Journal: Bioengineering & Translational Medicine
Article Title: Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes
Figure Lengend Snippet: Mutual interactions among cells within 3D spheroids enhance cellular functionality. Expression levels of mouse (a) Synpo and (b) Vegfa and human (c) VEGFA and (d) HGF mRNA in hybrid 3D cell spheroids determined by real‐time quantitative PCR. The results are expressed as the fold change relative to the control podocytes ( n = 3). (e) Western blot for laminin, fibronectin (FN) and β‐actin in hybrid 3D cell spheroids and the corresponding quantitative results normalized to β‐actin ( n = 3). Data are presented as mean ± SD. All p values were calculated using one‐way ANOVA with the Tukey correction. * p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001; ns, not significant
Article Snippet: Relative gene expression was determined using TaqMan gene expression assays for mouse Gapdh (Mm99999915_g1), Synpo (Mm03413333_m1), and Vegfa (Mm00437304_m1) and human GAPDH (Hs99999905_m1), VEGFA (Hs00900054_m1), and HGF (Hs00300159_m1) and the TaqMan Universal Master Mix II on a StepOnePlus Real‐Time PCR System (Thermo Fisher Scientific); each analysis was performed in triplicate.
Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot