Journal: PLoS ONE

Article Title: Differentiation of human iPSCs into functional podocytes

doi: 10.1371/journal.pone.0203869

Figure Lengend Snippet: The pluripotency markers Oct4 and Sox-2 gradually decreased to zero at day 13, while podocyte marker synaptopodin (SYNPO) mRNA specifically increased 9-fold during the course of differentiation. Data are given as means ± SEM of 7 independent series of experiments, pooled from the 3 iPSC lines (SBAD3, SBNEO, SFC018) (*P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: Assays for synaptopodin (SYNPO, Hs00702468_s1), Oct4 (Hs04260367_gH) and Sox-2 (Hs01053049_s1) were purchased (TaqMan Gene Expression Assays, Life Technologies), based on the supplier’s validated bioinformatics pipeline for optimal alignment to the selected genes.

Techniques: Marker

Journal: PLoS ONE

Article Title: Differentiation of human iPSCs into functional podocytes

doi: 10.1371/journal.pone.0203869

Figure Lengend Snippet: The podocyte cell line (Saleem et al., 2002) was cultured under non-permissive conditions. Phase contrast images of cell morphology (left) and synaptopodin immunofluorescence staining (middle). Right: Staining of cell nucleus with Hoechst 33342.

Article Snippet: Assays for synaptopodin (SYNPO, Hs00702468_s1), Oct4 (Hs04260367_gH) and Sox-2 (Hs01053049_s1) were purchased (TaqMan Gene Expression Assays, Life Technologies), based on the supplier’s validated bioinformatics pipeline for optimal alignment to the selected genes.

Techniques: Cell Culture, Immunofluorescence, Staining

Journal: PLoS ONE

Article Title: Differentiation of human iPSCs into functional podocytes

doi: 10.1371/journal.pone.0203869

Figure Lengend Snippet: iPSC were differentiated on glass cover slips fixed and stained for synaptopodin, WT-1, podocin, and F-actin as described in methods. Red and green colours were applied post capture. Staining in two other iPSC lines are provided in and Figs.

Article Snippet: Assays for synaptopodin (SYNPO, Hs00702468_s1), Oct4 (Hs04260367_gH) and Sox-2 (Hs01053049_s1) were purchased (TaqMan Gene Expression Assays, Life Technologies), based on the supplier’s validated bioinformatics pipeline for optimal alignment to the selected genes.

Techniques: Staining

Journal: Bioengineering & Translational Medicine

Article Title: Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes

doi: 10.1002/btm2.10212

Figure Lengend Snippet: Mutual interactions among cells within 3D spheroids enhance cellular functionality. Expression levels of mouse (a) Synpo and (b) Vegfa and human (c) VEGFA and (d) HGF mRNA in hybrid 3D cell spheroids determined by real‐time quantitative PCR. The results are expressed as the fold change relative to the control podocytes ( n = 3). (e) Western blot for laminin, fibronectin (FN) and β‐actin in hybrid 3D cell spheroids and the corresponding quantitative results normalized to β‐actin ( n = 3). Data are presented as mean ± SD. All p values were calculated using one‐way ANOVA with the Tukey correction. * p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001; ns, not significant

Article Snippet: Relative gene expression was determined using TaqMan gene expression assays for mouse Gapdh (Mm99999915_g1), Synpo (Mm03413333_m1), and Vegfa (Mm00437304_m1) and human GAPDH (Hs99999905_m1), VEGFA (Hs00900054_m1), and HGF (Hs00300159_m1) and the TaqMan Universal Master Mix II on a StepOnePlus Real‐Time PCR System (Thermo Fisher Scientific); each analysis was performed in triplicate.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/ β -Catenin Signaling Activation in Podocytes

doi: 10.1155/2015/827472

Figure Lengend Snippet: Cultured conditionally immortalized mouse podocytes in undifferentiated and differentiated statuses. (a) and (b) Morphology of cultured podocytes in undifferentiated status (left) and differentiated status (enlarged cell bodies with short and long projections, right) (phase microscopy ×200). (c) and (d) Diffuse cytoplasmic expression of nephrin in undifferentiated status (left); cell surface and cytoplasmic expression of nephrin in differentiated status (right) (immunofluorescence microscopy ×1000). (e) and (f) No expression of synaptopodin in undifferentiated status (left); filamentous and cell surface expression of synaptopodin in differentiated status (right) (immunofluorescence microscopy ×1000).

Article Snippet: Fixed podocytes were incubated overnight at 4°C with either rabbit anti-nephrin antibodies (1 : 100, Abcam) or mouse anti-synaptopodin antibodies (1 : 100, Acris GmbH).

Techniques: Cell Culture, Microscopy, Expressing, Immunofluorescence