Journal: Frontiers in Microbiology
Article Title: The Burkholderia thailandensis Phages ΦE058 and ΦE067 Represent Distinct Prototypes of a New Subgroup of Temperate Burkholderia Myoviruses
Figure Lengend Snippet: Analysis of the ΦE067 genome ends. (A) EcoRI digests of the phage DNA. Lane 1, untreated digest, lane 2, restriction pattern after heating of the digest at 80°C for 10 min and subsequent cooling on ice, lane 3, DNA that had been treated with T4 ligase before restriction. (B) Bal31 analysis. Lane 1, EcoRI digest without Bal31 (control), lane 2, ΦE067 DNA that had been treated with Bal31 (0.1 units) for 15 min before digestion with EcoRI, lane 3, same as before, but 30 min, lane 4, same as before, but 60 min, lane 5, same as before, but 120 min. (C) Comparison of the EcoRI single digest with the EcoRI/HindIII double digest. Lane 1, EcoRI restriction pattern, lane 2, EcoRI/HindIII restriction pattern; the two bands of 1,771 bp and 569 bp generated by HindIII are indicated. (D) Bal31 analysis of ΦE067 DNA that had afore been cleaved by HindIII. Lane 1, EcoRI/HindIII digest without Bal31, lane 2, ΦE067 DNA that had been treated with Bal31 (0.1 units) for 5 min before digestion with EcoRI, lane 3, same as before, but 15 min, lane 4, same as before, but 30 min, lane 5, same as before, but 60 min, lane 6, same as before, and but 120 min. The two bands of 1,771 bp and 569 bp generated by HindIII, but no other bands of the digest disappeared after 5 to 15 min of incubation with Bal31.
Article Snippet: Restriction fragments of the individual digests were treated with T4 ligase (Fisher Scientific, Schwerte, Germany) and used as templates for outward PCR.
Techniques: Generated, Incubation