t denticola Atcc Search Results


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  • 99
    Qiagen puregene cell kit
    Puregene Cell Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 917 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore chymostatin
    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM <t>chymostatin</t> (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    Chymostatin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC t denticola atcc 35405
    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM <t>chymostatin</t> (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    ATCC t denticola type strain atcc
    Transmission electron micrograph of T. <t>denticola</t> <t>ATCC</t> 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.
    T Denticola Type Strain Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC t denticola
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    ATCC t denticola otk
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    T Denticola Otk, supplied by ATCC, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC t denticola gm 1
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    T Denticola Gm 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    ATCC t denticola serovar
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    T Denticola Serovar, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    ATCC treponema medium
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Treponema Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    ATCC oral spirochete broth
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Oral Spirochete Broth, supplied by ATCC, used in various techniques. Bioz Stars score: 87/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    ATCC new oral spirochete
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    New Oral Spirochete, supplied by ATCC, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    ATCC paredis
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Paredis, supplied by ATCC, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    ATCC buccale
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Buccale, supplied by ATCC, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    ATCC socranskii
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Socranskii, supplied by ATCC, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    ATCC actinobacillus actinomycetemcomitans
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Actinobacillus Actinomycetemcomitans, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC t pectinovorum s1
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    T Pectinovorum S1, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC a actinomycetemcomitans
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    A Actinomycetemcomitans, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    ATCC filifactor
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Filifactor, supplied by ATCC, used in various techniques. Bioz Stars score: 84/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC p gingivalis
    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. <t>denticola</t> / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    P Gingivalis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1919 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    ATCC t vincentii
    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema <t>vincentii</t> <t>LA-1</t> (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    T Vincentii, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC t phagedenis
    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema <t>vincentii</t> <t>LA-1</t> (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    T Phagedenis, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    ATCC t forsythia 43037
    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema <t>vincentii</t> <t>LA-1</t> (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    T Forsythia 43037, supplied by ATCC, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC campylobacter
    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema <t>vincentii</t> <t>LA-1</t> (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    Campylobacter, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    ATCC porphyromonas gingivalis w50
    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema <t>vincentii</t> <t>LA-1</t> (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    Porphyromonas Gingivalis W50, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    ATCC t putidum
    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema <t>vincentii</t> <t>LA-1</t> (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    T Putidum, supplied by ATCC, used in various techniques. Bioz Stars score: 82/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    ATCC f nucleatum subspecies
    Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with single species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in human PDLFs infected with ( A , B ) F . <t>nucleatum</t> (F.n), ( C , D ) P . gingivalis (P.g), ( E , F ) T . denticola (T.d), and ( G , H ) T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values are means ± standard deviation (SD) of triplicate assays. * p
    F Nucleatum Subspecies, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Nissui Pharmaceutical gam broth
    Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with single species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in human PDLFs infected with ( A , B ) F . <t>nucleatum</t> (F.n), ( C , D ) P . gingivalis (P.g), ( E , F ) T . denticola (T.d), and ( G , H ) T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values are means ± standard deviation (SD) of triplicate assays. * p
    Gam Broth, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 99/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    JEOL jem 1210 transmission electron microscope
    Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with single species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in human PDLFs infected with ( A , B ) F . <t>nucleatum</t> (F.n), ( C , D ) P . gingivalis (P.g), ( E , F ) T . denticola (T.d), and ( G , H ) T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values are means ± standard deviation (SD) of triplicate assays. * p
    Jem 1210 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 99/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc illumina miseq
    Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with single species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in human PDLFs infected with ( A , B ) F . <t>nucleatum</t> (F.n), ( C , D ) P . gingivalis (P.g), ( E , F ) T . denticola (T.d), and ( G , H ) T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values are means ± standard deviation (SD) of triplicate assays. * p
    Illumina Miseq, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 34520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Thermo Fisher schaedler agar plates
    Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with single species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in human PDLFs infected with ( A , B ) F . <t>nucleatum</t> (F.n), ( C , D ) P . gingivalis (P.g), ( E , F ) T . denticola (T.d), and ( G , H ) T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values are means ± standard deviation (SD) of triplicate assays. * p
    Schaedler Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Journal: Infection and Immunity

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    doi: 10.1128/IAI.70.7.3982-3984.2002

    Figure Lengend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Article Snippet: T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ).

    Techniques: Incubation

    Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Journal: Infection and Immunity

    Article Title: Cytopathic Effects of the Major Surface Protein and the Chymotrypsinlike Protease of Treponema denticola

    doi:

    Figure Lengend Snippet: Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Article Snippet: The objective of the present study was to quantify and compare the adherence activity and cytotoxic effects of two important outer membrane proteins of the T. denticola type strain ATCC 35405: the pore-forming adhesin Msp, and the chymotrypsinlike protease CTLP.

    Techniques: Transmission Assay, Sonication

    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. denticola / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy

    Journal: Journal of Clinical Microbiology

    Article Title: Targeting the Treponemal Microbiome of Digital Dermatitis Infections by High-Resolution Phylogenetic Analyses and Comparison with Fluorescent In Situ Hybridization

    doi: 10.1128/JCM.00320-13

    Figure Lengend Snippet: Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. denticola / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy

    Article Snippet: Of the 20 type strains that were present in the RDP database, this primer set targeted four members, i.e., T. putidum (ATCC 700334), T. denticola (ATCC 35405), T. medium (G7201), and T. pedis (T3552B).

    Techniques:

    Transformation of pBFC into different T. denticola strains. Ten micrograms of methylated (mpBFC, top panels) or unmethylated pBFC plasmid (umpBFC, bottom panels) was electroporated into ATCC 35405, ATCC 33520, and the TdΔ911 mutant. Images were

    Journal: Applied and Environmental Microbiology

    Article Title: Disruption of a Type II Endonuclease (TDE0911) Enables Treponema denticola ATCC 35405 To Accept an Unmethylated Shuttle Vector ▿

    doi: 10.1128/AEM.00417-11

    Figure Lengend Snippet: Transformation of pBFC into different T. denticola strains. Ten micrograms of methylated (mpBFC, top panels) or unmethylated pBFC plasmid (umpBFC, bottom panels) was electroporated into ATCC 35405, ATCC 33520, and the TdΔ911 mutant. Images were

    Article Snippet: Briefly, three T. denticola strains (ATCC 35405, ATCC 33520, and the TDE0911 mutant) were grown to early logarithmic phase (optical density at 600 nm of 0.3 to 0.4), and the cells were enumerated using Petroff-Hausser counting chambers.

    Techniques: Transformation Assay, Methylation, Plasmid Preparation, Mutagenesis

    The effect of antibiotics and NO releasing nanomatrix gel. (A) Mixed antibiotics (0. 125 μg/mL of CF and MN) and four different concentrations of NO were evaluated on E . faecalis . (B) Mixed antibiotics (0.125 μg/mL of CF and MN) and four different concentrations of NO were evaluated on clinical samples. (C) Effect of antibiotics and NO (100μg/mL) releasing nanomatrix gel using C1. Live/Dead Staining Image of E . faecalis , T . denticola and clinical samples for 24 hours anaerobic culture using fluorescent microscopic image in 40X, C2. Quantitated bar graph using Image J.

    Journal: PLoS ONE

    Article Title: Effects of the nitric oxide releasing biomimetic nanomatrix gel on pulp-dentin regeneration: Pilot study

    doi: 10.1371/journal.pone.0205534

    Figure Lengend Snippet: The effect of antibiotics and NO releasing nanomatrix gel. (A) Mixed antibiotics (0. 125 μg/mL of CF and MN) and four different concentrations of NO were evaluated on E . faecalis . (B) Mixed antibiotics (0.125 μg/mL of CF and MN) and four different concentrations of NO were evaluated on clinical samples. (C) Effect of antibiotics and NO (100μg/mL) releasing nanomatrix gel using C1. Live/Dead Staining Image of E . faecalis , T . denticola and clinical samples for 24 hours anaerobic culture using fluorescent microscopic image in 40X, C2. Quantitated bar graph using Image J.

    Article Snippet: E . faecalis and T . denticola were purchased (ATCC, Manassas, VA, USA) and grown anaerobically (80% N2, 10% CO2, 10% H2) in a Coy anaerobic chamber at 37°C for at least 24 hours.

    Techniques: Staining

    Biofilm formation. T . denticola ATCC 35405 (Wild) and tde2508 -deletion mutant (Δ 2508 ) were incubated on a polystyrene plate for 2 days. After washing, the adherent cells were collected and quantified using the optical density (OD) at 600 nm (A). Data represent means and standard deviations of two experiments performed in triplicate. Additionally, the adherent cells were observed by scanning electron microscopy (B).

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: Biofilm formation. T . denticola ATCC 35405 (Wild) and tde2508 -deletion mutant (Δ 2508 ) were incubated on a polystyrene plate for 2 days. After washing, the adherent cells were collected and quantified using the optical density (OD) at 600 nm (A). Data represent means and standard deviations of two experiments performed in triplicate. Additionally, the adherent cells were observed by scanning electron microscopy (B).

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques: Mutagenesis, Incubation, Electron Microscopy

    Adherence to human gingival epithelial cells. T. denticola ATCC 35405 (Wild) and tde2508 -deletion mutant (Δ 2508 ) were incubated with Ca9-22 epithelial cells for 1 h at 400 and 4,000 MOI. After immunostaining, the cells were observed by the confocal laser scanning microscopy (A). T. denticola cells and actin of the epithelial cells are shown as green and red, respectively. Each field corresponds to 0.045 mm 2 . Panel (A) shows the adherence at 4,000 MOI. We performed experiments independently twice in duplicate and counted the number of adherent T . denticola cells in 10 fields. Panel (B) shows the means and standard deviations of the number.

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: Adherence to human gingival epithelial cells. T. denticola ATCC 35405 (Wild) and tde2508 -deletion mutant (Δ 2508 ) were incubated with Ca9-22 epithelial cells for 1 h at 400 and 4,000 MOI. After immunostaining, the cells were observed by the confocal laser scanning microscopy (A). T. denticola cells and actin of the epithelial cells are shown as green and red, respectively. Each field corresponds to 0.045 mm 2 . Panel (A) shows the adherence at 4,000 MOI. We performed experiments independently twice in duplicate and counted the number of adherent T . denticola cells in 10 fields. Panel (B) shows the means and standard deviations of the number.

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques: Mutagenesis, Incubation, Immunostaining, Confocal Laser Scanning Microscopy

    Confirmation of TDE2508 abolition in the tde2508 -deletion mutant. The whole cell lysates of T . denticola ATCC 35405 (lanes 1 and 1–2 in panels A and B, respectively) and tde2508 -deletion mutant (lanes 2 and 3–4 in panels A and B, respectively) were subjected to SDS-PAGE with CBB-staining (A) and Western blot analysis with anti-TDE2508 antiserum (B). Samples were denatured by heating at 100°C for 10 min, except for lanes 2 and 4 in panel B; these two were treated without heating. The single and double black arrowheads denote a monomer and a complex forms of TDE2508, respectively. M denotes a standard marker.

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: Confirmation of TDE2508 abolition in the tde2508 -deletion mutant. The whole cell lysates of T . denticola ATCC 35405 (lanes 1 and 1–2 in panels A and B, respectively) and tde2508 -deletion mutant (lanes 2 and 3–4 in panels A and B, respectively) were subjected to SDS-PAGE with CBB-staining (A) and Western blot analysis with anti-TDE2508 antiserum (B). Samples were denatured by heating at 100°C for 10 min, except for lanes 2 and 4 in panel B; these two were treated without heating. The single and double black arrowheads denote a monomer and a complex forms of TDE2508, respectively. M denotes a standard marker.

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques: Mutagenesis, SDS Page, Staining, Western Blot, Marker

    TDE2508 complex formation. (A) The whole cell lysate of T . denticola ATCC 35405 was subjected to Western blot analysis with anti-TDE2508 antiserum. Samples in lanes 1–5 were denatured by heating at 100, 80, 60, 37, and 24°C, respectively for 10 min. (B) T. denticola intact cells in lanes 1–6 were treated with 0, 0, 10, 50, 100, and 200 mM of a chemical cross-linker, respectively. The treated cells were sonicated and denatured by heating at 60°C for lane 1 or 100°C for lanes 2–6 for 10 min, and subjected to Western blot analysis with anti-TDE2508 antiserum. The single and double black arrowheads denote a monomer and a complex form of TDE2508, respectively. M denotes a standard marker.

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: TDE2508 complex formation. (A) The whole cell lysate of T . denticola ATCC 35405 was subjected to Western blot analysis with anti-TDE2508 antiserum. Samples in lanes 1–5 were denatured by heating at 100, 80, 60, 37, and 24°C, respectively for 10 min. (B) T. denticola intact cells in lanes 1–6 were treated with 0, 0, 10, 50, 100, and 200 mM of a chemical cross-linker, respectively. The treated cells were sonicated and denatured by heating at 60°C for lane 1 or 100°C for lanes 2–6 for 10 min, and subjected to Western blot analysis with anti-TDE2508 antiserum. The single and double black arrowheads denote a monomer and a complex form of TDE2508, respectively. M denotes a standard marker.

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques: Western Blot, Sonication, Marker

    Growth curves of T . denticola . T . denticola ATCC 35405 was inoculated in mGAM-TS (5% serum, circle), TYGVS (10% serum, triangle), and mNOS (10% serum, square), and anaerobically incubated at 37°C. The optical density (OD) at 600 nm was monitored. The figure shows a representative image from one of three independent experiments.

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: Growth curves of T . denticola . T . denticola ATCC 35405 was inoculated in mGAM-TS (5% serum, circle), TYGVS (10% serum, triangle), and mNOS (10% serum, square), and anaerobically incubated at 37°C. The optical density (OD) at 600 nm was monitored. The figure shows a representative image from one of three independent experiments.

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques: Incubation

    Major membrane proteins on an SDS-PAGE gel. Ten micrograms of the envelope fraction (Env) of T . denticola ATCC 35405 was separated by SDS-PAGE and stained with CBB. The numbers (1–16) indicate proteins identified by mass spectrometry. Annotation of each protein is shown in Table 2 . M denotes a standard marker.

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: Major membrane proteins on an SDS-PAGE gel. Ten micrograms of the envelope fraction (Env) of T . denticola ATCC 35405 was separated by SDS-PAGE and stained with CBB. The numbers (1–16) indicate proteins identified by mass spectrometry. Annotation of each protein is shown in Table 2 . M denotes a standard marker.

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques: SDS Page, Staining, Mass Spectrometry, Marker

    Subcellular localization of TDE2508. The whole cell lysate (WCL), soluble fraction (Sol) and envelope fraction (Env) of T . denticola ATCC 35405 were separated by SDS-PAGE with CBB-staining (A) and analyzed by Western blotting with anti-TDE2508 antiserum (B). The envelope fraction was further fractionated by differential solubilization in 0, 0.25, 0.5, 1, 2, 4, and 8% Triton X-100, and the soluble fractions (lanes 1–7, respectively) and insoluble fractions (lanes 8–14, respectively) were subjected to Western blot analysis with anti- T . denticola whole cell antiserum (C) and anti-TDE2508 antiserum (D). The grey, white and black arrowheads denote Msp, TmpC and TDE2508, respectively. M denotes a standard marker.

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: Subcellular localization of TDE2508. The whole cell lysate (WCL), soluble fraction (Sol) and envelope fraction (Env) of T . denticola ATCC 35405 were separated by SDS-PAGE with CBB-staining (A) and analyzed by Western blotting with anti-TDE2508 antiserum (B). The envelope fraction was further fractionated by differential solubilization in 0, 0.25, 0.5, 1, 2, 4, and 8% Triton X-100, and the soluble fractions (lanes 1–7, respectively) and insoluble fractions (lanes 8–14, respectively) were subjected to Western blot analysis with anti- T . denticola whole cell antiserum (C) and anti-TDE2508 antiserum (D). The grey, white and black arrowheads denote Msp, TmpC and TDE2508, respectively. M denotes a standard marker.

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques: SDS Page, Staining, Western Blot, Marker

    Gene map of tde2508 and its neighbors on the chromosomal DNA of T . denticola ATCC 35405. The small arrows show primers which are presented in Table 1 . The tde2508 and tde2509 genes were co-transcribed (data not shown).

    Journal: PLoS ONE

    Article Title: Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola

    doi: 10.1371/journal.pone.0089051

    Figure Lengend Snippet: Gene map of tde2508 and its neighbors on the chromosomal DNA of T . denticola ATCC 35405. The small arrows show primers which are presented in Table 1 . The tde2508 and tde2509 genes were co-transcribed (data not shown).

    Article Snippet: BLAST search showed that TDE2508 exists in other T . denticola strains including ATCC 33520, MYR-T, H1-T, AL-2, ASLM, US-Trep, F0402, and H-22, and there are also homologous proteins in T . socranskii and T . azotonutricium , but not in T. pallidum .

    Techniques:

    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.

    Journal: BMC Microbiology

    Article Title: Multilocus sequence analysis of Treponema denticola strains of diverse origin

    doi: 10.1186/1471-2180-13-24

    Figure Lengend Snippet: Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.

    Article Snippet: To date, only two complete genome sequences are available for oral spirochete bacteria; those of T . denticola ATCC 35405 (type strain) [ ] and Treponema vincentii LA-1 (ATCC 35580), which has been sequenced by researchers at the J. Craig Venter Institute as part of the Human Microbiome Project [ ], but is as yet unpublished.

    Techniques:

    Phylogenetic trees of Treponema denticola strains based on a concatenated 7 - gene dataset ( flaA , recA , pyrH , ppnK , dnaN , era and radC ), using Maximum Likelihood and Bayesian methods. A : Maximum likelihood (ML) tree generated under the GTR + I + G substitution model, with bootstrap values shown above branches. The scale bar represents 0.015 nucleotide changes per site. Numbers beneath the breakpoints in the branches indicate the respective nucleotide changes per site that have been removed. B : Ultrametric Bayesian (BA) 50% majority-rule consensus tree of 9,000 trees following the removal of 1,000 trees as burn-in. Numbers above branches are posterior probabilities. The respective clades formed in each tree are indicated with a Roman numeral (I-VI). Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups.

    Journal: BMC Microbiology

    Article Title: Multilocus sequence analysis of Treponema denticola strains of diverse origin

    doi: 10.1186/1471-2180-13-24

    Figure Lengend Snippet: Phylogenetic trees of Treponema denticola strains based on a concatenated 7 - gene dataset ( flaA , recA , pyrH , ppnK , dnaN , era and radC ), using Maximum Likelihood and Bayesian methods. A : Maximum likelihood (ML) tree generated under the GTR + I + G substitution model, with bootstrap values shown above branches. The scale bar represents 0.015 nucleotide changes per site. Numbers beneath the breakpoints in the branches indicate the respective nucleotide changes per site that have been removed. B : Ultrametric Bayesian (BA) 50% majority-rule consensus tree of 9,000 trees following the removal of 1,000 trees as burn-in. Numbers above branches are posterior probabilities. The respective clades formed in each tree are indicated with a Roman numeral (I-VI). Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups.

    Article Snippet: To date, only two complete genome sequences are available for oral spirochete bacteria; those of T . denticola ATCC 35405 (type strain) [ ] and Treponema vincentii LA-1 (ATCC 35580), which has been sequenced by researchers at the J. Craig Venter Institute as part of the Human Microbiome Project [ ], but is as yet unpublished.

    Techniques: Generated

    Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with single species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in human PDLFs infected with ( A , B ) F . nucleatum (F.n), ( C , D ) P . gingivalis (P.g), ( E , F ) T . denticola (T.d), and ( G , H ) T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values are means ± standard deviation (SD) of triplicate assays. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Litsea japonica Leaf Extract Suppresses Proinflammatory Cytokine Production in Periodontal Ligament Fibroblasts Stimulated with Oral Pathogenic Bacteria or Interleukin-1β

    doi: 10.3390/ijms19092494

    Figure Lengend Snippet: Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with single species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in human PDLFs infected with ( A , B ) F . nucleatum (F.n), ( C , D ) P . gingivalis (P.g), ( E , F ) T . denticola (T.d), and ( G , H ) T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values are means ± standard deviation (SD) of triplicate assays. * p

    Article Snippet: The F. nucleatum subspecies polymorphum American Type Culture Collection (ATCC) 10953T , P. gingivalis KCOM 2804, T. denticola ATCC 35405T , and T. forsythia ATCC 43037T were provided by the Korean Collection for Oral Microbiology (Gwangju, Korea) and purchased from ATCC (Manassas, VA, USA).

    Techniques: Expressing, Infection, Standard Deviation

    LJLE inhibits pro-inflammatory cytokine expression in mixed red complex bacteria, including F . nucleatum (F.n)-infected human PDLFs. Human PDLFs were stimulated with LPS in the absence or presence of 100 μg/mL LJLE for different times (0, 1, 2, 3, 6, 12, and 24 h). Real-time PCR was performed as described in the Materials and Methods. ( A ) Time-course of effects of LJLE on IL-6 and ( B ) IL-8 mRNA expression in mixed red complex ( P . gingivalis , T . denticola , and T . forsythia ), including F. nucleatum (F.n)-infected PDLFs. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Litsea japonica Leaf Extract Suppresses Proinflammatory Cytokine Production in Periodontal Ligament Fibroblasts Stimulated with Oral Pathogenic Bacteria or Interleukin-1β

    doi: 10.3390/ijms19092494

    Figure Lengend Snippet: LJLE inhibits pro-inflammatory cytokine expression in mixed red complex bacteria, including F . nucleatum (F.n)-infected human PDLFs. Human PDLFs were stimulated with LPS in the absence or presence of 100 μg/mL LJLE for different times (0, 1, 2, 3, 6, 12, and 24 h). Real-time PCR was performed as described in the Materials and Methods. ( A ) Time-course of effects of LJLE on IL-6 and ( B ) IL-8 mRNA expression in mixed red complex ( P . gingivalis , T . denticola , and T . forsythia ), including F. nucleatum (F.n)-infected PDLFs. * p

    Article Snippet: The F. nucleatum subspecies polymorphum American Type Culture Collection (ATCC) 10953T , P. gingivalis KCOM 2804, T. denticola ATCC 35405T , and T. forsythia ATCC 43037T were provided by the Korean Collection for Oral Microbiology (Gwangju, Korea) and purchased from ATCC (Manassas, VA, USA).

    Techniques: Expressing, Infection, Real-time Polymerase Chain Reaction

    Pretreatment with LJLE inhibits pro-inflammatory cytokine expression in LPS or F . nucleatum -infected human PDLFs in a concentration-dependent manner. Human PDLFs were pretreated for 2 h with 0, 10, 50, or 100 μg/mL LJLE, followed by stimulation with E . coli LPS or F. nucleatum (MOI 100) for 24 h. Concentration-dependent inhibitory effect of LJLE pretreatment on IL-6 and IL-8 mRNA on ( A , B ) E. coli LPS-stimulated and ( C , D ) F. nucleatum -infected PDLFs. ** p

    Journal: International Journal of Molecular Sciences

    Article Title: Litsea japonica Leaf Extract Suppresses Proinflammatory Cytokine Production in Periodontal Ligament Fibroblasts Stimulated with Oral Pathogenic Bacteria or Interleukin-1β

    doi: 10.3390/ijms19092494

    Figure Lengend Snippet: Pretreatment with LJLE inhibits pro-inflammatory cytokine expression in LPS or F . nucleatum -infected human PDLFs in a concentration-dependent manner. Human PDLFs were pretreated for 2 h with 0, 10, 50, or 100 μg/mL LJLE, followed by stimulation with E . coli LPS or F. nucleatum (MOI 100) for 24 h. Concentration-dependent inhibitory effect of LJLE pretreatment on IL-6 and IL-8 mRNA on ( A , B ) E. coli LPS-stimulated and ( C , D ) F. nucleatum -infected PDLFs. ** p

    Article Snippet: The F. nucleatum subspecies polymorphum American Type Culture Collection (ATCC) 10953T , P. gingivalis KCOM 2804, T. denticola ATCC 35405T , and T. forsythia ATCC 43037T were provided by the Korean Collection for Oral Microbiology (Gwangju, Korea) and purchased from ATCC (Manassas, VA, USA).

    Techniques: Expressing, Infection, Concentration Assay

    Pretreatment with LJLE inhibits pro-inflammatory cytokine secretion in LPS- or F . nucleatum -infected human PDLFs in a concentration-dependent manner. Human PDLFs were pretreated for 2 h with 0, 10, 50, or 100 μg/mL LJLE, followed by stimulation with E . coli LPS or F. nucleatum (MOI 100) for 24 h, and supernatants were analyzed using enzyme-linked immunosorbent assay (ELISA) to detect the presence of IL-6 and IL-8. Concentration-dependent inhibitory effect of LJLE pretreatment on IL-6 and IL-8 protein expression in ( A , B ) E. coli LPS-stimulated and ( C , D ) F. nucleatum -infected PDLFs. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Litsea japonica Leaf Extract Suppresses Proinflammatory Cytokine Production in Periodontal Ligament Fibroblasts Stimulated with Oral Pathogenic Bacteria or Interleukin-1β

    doi: 10.3390/ijms19092494

    Figure Lengend Snippet: Pretreatment with LJLE inhibits pro-inflammatory cytokine secretion in LPS- or F . nucleatum -infected human PDLFs in a concentration-dependent manner. Human PDLFs were pretreated for 2 h with 0, 10, 50, or 100 μg/mL LJLE, followed by stimulation with E . coli LPS or F. nucleatum (MOI 100) for 24 h, and supernatants were analyzed using enzyme-linked immunosorbent assay (ELISA) to detect the presence of IL-6 and IL-8. Concentration-dependent inhibitory effect of LJLE pretreatment on IL-6 and IL-8 protein expression in ( A , B ) E. coli LPS-stimulated and ( C , D ) F. nucleatum -infected PDLFs. * p

    Article Snippet: The F. nucleatum subspecies polymorphum American Type Culture Collection (ATCC) 10953T , P. gingivalis KCOM 2804, T. denticola ATCC 35405T , and T. forsythia ATCC 43037T were provided by the Korean Collection for Oral Microbiology (Gwangju, Korea) and purchased from ATCC (Manassas, VA, USA).

    Techniques: Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing

    Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with dual species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in PDLFs infected with ( A , B ) F . nucleatum (F.n) plus P . gingivalis (P.g), ( C , D ) F. nucleatum plus T . denticola (T.d), and ( E , F ) F. nucleatum plus T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values represent means ± standard deviation (SD) of triplicate assays. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Litsea japonica Leaf Extract Suppresses Proinflammatory Cytokine Production in Periodontal Ligament Fibroblasts Stimulated with Oral Pathogenic Bacteria or Interleukin-1β

    doi: 10.3390/ijms19092494

    Figure Lengend Snippet: Inhibitory effect of LJLE on proinflammatory cytokine expression in human PDLFs infected with dual species of oral bacteria at different time points (0, 1, 2, 3, 6, 12, and 24 h). IL-6 and IL-8 mRNA expression in PDLFs infected with ( A , B ) F . nucleatum (F.n) plus P . gingivalis (P.g), ( C , D ) F. nucleatum plus T . denticola (T.d), and ( E , F ) F. nucleatum plus T . forsythia (T.f) in the absence or presence of 100 μg/mL LJLE. Values represent means ± standard deviation (SD) of triplicate assays. * p

    Article Snippet: The F. nucleatum subspecies polymorphum American Type Culture Collection (ATCC) 10953T , P. gingivalis KCOM 2804, T. denticola ATCC 35405T , and T. forsythia ATCC 43037T were provided by the Korean Collection for Oral Microbiology (Gwangju, Korea) and purchased from ATCC (Manassas, VA, USA).

    Techniques: Expressing, Infection, Standard Deviation