t4 rna ligase buffer Search Results


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  • 98
    New England Biolabs t4 rna ligase buffer
    : briefly, (1) 5 adenylation (5’-App) that allows specific ligation of ssDNA to ssRNA with a mutated <t>T4</t> RNA ligase; (2) random barcode (blue); (3) DpnII recognition site (pink); (4) biotin for isolation (purple); (5) PacI recognition site (orange). Gray lines represent restriction enzyme cleavage patterns. (B) Schematic of the final dsDNA molecule that results from the ChAR-seq procedure. RNA and DNA in close proximity will ligate to the bridge linker molecule as shown. Red represents cDNA derived from the ssRNA and green represents genomic DNA. Dashed lines on either side represent extended sequence of varying length.
    T4 Rna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase buffer/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase buffer - by Bioz Stars, 2022-12
    98/100 stars
      Buy from Supplier

    96
    Thermo Fisher t4 rna ligase buffer
    : briefly, (1) 5 adenylation (5’-App) that allows specific ligation of ssDNA to ssRNA with a mutated <t>T4</t> RNA ligase; (2) random barcode (blue); (3) DpnII recognition site (pink); (4) biotin for isolation (purple); (5) PacI recognition site (orange). Gray lines represent restriction enzyme cleavage patterns. (B) Schematic of the final dsDNA molecule that results from the ChAR-seq procedure. RNA and DNA in close proximity will ligate to the bridge linker molecule as shown. Red represents cDNA derived from the ssRNA and green represents genomic DNA. Dashed lines on either side represent extended sequence of varying length.
    T4 Rna Ligase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase buffer/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase buffer - by Bioz Stars, 2022-12
    96/100 stars
      Buy from Supplier

    Image Search Results


    : briefly, (1) 5 adenylation (5’-App) that allows specific ligation of ssDNA to ssRNA with a mutated T4 RNA ligase; (2) random barcode (blue); (3) DpnII recognition site (pink); (4) biotin for isolation (purple); (5) PacI recognition site (orange). Gray lines represent restriction enzyme cleavage patterns. (B) Schematic of the final dsDNA molecule that results from the ChAR-seq procedure. RNA and DNA in close proximity will ligate to the bridge linker molecule as shown. Red represents cDNA derived from the ssRNA and green represents genomic DNA. Dashed lines on either side represent extended sequence of varying length.

    Journal: Current protocols in molecular biology

    Article Title: Chromatin-associated RNA sequencing (ChAR-seq)

    doi: 10.1002/cpmb.87

    Figure Lengend Snippet: : briefly, (1) 5 adenylation (5’-App) that allows specific ligation of ssDNA to ssRNA with a mutated T4 RNA ligase; (2) random barcode (blue); (3) DpnII recognition site (pink); (4) biotin for isolation (purple); (5) PacI recognition site (orange). Gray lines represent restriction enzyme cleavage patterns. (B) Schematic of the final dsDNA molecule that results from the ChAR-seq procedure. RNA and DNA in close proximity will ligate to the bridge linker molecule as shown. Red represents cDNA derived from the ssRNA and green represents genomic DNA. Dashed lines on either side represent extended sequence of varying length.

    Article Snippet: 10× T4 RNA ligase buffer (NEB, cat. no. B0216L).

    Techniques: Ligation, Isolation, Derivative Assay, Sequencing