Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: HG-induced TrkA phosphorylation is associated with podocyte inflammation and injury. (A–D) Representative western blots show the levels of (A) TrkA and phospho-TrkA (Tyr490); (B) ERK, phospho-ERK and EGR1; (C) p35 and CDK5; and (D) IL-1β and TNF-α in MPC5 cells from the LG, HG, and LG + Man groups at 24 h (E) Representative immunofluorescence double staining shows the expression of phospho-TrkA (Tyr490) and synaptopodin in MPC5 cells from the LG, HG, and LG + Man groups. ****p < 0.0001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Antibodies against ERK (Cat. No. sc-514302), phospho-ERK (Cat. No. sc-7383), and Synaptopodin (Cat. No. sc-515842) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Phospho-proteomics, Western Blot, Immunofluorescence, Double Staining, Expressing

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibition alleviates inflammation and injury in the HG-stimulated MPC5 cells. Representative western blots show the expression levels of (A) TNF-α and IL-1β, and (G) synaptopodin in the LG, HG+Vehicle, HG + Inh-TrkA, HG + empty vector, HG + TrkA OE, and HG + TrkA OE + PD98059 groups. Representative western blots show the expression levels of (D) TNF-α; IL-1β, and (I) synaptopodin in the LG, HG+si-NC, HG+si-TrkA, HG + TrkA OE+Ctrl, and HG + TrkA OE + GW441756 groups. Quantitative analysis shows the relative gene expression levels of (B) TNF-α; (C) IL-6; and (H) Nephrin in the LG, HG+Vehicle, LG + Man, HG + Inh-TrkA, HG + empty vector, HG + TrkA OE, and HG + TrkA OE + PD98059 groups. (E) TNF-α; (F) IL-1β; and (J) Nephrin in the LG, HG+si-NC, HG+si-TrkA, HG + TrkA OE+Ctrl, and HG + TrkA OE + GW441756 groups. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p < 0.05 was considered statistically significant.

Article Snippet: Antibodies against ERK (Cat. No. sc-514302), phospho-ERK (Cat. No. sc-7383), and Synaptopodin (Cat. No. sc-515842) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Inhibition, Western Blot, Expressing, Plasmid Preparation, Gene Expression

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibition reduces the levels of p-TrkA, p-ERK, EGR1, p35, and CDK5 in the kidneys of db/db mice. (A–C) Representative western blots show the protein expression levels of (A) TrkA and phospho-TrkA (Tyr490), (B) ERK, p-ERK and EGR1, and (C) p35 and CDK5 in the kidney samples from the control, db/db, Inh-NC, and Inh-TrkA groups of mice. (D) Representative IF images show the expression of Synaptopodin (red) in the glomeruli of control, db/db, Inh-NC, and Inh-TrkA groups of mice (400X). ***p < 0.001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Antibodies against ERK (Cat. No. sc-514302), phospho-ERK (Cat. No. sc-7383), and Synaptopodin (Cat. No. sc-515842) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Inhibition, Western Blot, Expressing, Control

Journal: Cellular and molecular life sciences : CMLS

Article Title: Deficiency of the Src homology phosphatase 2 in podocytes is associated with renoprotective effects in mice under hyperglycemia

doi: 10.1007/s00018-022-04517-6

Figure Lengend Snippet: Upregulation of Shp2 in the kidney and E11 podocyte cell line under hyperglycemia. a,b Immunoblots of Shp2 and Actin (a), and Ptpn11 mRNA expression (b) in kidneys from male mice fed with regular chow (Ctrl), HFD (24 weeks), or challenged with STZ (160 μg/g body weight, 20 weeks). Actin served as a protein loading control for immunoblotting (n=4 per group), and mRNA expression was normalized to Tbp (n=4 per group). Each lane in immunoblot represents a sample from an individual mouse. *p ≤ 0.05 indicates a significant difference between Ctrl versus STZ-treated and HFD-fed mice. c Confocal images of kidney sections from Ctrl, HFD-fed (24 weeks), and STZ-treated (160 μg/g body weight, 20 weeks) wild-type male mice co-immunostained for Shp2 (green) and Synaptopodin (Synpo, red). Scale bar: 20 μm. The boxed areas in the Merge are enlarged in the lower panel. d Differentiated E11 podocytes were treated with high glucose (25 mM; HG) for 0, 24, 48, and 72 hours. Cell lysates were immunoblotted for Shp2, Synpo, and Actin. The protein expression level was normalized to Actin from three independent experiments. Statistical significance in (d) was considered *p ≤ 0.05 for Shp2 and # p ≤ 0.05 for Synpo between 0h and 24h, 48h, and 72 h. A.U.: arbitrary unit.

Article Snippet: Sections were stained using antibodies for Shp2 (sc-280), Nephrin (sc-19000) (both from Santa Cruz Biotechnology), Synaptopodin (Novus Biologicals; NBP2–33357), pAKT Ser473 (4060), pERK1/2 Tyr202/Thr204 (4370) (both from Cell Signaling Technology), and Collagen III (Abcam; ab7778) at 4°C overnight.

Techniques: Western Blot, Expressing, Control

Journal: Journal of Translational Medicine

Article Title: The expression of NOD2, NLRP3 and NLRC5 and renal injury in anti-neutrophil cytoplasmic antibody-associated vasculitis

doi: 10.1186/s12967-019-1949-5

Figure Lengend Snippet: Double immunofluorescence staining of NOD2 and glomerular intrinsic cells in AAV patients. a Co-localization of NOD2 (red) and CD31 (green). b Co-localization of NOD2 (red) and integrin-α (green). c Co-localization of NOD2 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right

Article Snippet: Glomerular endothelial cells, mesangial cells and podocytes were identified using immunofluorescence staining with primary antibodies against CD31 (1:50, sc53411; Santa Cruz Biotechnology, CA), integrin-α8 (1:50, sc365798; Santa Cruz Biotechnology, CA) and synaptopodin (1:50, MAB8977; R&D Systems, Minneapolis, MN, USA) respectively.

Techniques: Double Immunofluorescence Staining

Journal: Journal of Translational Medicine

Article Title: The expression of NOD2, NLRP3 and NLRC5 and renal injury in anti-neutrophil cytoplasmic antibody-associated vasculitis

doi: 10.1186/s12967-019-1949-5

Figure Lengend Snippet: Double immunofluorescence staining of NLRP3 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRP3 (red) and CD31 (green). b Co-localization of NLRP3 (red) and integrin-α (green). c Co-localization of NLRP3 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right

Article Snippet: Glomerular endothelial cells, mesangial cells and podocytes were identified using immunofluorescence staining with primary antibodies against CD31 (1:50, sc53411; Santa Cruz Biotechnology, CA), integrin-α8 (1:50, sc365798; Santa Cruz Biotechnology, CA) and synaptopodin (1:50, MAB8977; R&D Systems, Minneapolis, MN, USA) respectively.

Techniques: Double Immunofluorescence Staining

Journal: Journal of Translational Medicine

Article Title: The expression of NOD2, NLRP3 and NLRC5 and renal injury in anti-neutrophil cytoplasmic antibody-associated vasculitis

doi: 10.1186/s12967-019-1949-5

Figure Lengend Snippet: Double immunofluorescence staining of NLRC5 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRC5 (red) and CD31 (green). b Co-localization of NLRC5 (red) and integrin-α (green). c Co-localization of NLRC5 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right

Article Snippet: Glomerular endothelial cells, mesangial cells and podocytes were identified using immunofluorescence staining with primary antibodies against CD31 (1:50, sc53411; Santa Cruz Biotechnology, CA), integrin-α8 (1:50, sc365798; Santa Cruz Biotechnology, CA) and synaptopodin (1:50, MAB8977; R&D Systems, Minneapolis, MN, USA) respectively.

Techniques: Double Immunofluorescence Staining