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    DSMZ t denticola dsm14222 genomic dna
    T Denticola Dsm14222 Genomic Dna, supplied by DSMZ, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t denticola dsm14222 genomic dna - by Bioz Stars, 2020-01
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    79
    Softberry Inc t denticola genome sequence
    Interaction of PrcB with PrtP. Arrows denote PrcB, PrtP, and rabbit IgG heavy chains (H). (A) Immunoblots probed with anti-PrtP antibodies and HisProbe reagent, as indicated. Lanes 1 and 2, T. <t>denticola</t> CF499; lanes 3 and 4, CF417; lanes 1 and 4, cell extracts; lanes 2 and 3, anti-PrtP immunoprecipitates from cell extracts. (B) T. denticola CF499 lysates were immunoprecipitated with polyclonal antibodies to PrtP, PrcA, FlaA, or Msp. Eluted proteins on duplicate blots were probed with anti-PrtP antibodies and HisProbe reagent, as indicated. (C) Immunoblot probed with anti-PrtP antibodies. Lanes 1 to 3, T. denticola CF499; lanes 4 to 6, T. denticola CF417; lanes 1 and 4, whole-cell extracts; lanes 2 and 5, anti-6×His monoclonal antibody immunoprecipitates from cell extracts; lanes 3 and 6, anti-PrtP antibody immunoprecipitates from cell extracts. (D) E. coli cells expressing PrtP, 6×His-PrcB, or both PrtP and 6×His-PrcB were analyzed directly and after Ni 2+ chromatography. Immunoblots of whole-cell extracts and Ni 2+ affinity column eluates were probed with anti-6×His monoclonal antibody or anti-PrtP polyclonal antibodies. Lanes: 1, E. coli /pCF411 (expresses PrtP); 2, E. coli /pCF415 (expresses PrcB-6×His); 3, E. coli /pCF416 (expresses PrcB-6×His + PrtP).
    T Denticola Genome Sequence, supplied by Softberry Inc, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola genome sequence/product/Softberry Inc
    Average 79 stars, based on 14 article reviews
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    t denticola genome sequence - by Bioz Stars, 2020-01
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    Image Search Results


    Interaction of PrcB with PrtP. Arrows denote PrcB, PrtP, and rabbit IgG heavy chains (H). (A) Immunoblots probed with anti-PrtP antibodies and HisProbe reagent, as indicated. Lanes 1 and 2, T. denticola CF499; lanes 3 and 4, CF417; lanes 1 and 4, cell extracts; lanes 2 and 3, anti-PrtP immunoprecipitates from cell extracts. (B) T. denticola CF499 lysates were immunoprecipitated with polyclonal antibodies to PrtP, PrcA, FlaA, or Msp. Eluted proteins on duplicate blots were probed with anti-PrtP antibodies and HisProbe reagent, as indicated. (C) Immunoblot probed with anti-PrtP antibodies. Lanes 1 to 3, T. denticola CF499; lanes 4 to 6, T. denticola CF417; lanes 1 and 4, whole-cell extracts; lanes 2 and 5, anti-6×His monoclonal antibody immunoprecipitates from cell extracts; lanes 3 and 6, anti-PrtP antibody immunoprecipitates from cell extracts. (D) E. coli cells expressing PrtP, 6×His-PrcB, or both PrtP and 6×His-PrcB were analyzed directly and after Ni 2+ chromatography. Immunoblots of whole-cell extracts and Ni 2+ affinity column eluates were probed with anti-6×His monoclonal antibody or anti-PrtP polyclonal antibodies. Lanes: 1, E. coli /pCF411 (expresses PrtP); 2, E. coli /pCF415 (expresses PrcB-6×His); 3, E. coli /pCF416 (expresses PrcB-6×His + PrtP).

    Journal: Journal of Bacteriology

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿

    doi: 10.1128/JB.00274-10

    Figure Lengend Snippet: Interaction of PrcB with PrtP. Arrows denote PrcB, PrtP, and rabbit IgG heavy chains (H). (A) Immunoblots probed with anti-PrtP antibodies and HisProbe reagent, as indicated. Lanes 1 and 2, T. denticola CF499; lanes 3 and 4, CF417; lanes 1 and 4, cell extracts; lanes 2 and 3, anti-PrtP immunoprecipitates from cell extracts. (B) T. denticola CF499 lysates were immunoprecipitated with polyclonal antibodies to PrtP, PrcA, FlaA, or Msp. Eluted proteins on duplicate blots were probed with anti-PrtP antibodies and HisProbe reagent, as indicated. (C) Immunoblot probed with anti-PrtP antibodies. Lanes 1 to 3, T. denticola CF499; lanes 4 to 6, T. denticola CF417; lanes 1 and 4, whole-cell extracts; lanes 2 and 5, anti-6×His monoclonal antibody immunoprecipitates from cell extracts; lanes 3 and 6, anti-PrtP antibody immunoprecipitates from cell extracts. (D) E. coli cells expressing PrtP, 6×His-PrcB, or both PrtP and 6×His-PrcB were analyzed directly and after Ni 2+ chromatography. Immunoblots of whole-cell extracts and Ni 2+ affinity column eluates were probed with anti-6×His monoclonal antibody or anti-PrtP polyclonal antibodies. Lanes: 1, E. coli /pCF411 (expresses PrtP); 2, E. coli /pCF415 (expresses PrcB-6×His); 3, E. coli /pCF416 (expresses PrcB-6×His + PrtP).

    Article Snippet: Annotation of the prcB open reading frame was done using the FGENESB algorithm trained to the T. denticola genome sequence (Softberry, Inc., Mt.

    Techniques: Western Blot, Immunoprecipitation, Expressing, Chromatography, Affinity Column

    Protease locus in T. denticola parent and prcB mutant strains. The genes of the protease operon, prcB , prcA , and prtP , are shown as gray arrows. The location of the protease operon promoter region is indicated by “P.” The protease mRNA transcript is shown as a thin arrow above the genes transcribed in each strain. The location and orientation of the erm cassette are shown by a black arrow. The T. denticola strains are 35405 (wild-type parent), P0760 ( erm insertion at the 5′ end of prcB ), CF417 ( prcB modified to encode a C-terminal His tag; erm insertion replaces the 5′ end of prcA ), CF499 ( prcB modified to encode a C-terminal His tag; erm insertion is upstream of the protease locus), and CF522 (Δ prcB ; promoter region is intact; erm insertion as in CF499).

    Journal: Journal of Bacteriology

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿

    doi: 10.1128/JB.00274-10

    Figure Lengend Snippet: Protease locus in T. denticola parent and prcB mutant strains. The genes of the protease operon, prcB , prcA , and prtP , are shown as gray arrows. The location of the protease operon promoter region is indicated by “P.” The protease mRNA transcript is shown as a thin arrow above the genes transcribed in each strain. The location and orientation of the erm cassette are shown by a black arrow. The T. denticola strains are 35405 (wild-type parent), P0760 ( erm insertion at the 5′ end of prcB ), CF417 ( prcB modified to encode a C-terminal His tag; erm insertion replaces the 5′ end of prcA ), CF499 ( prcB modified to encode a C-terminal His tag; erm insertion is upstream of the protease locus), and CF522 (Δ prcB ; promoter region is intact; erm insertion as in CF499).

    Article Snippet: Annotation of the prcB open reading frame was done using the FGENESB algorithm trained to the T. denticola genome sequence (Softberry, Inc., Mt.

    Techniques: Mutagenesis, Modification

    Conservation of prcB in T. denticola . The deduced amino acid sequence of the complete ORF, including the PrcB coding region, is shown for T. denticola strains ATCC 35405, ATCC 33520, and OTK, aligned using Clustal 2.0. The N-terminal valine residue predicted by JCVI (Val 38 ) and the two possible N-terminal methionine residues (Met 1 and Met 7 ) predicted by the present study are shaded gray. The predicted signal peptidase II cleavage site is indicated by a black arrowhead. Amino acid identity throughout all three sequences is indicated by asterisks. Conserved and semiconserved amino acids are shown by colons and dots, respectively.

    Journal: Journal of Bacteriology

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿

    doi: 10.1128/JB.00274-10

    Figure Lengend Snippet: Conservation of prcB in T. denticola . The deduced amino acid sequence of the complete ORF, including the PrcB coding region, is shown for T. denticola strains ATCC 35405, ATCC 33520, and OTK, aligned using Clustal 2.0. The N-terminal valine residue predicted by JCVI (Val 38 ) and the two possible N-terminal methionine residues (Met 1 and Met 7 ) predicted by the present study are shaded gray. The predicted signal peptidase II cleavage site is indicated by a black arrowhead. Amino acid identity throughout all three sequences is indicated by asterisks. Conserved and semiconserved amino acids are shown by colons and dots, respectively.

    Article Snippet: Annotation of the prcB open reading frame was done using the FGENESB algorithm trained to the T. denticola genome sequence (Softberry, Inc., Mt.

    Techniques: Sequencing

    Expression and localization of PrcB. PrcB-6×His and PrtP were detected on blots by use of HisProbe reagent and anti-PrtP antibodies, respectively. Triton X-114 extracts (TX-114) were partitioned into aqueous (Aq) and detergent (Det) phases. Molecular mass standards are shown in panels B and C. (A) Expression of PrcB-6×His detected in lysates of E. coli /pCF415 ( E.c. ) and T. denticola strains CF499 and CF417. (B) PrcB-6×His localizes to the detergent phase of a T. denticola CF499 Triton X-114 extract. (C) T. denticola CF499 Triton X-114 extract showing differential phase partitioning of full-length and N-terminally truncated PrcB-6×His. Samples were heated (+) or not heated (−) prior to SDS-PAGE.

    Journal: Journal of Bacteriology

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿

    doi: 10.1128/JB.00274-10

    Figure Lengend Snippet: Expression and localization of PrcB. PrcB-6×His and PrtP were detected on blots by use of HisProbe reagent and anti-PrtP antibodies, respectively. Triton X-114 extracts (TX-114) were partitioned into aqueous (Aq) and detergent (Det) phases. Molecular mass standards are shown in panels B and C. (A) Expression of PrcB-6×His detected in lysates of E. coli /pCF415 ( E.c. ) and T. denticola strains CF499 and CF417. (B) PrcB-6×His localizes to the detergent phase of a T. denticola CF499 Triton X-114 extract. (C) T. denticola CF499 Triton X-114 extract showing differential phase partitioning of full-length and N-terminally truncated PrcB-6×His. Samples were heated (+) or not heated (−) prior to SDS-PAGE.

    Article Snippet: Annotation of the prcB open reading frame was done using the FGENESB algorithm trained to the T. denticola genome sequence (Softberry, Inc., Mt.

    Techniques: Expressing, SDS Page