t forsythia Atcc Search Results


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  • 99
    Qiagen bacteria mini kit
    Bacteria Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher fetal bovine serum
    Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 175586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC t forsythia atcc
    Knockout of pseC and legC decreases biofilm formation of T. forsythia <t>ATCC</t> 43037 and T. forsythia <t>UB4</t> cells, respectively. ( A ) Biofilm formation in T. forsythia ATCC 43037 wild-type compared to ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp . ( B ) Biofilm formation in T. forsythia UB4 wild-type compared to UB4 Δ legC and the complemented strain T. forsythia UB4 Δ legC comp . Data represent mean values ± SD of at least four independent experiments with four replicates each and were analyzed by the unpaired Student’s t-test. Asterisks indicate significant differences (** P
    T Forsythia Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    ATCC t forsythia 43037
    Knockout of pseC and legC decreases biofilm formation of T. forsythia <t>ATCC</t> 43037 and T. forsythia <t>UB4</t> cells, respectively. ( A ) Biofilm formation in T. forsythia ATCC 43037 wild-type compared to ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp . ( B ) Biofilm formation in T. forsythia UB4 wild-type compared to UB4 Δ legC and the complemented strain T. forsythia UB4 Δ legC comp . Data represent mean values ± SD of at least four independent experiments with four replicates each and were analyzed by the unpaired Student’s t-test. Asterisks indicate significant differences (** P
    T Forsythia 43037, supplied by ATCC, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    ATCC t forsythia 92a2
    Knockout of pseC and legC decreases biofilm formation of T. forsythia <t>ATCC</t> 43037 and T. forsythia <t>UB4</t> cells, respectively. ( A ) Biofilm formation in T. forsythia ATCC 43037 wild-type compared to ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp . ( B ) Biofilm formation in T. forsythia UB4 wild-type compared to UB4 Δ legC and the complemented strain T. forsythia UB4 Δ legC comp . Data represent mean values ± SD of at least four independent experiments with four replicates each and were analyzed by the unpaired Student’s t-test. Asterisks indicate significant differences (** P
    T Forsythia 92a2, supplied by ATCC, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    ATCC nulo deficient deletion mutants atcc
    Experimentally confirmed enzymatic steps (in green) in T. forsythia strains <t>ATCC</t> 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique <t>NulO</t> derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.
    Nulo Deficient Deletion Mutants Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC t denticola
    Experimentally confirmed enzymatic steps (in green) in T. forsythia strains <t>ATCC</t> 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique <t>NulO</t> derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.
    T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    ATCC p intermedia
    Experimentally confirmed enzymatic steps (in green) in T. forsythia strains <t>ATCC</t> 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique <t>NulO</t> derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.
    P Intermedia, supplied by ATCC, used in various techniques. Bioz Stars score: 81/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    ATCC s mitis
    Experimentally confirmed enzymatic steps (in green) in T. forsythia strains <t>ATCC</t> 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique <t>NulO</t> derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.
    S Mitis, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC oral epithelial cell line h357
    Experimentally confirmed enzymatic steps (in green) in T. forsythia strains <t>ATCC</t> 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique <t>NulO</t> derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.
    Oral Epithelial Cell Line H357, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC f nucleatum
    Descriptive analysis of adhesion of (a) A. <t>actinomycetemcomitans</t> , (b) T. forsythia , and (c) C. rectus on all groups tested (Group TiO 2 : as-annealed TiO 2 nanotubes; Group Ag: as-annealed Ag doped TiO 2 nanotubes; Group Ti: commercially pure Ti sheet; Control Group: 24-well cell culture plate bottoms). Data are presented as the mean ± SD (standard deviation). Results were analyzed using a one-way ANOVA and post hoc analyses were performed using Tukey's studentized range (HSD) test (* P
    F Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC p gingivalis
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium <t>nucleatum</t> (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus <t>mutans</t> (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    P Gingivalis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2007 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    ATCC actinomyces viscosus
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces <t>viscosus</t> (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Actinomyces Viscosus, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b suis  (ATCC)
    78
    ATCC b suis
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus <t>sobrinus</t> (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    B Suis, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    ATCC f varium
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus <t>sobrinus</t> (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    F Varium, supplied by ATCC, used in various techniques. Bioz Stars score: 81/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dmso  (ATCC)
    97
    ATCC dmso
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus <t>sobrinus</t> (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Dmso, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC eggerthella lenta
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus <t>sobrinus</t> (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Eggerthella Lenta, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC veillonella parvula
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Veillonella Parvula, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    ATCC peptostreptococcus anaerobius
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Peptostreptococcus Anaerobius, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC veillonella dispar
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Veillonella Dispar, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    ATCC pse5am7gra
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Pse5am7gra, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    ATCC capnocytophaga sputigena
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    79
    ATCC capnocytophaga gingivalis
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    96
    ATCC porphyromonas gingivalis w50
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    84
    ATCC pasteurella haemolytica
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    82
    ATCC kingella kingae
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Kingella Kingae, supplied by ATCC, used in various techniques. Bioz Stars score: 82/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    ATCC antibodies against a actinomycetemcomitans serotype b
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
    Antibodies Against A Actinomycetemcomitans Serotype B, supplied by ATCC, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Mitsubishi Gas Chemical Company gaspak system
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    Difco columbia broth
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    Illumina Inc miseq platform
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    Millipore calf thymus
    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces <t>naeslundii</t> (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.
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    Image Search Results


    Knockout of pseC and legC decreases biofilm formation of T. forsythia ATCC 43037 and T. forsythia UB4 cells, respectively. ( A ) Biofilm formation in T. forsythia ATCC 43037 wild-type compared to ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp . ( B ) Biofilm formation in T. forsythia UB4 wild-type compared to UB4 Δ legC and the complemented strain T. forsythia UB4 Δ legC comp . Data represent mean values ± SD of at least four independent experiments with four replicates each and were analyzed by the unpaired Student’s t-test. Asterisks indicate significant differences (** P

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: Knockout of pseC and legC decreases biofilm formation of T. forsythia ATCC 43037 and T. forsythia UB4 cells, respectively. ( A ) Biofilm formation in T. forsythia ATCC 43037 wild-type compared to ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp . ( B ) Biofilm formation in T. forsythia UB4 wild-type compared to UB4 Δ legC and the complemented strain T. forsythia UB4 Δ legC comp . Data represent mean values ± SD of at least four independent experiments with four replicates each and were analyzed by the unpaired Student’s t-test. Asterisks indicate significant differences (** P

    Article Snippet: Transformation of T. forsythia ATCC and T. forsythia UB4 was done as described previously ( , ).

    Techniques: Knock-Out

    Experimentally confirmed enzymatic steps (in green) in T. forsythia strains ATCC 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique NulO derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: Experimentally confirmed enzymatic steps (in green) in T. forsythia strains ATCC 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique NulO derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.

    Article Snippet: Transformation of T. forsythia ATCC and T. forsythia UB4 was done as described previously ( , ).

    Techniques: Introduce

    Phylogenetic clusters of selected microbial NulO synthase homologs. Based on the prediction of NulO sugar type by Lewis et al. (2009) , a distance-based neighbor joining tree calculated from the amino acid sequences of NulO synthase enzymes places Tanf_01240 of T. forsythia type strain ATCC 43037 in a phylogenetic clade of pseudaminic acid synthases, while BFO_1066 of strain FDC 92A2/UB4 is clustered with Leg synthases (bootstrap values shown at relevant nodes). Green denotes Leg synthases, red Neu synthases and blue Pse synthases. This figure is available in black and white in print and in color at Glycobiology online.

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: Phylogenetic clusters of selected microbial NulO synthase homologs. Based on the prediction of NulO sugar type by Lewis et al. (2009) , a distance-based neighbor joining tree calculated from the amino acid sequences of NulO synthase enzymes places Tanf_01240 of T. forsythia type strain ATCC 43037 in a phylogenetic clade of pseudaminic acid synthases, while BFO_1066 of strain FDC 92A2/UB4 is clustered with Leg synthases (bootstrap values shown at relevant nodes). Green denotes Leg synthases, red Neu synthases and blue Pse synthases. This figure is available in black and white in print and in color at Glycobiology online.

    Article Snippet: Transformation of T. forsythia ATCC and T. forsythia UB4 was done as described previously ( , ).

    Techniques:

    SDS-PAGE gels (12%) of purified recombinant enzymes of the ( A ) CMP-Pse biosynthesis pathway from T. forsythia ATCC 43037 and ( B ) CMP-Leg biosynthesis pathway from T. forsythia FDC 92A2/UB4.

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: SDS-PAGE gels (12%) of purified recombinant enzymes of the ( A ) CMP-Pse biosynthesis pathway from T. forsythia ATCC 43037 and ( B ) CMP-Leg biosynthesis pathway from T. forsythia FDC 92A2/UB4.

    Article Snippet: Transformation of T. forsythia ATCC and T. forsythia UB4 was done as described previously ( , ).

    Techniques: SDS Page, Purification, Recombinant

    SDS-PAGE analysis of T. forsythia parent, NulO-deficient, and complemented strains. ( A ) CBB stained SDS-PAGE (7.5% gel) of whole cell extracts from T. forsythia ATCC 43037, ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp , and ( B ) T. forsythia UB4 wild-type, UB4 Δ legC and the complemented strain UB4 Δ legC comp . For both T. forsythia ATCC 43037 Δ pseC and UB4 Δ legC , a downshift of the S-layer protein bands (TfsA and TfsB) could be observed, which was reverted in the complemented strains.

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: SDS-PAGE analysis of T. forsythia parent, NulO-deficient, and complemented strains. ( A ) CBB stained SDS-PAGE (7.5% gel) of whole cell extracts from T. forsythia ATCC 43037, ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp , and ( B ) T. forsythia UB4 wild-type, UB4 Δ legC and the complemented strain UB4 Δ legC comp . For both T. forsythia ATCC 43037 Δ pseC and UB4 Δ legC , a downshift of the S-layer protein bands (TfsA and TfsB) could be observed, which was reverted in the complemented strains.

    Article Snippet: Transformation of T. forsythia ATCC and T. forsythia UB4 was done as described previously ( , ).

    Techniques: SDS Page, Staining

    Experimentally confirmed enzymatic steps (in green) in T. forsythia strains ATCC 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique NulO derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: Experimentally confirmed enzymatic steps (in green) in T. forsythia strains ATCC 43037 (left) and FDC 92A2/UB4 (right) corresponding to the CMP-Pse and CMP-Leg biosynthetic pathways as elucidated in H. pylori and C. jejuni , respectively ( Schoenhofen et al. 2006b , Schoenhofen et al. 2009 ). In T. forsythia , pathways will deviate at some point to produce the unique NulO derivatives found in our strains, such as Pse5Am7Gra. These deviations would be anticipated to occur either within the NulO biosynthetic pathway or post CMP-NulO biosynthesis. Red, blue and orange highlight enzymatic steps that introduce the stereochemical differences between the two pathways and also indicate the positions altered for both hexose intermediates and final NulO. The assignment of roman numerals to each compound is consistent with label designations found throughout the text. Subscripts “P” and “L” indicate intermediates from the CMP-Pse and CMP-Leg biosynthesis pathway, respectively. For simplicity, all sugars except for the NulOs are shown in 4 C 1 form. This figure is available in black and white in print and in color at Glycobiology online.

    Article Snippet: As a first indication of altered S-layer glycan structure, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of T. forsythia ATCC 43037, UB4, and the respective NulO-deficient deletion mutants ATCC 43037 ΔpseC and UB4 ΔlegC showed a clear downshift of the glycosylated S-layer proteins TfsA and TfsB ( ).

    Techniques: Introduce

    Phylogenetic clusters of selected microbial NulO synthase homologs. Based on the prediction of NulO sugar type by Lewis et al. (2009) , a distance-based neighbor joining tree calculated from the amino acid sequences of NulO synthase enzymes places Tanf_01240 of T. forsythia type strain ATCC 43037 in a phylogenetic clade of pseudaminic acid synthases, while BFO_1066 of strain FDC 92A2/UB4 is clustered with Leg synthases (bootstrap values shown at relevant nodes). Green denotes Leg synthases, red Neu synthases and blue Pse synthases. This figure is available in black and white in print and in color at Glycobiology online.

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: Phylogenetic clusters of selected microbial NulO synthase homologs. Based on the prediction of NulO sugar type by Lewis et al. (2009) , a distance-based neighbor joining tree calculated from the amino acid sequences of NulO synthase enzymes places Tanf_01240 of T. forsythia type strain ATCC 43037 in a phylogenetic clade of pseudaminic acid synthases, while BFO_1066 of strain FDC 92A2/UB4 is clustered with Leg synthases (bootstrap values shown at relevant nodes). Green denotes Leg synthases, red Neu synthases and blue Pse synthases. This figure is available in black and white in print and in color at Glycobiology online.

    Article Snippet: As a first indication of altered S-layer glycan structure, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of T. forsythia ATCC 43037, UB4, and the respective NulO-deficient deletion mutants ATCC 43037 ΔpseC and UB4 ΔlegC showed a clear downshift of the glycosylated S-layer proteins TfsA and TfsB ( ).

    Techniques:

    SDS-PAGE analysis of T. forsythia parent, NulO-deficient, and complemented strains. ( A ) CBB stained SDS-PAGE (7.5% gel) of whole cell extracts from T. forsythia ATCC 43037, ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp , and ( B ) T. forsythia UB4 wild-type, UB4 Δ legC and the complemented strain UB4 Δ legC comp . For both T. forsythia ATCC 43037 Δ pseC and UB4 Δ legC , a downshift of the S-layer protein bands (TfsA and TfsB) could be observed, which was reverted in the complemented strains.

    Journal: Glycobiology

    Article Title: Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications

    doi: 10.1093/glycob/cww129

    Figure Lengend Snippet: SDS-PAGE analysis of T. forsythia parent, NulO-deficient, and complemented strains. ( A ) CBB stained SDS-PAGE (7.5% gel) of whole cell extracts from T. forsythia ATCC 43037, ATCC 43037 Δ pseC and the complemented strain ATCC 43037 Δ pseC comp , and ( B ) T. forsythia UB4 wild-type, UB4 Δ legC and the complemented strain UB4 Δ legC comp . For both T. forsythia ATCC 43037 Δ pseC and UB4 Δ legC , a downshift of the S-layer protein bands (TfsA and TfsB) could be observed, which was reverted in the complemented strains.

    Article Snippet: As a first indication of altered S-layer glycan structure, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of T. forsythia ATCC 43037, UB4, and the respective NulO-deficient deletion mutants ATCC 43037 ΔpseC and UB4 ΔlegC showed a clear downshift of the glycosylated S-layer proteins TfsA and TfsB ( ).

    Techniques: SDS Page, Staining

    Fluorescence- in-situ -hybridization staining of fixed multispecies biofilms showing the localization of T. forsythia ATCC 43073 WT ( a ) and the NulO deficient mutant T. forsythia ATCC 43037 ΔpseC ( b ). Red: T. forsythia ; cyan: P. gingivalis ( a )/ T. denticola ( b ); green: non-hybridized cells (DNA staining YoPro-1 + Sytox). A representative area for one disc each is shown with a top view in the middle panel and side views with the biofilm–disc interface directed towards the top view. Scale bars, ( a ) 20 µm and ( b ) 15 µm. Both T. forsythia strains can be clearly detected in the form of microcolonies at the surface of the biofilm. Alteration of the surface glycosylation does not influence the bacteria's capability to grow in the multispecies consortium.

    Journal: Interface Focus

    Article Title: Nonulosonic acids contribute to the pathogenicity of the oral bacterium Tannerella forsythia

    doi: 10.1098/rsfs.2018.0064

    Figure Lengend Snippet: Fluorescence- in-situ -hybridization staining of fixed multispecies biofilms showing the localization of T. forsythia ATCC 43073 WT ( a ) and the NulO deficient mutant T. forsythia ATCC 43037 ΔpseC ( b ). Red: T. forsythia ; cyan: P. gingivalis ( a )/ T. denticola ( b ); green: non-hybridized cells (DNA staining YoPro-1 + Sytox). A representative area for one disc each is shown with a top view in the middle panel and side views with the biofilm–disc interface directed towards the top view. Scale bars, ( a ) 20 µm and ( b ) 15 µm. Both T. forsythia strains can be clearly detected in the form of microcolonies at the surface of the biofilm. Alteration of the surface glycosylation does not influence the bacteria's capability to grow in the multispecies consortium.

    Article Snippet: Protein O -glycosylation in Tannerella forsythia implicates stringent nonulosonic acid transferases Based on the identification of CMP-NulO biosynthesis genes in all analysed genomes of pathogenic T. forsythia strains and the absence of those in the genome of the periodontal health-associated Tannerella isolate [ ], we sought to characterize the NulO transferase from strains ATCC 43037 and UB4, the glycans of which terminate in a Pse5Am7Gra and modified Leg residue, respectively [ ].

    Techniques: Fluorescence, In Situ Hybridization, Staining, Mutagenesis

    Descriptive analysis of adhesion of (a) A. actinomycetemcomitans , (b) T. forsythia , and (c) C. rectus on all groups tested (Group TiO 2 : as-annealed TiO 2 nanotubes; Group Ag: as-annealed Ag doped TiO 2 nanotubes; Group Ti: commercially pure Ti sheet; Control Group: 24-well cell culture plate bottoms). Data are presented as the mean ± SD (standard deviation). Results were analyzed using a one-way ANOVA and post hoc analyses were performed using Tukey's studentized range (HSD) test (* P

    Journal: Bioinorganic Chemistry and Applications

    Article Title: Antibacterial Activity of As-Annealed TiO2 Nanotubes Doped with Ag Nanoparticles against Periodontal Pathogens

    doi: 10.1155/2014/829496

    Figure Lengend Snippet: Descriptive analysis of adhesion of (a) A. actinomycetemcomitans , (b) T. forsythia , and (c) C. rectus on all groups tested (Group TiO 2 : as-annealed TiO 2 nanotubes; Group Ag: as-annealed Ag doped TiO 2 nanotubes; Group Ti: commercially pure Ti sheet; Control Group: 24-well cell culture plate bottoms). Data are presented as the mean ± SD (standard deviation). Results were analyzed using a one-way ANOVA and post hoc analyses were performed using Tukey's studentized range (HSD) test (* P

    Article Snippet: Antibacterial Assay The tests were performed using A. actinomycetemcomitans (ATCC 43718; ATCC, Rockville MD, USA), T. forsythia (ATCC43037A), and C. rectus (ATCC 33238).

    Techniques: Cell Culture, Standard Deviation

    SEM micrographs after adhesion of A. actinomycetemcomitans , T. forsythia , and C. rectus on the surface of (a) Group TiO 2 : as-annealed TiO 2 nanotubes; (b) Group Ag: as-annealed Ag doped TiO 2 nanotubes; (c) Group Ti: commercially pure Ti sheet.

    Journal: Bioinorganic Chemistry and Applications

    Article Title: Antibacterial Activity of As-Annealed TiO2 Nanotubes Doped with Ag Nanoparticles against Periodontal Pathogens

    doi: 10.1155/2014/829496

    Figure Lengend Snippet: SEM micrographs after adhesion of A. actinomycetemcomitans , T. forsythia , and C. rectus on the surface of (a) Group TiO 2 : as-annealed TiO 2 nanotubes; (b) Group Ag: as-annealed Ag doped TiO 2 nanotubes; (c) Group Ti: commercially pure Ti sheet.

    Article Snippet: Antibacterial Assay The tests were performed using A. actinomycetemcomitans (ATCC 43718; ATCC, Rockville MD, USA), T. forsythia (ATCC43037A), and C. rectus (ATCC 33238).

    Techniques:

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay