t denticola atcc 35405 protease prtp Search Results


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  • 76
    ATCC t denticola atcc 35405 protease prtp
    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. <t>denticola</t> . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola <t>ATCC</t> 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.
    T Denticola Atcc 35405 Protease Prtp, supplied by ATCC, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    ATCC t denticola atcc 35405
    Localization of T. <t>denticola</t> major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola <t>ATCC</t> 35405 with or without membrane permeabilization, using polyclonal antibodies raised
    T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 82/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC treponema denticola strains atcc 35404
    Localization of T. <t>denticola</t> major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola <t>ATCC</t> 35405 with or without membrane permeabilization, using polyclonal antibodies raised
    Treponema Denticola Strains Atcc 35404, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    ATCC t denticola atcc 35405 proteins
    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. <t>denticola</t> . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola <t>ATCC</t> 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.
    T Denticola Atcc 35405 Proteins, supplied by ATCC, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola atcc 35405 proteins/product/ATCC
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    76
    ATCC detergent permeabilized t denticola atcc 35405
    Localization of T. <t>denticola</t> major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola <t>ATCC</t> 35405 with or without membrane permeabilization, using polyclonal antibodies raised
    Detergent Permeabilized T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC t denticola strains
    Conservation of PrcB, PrcA and PrtP in T. <t>denticola</t> strains. Deduced amino acid sequences were aligned using Clustal-W. The consensus sequence of each protein, numbered as for 35405, is shown above the aligned sequences. Mismatches from the consensus are shaded black, and gaps in the consensus are shaded grey. Predicted PrtP active site residues (Asp 203 , His 258 and Ser 447 ) are indicated by asterisks below the consensus sequence.
    T Denticola Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    ATCC immunofluorescence assays
    Conservation of PrcB, PrcA and PrtP in T. <t>denticola</t> strains. Deduced amino acid sequences were aligned using Clustal-W. The consensus sequence of each protein, numbered as for 35405, is shown above the aligned sequences. Mismatches from the consensus are shaded black, and gaps in the consensus are shaded grey. Predicted PrtP active site residues (Asp 203 , His 258 and Ser 447 ) are indicated by asterisks below the consensus sequence.
    Immunofluorescence Assays, supplied by ATCC, used in various techniques. Bioz Stars score: 89/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    ATCC prtp c terminus
    Conservation of PrcB, PrcA and PrtP in T. <t>denticola</t> strains. Deduced amino acid sequences were aligned using Clustal-W. The consensus sequence of each protein, numbered as for 35405, is shown above the aligned sequences. Mismatches from the consensus are shaded black, and gaps in the consensus are shaded grey. Predicted PrtP active site residues (Asp 203 , His 258 and Ser 447 ) are indicated by asterisks below the consensus sequence.
    Prtp C Terminus, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Journal: PLoS ONE

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    doi: 10.1371/journal.pone.0071281

    Figure Lengend Snippet: Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Article Snippet: Amino acid alignments of T. denticola ATCC 35405 protease PrtP (TDE0762) and identified homologues.

    Techniques:

    Localization of T. denticola major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola ATCC 35405 with or without membrane permeabilization, using polyclonal antibodies raised

    Journal: Infection and Immunity

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿

    doi: 10.1128/IAI.05701-11

    Figure Lengend Snippet: Localization of T. denticola major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola ATCC 35405 with or without membrane permeabilization, using polyclonal antibodies raised

    Article Snippet: All but PrtP-N and PrcA2 were visualized on the surface of intact T. denticola ATCC 35405, though PrcA2 was detected on the surface of a T. denticola msp mutant strain.

    Techniques: Immunofluorescence

    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Journal: PLoS ONE

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    doi: 10.1371/journal.pone.0071281

    Figure Lengend Snippet: Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Article Snippet: All components of the dentilisin operon (prcB, prcA and prtP) were found in T. pedis TA4 and had amino acid identities of 48%, 56%, and 67%, respectively when aligned to the corresponding T. denticola ATCC 35405 proteins.

    Techniques:

    Localization of T. denticola major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola ATCC 35405 with or without membrane permeabilization, using polyclonal antibodies raised

    Journal: Infection and Immunity

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿

    doi: 10.1128/IAI.05701-11

    Figure Lengend Snippet: Localization of T. denticola major surface protein and protease complex polypeptides. A surface localization immunofluorescence assay was performed with T. denticola ATCC 35405 with or without membrane permeabilization, using polyclonal antibodies raised

    Article Snippet: To determine the antibody accessibility of individual protease complex polypeptides, we performed immunofluorescence assays of intact and detergent-permeabilized T. denticola ATCC 35405.

    Techniques: Immunofluorescence

    Conservation of PrcB, PrcA and PrtP in T. denticola strains. Deduced amino acid sequences were aligned using Clustal-W. The consensus sequence of each protein, numbered as for 35405, is shown above the aligned sequences. Mismatches from the consensus are shaded black, and gaps in the consensus are shaded grey. Predicted PrtP active site residues (Asp 203 , His 258 and Ser 447 ) are indicated by asterisks below the consensus sequence.

    Journal: Molecular oral microbiology

    Article Title: Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex

    doi: 10.1111/omi.12013

    Figure Lengend Snippet: Conservation of PrcB, PrcA and PrtP in T. denticola strains. Deduced amino acid sequences were aligned using Clustal-W. The consensus sequence of each protein, numbered as for 35405, is shown above the aligned sequences. Mismatches from the consensus are shaded black, and gaps in the consensus are shaded grey. Predicted PrtP active site residues (Asp 203 , His 258 and Ser 447 ) are indicated by asterisks below the consensus sequence.

    Article Snippet: To address these issues we analyzed the protease operon from diverse T. denticola strains, as well as clones of the ATCC 35405 Type strain from which the genome sequence and original Genbank prtP sequence were derived.

    Techniques: Sequencing

    Outer membrane protein expression in T. denticola 35405 and CF646. The panels consist of blots probed HisProbe reagent (left panel) or with antibodies to the T. denticola proteins or polypeptides indicated. Molecular standards in kDa are shown to the left of each blot. Lanes: 1, T. denticola CF646; 2, T. denticola 35405; 3, T. denticola CF417. In blots probed with antibodies, samples were either heated (+) or not heated (-) prior to electrophoresis.

    Journal: Molecular oral microbiology

    Article Title: Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex

    doi: 10.1111/omi.12013

    Figure Lengend Snippet: Outer membrane protein expression in T. denticola 35405 and CF646. The panels consist of blots probed HisProbe reagent (left panel) or with antibodies to the T. denticola proteins or polypeptides indicated. Molecular standards in kDa are shown to the left of each blot. Lanes: 1, T. denticola CF646; 2, T. denticola 35405; 3, T. denticola CF417. In blots probed with antibodies, samples were either heated (+) or not heated (-) prior to electrophoresis.

    Article Snippet: To address these issues we analyzed the protease operon from diverse T. denticola strains, as well as clones of the ATCC 35405 Type strain from which the genome sequence and original Genbank prtP sequence were derived.

    Techniques: Expressing, Electrophoresis

    Protease expression and activity in T. denticola strains. Panel A: Immunoblots of T. denticola strains probed with antibodies specific for components of the dentilisin complex. Blots were probed with polyclonal antibodies specific for the proteins indicated to the right of each blot. All antibodies were raised against T. denticola 35405 proteins expressed in E. coli . Strain names are listed above each lane. Approximate sizes in kDa of the reactive bands are indicated to the left of each blot. Panel B: PrtP protease activity in 5-day T. denticola cultures. Protease activity of individual strains, assayed by change in absorbance at 405 nm, is expressed as a percentage relative to the SAAPFNA activity of strain 35405. Error bars represent the standard error of four independent experiments including triplicate samples.

    Journal: Molecular oral microbiology

    Article Title: Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex

    doi: 10.1111/omi.12013

    Figure Lengend Snippet: Protease expression and activity in T. denticola strains. Panel A: Immunoblots of T. denticola strains probed with antibodies specific for components of the dentilisin complex. Blots were probed with polyclonal antibodies specific for the proteins indicated to the right of each blot. All antibodies were raised against T. denticola 35405 proteins expressed in E. coli . Strain names are listed above each lane. Approximate sizes in kDa of the reactive bands are indicated to the left of each blot. Panel B: PrtP protease activity in 5-day T. denticola cultures. Protease activity of individual strains, assayed by change in absorbance at 405 nm, is expressed as a percentage relative to the SAAPFNA activity of strain 35405. Error bars represent the standard error of four independent experiments including triplicate samples.

    Article Snippet: To address these issues we analyzed the protease operon from diverse T. denticola strains, as well as clones of the ATCC 35405 Type strain from which the genome sequence and original Genbank prtP sequence were derived.

    Techniques: Expressing, Activity Assay, Western Blot