t4 pnk Thermo Fisher Search Results


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  • 99
    New England Biolabs t4 pnk
    T4 Pnk, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t4 polynucleotide kinase
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    T4 Polynucleotide Kinase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8000 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t4 pnk buffer
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    T4 Pnk Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 5×t4 pnk buffer a
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    5×T4 Pnk Buffer A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 18427 013 klenow dna polymerase t4 pnk minelute reaction cleanup kit
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    18427 013 Klenow Dna Polymerase T4 Pnk Minelute Reaction Cleanup Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher γ 32 p labelled spac ssrna
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    γ 32 P Labelled Spac Ssrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t4 kinase exchange reaction
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    T4 Kinase Exchange Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher kinasemax 5 labeling kit
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    Kinasemax 5 Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher rnase h mix
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    Rnase H Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher phosphorylated
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    Phosphorylated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher γ 32 p atp end labeled oligonucleotides
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    γ 32 P Atp End Labeled Oligonucleotides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher peg
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    Peg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t4 dna ligase 5 u µl
    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by <t>T4</t> polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate
    T4 Dna Ligase 5 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by T4 polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate

    Journal: The Journal of Biological Chemistry

    Article Title: Sequence and Generation of Mature Ribosomal RNA Transcripts in Dictyostelium discoideum

    doi: 10.1074/jbc.M110.208306

    Figure Lengend Snippet: Principle of the cRT-PCR method used to determine the ends of RNA molecules. Total RNA is dephosphorylated by shrimp alkaline phosphatase ( SAP ) and then 5′-monophosphorylated by T4 polynucleotide kinase ( T4 PNK ). The resulting 5′-phosphate

    Article Snippet: After precipitation, the RNA was incubated with 20 units of T4 polynucleotide kinase (Fermentas) and 20 units of RiboLock RNase Inhibitor (Fermentas) in 30 μl of 50 m m Tris-HCl (pH 7.6), 10 m m MgCl2 , 5 m m DTT, 100 μ m spermidine, 1 m m ATP for 30 min at 37 °C.

    Techniques: Polymerase Chain Reaction

    Conversion of a single deoxybonucleotide T (red, left) in a DNA strand to a deoxyribonucleotide C (green, right) using a combination of DNAzyme F-8 and natural enzymes. DNA substrate was cleaved by F-8 to generate both 3′- and 5′-phosphate termini. Then the 3′-phosphate group was removed by T4 polynucleotide kinase (PNK), and a right DNA splint was introduced. Subsequent extension and ligation restored the original DNA except for a substitution of T with C. Inset: PAGE analysis of the various stages of the single-nucleotide excision repair process. Lane 1, the original 43-nt 5′- 32 P-labeled DNA substrate. Lane 2, the upstream cleavage product generated by F-8. The cleaved fragment migrates slightly faster than the corresponding 18-nt marker because of its 3′-phosphate group. The dotted box indicates the expected position of the downstream cleavage product, which was invisible on the autoradiogram since it contained no 32 P. Lane 3, the cleavage product after dephosphorylation with PNK. Lane 4, 5′- 32 P-labeled marker that comigrates with the 17-nt upstream cleavage fragment. Lane 5, the reaction products obtained after purifying the cleavage fragments, annealing them with right DNA splint, extending them by a C (18 nt) using DNA polymerase, and finally ligating to generate full-length substrate (43 nt). Lane 6, synthetic DNA markers. Cleavage assays were under standard single-turnover conditions (about 1 μM deoxyribozyme combined with 10 nM substrate).

    Journal: Nucleic Acids Research

    Article Title: In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity

    doi: 10.1093/nar/gku592

    Figure Lengend Snippet: Conversion of a single deoxybonucleotide T (red, left) in a DNA strand to a deoxyribonucleotide C (green, right) using a combination of DNAzyme F-8 and natural enzymes. DNA substrate was cleaved by F-8 to generate both 3′- and 5′-phosphate termini. Then the 3′-phosphate group was removed by T4 polynucleotide kinase (PNK), and a right DNA splint was introduced. Subsequent extension and ligation restored the original DNA except for a substitution of T with C. Inset: PAGE analysis of the various stages of the single-nucleotide excision repair process. Lane 1, the original 43-nt 5′- 32 P-labeled DNA substrate. Lane 2, the upstream cleavage product generated by F-8. The cleaved fragment migrates slightly faster than the corresponding 18-nt marker because of its 3′-phosphate group. The dotted box indicates the expected position of the downstream cleavage product, which was invisible on the autoradiogram since it contained no 32 P. Lane 3, the cleavage product after dephosphorylation with PNK. Lane 4, 5′- 32 P-labeled marker that comigrates with the 17-nt upstream cleavage fragment. Lane 5, the reaction products obtained after purifying the cleavage fragments, annealing them with right DNA splint, extending them by a C (18 nt) using DNA polymerase, and finally ligating to generate full-length substrate (43 nt). Lane 6, synthetic DNA markers. Cleavage assays were under standard single-turnover conditions (about 1 μM deoxyribozyme combined with 10 nM substrate).

    Article Snippet: Materials T4 polynucleotide kinase (PNK), T4 ligase and FastAPTM were purchased from MBI Fermentas.

    Techniques: Ligation, Polyacrylamide Gel Electrophoresis, Labeling, Generated, Marker, De-Phosphorylation Assay