t4 dna ligase New England Biolabs Search Results


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  • 99
    Thermo Fisher t4 dna ligase
    T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 18365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 dna ligase new england biolabs
    T4 Dna Ligase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs new england biolabs ligase buffer
    New England Biolabs Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa t4 dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    T4 Dna Ligase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 5252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega t4 dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    T4 Dna Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 8205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t4 dna ligase buffer
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    T4 Dna Ligase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 36171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs quick t4 dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    Quick T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 dna ligase 1
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    T4 Dna Ligase 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t7 dna ligases
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    T7 Dna Ligases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs e coli dna ligase liga
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    E Coli Dna Ligase Liga, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t3 dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    T3 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs hifi taq dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    Hifi Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 994 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna ligase buffer
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs splintr dna ligase
    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by <t>T4</t> DNA
    Splintr Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs app rna dna ligase
    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor <t>RNA</t> from an RNA <t>template/DNA</t> primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.
    App Rna Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs blunt ta dna ligase mastermix
    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor <t>RNA</t> from an RNA <t>template/DNA</t> primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.
    Blunt Ta Dna Ligase Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna t4 ligase
    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor <t>RNA</t> from an RNA <t>template/DNA</t> primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.
    Dna T4 Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs instant sticky end ligase master mix new england biolabs usa
    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor <t>RNA</t> from an RNA <t>template/DNA</t> primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.
    Instant Sticky End Ligase Master Mix New England Biolabs Usa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs ultra ii dna library prep protocol
    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor <t>RNA</t> from an RNA <t>template/DNA</t> primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.
    Ultra Ii Dna Library Prep Protocol, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs adaptor ligated fragments
    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor <t>RNA</t> from an RNA <t>template/DNA</t> primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.
    Adaptor Ligated Fragments, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs ultra ii ligation mix
    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor <t>RNA</t> from an RNA <t>template/DNA</t> primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.
    Ultra Ii Ligation Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by T4 DNA

    Journal: Nature protocols

    Article Title: Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers

    doi: 10.1038/nprot.2015.078

    Figure Lengend Snippet: Schematic illustration of noncanonical self-assembly and biomedical applications of multifunctional DNA NFs. ( a ) RCR-based assembly of NFs. The linear DNA template was first hybridized with a primer and then ligated to form a circular template by T4 DNA

    Article Snippet: Acetonitrile (CH3 CN; Sigma-Aldrich, cat. no. L810045-06) Oxidizer (0.02 M, tetrahydrofuran:H2 O:pyridine:iodine = 90.54:9.05:0.41:0.43 (vol/vol/vol/wt); Sigma-Aldrich, cat. no. L860045-06) dA-CE phosphoramidite (Glen Research, cat. no. 10-1000-10e, 1.0g) dmf-dG-CE phosphoramidite (Glen Research, cat. no. 10-1020-10e, 1.0g) dT-CE phosphoramidite (Glen Research, cat. no. 10-1030-10e, 1.0g) ac-dC-CE phosphoramidite (Glen Research, cat. no. 10-1015-10e, 1.0g) Chemical phosphorylation reagent II (CPR II; Glen Research, cat. no. 10-1901-90, 100μmoles) Thymidine lcaa CPG (1,000 Å, ChemGenes, cat. no. N-5104, 5g) Deoxyadenosine ( n -bz) 3′-lcaa CPG (1,000 Å, ChemGenes, cat. no. N-5101, 5g) Ammonium hydroxide (Sinopharm Chemical Reagent, cat. no. 10002118) Methylamine water solution (Sinopharm Chemical Reagent, cat. no. 80081228) Absolute ethanol (General-Reagent, cat. no. P1090446) Glacial acetic acid (Sinopharm Chemical Reagent, cat. no. 10000218) Triethylamine ((C2 H5 )3 N; Sigma-Aldrich, cat. no. T0886-500ML) Sodium chloride (NaCl; Adamas, cat. no. 81793B) Magnesium chloride hexahydrate (MgCl2 ·6H2 O; Sinopharm Chemical Reagent, cat. no. 10012818) Acetonitrile (CH3 CN; Adamas, cat. no. 80988C) T4 DNA ligase (400,000 units/ml; New England Biolabs, cat. no. M0202S) DNA ligation buffer, 10× (10×(50 mM Tris-HCl, 1 mM MgCl2 , 1 mM ATP and 1 mM DTT); New England Biolabs, cat. no. M0202S) dNTP (10 mM; Takara, cat. no. 4019) Cyanine 3-dUTP (1 mM; Enzo Life Sciences, cat. no. ENZ-42501) Cyanine 5-dUTP (1 mM; Enzo Life Sciences, cat. no. ENZ-42502) Fluorescein-12-ddUTP (1 mM; Enzo Life Sciences, cat. no. ENZ-42833) (6) ROX-12-ddUTP (0.1 mM; Enzo Life Sciences, cat. no. ENZ-42857) BSA, 10× (New England Biolabs, cat. no. M0269S) Phi29 DNA polymerase (10,000 units/ml; New England Biolabs, cat. no. M0269S) phi29 DNA polymerase buffer, 10× (10×(50 mM Tris-HCl, 10 mM (NH4 )2 SO4 , 10 mM MgCl2 and 4 mM DTT); New England Biolabs, cat. no. M0269S) Dulbecco’s PBS (1× DPBS basic; Gibco, cat. no. C14190500BT-500ml) Agarose (HydraGene, cat. no. R9012LE-100g) Ethidium bromide (Dingguo, cat. no. NEP028) !

    Techniques:

    Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor RNA from an RNA template/DNA primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.

    Journal: The Journal of Biological Chemistry

    Article Title: Template-switching mechanism of a group II intron-encoded reverse transcriptase and its implications for biological function and RNA-Seq

    doi: 10.1074/jbc.RA119.011337

    Figure Lengend Snippet: Models of template switching and non-templated nucleotide addition reactions. A , template switching to an acceptor RNA from an RNA template/DNA primer heteroduplex with a 1-nt 3′ overhang added by NTA after completion of cDNA synthesis or as an artificial starter duplex. B , NTA to a blunt-end RNA/DNA heteroduplex in the absence of an acceptor nucleic acid. C , template-switching to an acceptor RNA from a blunt-end RNA/DNA heteroduplex without NTA. See under “Discussion” for details.

    Article Snippet: These 3′ biases, which are restricted to the 3′-terminal nucleotide of the acceptor, account for about half of the sequence bias in TGIRT-seq, the remainder coming from the Thermostable 5′ App RNA/DNA Ligase (New England Biolabs) used for R1R adapter ligation (see A ) ( ).

    Techniques: