t. pallidum strain nichols Search Results


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  • 86
    Fujirebio serodia tppa assay
    Serodia Tppa Assay, supplied by Fujirebio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    GenScript b burgdorferi
    B314 gains ability to bind to human placental tissue sections after Tp0954 expression. (A,B) B314(pTp-Bb) strain that expressed Tp0954 on the spirochete surface efficiently binds to placental tissue sections as detected by bioluminescence measurement by IVIS-50 after addition of D-luciferin substrate for luciferase enzyme present in these spirochetes. Control B314(V) strain does not bind to placental tissue significantly confirming poor adherence ability of this strain to mammalian cells. (C) A broad overview of binding of control B314(V) labeled with FITC conjugated anti- B. <t>burgdorferi</t> antibodies by IFA showed barely detectable spirochetes on placental tissue section that was visualized by labeling with wheat germ agglutinin conjugated Alexa fluor 647 shown by red fluorescence, and (D) poor binding by these control spirochetes was confirmed by observation of sections at higher magnification. (E) A significant increase in binding to placental tissue section after Tp0954 expression in B314 strain was detected by observation of green fluorescent spirochetes using FITC-conjugated antibodies. (F) Spirochetes labeled against B. burgdorferi can be more clearly observed at higher magnification depicting significantly higher level of binding of B314(pTp-Bb) compared to B314(V) shown in (D) . (C–F) Bar on top panels indicate 100 μ and bottom panels depict 20 μ size.
    B Burgdorferi, supplied by GenScript, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher cmrl medium
    B314 gains ability to bind to human placental tissue sections after Tp0954 expression. (A,B) B314(pTp-Bb) strain that expressed Tp0954 on the spirochete surface efficiently binds to placental tissue sections as detected by bioluminescence measurement by IVIS-50 after addition of D-luciferin substrate for luciferase enzyme present in these spirochetes. Control B314(V) strain does not bind to placental tissue significantly confirming poor adherence ability of this strain to mammalian cells. (C) A broad overview of binding of control B314(V) labeled with FITC conjugated anti- B. <t>burgdorferi</t> antibodies by IFA showed barely detectable spirochetes on placental tissue section that was visualized by labeling with wheat germ agglutinin conjugated Alexa fluor 647 shown by red fluorescence, and (D) poor binding by these control spirochetes was confirmed by observation of sections at higher magnification. (E) A significant increase in binding to placental tissue section after Tp0954 expression in B314 strain was detected by observation of green fluorescent spirochetes using FITC-conjugated antibodies. (F) Spirochetes labeled against B. burgdorferi can be more clearly observed at higher magnification depicting significantly higher level of binding of B314(pTp-Bb) compared to B314(V) shown in (D) . (C–F) Bar on top panels indicate 100 μ and bottom panels depict 20 μ size.
    Cmrl Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    DNASTAR lasergene software
    B314 gains ability to bind to human placental tissue sections after Tp0954 expression. (A,B) B314(pTp-Bb) strain that expressed Tp0954 on the spirochete surface efficiently binds to placental tissue sections as detected by bioluminescence measurement by IVIS-50 after addition of D-luciferin substrate for luciferase enzyme present in these spirochetes. Control B314(V) strain does not bind to placental tissue significantly confirming poor adherence ability of this strain to mammalian cells. (C) A broad overview of binding of control B314(V) labeled with FITC conjugated anti- B. <t>burgdorferi</t> antibodies by IFA showed barely detectable spirochetes on placental tissue section that was visualized by labeling with wheat germ agglutinin conjugated Alexa fluor 647 shown by red fluorescence, and (D) poor binding by these control spirochetes was confirmed by observation of sections at higher magnification. (E) A significant increase in binding to placental tissue section after Tp0954 expression in B314 strain was detected by observation of green fluorescent spirochetes using FITC-conjugated antibodies. (F) Spirochetes labeled against B. burgdorferi can be more clearly observed at higher magnification depicting significantly higher level of binding of B314(pTp-Bb) compared to B314(V) shown in (D) . (C–F) Bar on top panels indicate 100 μ and bottom panels depict 20 μ size.
    Lasergene Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lasergene software/product/DNASTAR
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    N/A
    Treponema pallidum mouse monoclonal antibody clone BDI811 Purified
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    Image Search Results


    B314 gains ability to bind to human placental tissue sections after Tp0954 expression. (A,B) B314(pTp-Bb) strain that expressed Tp0954 on the spirochete surface efficiently binds to placental tissue sections as detected by bioluminescence measurement by IVIS-50 after addition of D-luciferin substrate for luciferase enzyme present in these spirochetes. Control B314(V) strain does not bind to placental tissue significantly confirming poor adherence ability of this strain to mammalian cells. (C) A broad overview of binding of control B314(V) labeled with FITC conjugated anti- B. burgdorferi antibodies by IFA showed barely detectable spirochetes on placental tissue section that was visualized by labeling with wheat germ agglutinin conjugated Alexa fluor 647 shown by red fluorescence, and (D) poor binding by these control spirochetes was confirmed by observation of sections at higher magnification. (E) A significant increase in binding to placental tissue section after Tp0954 expression in B314 strain was detected by observation of green fluorescent spirochetes using FITC-conjugated antibodies. (F) Spirochetes labeled against B. burgdorferi can be more clearly observed at higher magnification depicting significantly higher level of binding of B314(pTp-Bb) compared to B314(V) shown in (D) . (C–F) Bar on top panels indicate 100 μ and bottom panels depict 20 μ size.

    Journal: Frontiers in Microbiology

    Article Title: Identification and Functional Assessment of the First Placental Adhesin of Treponema pallidum That May Play Critical Role in Congenital Syphilis

    doi: 10.3389/fmicb.2020.621654

    Figure Lengend Snippet: B314 gains ability to bind to human placental tissue sections after Tp0954 expression. (A,B) B314(pTp-Bb) strain that expressed Tp0954 on the spirochete surface efficiently binds to placental tissue sections as detected by bioluminescence measurement by IVIS-50 after addition of D-luciferin substrate for luciferase enzyme present in these spirochetes. Control B314(V) strain does not bind to placental tissue significantly confirming poor adherence ability of this strain to mammalian cells. (C) A broad overview of binding of control B314(V) labeled with FITC conjugated anti- B. burgdorferi antibodies by IFA showed barely detectable spirochetes on placental tissue section that was visualized by labeling with wheat germ agglutinin conjugated Alexa fluor 647 shown by red fluorescence, and (D) poor binding by these control spirochetes was confirmed by observation of sections at higher magnification. (E) A significant increase in binding to placental tissue section after Tp0954 expression in B314 strain was detected by observation of green fluorescent spirochetes using FITC-conjugated antibodies. (F) Spirochetes labeled against B. burgdorferi can be more clearly observed at higher magnification depicting significantly higher level of binding of B314(pTp-Bb) compared to B314(V) shown in (D) . (C–F) Bar on top panels indicate 100 μ and bottom panels depict 20 μ size.

    Article Snippet: A comparable albeit slightly smaller band was detected in Nichols strain of T. pallidum (Tp) possibly due to different lipid moiety incorporated in B. burgdorferi and T. pallidum lipoproteins.

    Techniques: Expressing, Luciferase, Binding Assay, Labeling, Immunofluorescence, Fluorescence

    Expression of Tp0954 in B. burgdorferi B314 strain and detection on the surface of transformed spirochetes. (A) Schematic of the construct expressing the Tp0954 coding region driven by the B. burgdorferi ospC promoter (pTp-Bb). (B) Tp0954 expression in B314(pTp-Bb) and not vector transformed B314(V) strain was detected by RT-PCR using Tp0954 primers. Positive control RT-PCR is also shown in which primers were used to amplify the ospC gene of B. burgdorferi . RT-PCR products were absent when reverse transcriptase was not included in the reaction indicting specificity of RT-PCR. (C) Detection of three Tp0954 protein bands (marked by horizontal lines) in T. pallidum (Tp) Nichols strain as well as Tp0954 transformed B314 strain, B314(pTp-Bb) using antibodies against 281 N-terminal amino acids of mature Tp0954 protein (anti-Tp0954N) suggest differential post-translational processing of full size Tp0954 protein of ~50 kD size. *Non-specific binding with cross reactivity of antibodies with unrelated B314 proteins. (D) Limited proteinase K treatment of intact spirochetes digested only surface Tp0954 versions and B. burgdorferi OspC protein but not periplasmic flagellar protein. (E,F) IFA using anti-Tp0954N primary antibodies followed by TRITC-labeled secondary antibodies using unpermeabilized spirochetes confirmed the surface localization of Tp0954 only in Tp0954 transformed B314 and not in the control, B314(V), strain. (G,H) The lack of staining of flagella in unpermeabilized spirochetes by anti-FlaB monoclonal antibodies treatment followed by anti-mouse Alexa fluor 488 antibodies indicate that integrity of outer membrane of B314 was maintained during IFA procedure. (I,J) Staining of B. burgdorferi flagella after permeabilization with methanol indicates the specific reactivity of anti-FlaB antibodies. All spirochetes present in each microscopic field are detected by co-labeling with DNA stain, DAPI and superimposed images are shown in (E–J) . Bar indicates 20 μ size.

    Journal: Frontiers in Microbiology

    Article Title: Identification and Functional Assessment of the First Placental Adhesin of Treponema pallidum That May Play Critical Role in Congenital Syphilis

    doi: 10.3389/fmicb.2020.621654

    Figure Lengend Snippet: Expression of Tp0954 in B. burgdorferi B314 strain and detection on the surface of transformed spirochetes. (A) Schematic of the construct expressing the Tp0954 coding region driven by the B. burgdorferi ospC promoter (pTp-Bb). (B) Tp0954 expression in B314(pTp-Bb) and not vector transformed B314(V) strain was detected by RT-PCR using Tp0954 primers. Positive control RT-PCR is also shown in which primers were used to amplify the ospC gene of B. burgdorferi . RT-PCR products were absent when reverse transcriptase was not included in the reaction indicting specificity of RT-PCR. (C) Detection of three Tp0954 protein bands (marked by horizontal lines) in T. pallidum (Tp) Nichols strain as well as Tp0954 transformed B314 strain, B314(pTp-Bb) using antibodies against 281 N-terminal amino acids of mature Tp0954 protein (anti-Tp0954N) suggest differential post-translational processing of full size Tp0954 protein of ~50 kD size. *Non-specific binding with cross reactivity of antibodies with unrelated B314 proteins. (D) Limited proteinase K treatment of intact spirochetes digested only surface Tp0954 versions and B. burgdorferi OspC protein but not periplasmic flagellar protein. (E,F) IFA using anti-Tp0954N primary antibodies followed by TRITC-labeled secondary antibodies using unpermeabilized spirochetes confirmed the surface localization of Tp0954 only in Tp0954 transformed B314 and not in the control, B314(V), strain. (G,H) The lack of staining of flagella in unpermeabilized spirochetes by anti-FlaB monoclonal antibodies treatment followed by anti-mouse Alexa fluor 488 antibodies indicate that integrity of outer membrane of B314 was maintained during IFA procedure. (I,J) Staining of B. burgdorferi flagella after permeabilization with methanol indicates the specific reactivity of anti-FlaB antibodies. All spirochetes present in each microscopic field are detected by co-labeling with DNA stain, DAPI and superimposed images are shown in (E–J) . Bar indicates 20 μ size.

    Article Snippet: A comparable albeit slightly smaller band was detected in Nichols strain of T. pallidum (Tp) possibly due to different lipid moiety incorporated in B. burgdorferi and T. pallidum lipoproteins.

    Techniques: Expressing, Transformation Assay, Construct, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Positive Control, Binding Assay, Immunofluorescence, Labeling, Staining