Journal: PLoS ONE
Article Title: Enhancement of DNaseI Salt Tolerance by Mimicking the Domain Structure of DNase from an Extremely Halotolerant Bacterium Thioalkalivibrio sp. K90mix
Figure Lengend Snippet: Efficiency of template DNA removal after in vitro transcription reaction using DNaseI and its fusion variants. DNaseDT denotes the fusion with the (HhH) 2 domain of DNase from an extremely salt tolerant bacterium Thioalkalivibrio sp. K90mix . DNaseBS denotes the fusion with homologous domain of ComEA protein from Bacillus subtilis . Transcription reactions were performed using TranscriptAid™ T7 High Yield Transcription Kit (Thermo Fisher Scientific, #K0441). As a template 1 μg control DNA from the kit was used. After transcription reaction (2 h, 37°C) undiluted and 5x diluted samples were treated with varying amounts of nucleases. K- denotes a control sample, which was not treated with DNase. A—denotes cases, when sample was not diluted before treatment with DNase. B—denotes cases, when sample was diluted 5 folds before treatment with DNase.
Article Snippet: Template DNA removal after in vitro transcription In vitro transcription was performed using TranscriptAid™ T7 High Yield Transcription Kit (Thermo Fisher Scientific, #K0441).
Techniques: In Vitro