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    Ipsen Group synaptotagmin
    Neuronal expression of BARP. (A) Expression of BARP in brain (a) and pancreatic islets (b). Sections of cortex (1), hippocampus (2), and cerebellum (3) stained for BARP (mAb 12B1) and analyzed by immunofluorescence microscopy. BARP is detected in cell bodies and the dendritic extensions of Purkinje (white arrows) and pyramidal (green arrow) cells. In pancreatic islets, BARP is expressed in insulin-positive β cells but absent from α cells labeled for glucagon. The inset shows a higher magnification of the Purkinje cell layer. (B) Neuronal expression of BARP. Primary cells isolated from hippocampus or cerebellum were costained with mAb 12B1 to BARP and Ab to the neuronal marker MAP2 (a), the glial marker GFAP (b), the Purkinje marker calbindin (d), or the dense core vesicle protein BDNF (f). The insets show a higher magnification of the vesicular staining in the soma. Nuclei were stained with Hoechst (blue). (C) Colocalization of BARP with <t>synaptotagmin</t> in PC12 cells. Control or NGF-differentiated PC12 cells were costained with Ab to the regulated secretory vesicle marker synaptotagmin and BARP (Ab 72) and analyzed by confocal microscopy.
    Synaptotagmin, supplied by Ipsen Group, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Ipsen Group synaptotagmin 1
    Immunostaining of <t>synaptotagmin-1</t> and -2 in NMJ. A , All endplates express synaptotagmin-2 in wild-type (WT) NMJ. B , No synaptotagmin-2 was detected in synaptotagmin-2 KO NMJ. C , D , Synaptotagmin-1 was detected in some (bottom panel) but not other endplates (top panel) in both wild type and synaptotagmin-2 KO. Scale bar, 10 μm (applies to all subpanels). Abbreviations: Syt 1, Synaptotagmin-1; Syt 2, synaptotagmin-2; α-bgx, α-bungarotoxin.
    Synaptotagmin 1, supplied by Ipsen Group, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Synaptic Systems synaptotagmin i
    Immunostaining of <t>synaptotagmin-1</t> and -2 in NMJ. A , All endplates express synaptotagmin-2 in wild-type (WT) NMJ. B , No synaptotagmin-2 was detected in synaptotagmin-2 KO NMJ. C , D , Synaptotagmin-1 was detected in some (bottom panel) but not other endplates (top panel) in both wild type and synaptotagmin-2 KO. Scale bar, 10 μm (applies to all subpanels). Abbreviations: Syt 1, Synaptotagmin-1; Syt 2, synaptotagmin-2; α-bgx, α-bungarotoxin.
    Synaptotagmin I, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Synaptic Systems synaptotagmin
    Immunostaining of <t>synaptotagmin-1</t> and -2 in NMJ. A , All endplates express synaptotagmin-2 in wild-type (WT) NMJ. B , No synaptotagmin-2 was detected in synaptotagmin-2 KO NMJ. C , D , Synaptotagmin-1 was detected in some (bottom panel) but not other endplates (top panel) in both wild type and synaptotagmin-2 KO. Scale bar, 10 μm (applies to all subpanels). Abbreviations: Syt 1, Synaptotagmin-1; Syt 2, synaptotagmin-2; α-bgx, α-bungarotoxin.
    Synaptotagmin, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptotagmin/product/Synaptic Systems
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    Price from $9.99 to $1999.99
    synaptotagmin - by Bioz Stars, 2022-10
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    Image Search Results


    Neuronal expression of BARP. (A) Expression of BARP in brain (a) and pancreatic islets (b). Sections of cortex (1), hippocampus (2), and cerebellum (3) stained for BARP (mAb 12B1) and analyzed by immunofluorescence microscopy. BARP is detected in cell bodies and the dendritic extensions of Purkinje (white arrows) and pyramidal (green arrow) cells. In pancreatic islets, BARP is expressed in insulin-positive β cells but absent from α cells labeled for glucagon. The inset shows a higher magnification of the Purkinje cell layer. (B) Neuronal expression of BARP. Primary cells isolated from hippocampus or cerebellum were costained with mAb 12B1 to BARP and Ab to the neuronal marker MAP2 (a), the glial marker GFAP (b), the Purkinje marker calbindin (d), or the dense core vesicle protein BDNF (f). The insets show a higher magnification of the vesicular staining in the soma. Nuclei were stained with Hoechst (blue). (C) Colocalization of BARP with synaptotagmin in PC12 cells. Control or NGF-differentiated PC12 cells were costained with Ab to the regulated secretory vesicle marker synaptotagmin and BARP (Ab 72) and analyzed by confocal microscopy.

    Journal: The Journal of Cell Biology

    Article Title: BARP suppresses voltage-gated calcium channel activity and Ca2+-evoked exocytosis

    doi: 10.1083/jcb.201304101

    Figure Lengend Snippet: Neuronal expression of BARP. (A) Expression of BARP in brain (a) and pancreatic islets (b). Sections of cortex (1), hippocampus (2), and cerebellum (3) stained for BARP (mAb 12B1) and analyzed by immunofluorescence microscopy. BARP is detected in cell bodies and the dendritic extensions of Purkinje (white arrows) and pyramidal (green arrow) cells. In pancreatic islets, BARP is expressed in insulin-positive β cells but absent from α cells labeled for glucagon. The inset shows a higher magnification of the Purkinje cell layer. (B) Neuronal expression of BARP. Primary cells isolated from hippocampus or cerebellum were costained with mAb 12B1 to BARP and Ab to the neuronal marker MAP2 (a), the glial marker GFAP (b), the Purkinje marker calbindin (d), or the dense core vesicle protein BDNF (f). The insets show a higher magnification of the vesicular staining in the soma. Nuclei were stained with Hoechst (blue). (C) Colocalization of BARP with synaptotagmin in PC12 cells. Control or NGF-differentiated PC12 cells were costained with Ab to the regulated secretory vesicle marker synaptotagmin and BARP (Ab 72) and analyzed by confocal microscopy.

    Article Snippet: VGCCs interact via the Cav α1 subunit with several pre- and postsynaptic proteins, including SNAP-25, synaptotagmin, syntaxin, Mint, and calcium/calmodulin-dependent serine protein kinase ( ; ; ; ; ; ; ).

    Techniques: Expressing, Staining, Immunofluorescence, Microscopy, Labeling, Isolation, Marker, Confocal Microscopy

    Immunostaining of synaptotagmin-1 and -2 in NMJ. A , All endplates express synaptotagmin-2 in wild-type (WT) NMJ. B , No synaptotagmin-2 was detected in synaptotagmin-2 KO NMJ. C , D , Synaptotagmin-1 was detected in some (bottom panel) but not other endplates (top panel) in both wild type and synaptotagmin-2 KO. Scale bar, 10 μm (applies to all subpanels). Abbreviations: Syt 1, Synaptotagmin-1; Syt 2, synaptotagmin-2; α-bgx, α-bungarotoxin.

    Journal: The Journal of Neuroscience

    Article Title: Synaptotagmin-2 Is Essential for Survival and Contributes to Ca2+ Triggering of Neurotransmitter Release in Central and Neuromuscular Synapses

    doi: 10.1523/JNEUROSCI.3519-06.2006

    Figure Lengend Snippet: Immunostaining of synaptotagmin-1 and -2 in NMJ. A , All endplates express synaptotagmin-2 in wild-type (WT) NMJ. B , No synaptotagmin-2 was detected in synaptotagmin-2 KO NMJ. C , D , Synaptotagmin-1 was detected in some (bottom panel) but not other endplates (top panel) in both wild type and synaptotagmin-2 KO. Scale bar, 10 μm (applies to all subpanels). Abbreviations: Syt 1, Synaptotagmin-1; Syt 2, synaptotagmin-2; α-bgx, α-bungarotoxin.

    Article Snippet: Different effects on fast exocytosis induced by synaptotagmin-1 and 2 isoforms and abundance, but not by phosphorylation.

    Techniques: Immunostaining