t4 rna ligase Ge Healthcare Search Results


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  • 90
    Thermo Fisher t4 dna ligase
    T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 19341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Thermo Fisher
    Average 90 stars, based on 19341 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-02
    90/100 stars
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    90
    GE Healthcare t4 rna ligase
    Effect of various modifications on the 3′ terminal nucleotide of a small RNA on <t>T4</t> RNA ligase- and yeast PAP-catalyzed reactions. ( a ) T4 RNA ligase-mediated ligation of various miR173 forms to an RNA linker. ( b ) Activity of yeast PAP on various forms of miR173 in the presence of 2 pmol [α- 32 P]-ATP. The ladders or smears represent products of PAP-catalyzed reaction. ( c ) Activity of yeast PAP on various forms of miR173 in the presence of 10 pmol [α- 32 P]-ATP.
    T4 Rna Ligase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase/product/GE Healthcare
    Average 90 stars, based on 268 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    78
    GE Healthcare 25 u t4 rna ligase
    Effect of various modifications on the 3′ terminal nucleotide of a small RNA on <t>T4</t> RNA ligase- and yeast PAP-catalyzed reactions. ( a ) T4 RNA ligase-mediated ligation of various miR173 forms to an RNA linker. ( b ) Activity of yeast PAP on various forms of miR173 in the presence of 2 pmol [α- 32 P]-ATP. The ladders or smears represent products of PAP-catalyzed reaction. ( c ) Activity of yeast PAP on various forms of miR173 in the presence of 10 pmol [α- 32 P]-ATP.
    25 U T4 Rna Ligase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/25 u t4 rna ligase/product/GE Healthcare
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    25 u t4 rna ligase - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    79
    GE Healthcare enzyme t4 rna ligase
    Effect of various modifications on the 3′ terminal nucleotide of a small RNA on <t>T4</t> RNA ligase- and yeast PAP-catalyzed reactions. ( a ) T4 RNA ligase-mediated ligation of various miR173 forms to an RNA linker. ( b ) Activity of yeast PAP on various forms of miR173 in the presence of 2 pmol [α- 32 P]-ATP. The ladders or smears represent products of PAP-catalyzed reaction. ( c ) Activity of yeast PAP on various forms of miR173 in the presence of 10 pmol [α- 32 P]-ATP.
    Enzyme T4 Rna Ligase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme t4 rna ligase/product/GE Healthcare
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    enzyme t4 rna ligase - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

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    Effect of various modifications on the 3′ terminal nucleotide of a small RNA on T4 RNA ligase- and yeast PAP-catalyzed reactions. ( a ) T4 RNA ligase-mediated ligation of various miR173 forms to an RNA linker. ( b ) Activity of yeast PAP on various forms of miR173 in the presence of 2 pmol [α- 32 P]-ATP. The ladders or smears represent products of PAP-catalyzed reaction. ( c ) Activity of yeast PAP on various forms of miR173 in the presence of 10 pmol [α- 32 P]-ATP.

    Journal: Nucleic Acids Research

    Article Title: HEN1 recognizes 21-24 nt small RNA duplexes and deposits a methyl group onto the 2? OH of the 3? terminal nucleotide

    doi: 10.1093/nar/gkj474

    Figure Lengend Snippet: Effect of various modifications on the 3′ terminal nucleotide of a small RNA on T4 RNA ligase- and yeast PAP-catalyzed reactions. ( a ) T4 RNA ligase-mediated ligation of various miR173 forms to an RNA linker. ( b ) Activity of yeast PAP on various forms of miR173 in the presence of 2 pmol [α- 32 P]-ATP. The ladders or smears represent products of PAP-catalyzed reaction. ( c ) Activity of yeast PAP on various forms of miR173 in the presence of 10 pmol [α- 32 P]-ATP.

    Article Snippet: Ligation of the miR173 RNAs to an RNA linker (5′-pUAUGAAGCC), in which the 3′ OH is blocked by a C-3 spacer (CH2 CH2 CH2 OH), was performed at 16°C for 16 h in a 10 µl mixture containing 20 U T4 RNA ligase (Amersham Pharmacia), 1 ng of dephosphorylated miR173 and 1 µg RNA linker.

    Techniques: Ligation, Activity Assay