t7 rna polymerase Promega Search Results


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  • 95
    New England Biolabs t7 rna polymerase
    T7 Rna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3044 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 3044 article reviews
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    90
    Thermo Fisher t7 rna polymerase
    T7 Rna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 9704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 9704 article reviews
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    Promega t7 rna polymerase
    Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using <t>T7</t> RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region
    T7 Rna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 8589 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa rna polymerase
    Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using <t>T7</t> RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region
    Rna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs t7 rna polymerases
    Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using <t>T7</t> RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region
    T7 Rna Polymerases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega ribomax t7 rna polymerase kit
    Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using <t>T7</t> RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region
    Ribomax T7 Rna Polymerase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Promega in vitro translation employing t7 rna polymerase
    Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using <t>T7</t> RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region
    In Vitro Translation Employing T7 Rna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rnas polymerases
    Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using <t>T7</t> RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region
    T7 Rnas Polymerases, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase expression vector pgem3z f
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Expression Vector Pgem3z F, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase coupled wheat germ extract system
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Coupled Wheat Germ Extract System, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 9 article reviews
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    82
    Promega t7 rna polymerase reticulocyte lysate coupled transcription translation system
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Reticulocyte Lysate Coupled Transcription Translation System, supplied by Promega, used in various techniques. Bioz Stars score: 82/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t3 t7 rna polymerase dependent in vitro transcription reaction
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T3 T7 Rna Polymerase Dependent In Vitro Transcription Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase coupled tnt kit
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Coupled Tnt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase coupled transcription rabbit reticulocyte translation system
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Coupled Transcription Rabbit Reticulocyte Translation System, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase coupled tnt quick kit
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Coupled Tnt Quick Kit, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega riboprobe ii core system t7 rna polymerase kit
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    Riboprobe Ii Core System T7 Rna Polymerase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 rna polymerase tnt coupled reticulocyte lysate system
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Tnt Coupled Reticulocyte Lysate System, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 7 article reviews
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    78
    Promega t7 rna polymerase rabbit reticulocyte lysate coupled system
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Rabbit Reticulocyte Lysate Coupled System, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t7 phage rna polymerases
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Phage Rna Polymerases, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t3 t7 phage rna polymerases
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T3 T7 Phage Rna Polymerases, supplied by Promega, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega t7 rna polymerase based rabbit reticulocyte lysate coupled transcription translation kit
    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
    T7 Rna Polymerase Based Rabbit Reticulocyte Lysate Coupled Transcription Translation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 rna polymerase based rabbit reticulocyte lysate coupled transcription translation kit/product/Promega
    Average 79 stars, based on 9 article reviews
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    Promega t7 rna polymerase rabbit reticulocyte lysate in vitro transcription translation system
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    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
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    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
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    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid <t>pGEM3z/f±</t> used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a <t>T7</t> RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.
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    The protection of FtsZ proteins from trypsin digestion suggests that these proteins are localized to the chloroplast stroma following import in vitro. A to G, Large-scale import reactions using either radiolabeled AtFtsZ1-1 (A), AtFtsZ2-1 (B), AtFtsZ2-2 (C), or the control proteins, SS (D), <t>tp110-110N,</t> truncated Tic110 (E), HPLS (F), and Tic22 (G), were incubated for 30 min at room temperature. Each import reaction was then divided into five equal fractions. H, Likewise, a large-scale binding reaction with pSS was performed and divided into five equal fractions. Individual import and binding reactions were incubated either without (−) or with 100, 250, or 500 μg trypsin mL −1 in the absence (−) or presence (+) of Triton X-100. Protease digestion reactions were incubated on ice for 30 min and then quenched. Intact chloroplasts recovered by sedimentation were solubilized in sample buffer, separated by SDS-PAGE, and analyzed by fluorography. TP represents 10% of radiolabeled translation product added to the reaction; arrows indicate precursor protein (p) and mature protein (m).
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    The protection of FtsZ proteins from trypsin digestion suggests that these proteins are localized to the chloroplast stroma following import in vitro. A to G, Large-scale import reactions using either radiolabeled AtFtsZ1-1 (A), AtFtsZ2-1 (B), AtFtsZ2-2 (C), or the control proteins, SS (D), <t>tp110-110N,</t> truncated Tic110 (E), HPLS (F), and Tic22 (G), were incubated for 30 min at room temperature. Each import reaction was then divided into five equal fractions. H, Likewise, a large-scale binding reaction with pSS was performed and divided into five equal fractions. Individual import and binding reactions were incubated either without (−) or with 100, 250, or 500 μg trypsin mL −1 in the absence (−) or presence (+) of Triton X-100. Protease digestion reactions were incubated on ice for 30 min and then quenched. Intact chloroplasts recovered by sedimentation were solubilized in sample buffer, separated by SDS-PAGE, and analyzed by fluorography. TP represents 10% of radiolabeled translation product added to the reaction; arrows indicate precursor protein (p) and mature protein (m).
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    The protection of FtsZ proteins from trypsin digestion suggests that these proteins are localized to the chloroplast stroma following import in vitro. A to G, Large-scale import reactions using either radiolabeled AtFtsZ1-1 (A), AtFtsZ2-1 (B), AtFtsZ2-2 (C), or the control proteins, SS (D), <t>tp110-110N,</t> truncated Tic110 (E), HPLS (F), and Tic22 (G), were incubated for 30 min at room temperature. Each import reaction was then divided into five equal fractions. H, Likewise, a large-scale binding reaction with pSS was performed and divided into five equal fractions. Individual import and binding reactions were incubated either without (−) or with 100, 250, or 500 μg trypsin mL −1 in the absence (−) or presence (+) of Triton X-100. Protease digestion reactions were incubated on ice for 30 min and then quenched. Intact chloroplasts recovered by sedimentation were solubilized in sample buffer, separated by SDS-PAGE, and analyzed by fluorography. TP represents 10% of radiolabeled translation product added to the reaction; arrows indicate precursor protein (p) and mature protein (m).
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    The protection of FtsZ proteins from trypsin digestion suggests that these proteins are localized to the chloroplast stroma following import in vitro. A to G, Large-scale import reactions using either radiolabeled AtFtsZ1-1 (A), AtFtsZ2-1 (B), AtFtsZ2-2 (C), or the control proteins, SS (D), <t>tp110-110N,</t> truncated Tic110 (E), HPLS (F), and Tic22 (G), were incubated for 30 min at room temperature. Each import reaction was then divided into five equal fractions. H, Likewise, a large-scale binding reaction with pSS was performed and divided into five equal fractions. Individual import and binding reactions were incubated either without (−) or with 100, 250, or 500 μg trypsin mL −1 in the absence (−) or presence (+) of Triton X-100. Protease digestion reactions were incubated on ice for 30 min and then quenched. Intact chloroplasts recovered by sedimentation were solubilized in sample buffer, separated by SDS-PAGE, and analyzed by fluorography. TP represents 10% of radiolabeled translation product added to the reaction; arrows indicate precursor protein (p) and mature protein (m).
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    The protection of FtsZ proteins from trypsin digestion suggests that these proteins are localized to the chloroplast stroma following import in vitro. A to G, Large-scale import reactions using either radiolabeled AtFtsZ1-1 (A), AtFtsZ2-1 (B), AtFtsZ2-2 (C), or the control proteins, SS (D), <t>tp110-110N,</t> truncated Tic110 (E), HPLS (F), and Tic22 (G), were incubated for 30 min at room temperature. Each import reaction was then divided into five equal fractions. H, Likewise, a large-scale binding reaction with pSS was performed and divided into five equal fractions. Individual import and binding reactions were incubated either without (−) or with 100, 250, or 500 μg trypsin mL −1 in the absence (−) or presence (+) of Triton X-100. Protease digestion reactions were incubated on ice for 30 min and then quenched. Intact chloroplasts recovered by sedimentation were solubilized in sample buffer, separated by SDS-PAGE, and analyzed by fluorography. TP represents 10% of radiolabeled translation product added to the reaction; arrows indicate precursor protein (p) and mature protein (m).
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    Image Search Results


    Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using T7 RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region

    Journal: Journal of Virology

    Article Title: Molecular Determinants of Infectious Pancreatic Necrosis Virus Virulence and Cell Culture Adaptation

    doi: 10.1128/JVI.79.16.10289-10299.2005

    Figure Lengend Snippet: Schematic presentation of IPNV cDNA constructs for the generation of plus-sense RNA transcripts using T7 RNA polymerase. A map of the IPNV genome segments A and B, with its coding capacity, is shown at the top. The open boxes depict the coding region

    Article Snippet: The integrity of the full-length constructs was tested by an in vitro transcription-translation-coupled reticulocyte lysate system using T7 RNA polymerase (Promega Corp.).

    Techniques: Construct

    Deamination activity of WT and mutant AID proteins on a transcribed linear dsDNA. Top , dsDNA template with an incorporated T7 promoter for transcription by T7 RNA polymerase. A single target C residue located on the nontranscribed strands is indicated

    Journal:

    Article Title: Impact of Phosphorylation and Phosphorylation-null Mutants on the Activity and Deamination Specificity of Activation-induced Cytidine Deaminase *Impact of Phosphorylation and Phosphorylation-null Mutants on the Activity and Deamination Specificity of Activation-induced Cytidine Deaminase * S⃞

    doi: 10.1074/jbc.M802121200

    Figure Lengend Snippet: Deamination activity of WT and mutant AID proteins on a transcribed linear dsDNA. Top , dsDNA template with an incorporated T7 promoter for transcription by T7 RNA polymerase. A single target C residue located on the nontranscribed strands is indicated

    Article Snippet: T7 RNA polymerase was purchased from Promega, and ultrapure NTP was purchased from Amersham Biosciences.

    Techniques: Activity Assay, Mutagenesis

    Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid pGEM3z/f± used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a T7 RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.

    Journal: Journal of Virology

    Article Title: The Reovirus S4 Gene 3? Nontranslated Region Contains a Translational Operator Sequence †

    doi: 10.1128/JVI.75.14.6517-6526.2001

    Figure Lengend Snippet: Diagram of templates used for transcription of full-length and deletion-mutant s4 mRNAs. (A) The relevant region of plasmid pGEM3z/f± used for cloning a full-length cDNA corresponding to the S4 gene of reovirus strain T3D. Indicated are a T7 RNA polymerase promoter (T7), which was cloned adjacent to the 5′-terminal guanosine of the S4 gene cDNA, the ς3 open reading frame (ORF), and a Pst I site, which was engineered adjacent to the 3′ terminus of the S4 gene cDNA. Digestion of plasmid constructs with Pst I followed by incubation with T4 DNA polymerase resulted in templates for transcription that initiate with the 5′ guanosine and terminate with the 3′ cytosine of the S4 gene. Capped s4 mRNAs and s4 3′Δ mRNAs were transcribed in vitro and used in translation studies. Nucleotide lengths are indicated on the right. The s4 3′Δ mRNA terminates with UAAC , corresponding to the stop codon of the transcript and the 3′-terminal cytosine of the full-length S4 gene. (B) Schematic of S4 gene 3′NTR deletion constructs generated from cDNA clones by PCR mutagenesis. The viral sequences deleted in each of the mutant S4 gene cDNAs are indicated on the left; the length of each mRNA transcript is indicated on the right.

    Article Snippet: The S4 gene cDNA was ligated into plasmid pCR2.1 (Invitrogen, Carlsbad, Calif.) and then transferred to T7 RNA polymerase expression vector pGEM3z/f± (Promega) following digestion with Eco RI and Pst I (New England Biolabs, Beverly, Mass.).

    Techniques: Mutagenesis, Plasmid Preparation, Clone Assay, Construct, Incubation, In Vitro, Generated, Polymerase Chain Reaction

    The protection of FtsZ proteins from trypsin digestion suggests that these proteins are localized to the chloroplast stroma following import in vitro. A to G, Large-scale import reactions using either radiolabeled AtFtsZ1-1 (A), AtFtsZ2-1 (B), AtFtsZ2-2 (C), or the control proteins, SS (D), tp110-110N, truncated Tic110 (E), HPLS (F), and Tic22 (G), were incubated for 30 min at room temperature. Each import reaction was then divided into five equal fractions. H, Likewise, a large-scale binding reaction with pSS was performed and divided into five equal fractions. Individual import and binding reactions were incubated either without (−) or with 100, 250, or 500 μg trypsin mL −1 in the absence (−) or presence (+) of Triton X-100. Protease digestion reactions were incubated on ice for 30 min and then quenched. Intact chloroplasts recovered by sedimentation were solubilized in sample buffer, separated by SDS-PAGE, and analyzed by fluorography. TP represents 10% of radiolabeled translation product added to the reaction; arrows indicate precursor protein (p) and mature protein (m).

    Journal: Plant Physiology

    Article Title: Colocalization of Plastid Division Proteins in the Chloroplast Stromal Compartment Establishes a New Functional Relationship between FtsZ1 and FtsZ2 in Higher Plants 1

    doi:

    Figure Lengend Snippet: The protection of FtsZ proteins from trypsin digestion suggests that these proteins are localized to the chloroplast stroma following import in vitro. A to G, Large-scale import reactions using either radiolabeled AtFtsZ1-1 (A), AtFtsZ2-1 (B), AtFtsZ2-2 (C), or the control proteins, SS (D), tp110-110N, truncated Tic110 (E), HPLS (F), and Tic22 (G), were incubated for 30 min at room temperature. Each import reaction was then divided into five equal fractions. H, Likewise, a large-scale binding reaction with pSS was performed and divided into five equal fractions. Individual import and binding reactions were incubated either without (−) or with 100, 250, or 500 μg trypsin mL −1 in the absence (−) or presence (+) of Triton X-100. Protease digestion reactions were incubated on ice for 30 min and then quenched. Intact chloroplasts recovered by sedimentation were solubilized in sample buffer, separated by SDS-PAGE, and analyzed by fluorography. TP represents 10% of radiolabeled translation product added to the reaction; arrows indicate precursor protein (p) and mature protein (m).

    Article Snippet: Plasmids containing the AtFtsZ1-1 , AtFtsZ2-1 , AtFtsZ2-2 , pTic22 , or tp110-110N ( ) cDNA required T7 RNA Polymerase (Promega) for transcription.

    Techniques: In Vitro, Incubation, Binding Assay, Sedimentation, SDS Page