    Tag Pcr Master Mix Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega polymerase chain reaction pcr amplified halo tag amplified halo tag template pfn22k
    Polymerase Chain Reaction Pcr Amplified Halo Tag Amplified Halo Tag Template Pfn22k, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc its2 illumina tag pcr
    Its2 Illumina Tag Pcr, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen tag pcr master mix kit
    Tag Pcr Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa green tag pcr mix solution
    Green Tag Pcr Mix Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr premix tag
    Pcr Premix Tag, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioneer Corporation accupower tagpcr premix
    Accupower Tagpcr Premix, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa long range pcr tag
    Long Range Pcr Tag, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pcr amplified halo tag
    Pcr Amplified Halo Tag, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare tag ready to go pcr beads
    Tag Ready To Go Pcr Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies cjnc1 cjnc4 cjnc3 pcr tag primers
    Inactivation and overexpression of <t>CjNC1</t> and <t>CjNC4</t> does not affect flagella-related phenotypes in C . jejuni . (a) The successful inactivation (indicated by Δ) and overexpression (indicated by ov ) of CjNC4 is demonstrated using Northern hybridisation. The hybridisation of <t>CjNC3</t> is shown as loading control. The first two lanes of the Northern hybridisation are also shown in Fig 1C , left panel. (b) Inactivation or overexpression of CjNC1 and CjNC4 does not change cell morphology or production of flagella, as shown by light microscopy (×10,000), with cells and flagella stained by modified Ryu staining [ 44 , 45 ]. Cutouts show representative cells, the Δ flaAB non-motile mutant is included for comparison. (c) Inactivation or overexpression of CjNC1 or CjNC4 does not significantly affect motility on swarm plates, as measured by the diameter of the swarming zone on motility agar plates. Results shown are the average of at least three independent experiments, error bars indicate standard error of the mean. The asterisk indicates P
    Cjnc1 Cjnc4 Cjnc3 Pcr Tag Primers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr premix ex tag quantitative pcr kit
    Inactivation and overexpression of <t>CjNC1</t> and <t>CjNC4</t> does not affect flagella-related phenotypes in C . jejuni . (a) The successful inactivation (indicated by Δ) and overexpression (indicated by ov ) of CjNC4 is demonstrated using Northern hybridisation. The hybridisation of <t>CjNC3</t> is shown as loading control. The first two lanes of the Northern hybridisation are also shown in Fig 1C , left panel. (b) Inactivation or overexpression of CjNC1 and CjNC4 does not change cell morphology or production of flagella, as shown by light microscopy (×10,000), with cells and flagella stained by modified Ryu staining [ 44 , 45 ]. Cutouts show representative cells, the Δ flaAB non-motile mutant is included for comparison. (c) Inactivation or overexpression of CjNC1 or CjNC4 does not significantly affect motility on swarm plates, as measured by the diameter of the swarming zone on motility agar plates. Results shown are the average of at least three independent experiments, error bars indicate standard error of the mean. The asterisk indicates P
    Sybr Premix Ex Tag Quantitative Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Luminex rt pcr id tag rvp
    Inactivation and overexpression of <t>CjNC1</t> and <t>CjNC4</t> does not affect flagella-related phenotypes in C . jejuni . (a) The successful inactivation (indicated by Δ) and overexpression (indicated by ov ) of CjNC4 is demonstrated using Northern hybridisation. The hybridisation of <t>CjNC3</t> is shown as loading control. The first two lanes of the Northern hybridisation are also shown in Fig 1C , left panel. (b) Inactivation or overexpression of CjNC1 and CjNC4 does not change cell morphology or production of flagella, as shown by light microscopy (×10,000), with cells and flagella stained by modified Ryu staining [ 44 , 45 ]. Cutouts show representative cells, the Δ flaAB non-motile mutant is included for comparison. (c) Inactivation or overexpression of CjNC1 or CjNC4 does not significantly affect motility on swarm plates, as measured by the diameter of the swarming zone on motility agar plates. Results shown are the average of at least three independent experiments, error bars indicate standard error of the mean. The asterisk indicates P
    Rt Pcr Id Tag Rvp, supplied by Luminex, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inactivation and overexpression of CjNC1 and CjNC4 does not affect flagella-related phenotypes in C . jejuni . (a) The successful inactivation (indicated by Δ) and overexpression (indicated by ov ) of CjNC4 is demonstrated using Northern hybridisation. The hybridisation of CjNC3 is shown as loading control. The first two lanes of the Northern hybridisation are also shown in Fig 1C , left panel. (b) Inactivation or overexpression of CjNC1 and CjNC4 does not change cell morphology or production of flagella, as shown by light microscopy (×10,000), with cells and flagella stained by modified Ryu staining [ 44 , 45 ]. Cutouts show representative cells, the Δ flaAB non-motile mutant is included for comparison. (c) Inactivation or overexpression of CjNC1 or CjNC4 does not significantly affect motility on swarm plates, as measured by the diameter of the swarming zone on motility agar plates. Results shown are the average of at least three independent experiments, error bars indicate standard error of the mean. The asterisk indicates P

    Journal: PLoS ONE

    Article Title: Conservation of σ28-Dependent Non-Coding RNA Paralogs and Predicted σ54-Dependent Targets in Thermophilic Campylobacter Species

    doi: 10.1371/journal.pone.0141627

    Figure Lengend Snippet: Inactivation and overexpression of CjNC1 and CjNC4 does not affect flagella-related phenotypes in C . jejuni . (a) The successful inactivation (indicated by Δ) and overexpression (indicated by ov ) of CjNC4 is demonstrated using Northern hybridisation. The hybridisation of CjNC3 is shown as loading control. The first two lanes of the Northern hybridisation are also shown in Fig 1C , left panel. (b) Inactivation or overexpression of CjNC1 and CjNC4 does not change cell morphology or production of flagella, as shown by light microscopy (×10,000), with cells and flagella stained by modified Ryu staining [ 44 , 45 ]. Cutouts show representative cells, the Δ flaAB non-motile mutant is included for comparison. (c) Inactivation or overexpression of CjNC1 or CjNC4 does not significantly affect motility on swarm plates, as measured by the diameter of the swarming zone on motility agar plates. Results shown are the average of at least three independent experiments, error bars indicate standard error of the mean. The asterisk indicates P

    Article Snippet: The cDNA was produced with the CjNC1/CjNC4/CjNC3 PCR-tag primers ( ) and Affinity Script (Agilent), followed by a PCR reaction with CjNC1, CjNC3 and CjNC4-specific forward primers and the tag-specific reverse primer.

    Techniques: Over Expression, Northern Blot, Hybridization, Light Microscopy, Staining, Modification, Mutagenesis

    The CjNC1 and CjNC4 non-coding RNAs are paralogs, expressed from σ 28 -dependent promoters. (a) Schematic representation of the genomic position, transcriptional orientation and surrounding genes of the CjNC1 and CjNC4 ncRNAs in C . jejuni NCTC 11168. Arrows indicate the position of transcription start sites of loci as mapped by dRNA-seq [ 24 ]. (b) Alignment and structure prediction of the C . jejuni NCTC 11168 CjNC1 and CjNC4 ncRNAs. The canonical -10 sequence of the σ 28 -dependent promoter is indicated in red, the transcription start site (TSS) is underlined. The blue residues are the conserved region predicted to interact with target 5' UTRs, and the green residues highlight the complementary nucleotides predicted to form the stem-loop structure which could function as transcriptional terminator. Asterisks indicate conserved nucleotides. (c) Transcription of CjNC1 and CjNC4 is dependent on σ 28 , as shown by comparing transcript levels in wildtype and fliA mutant of C . jejuni NCTC 11168, by Northern hybridisation (left) for CjNC4, and by RT-PCR for CjNC1 and CjNC4 (right), while transcription of the σ 28 -dependent CjNC3 ncRNA is not affected by the inactivation of fliA . The RT-PCR utilised a tag-based primer attached by the reverse transcription process (see Methods ) and hence cannot amplify genomic DNA, which is includes as control (gDNA).

    Journal: PLoS ONE

    Article Title: Conservation of σ28-Dependent Non-Coding RNA Paralogs and Predicted σ54-Dependent Targets in Thermophilic Campylobacter Species

    doi: 10.1371/journal.pone.0141627

    Figure Lengend Snippet: The CjNC1 and CjNC4 non-coding RNAs are paralogs, expressed from σ 28 -dependent promoters. (a) Schematic representation of the genomic position, transcriptional orientation and surrounding genes of the CjNC1 and CjNC4 ncRNAs in C . jejuni NCTC 11168. Arrows indicate the position of transcription start sites of loci as mapped by dRNA-seq [ 24 ]. (b) Alignment and structure prediction of the C . jejuni NCTC 11168 CjNC1 and CjNC4 ncRNAs. The canonical -10 sequence of the σ 28 -dependent promoter is indicated in red, the transcription start site (TSS) is underlined. The blue residues are the conserved region predicted to interact with target 5' UTRs, and the green residues highlight the complementary nucleotides predicted to form the stem-loop structure which could function as transcriptional terminator. Asterisks indicate conserved nucleotides. (c) Transcription of CjNC1 and CjNC4 is dependent on σ 28 , as shown by comparing transcript levels in wildtype and fliA mutant of C . jejuni NCTC 11168, by Northern hybridisation (left) for CjNC4, and by RT-PCR for CjNC1 and CjNC4 (right), while transcription of the σ 28 -dependent CjNC3 ncRNA is not affected by the inactivation of fliA . The RT-PCR utilised a tag-based primer attached by the reverse transcription process (see Methods ) and hence cannot amplify genomic DNA, which is includes as control (gDNA). "Neg" represents the negative PCR-control. The addition of the tag adds 29 nt to the size of RT-PCR products.

    Article Snippet: The cDNA was produced with the CjNC1/CjNC4/CjNC3 PCR-tag primers ( ) and Affinity Script (Agilent), followed by a PCR reaction with CjNC1, CjNC3 and CjNC4-specific forward primers and the tag-specific reverse primer.

    Techniques: Sequencing, Mutagenesis, Northern Blot, Hybridization, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction