t4 dna polymerase Promega Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/New England Biolabs
    Average 99 stars, based on 6737 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    95
    Promega t4 dna polymerase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna polymerase/product/Promega
    Average 95 stars, based on 862 article reviews
    Price from $9.99 to $1999.99
    t4 dna polymerase - by Bioz Stars, 2020-01
    95/100 stars
      Buy from Supplier

    78
    Promega t4 dna polymerase based dna quantitation system
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Dna Polymerase Based Dna Quantitation System, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna polymerase based dna quantitation system/product/Promega
    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    t4 dna polymerase based dna quantitation system - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    96
    Promega t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 1682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/Promega
    Average 96 stars, based on 1682 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-01
    96/100 stars
      Buy from Supplier

    98
    Promega t4 dna ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Dna Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 8206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Promega
    Average 98 stars, based on 8206 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-01
    98/100 stars
      Buy from Supplier

    89
    Promega t4 ligase buffer
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase buffer/product/Promega
    Average 89 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    t4 ligase buffer - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    86
    Promega hc t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Hc T4 Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hc t4 ligase/product/Promega
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    hc t4 ligase - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

    94
    Promega t4 dna ligase buffer
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Dna Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase buffer/product/Promega
    Average 94 stars, based on 175 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase buffer - by Bioz Stars, 2020-01
    94/100 stars
      Buy from Supplier

    83
    Promega t4 polynucleotide dna kinase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Polynucleotide Dna Kinase, supplied by Promega, used in various techniques. Bioz Stars score: 83/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide dna kinase/product/Promega
    Average 83 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide dna kinase - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    97
    Promega t4 dna ligase enzyme
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Dna Ligase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase enzyme/product/Promega
    Average 97 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase enzyme - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    76
    Promega high concentration t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    High Concentration T4 Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high concentration t4 ligase/product/Promega
    Average 76 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    high concentration t4 ligase - by Bioz Stars, 2020-01
    76/100 stars
      Buy from Supplier

    97
    Promega reaction buffer
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 3773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reaction buffer/product/Promega
    Average 97 stars, based on 3773 article reviews
    Price from $9.99 to $1999.99
    reaction buffer - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    99
    Promega rq dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rq Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq dnase i/product/Promega
    Average 99 stars, based on 292 article reviews
    Price from $9.99 to $1999.99
    rq dnase i - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    98
    Promega dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 10404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Promega
    Average 98 stars, based on 10404 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-01
    98/100 stars
      Buy from Supplier

    78
    Promega column dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Column Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/column dnase i/product/Promega
    Average 78 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    column dnase i - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    88
    Promega recombinant dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Recombinant Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant dnase i/product/Promega
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant dnase i - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    93
    Promega dnase i treatment
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Dnase I Treatment, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i treatment/product/Promega
    Average 93 stars, based on 500 article reviews
    Price from $9.99 to $1999.99
    dnase i treatment - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    99
    Promega rq1 dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rq1 Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 dnase i/product/Promega
    Average 99 stars, based on 1104 article reviews
    Price from $9.99 to $1999.99
    rq1 dnase i - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    97
    Promega rnase free dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rnase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 3552 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase i/product/Promega
    Average 97 stars, based on 3552 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase i - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    78
    Promega rnaase free dnaase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rnaase Free Dnaase I, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaase free dnaase i/product/Promega
    Average 78 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    rnaase free dnaase i - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    84
    Promega rqi rnase free dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rqi Rnase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 84/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rqi rnase free dnase i/product/Promega
    Average 84 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rqi rnase free dnase i - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    80
    Promega rnse free dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rnse Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnse free dnase i/product/Promega
    Average 80 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    rnse free dnase i - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    89
    Promega rna free dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rna Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna free dnase i/product/Promega
    Average 89 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    rna free dnase i - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    78
    Promega rnase free amplification grade dnase i
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Rnase Free Amplification Grade Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free amplification grade dnase i/product/Promega
    Average 78 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    rnase free amplification grade dnase i - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    86
    Promega dnase i reaction buffer
    NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, <t>DNAse</t> I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P
    Dnase I Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i reaction buffer/product/Promega
    Average 86 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    dnase i reaction buffer - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

    99
    Promega rq1 rnase free dnase i
    NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, <t>DNAse</t> I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P
    Rq1 Rnase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 rnase free dnase i/product/Promega
    Average 99 stars, based on 1206 article reviews
    Price from $9.99 to $1999.99
    rq1 rnase free dnase i - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    96
    New England Biolabs dntps
    NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, <t>DNAse</t> I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P
    Dntps, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 6669 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/New England Biolabs
    Average 96 stars, based on 6669 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-01
    96/100 stars
      Buy from Supplier

    78
    Promega dnase i stop solution
    NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, <t>DNAse</t> I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P
    Dnase I Stop Solution, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i stop solution/product/Promega
    Average 78 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dnase i stop solution - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    Image Search Results


    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of T4 ligase

    Journal: Analytical and bioanalytical chemistry

    Article Title: Heterobifunctional modification of DNA for conjugation to solid surfaces

    doi: 10.1007/s00216-010-3733-5

    Figure Lengend Snippet: PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of T4 ligase

    Article Snippet: We observed that, under conditions suggested by the suppliers of the T4 ligase (NEB and Promega), the predominant product was persistently a single-addition product (~100% 125-mer versus ~0% of 150-mer DNA), whereas the yield of conversion was relatively low ( > 70% of original DNA remained unmodified in spite of the 25-fold molar excess of adaptors).

    Techniques: Polyacrylamide Gel Electrophoresis, Ligation

    Analysis of PAGE data. a Image intensity profiles are averaged over 20–40 pixels in a given lane and then fitted to multiple Gaussian peaks (using IGOR Pro, Wavemetrics) representing 100-mer-, 125-mer-, and 150-mer-long DNA fragments. Peak areas (normalized by the total fragment length) are used as a quantitative measure of the molar amount of given oligomers. b Time course of changes in the molar fraction of the dA-tailed unmodified DNA (100-mer) and DNA ligated at one end (125-mer) or both ends (150-mer). Six picomoles of 100-mer DNA with 25 molar excess adapters and 400 units of T4 ligase in a total volume of 50 μL were incubated at 16 °C. c Dependence of the conversion of the dA-tailed DNA into end-modified DNA on the concentration of the enzyme for 6-h-long reactions. Six picomoles of 100-mer DNA and 25 molar excess of adapters in a total volume of 10 μL were incubated at 16 °C. Curves in b and c are fits to exponential or double-exponential functions and serve as guides to the eye

    Journal: Analytical and bioanalytical chemistry

    Article Title: Heterobifunctional modification of DNA for conjugation to solid surfaces

    doi: 10.1007/s00216-010-3733-5

    Figure Lengend Snippet: Analysis of PAGE data. a Image intensity profiles are averaged over 20–40 pixels in a given lane and then fitted to multiple Gaussian peaks (using IGOR Pro, Wavemetrics) representing 100-mer-, 125-mer-, and 150-mer-long DNA fragments. Peak areas (normalized by the total fragment length) are used as a quantitative measure of the molar amount of given oligomers. b Time course of changes in the molar fraction of the dA-tailed unmodified DNA (100-mer) and DNA ligated at one end (125-mer) or both ends (150-mer). Six picomoles of 100-mer DNA with 25 molar excess adapters and 400 units of T4 ligase in a total volume of 50 μL were incubated at 16 °C. c Dependence of the conversion of the dA-tailed DNA into end-modified DNA on the concentration of the enzyme for 6-h-long reactions. Six picomoles of 100-mer DNA and 25 molar excess of adapters in a total volume of 10 μL were incubated at 16 °C. Curves in b and c are fits to exponential or double-exponential functions and serve as guides to the eye

    Article Snippet: We observed that, under conditions suggested by the suppliers of the T4 ligase (NEB and Promega), the predominant product was persistently a single-addition product (~100% 125-mer versus ~0% of 150-mer DNA), whereas the yield of conversion was relatively low ( > 70% of original DNA remained unmodified in spite of the 25-fold molar excess of adaptors).

    Techniques: Polyacrylamide Gel Electrophoresis, Incubation, Modification, Concentration Assay

    NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, DNAse I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P

    Journal: Molecular Endocrinology

    Article Title: Neurokinin B Causes Acute GnRH Secretion and Repression of GnRH Transcription in GT1-7 GnRH Neurons

    doi: 10.1210/me.2012-1271

    Figure Lengend Snippet: NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, DNAse I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P

    Article Snippet: In brief, cell nuclei were resuspended in 1× DNase I Reaction Buffer (Promega) containing 2% glycerol, 5 U of DNase I (Promega) and incubated at 37°C for 5 minutes.

    Techniques: Activation Assay, Transfection, Luciferase, Sensitive Assay, Isolation, Real-time Polymerase Chain Reaction, Amplification

    NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, DNAse I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P

    Journal: Molecular Endocrinology

    Article Title: Neurokinin B Causes Acute GnRH Secretion and Repression of GnRH Transcription in GT1-7 GnRH Neurons

    doi: 10.1210/me.2012-1271

    Figure Lengend Snippet: NK3R may induce the long-term repression of GnRH transcription by activation of PKC and a closing of chromatin at the promoter of the GnRH gene. A, GT1-7 cells were transfected with NK3R and pretreated with DMSO or 10 μM of the broad spectrum PKC inhibitor Go6983 for 30 minutes before 8 hours cotreatment with vehicle, 30 nM senktide or 100 nM TPA. Luciferase values were internally normalized using a cotransfected β-galactosidase reporter to control for transfection efficiency, and data were normalized to an external control. Data are presented as mean fold ± SEM for n = 3 independent experiments. Comparisons were made using ANOVA with Least squares means Tukey HSD (α = .05). Levels not connected by same letter are significantly different. B, DNAse I Sensitivity Assay. GT1-7 cells were transfected with NK3R and treated with either vehicle or 30 nM senktide for 10 hours. DNA was isolated from nuclei and treated with 5 U of DNase I, then analyzed by quantitative PCR using primers specific to enhancer 1 or the promoter region of the GnRH gene (Supplemental Table 1). Amplicon quantities were normalized to undigested genomic DNA and are presented as the mean fraction of DNA remaining ± SD for n = 4 independent experiments. *Significantly different from vehicle-treated control by Student's t test ( P

    Article Snippet: DNase I was inactivated using DNase I Stop Solution (Promega) and incubated at 65°C for 10 minutes.

    Techniques: Activation Assay, Transfection, Luciferase, Sensitive Assay, Isolation, Real-time Polymerase Chain Reaction, Amplification