t4 dna ligase reaction buffer New England Biolabs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 80
    New England Biolabs new england biolabs ligase buffer
    New England Biolabs Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/new england biolabs ligase buffer/product/New England Biolabs
    Average 80 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    new england biolabs ligase buffer - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 dna ligase
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 36171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/New England Biolabs
    Average 99 stars, based on 36171 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 dna ligase reaction buffer
    T4 Dna Ligase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase reaction buffer/product/New England Biolabs
    Average 99 stars, based on 732 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase reaction buffer - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs taq dna ligase
    Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna ligase/product/New England Biolabs
    Average 99 stars, based on 1092 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    79
    New England Biolabs t7 dna ligase reaction buffer
    T7 Dna Ligase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 dna ligase reaction buffer/product/New England Biolabs
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    t7 dna ligase reaction buffer - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    94
    New England Biolabs dna ligase buffer
    Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna ligase buffer/product/New England Biolabs
    Average 94 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    dna ligase buffer - by Bioz Stars, 2020-01
    94/100 stars
      Buy from Supplier

    81
    New England Biolabs e coli dna ligase reaction buffer
    E Coli Dna Ligase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dna ligase reaction buffer/product/New England Biolabs
    Average 81 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    e coli dna ligase reaction buffer - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    99
    New England Biolabs quick ligation reaction buffer
    Quick Ligation Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick ligation reaction buffer/product/New England Biolabs
    Average 99 stars, based on 244 article reviews
    Price from $9.99 to $1999.99
    quick ligation reaction buffer - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs phi29 dna polymerase reaction buffer
    Phi29 Dna Polymerase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phi29 dna polymerase reaction buffer/product/New England Biolabs
    Average 99 stars, based on 77 article reviews
    Price from $9.99 to $1999.99
    phi29 dna polymerase reaction buffer - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 rna ligase buffer
    In vitro optimization of RNA-to-DNA ligation conditions. Upper panel , Ten pmols of 17-nt adenylated ssDNA oligonucleotide (Universal App DNA, CTGTAGGCACCATCAAT) was incubated with 5 pmols of a 17nt ssRNA test probe (TTTCGTTGGAAGCGGGA) in 1x NEB <t>T4</t> RNA Ligase Buffer with the indicated ligase (NEB Thermostable 5’ AppDNA/RNA ligase (Therm 5' Ligase), NEB T4 Rnl2tr K227Q Ligase (trT4K) or NEB T4 Rnl2tr R55K, K227Q ligase (trT4KQ)) and/or supplements (PEG, BSA, ATP, RNaseOUT). Products were then analyzed using denaturing polyacrylamide gel electrophoresis using a combination of NEB microRNA and low range ssRNA ladders and stained with SYBR-gold. Bands were quantified and the percent product was calculated using (shifted / (total * 0.66)) to account for the molar excess of DNA over RNA. No adjustment was made to account for preferential staining of ssDNA over ssRNA. Residual signal is expected in the lower band owing to the molar excess of DNA over RNA. A high molecular weight band is visible in the Therm 5’ Ligase lane, which most likely consists of high molecular weight concatemers of the AppDNA substrate caused by incomplete 3’ blocking of these oligos or removal of the 3’ block by the Therm 5’ Ligase. This experiment was performed once.
    T4 Rna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase buffer/product/New England Biolabs
    Average 99 stars, based on 311 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase buffer - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 dna polymerase
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    T4 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna polymerase/product/New England Biolabs
    Average 99 stars, based on 6313 article reviews
    Price from $9.99 to $1999.99
    t4 dna polymerase - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    98
    New England Biolabs taq dna polymerase
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 8085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/New England Biolabs
    Average 98 stars, based on 8085 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-01
    98/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 polynucleotide kinase reaction buffer
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    T4 Polynucleotide Kinase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase reaction buffer/product/New England Biolabs
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase reaction buffer - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    77
    New England Biolabs t4 rna ligase 2 reaction solution
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    T4 Rna Ligase 2 Reaction Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase 2 reaction solution/product/New England Biolabs
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase 2 reaction solution - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    99
    New England Biolabs uracil dna glycosylase udg
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    Uracil Dna Glycosylase Udg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uracil dna glycosylase udg/product/New England Biolabs
    Average 99 stars, based on 263 article reviews
    Price from $9.99 to $1999.99
    uracil dna glycosylase udg - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs phusion high fidelity pcr master mix
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity pcr master mix/product/New England Biolabs
    Average 99 stars, based on 3909 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity pcr master mix - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 polynucleotide kinase
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 22375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase/product/New England Biolabs
    Average 99 stars, based on 22375 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    81
    New England Biolabs neb end repair reaction buffer
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    Neb End Repair Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb end repair reaction buffer/product/New England Biolabs
    Average 81 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    neb end repair reaction buffer - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    99
    New England Biolabs phusion high fidelity dna polymerase
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    Phusion High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 16358 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity dna polymerase - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs neb buffer
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    Neb Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb buffer/product/New England Biolabs
    Average 99 stars, based on 634 article reviews
    Price from $9.99 to $1999.99
    neb buffer - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    92
    New England Biolabs onetaq hot start 2x master mix
    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with <t>T4</t> DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).
    Onetaq Hot Start 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq hot start 2x master mix/product/New England Biolabs
    Average 92 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    onetaq hot start 2x master mix - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    Image Search Results


    In vitro optimization of RNA-to-DNA ligation conditions. Upper panel , Ten pmols of 17-nt adenylated ssDNA oligonucleotide (Universal App DNA, CTGTAGGCACCATCAAT) was incubated with 5 pmols of a 17nt ssRNA test probe (TTTCGTTGGAAGCGGGA) in 1x NEB T4 RNA Ligase Buffer with the indicated ligase (NEB Thermostable 5’ AppDNA/RNA ligase (Therm 5' Ligase), NEB T4 Rnl2tr K227Q Ligase (trT4K) or NEB T4 Rnl2tr R55K, K227Q ligase (trT4KQ)) and/or supplements (PEG, BSA, ATP, RNaseOUT). Products were then analyzed using denaturing polyacrylamide gel electrophoresis using a combination of NEB microRNA and low range ssRNA ladders and stained with SYBR-gold. Bands were quantified and the percent product was calculated using (shifted / (total * 0.66)) to account for the molar excess of DNA over RNA. No adjustment was made to account for preferential staining of ssDNA over ssRNA. Residual signal is expected in the lower band owing to the molar excess of DNA over RNA. A high molecular weight band is visible in the Therm 5’ Ligase lane, which most likely consists of high molecular weight concatemers of the AppDNA substrate caused by incomplete 3’ blocking of these oligos or removal of the 3’ block by the Therm 5’ Ligase. This experiment was performed once.

    Journal: eLife

    Article Title: Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts

    doi: 10.7554/eLife.27024

    Figure Lengend Snippet: In vitro optimization of RNA-to-DNA ligation conditions. Upper panel , Ten pmols of 17-nt adenylated ssDNA oligonucleotide (Universal App DNA, CTGTAGGCACCATCAAT) was incubated with 5 pmols of a 17nt ssRNA test probe (TTTCGTTGGAAGCGGGA) in 1x NEB T4 RNA Ligase Buffer with the indicated ligase (NEB Thermostable 5’ AppDNA/RNA ligase (Therm 5' Ligase), NEB T4 Rnl2tr K227Q Ligase (trT4K) or NEB T4 Rnl2tr R55K, K227Q ligase (trT4KQ)) and/or supplements (PEG, BSA, ATP, RNaseOUT). Products were then analyzed using denaturing polyacrylamide gel electrophoresis using a combination of NEB microRNA and low range ssRNA ladders and stained with SYBR-gold. Bands were quantified and the percent product was calculated using (shifted / (total * 0.66)) to account for the molar excess of DNA over RNA. No adjustment was made to account for preferential staining of ssDNA over ssRNA. Residual signal is expected in the lower band owing to the molar excess of DNA over RNA. A high molecular weight band is visible in the Therm 5’ Ligase lane, which most likely consists of high molecular weight concatemers of the AppDNA substrate caused by incomplete 3’ blocking of these oligos or removal of the 3’ block by the Therm 5’ Ligase. This experiment was performed once.

    Article Snippet: Step 4B ( Optional additional step B ): RNase control Pre-mix and resuspend the pellet in the following 160 µL water 20 µL 10x T4 RNA ligase buffer 10 µL 10 mg/mL RNaseA 10 µL RNaseH (NEB) Incubate at 37C for 4 hr Centrifuge at 2.5 k x g for 2 min, discard supernatant Add 1000 µL DEPC-treated PBS, mix gently Centrifuge at 2.5 k x g for 2 min, discard supernatant Immediately proceed to the next step, with pre-mixed reaction buffer already prepared

    Techniques: In Vitro, DNA Ligation, Incubation, Polyacrylamide Gel Electrophoresis, Staining, Molecular Weight, Blocking Assay

    Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with T4 DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).

    Journal: PLoS ONE

    Article Title: Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction

    doi: 10.1371/journal.pone.0111538

    Figure Lengend Snippet: Cloning strategy. The vector contains two appropriately oriented BsaI sites (A) upon digestion with BsaI linearized vector is obtained with ends having 4-base 5′-overhangs (B) shown in red. The recognition sequence of restriction enzyme BsaI are underlined and the cleavage site is marked. The Gene Of Interest (GOI) is amplified using two gene-specific primers with 7-base long additional sequence at the 5′ end (C) shown in bold. Treatment of PCR product with T4 DNA polymerase and dTTP produces two different four-base overhangs that are complementary to two ends of the linearized vector shown in red (D). The ligation results in direction cloning of the insert into the vector (E).

    Article Snippet: Both of these enzymes are then heat-inactivated in the same tube, followed by addition of dTTP and T4 DNA polymerase without the need for adding any buffer, as T4 DNA polymerase is compatible with most buffers used for PCR.

    Techniques: Clone Assay, Plasmid Preparation, Sequencing, Amplification, Polymerase Chain Reaction, Ligation