t4 dna ligase buffer promega Search Results


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  • 93
    Promega t4 dna ligase buffer
    T4 Dna Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 ligase buffer
    T4 Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega 10x promega t4 ligase buffer
    10x Promega T4 Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega ligafast rapid dna ligation buffer
    Ligafast Rapid Dna Ligation Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dna ligase buffer
    Dna Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega ligation buffer
    Ligation Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 dna ligase
    T4 Dna Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 11518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega te buffer
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    Promega 10x t4 dna ligase buffer
    10x T4 Dna Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega 1x t4 dna ligase ligation buffer
    1x T4 Dna Ligase Ligation Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega 1xte buffer
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    Promega buffer h
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    Promega buffer
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    Promega glutathione s transferase fusion protein
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    Promega streptavidin coated magnetic beads
    Comparison between the 5fC-antibody immunoprecipitation and chemical pulldown method . (a) For the 5fC DNA immunoprecipitation we used 1 pg of 5fC-103mer and 10 pg C-103mer in the presence of 5 µg salmon sperm DNA. The immunoprecipitation resulted in an enrichment of 1.6-fold of the 5fC-103mer over the C-103mer. Error bars represent the standard error of the mean. (b) Conditions for the biotinylation reaction of a 9-mer containing a single 5fC. The oligonucleotide was incubated at room temperature with an ARP in the presence of anisidine at pH 5 for 24 h and resulted in the formation of a single product. (c) Pulldown of 1 pg 5fC-biotin-103mer in the presence of 10 pg C-103mer and 5 µg salmon sperm DNA using <t>streptavidin-coated</t> magnetic beads resulted in an enrichment of the biotinylated DNA of around 1,000-fold. Error bars represent the standard error of the mean.
    Streptavidin Coated Magnetic Beads, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1097 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 buffer dna ligase 2×
    Comparison between the 5fC-antibody immunoprecipitation and chemical pulldown method . (a) For the 5fC DNA immunoprecipitation we used 1 pg of 5fC-103mer and 10 pg C-103mer in the presence of 5 µg salmon sperm DNA. The immunoprecipitation resulted in an enrichment of 1.6-fold of the 5fC-103mer over the C-103mer. Error bars represent the standard error of the mean. (b) Conditions for the biotinylation reaction of a 9-mer containing a single 5fC. The oligonucleotide was incubated at room temperature with an ARP in the presence of anisidine at pH 5 for 24 h and resulted in the formation of a single product. (c) Pulldown of 1 pg 5fC-biotin-103mer in the presence of 10 pg C-103mer and 5 µg salmon sperm DNA using <t>streptavidin-coated</t> magnetic beads resulted in an enrichment of the biotinylated DNA of around 1,000-fold. Error bars represent the standard error of the mean.
    T4 Buffer Dna Ligase 2×, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Comparison between the 5fC-antibody immunoprecipitation and chemical pulldown method . (a) For the 5fC DNA immunoprecipitation we used 1 pg of 5fC-103mer and 10 pg C-103mer in the presence of 5 µg salmon sperm DNA. The immunoprecipitation resulted in an enrichment of 1.6-fold of the 5fC-103mer over the C-103mer. Error bars represent the standard error of the mean. (b) Conditions for the biotinylation reaction of a 9-mer containing a single 5fC. The oligonucleotide was incubated at room temperature with an ARP in the presence of anisidine at pH 5 for 24 h and resulted in the formation of a single product. (c) Pulldown of 1 pg 5fC-biotin-103mer in the presence of 10 pg C-103mer and 5 µg salmon sperm DNA using streptavidin-coated magnetic beads resulted in an enrichment of the biotinylated DNA of around 1,000-fold. Error bars represent the standard error of the mean.

    Journal: Genome Biology

    Article Title: Genome-wide distribution of 5-formylcytosine in embryonic stem cells is associated with transcription and depends on thymine DNA glycosylase

    doi: 10.1186/gb-2012-13-8-r69

    Figure Lengend Snippet: Comparison between the 5fC-antibody immunoprecipitation and chemical pulldown method . (a) For the 5fC DNA immunoprecipitation we used 1 pg of 5fC-103mer and 10 pg C-103mer in the presence of 5 µg salmon sperm DNA. The immunoprecipitation resulted in an enrichment of 1.6-fold of the 5fC-103mer over the C-103mer. Error bars represent the standard error of the mean. (b) Conditions for the biotinylation reaction of a 9-mer containing a single 5fC. The oligonucleotide was incubated at room temperature with an ARP in the presence of anisidine at pH 5 for 24 h and resulted in the formation of a single product. (c) Pulldown of 1 pg 5fC-biotin-103mer in the presence of 10 pg C-103mer and 5 µg salmon sperm DNA using streptavidin-coated magnetic beads resulted in an enrichment of the biotinylated DNA of around 1,000-fold. Error bars represent the standard error of the mean.

    Article Snippet: Following adaptor ligation, DNA and 2 μg poly-dI:dC were incubated with 50 μg streptavidin coated magnetic beads (MagneSphere Promega, Fitchburg, Wisconsin, USA) in 50 μl 2× binding buffer (10 mM Tris pH 7.5, 1 mM EDTA, 2 M NaCl, 0.1% TWEEN) for 15 minutes at room temperature.

    Techniques: Immunoprecipitation, Incubation, Magnetic Beads