t4 polynucleotide kinase Promega Search Results


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  • 93
    Promega t4 polynucleotide kinase
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 5424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NEN Life Science t4 polynucleotide kinase
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega phage t4 polynucleotide kinase
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    Phage T4 Polynucleotide Kinase, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega bacteriophage t4 polynucleotide kinase
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    Bacteriophage T4 Polynucleotide Kinase, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega t4 polynucleotide kinase pnk
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase Pnk, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 polynucleotide kinase buffer
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 polynucleotide kinase kit
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 polynucleotide kinase reaction
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega t4 polynucleotide kinase labeling kit
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase Labeling Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega atpand t4 polynucleotide kinase
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    Atpand T4 Polynucleotide Kinase, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 polynucleotide kinase user manual
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinase User Manual, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Promega 1ãƒâ— t4 polynucleotide kinase buffer
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    1ãƒâ— T4 Polynucleotide Kinase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega t4 polynucleotide dna kinase
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Dna Kinase, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega 1x t4 pnk buffer
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    1x T4 Pnk Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 polynucleotide kinases
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    T4 Polynucleotide Kinases, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega p 32
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    P 32, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dna 5 end labeling kit
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    Dna 5 End Labeling Kit, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pnk reaction mix
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    Pnk Reaction Mix, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega χ32 p atp labelled probe
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    χ32 P Atp Labelled Probe, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega γ32 p labelled s rep ssrna
    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using <t>T4</t> polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.
    γ32 P Labelled S Rep Ssrna, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using T4 polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.

    Journal: Journal of Bacteriology

    Article Title: Mycobacterial Bacilli Are Metabolically Active during Chronic Tuberculosis in Murine Lungs: Insights from Genome-Wide Transcriptional Profiling ▿Mycobacterial Bacilli Are Metabolically Active during Chronic Tuberculosis in Murine Lungs: Insights from Genome-Wide Transcriptional Profiling ▿ †

    doi: 10.1128/JB.00011-07

    Figure Lengend Snippet: Transcriptional activities of in vivo-activated genes during chronic tuberculosis. (A) Organization of the rv0348 operon with the secondary structure of the Rv0348 protein. (B) EMSA of the binding ability of rRv0348 or the MBP to an upstream region within the rv0347 sequence. (C) EMSA of the binding ability of rRv0348 in the presence of both specific (rv0347) and nonspecific (map2505) probes. 32 P-labeled probes were prepared by end labeling using T4 polynucleotide kinase. Protein-DNA complexes were resolved in 4% SDS-polyacrylamide gel electrophoresis gels and exposed to X-ray films for 2 to 6 h before development. Probe names and concentrations are listed above each gel image.

    Article Snippet: The purified DNA fragments (3.5 pmol) were end labeled with 10 U of T4 polynucleotide kinase (Promega) and 10 μCi of [γ32 -P]ATP (Perkin-Elmer, Wellesley, MA) at 37°C for 10 min.

    Techniques: In Vivo, Binding Assay, Sequencing, Labeling, End Labeling, Polyacrylamide Gel Electrophoresis