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    Santa Cruz Biotechnology synpo
    Nphs2-, <t>Nphs1-</t> and <t>Synpo</t> staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.
    Synpo, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synpo/product/Santa Cruz Biotechnology
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    synpo - by Bioz Stars, 2022-08
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    86
    Progen Biotechnik synaptopodin
    Nphs2-, <t>Nphs1-</t> and <t>Synpo</t> staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.
    Synaptopodin, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptopodin/product/Progen Biotechnik
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    synaptopodin - by Bioz Stars, 2022-08
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    86
    Santa Cruz Biotechnology anti synpo
    Nphs2-, <t>Nphs1-</t> and <t>Synpo</t> staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.
    Anti Synpo, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti synpo/product/Santa Cruz Biotechnology
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    anti synpo - by Bioz Stars, 2022-08
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    86
    Abcam synaptopodin
    The expression of uPAR in urinary podocytes of patients with IgAN. a <t>Synaptopodin-negative</t> cells (white arrow). b uPAR-negative cells (white arrow). c Synaptopodin-positive cells (white arrowhead). d uPAR-positive cells (white arrowhead). e Merged cells indicating the colocalization between synaptopodin and uPAR in IgAN podocytes. a , b ×20. c–e ×40.
    Synaptopodin, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptopodin/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    synaptopodin - by Bioz Stars, 2022-08
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    Image Search Results


    Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.

    Article Snippet: Primary antibodies were as follows: TUBB2B, mouse polyclonal antibody AM09375PU-N (1:100; Acris, Herford, Germany); DCDC2, rabbit polyclonal antibody HPA031582 (1:200; Sigma, Munich, Germany); WT1, mouse monoclonal antibody Clone 6F-H2 (1:50; DAKO, Hamburg, Germany); NPHS2, rabbit polyclonal antibody ab93650 (1:200; Abcam, Cambringe, UK); NPHS1, rabbit polyclonal antibody SAB3500770 (1:200; Sigma, Munich, Germany) and SYNPO, goat polyclonal antibody sc-21537 (1:100; Santa Cruz, Heidelberg, Germany).

    Techniques: Staining, Immunohistochemistry, Mouse Assay, Labeling

    Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.

    Article Snippet: Primary antibodies were as follows: TUBB2B, mouse polyclonal antibody AM09375PU-N (1:100; Acris, Herford, Germany); DCDC2, rabbit polyclonal antibody HPA031582 (1:200; Sigma, Munich, Germany); WT1, mouse monoclonal antibody Clone 6F-H2 (1:50; DAKO, Hamburg, Germany); NPHS2, rabbit polyclonal antibody ab93650 (1:200; Abcam, Cambringe, UK); NPHS1, rabbit polyclonal antibody SAB3500770 (1:200; Sigma, Munich, Germany) and SYNPO, goat polyclonal antibody sc-21537 (1:100; Santa Cruz, Heidelberg, Germany).

    Techniques: Staining, Immunohistochemistry, Mouse Assay, Expressing

    The expression of uPAR in urinary podocytes of patients with IgAN. a Synaptopodin-negative cells (white arrow). b uPAR-negative cells (white arrow). c Synaptopodin-positive cells (white arrowhead). d uPAR-positive cells (white arrowhead). e Merged cells indicating the colocalization between synaptopodin and uPAR in IgAN podocytes. a , b ×20. c–e ×40.

    Journal: Nephron Extra

    Article Title: In IgA Nephropathy, Glomerulosclerosis Is Associated with Increased Urinary CD80 Excretion and Urokinase-Type Plasminogen Activator Receptor-Positive Podocyturia

    doi: 10.1159/000473888

    Figure Lengend Snippet: The expression of uPAR in urinary podocytes of patients with IgAN. a Synaptopodin-negative cells (white arrow). b uPAR-negative cells (white arrow). c Synaptopodin-positive cells (white arrowhead). d uPAR-positive cells (white arrowhead). e Merged cells indicating the colocalization between synaptopodin and uPAR in IgAN podocytes. a , b ×20. c–e ×40.

    Article Snippet: Podocytes were identified by immunofluorescence using synaptopodin as the primary antibody (1: 100, Abcam, Cambridge, MA, USA) and IgG anti-rabbit Alexa Fluor® 488 (1: 100, Abcam) as the secondary antibody.

    Techniques: Expressing