t4 dna ligase Roche Search Results


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  • 99
    Qiagen hotstar hifidelity polymerase kit
    Hotstar Hifidelity Polymerase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 dna ligase
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 36171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche t4 dna ligase
    T4 Dna Ligase, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 3717 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Boehringer Mannheim t4 dna ligase
    T4 Dna Ligase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 93/100, based on 855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher u t4 dna ligase
    U T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche t4 dna ligase system
    T4 Dna Ligase System, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche rapid t4 dna ligase
    Rapid T4 Dna Ligase, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Roche t4 dna ligase enzyme
    T4 Dna Ligase Enzyme, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche dna ligase
    Dna Ligase, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Roche t7 dna ligase
    T7 Dna Ligase, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche rapid dna ligation kit
    Rapid Dna Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 2362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Roche t4 dna ligation kit
    T4 Dna Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche rapid dna dephos ligation kit
    Rapid Dna Dephos Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche klenow reaction buffer rapid dna ligation kit
    Klenow Reaction Buffer Rapid Dna Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Roche t4 ligase
    T4 Ligase, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche pwo polymerase
    Pwo Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche rapid ligation kit
    Rapid Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche phosphorylated primers
    Phosphorylated Primers, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche t4 dna polymerase
    T4 Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche hifi dna polymerase
    Hifi Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher phusion dna polymerase
    Phusion Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche first strand cdna synthetase kit
    First Strand Cdna Synthetase Kit, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche taq polymerase
    Taq Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 5339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher biotin 14 datp
    Biotin 14 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche ligation mediated pcr
    Ligation Mediated Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 98/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche klenow fragment
    Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the <t>Klenow</t> enzyme <t>(pNZ8148NK).</t> The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.
    Klenow Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Roche λ phage dna
    Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the <t>Klenow</t> enzyme <t>(pNZ8148NK).</t> The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.
    λ Phage Dna, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche gs flx short gun dna library preparation quick guide
    Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the <t>Klenow</t> enzyme <t>(pNZ8148NK).</t> The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.
    Gs Flx Short Gun Dna Library Preparation Quick Guide, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche kapa hyper dna library prep kit
    Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the <t>Klenow</t> enzyme <t>(pNZ8148NK).</t> The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.
    Kapa Hyper Dna Library Prep Kit, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore biotin labeled blunt end hairpin oligo
    Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the <t>Klenow</t> enzyme <t>(pNZ8148NK).</t> The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.
    Biotin Labeled Blunt End Hairpin Oligo, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the Klenow enzyme (pNZ8148NK). The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.

    Journal: PLoS ONE

    Article Title: Lactococcus lactis, an Alternative System for Functional Expression of Peripheral and Intrinsic Arabidopsis Membrane Proteins

    doi: 10.1371/journal.pone.0008746

    Figure Lengend Snippet: Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the Klenow enzyme (pNZ8148NK). The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.

    Article Snippet: The three independent cDNAs were excised from the pBS-RfA-cDNA vector by digestion with Eco RV and ligated into pNZ8148NK. pNZ8148NK was obtained by digestion with Nco I and subsequent treatment by Klenow fragment (Roche, Switzerland) which filled the cohesive ends.

    Techniques: Clone Assay, Plasmid Preparation, Selection, Recombinant, Ligation