t4 rna ligase buffer Search Results


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    • t4 rnl buffer  (New England Biolabs)
    96
    New England Biolabs t4 rnl buffer
    DNA adapters adenylylated using TS2126 Rnl1 are ligated to small RNA acceptors with high efficiency. ( A ) Ligation between adapter 2 adenylylated with TS2126 Rnl1 and the chimeric 5′-DNA/RNA-3′ acceptor 1 using the T4Rnl2trK227Q ligase. The expected product size is 35 nt. ( B ) Ligation of adapters 1T and 2, after adenylylation with TS2126 Rnl1, to RNA acceptor 2. ( C ) DNA adapters adenylylated with TS2126 Rnl1 do not require gel purification prior to ligation to an RNA acceptor. Ligation efficiency comparison of adapter 1T adenylylated using TS2126 Rnl1 with (lane 3 ) or without (lane 2 ) gel purification prior to T4Rnl2trK227Q ligation to acceptor 2. RNA acceptor to DNA adapter molar ratio was 1:2 in all ligations. M, ss DNA size markers; T4Rnl2trK227Q, <t>T4</t> <t>RNA</t> <t>ligase</t> 2, truncated K227Q mutant from NEB.
    T4 Rnl Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rnl buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 rnl buffer - by Bioz Stars, 2023-09
    96/100 stars
    		  Buy from Supplier
    t4 rna ligase buffer  (Thermo Fisher)
    86
    Thermo Fisher t4 rna ligase buffer
    DNA adapters adenylylated using TS2126 Rnl1 are ligated to small RNA acceptors with high efficiency. ( A ) Ligation between adapter 2 adenylylated with TS2126 Rnl1 and the chimeric 5′-DNA/RNA-3′ acceptor 1 using the T4Rnl2trK227Q ligase. The expected product size is 35 nt. ( B ) Ligation of adapters 1T and 2, after adenylylation with TS2126 Rnl1, to RNA acceptor 2. ( C ) DNA adapters adenylylated with TS2126 Rnl1 do not require gel purification prior to ligation to an RNA acceptor. Ligation efficiency comparison of adapter 1T adenylylated using TS2126 Rnl1 with (lane 3 ) or without (lane 2 ) gel purification prior to T4Rnl2trK227Q ligation to acceptor 2. RNA acceptor to DNA adapter molar ratio was 1:2 in all ligations. M, ss DNA size markers; T4Rnl2trK227Q, <t>T4</t> <t>RNA</t> <t>ligase</t> 2, truncated K227Q mutant from NEB.
    T4 Rna Ligase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase buffer/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase buffer - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier
    t4 rna ligase buffer  (New England Biolabs)
    86
    New England Biolabs t4 rna ligase buffer
    DNA adapters adenylylated using TS2126 Rnl1 are ligated to small RNA acceptors with high efficiency. ( A ) Ligation between adapter 2 adenylylated with TS2126 Rnl1 and the chimeric 5′-DNA/RNA-3′ acceptor 1 using the T4Rnl2trK227Q ligase. The expected product size is 35 nt. ( B ) Ligation of adapters 1T and 2, after adenylylation with TS2126 Rnl1, to RNA acceptor 2. ( C ) DNA adapters adenylylated with TS2126 Rnl1 do not require gel purification prior to ligation to an RNA acceptor. Ligation efficiency comparison of adapter 1T adenylylated using TS2126 Rnl1 with (lane 3 ) or without (lane 2 ) gel purification prior to T4Rnl2trK227Q ligation to acceptor 2. RNA acceptor to DNA adapter molar ratio was 1:2 in all ligations. M, ss DNA size markers; T4Rnl2trK227Q, <t>T4</t> <t>RNA</t> <t>ligase</t> 2, truncated K227Q mutant from NEB.
    T4 Rna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase buffer/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase buffer - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    DNA adapters adenylylated using TS2126 Rnl1 are ligated to small RNA acceptors with high efficiency. ( A ) Ligation between adapter 2 adenylylated with TS2126 Rnl1 and the chimeric 5′-DNA/RNA-3′ acceptor 1 using the T4Rnl2trK227Q ligase. The expected product size is 35 nt. ( B ) Ligation of adapters 1T and 2, after adenylylation with TS2126 Rnl1, to RNA acceptor 2. ( C ) DNA adapters adenylylated with TS2126 Rnl1 do not require gel purification prior to ligation to an RNA acceptor. Ligation efficiency comparison of adapter 1T adenylylated using TS2126 Rnl1 with (lane 3 ) or without (lane 2 ) gel purification prior to T4Rnl2trK227Q ligation to acceptor 2. RNA acceptor to DNA adapter molar ratio was 1:2 in all ligations. M, ss DNA size markers; T4Rnl2trK227Q, T4 RNA ligase 2, truncated K227Q mutant from NEB.

    Journal: RNA

    Article Title: Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I

    doi: 10.1261/rna.054999.115

    Figure Lengend Snippet: DNA adapters adenylylated using TS2126 Rnl1 are ligated to small RNA acceptors with high efficiency. ( A ) Ligation between adapter 2 adenylylated with TS2126 Rnl1 and the chimeric 5′-DNA/RNA-3′ acceptor 1 using the T4Rnl2trK227Q ligase. The expected product size is 35 nt. ( B ) Ligation of adapters 1T and 2, after adenylylation with TS2126 Rnl1, to RNA acceptor 2. ( C ) DNA adapters adenylylated with TS2126 Rnl1 do not require gel purification prior to ligation to an RNA acceptor. Ligation efficiency comparison of adapter 1T adenylylated using TS2126 Rnl1 with (lane 3 ) or without (lane 2 ) gel purification prior to T4Rnl2trK227Q ligation to acceptor 2. RNA acceptor to DNA adapter molar ratio was 1:2 in all ligations. M, ss DNA size markers; T4Rnl2trK227Q, T4 RNA ligase 2, truncated K227Q mutant from NEB.

    Article Snippet: Ligation reactions (10 µL) contained 1 µM acceptor RNA or DNA/RNA chimera, 2 µM adenylylated adapter, 1× T4 Rnl buffer (NEB), 200 U T4Rnl2trK227Q (NEB), and 20% PEG 8000, and were incubated overnight at 16°C.

    Techniques: Ligation, Gel Purification, Mutagenesis