Article Title: Simple, Functional, Inexpensive Cell Extract for in vitro Prototyping of Proteins with Disulfide Bonds
Figure Lengend Snippet: Experiments to compare the yield of T7 SHuffle ® , KGK10, BL21 DE3 Star, and the PURE frex 2.1 kit. KGK10 #1 is carefully fermented KGK10 extract that was provided by the Swartz lab. KGK10 #2 is grown in-house using a shake flask. (A) Gluc signal comparisons at a gain of 100 without PURE frex data (n=3). (B) Gluc signal comparisons with an instrument gain of 80 so PURE frex data does not saturate the detector. (C) Expression of sfGFP to compare yield of proteins without disulfide bonds over 16 hr (n=3). (D) Is a ratio of oxidation potential/productivity using a YFP-mCherry fusion where a S-S bond has been introduced into a YFP variant (n=3).
Article Snippet: T7 SHuffle® The supplement recipe used for the designed experiments and initial tests is a modified version of the PANOx-SP system that is improved to form disulfide bonds ( ).
Techniques: Expressing, Variant Assay