sybr stain Lonza Search Results


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  • 97
    Lonza nucleic acid gel stain
    Nucleic Acid Gel Stain, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleic acid gel stain/product/Lonza
    Average 97 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    nucleic acid gel stain - by Bioz Stars, 2020-08
    97/100 stars
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    93
    Lonza sybr gel stain
    Sybr Gel Stain, supplied by Lonza, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr gel stain/product/Lonza
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    sybr gel stain - by Bioz Stars, 2020-08
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    97
    Lonza sybr green nucleic acid stain
    Isolation and characterization of EL cccDNA A) Episomal cccDNA was purified from EL cells by alkaline lysis and CsCl-EtBr gradient centrifugation. Density of gradient fractions decreases from left to right. Fractions were analyzed by agarose gel electrophoresis followed by <t>SYBRGreen®</t> staining. Figure is a representative of 7 independent experiments. *High molecular weight genomic DNA; **genomic broken linear DNA front. B) SYBRGreen™ staining of purified total cccDNA fractions from EL and normal T-cells. C) Southern Blotting of DNA (from B) using a probe of radiolabeled human mtDNA-specific oligonucleotides. D) Electron Microscopy of EL cccDNA. The contour length of the concatemeric mtDNA was measured using the Image Processing and Analysis In Java (ImageJ) software from the NIH. DNA contour length was calculated to be around 10.3 μm. Similar results were obtained measuring the contour length of 50 other molecules. E) Banding pattern of fully digested EL cccDNA with BamHI (10U) (mtDNA single cutter) at 30, 60 and 90 min resembles that of linear human mtDNA monomer (16.5 kbp). F) Partial digestion of EL cccDNA with BamHI (0.1U) stopped at increasing time points (5s, 10s, 20s, 40s, 1min, 2min, 5min, 10min, 20min, 40 min, 1hr). After 5 minutes, two bands of linear DNA are observed representing putative human mtDNA monomers and dimers (16.5 and 33 kbp respectively).
    Sybr Green Nucleic Acid Stain, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green nucleic acid stain/product/Lonza
    Average 97 stars, based on 155 article reviews
    Price from $9.99 to $1999.99
    sybr green nucleic acid stain - by Bioz Stars, 2020-08
    97/100 stars
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    91
    Lonza sybr stain
    Isolation and characterization of EL cccDNA A) Episomal cccDNA was purified from EL cells by alkaline lysis and CsCl-EtBr gradient centrifugation. Density of gradient fractions decreases from left to right. Fractions were analyzed by agarose gel electrophoresis followed by <t>SYBRGreen®</t> staining. Figure is a representative of 7 independent experiments. *High molecular weight genomic DNA; **genomic broken linear DNA front. B) SYBRGreen™ staining of purified total cccDNA fractions from EL and normal T-cells. C) Southern Blotting of DNA (from B) using a probe of radiolabeled human mtDNA-specific oligonucleotides. D) Electron Microscopy of EL cccDNA. The contour length of the concatemeric mtDNA was measured using the Image Processing and Analysis In Java (ImageJ) software from the NIH. DNA contour length was calculated to be around 10.3 μm. Similar results were obtained measuring the contour length of 50 other molecules. E) Banding pattern of fully digested EL cccDNA with BamHI (10U) (mtDNA single cutter) at 30, 60 and 90 min resembles that of linear human mtDNA monomer (16.5 kbp). F) Partial digestion of EL cccDNA with BamHI (0.1U) stopped at increasing time points (5s, 10s, 20s, 40s, 1min, 2min, 5min, 10min, 20min, 40 min, 1hr). After 5 minutes, two bands of linear DNA are observed representing putative human mtDNA monomers and dimers (16.5 and 33 kbp respectively).
    Sybr Stain, supplied by Lonza, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr stain/product/Lonza
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    sybr stain - by Bioz Stars, 2020-08
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    94
    Lonza sybr green ii rna gel stain
    Probing <t>RNA</t> in liposomes with <t>SYBR.</t> ( A ); and measured by FCM. The horizontal axis shows SYBR fluorescence
    Sybr Green Ii Rna Gel Stain, supplied by Lonza, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green ii rna gel stain/product/Lonza
    Average 94 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    sybr green ii rna gel stain - by Bioz Stars, 2020-08
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    89
    Lonza sybr green staining
    Probing <t>RNA</t> in liposomes with <t>SYBR.</t> ( A ); and measured by FCM. The horizontal axis shows SYBR fluorescence
    Sybr Green Staining, supplied by Lonza, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green staining/product/Lonza
    Average 89 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    sybr green staining - by Bioz Stars, 2020-08
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    88
    Lonza sybr green gel staining solution
    Probing <t>RNA</t> in liposomes with <t>SYBR.</t> ( A ); and measured by FCM. The horizontal axis shows SYBR fluorescence
    Sybr Green Gel Staining Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green gel staining solution/product/Lonza
    Average 88 stars, based on 6 article reviews
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    92
    Lonza 1×sybr green
    Probing <t>RNA</t> in liposomes with <t>SYBR.</t> ( A ); and measured by FCM. The horizontal axis shows SYBR fluorescence
    1×Sybr Green, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1×sybr green/product/Lonza
    Average 92 stars, based on 12 article reviews
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    1×sybr green - by Bioz Stars, 2020-08
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    99
    Lonza metaphor agarose
    Probing <t>RNA</t> in liposomes with <t>SYBR.</t> ( A ); and measured by FCM. The horizontal axis shows SYBR fluorescence
    Metaphor Agarose, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/metaphor agarose/product/Lonza
    Average 99 stars, based on 467 article reviews
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    92
    Lonza sybr green ii
    Synthesis of circular RNAs. ( A ) A scheme for the synthesis of circular RNAs used in this study. Transcribed linear RNAs were annealed to its complementary DNA oligomer and then ligated using T4 RNA ligase 2 to produce the circular RNA. ( B , C ) Verification of their circularity of the RNAs. The RNAs were incubated with RNase R and the reactions were analysed by denaturing PAGE. The gels were <t>visualised</t> by <t>SYBR</t> Green II staining.
    Sybr Green Ii, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green ii/product/Lonza
    Average 92 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    sybr green ii - by Bioz Stars, 2020-08
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    Image Search Results


    Isolation and characterization of EL cccDNA A) Episomal cccDNA was purified from EL cells by alkaline lysis and CsCl-EtBr gradient centrifugation. Density of gradient fractions decreases from left to right. Fractions were analyzed by agarose gel electrophoresis followed by SYBRGreen® staining. Figure is a representative of 7 independent experiments. *High molecular weight genomic DNA; **genomic broken linear DNA front. B) SYBRGreen™ staining of purified total cccDNA fractions from EL and normal T-cells. C) Southern Blotting of DNA (from B) using a probe of radiolabeled human mtDNA-specific oligonucleotides. D) Electron Microscopy of EL cccDNA. The contour length of the concatemeric mtDNA was measured using the Image Processing and Analysis In Java (ImageJ) software from the NIH. DNA contour length was calculated to be around 10.3 μm. Similar results were obtained measuring the contour length of 50 other molecules. E) Banding pattern of fully digested EL cccDNA with BamHI (10U) (mtDNA single cutter) at 30, 60 and 90 min resembles that of linear human mtDNA monomer (16.5 kbp). F) Partial digestion of EL cccDNA with BamHI (0.1U) stopped at increasing time points (5s, 10s, 20s, 40s, 1min, 2min, 5min, 10min, 20min, 40 min, 1hr). After 5 minutes, two bands of linear DNA are observed representing putative human mtDNA monomers and dimers (16.5 and 33 kbp respectively).

    Journal: Leukemia research

    Article Title: Identification of Mitochondrial Genome Concatemers in AIDS-associated lymphomas and lymphoid cell lines

    doi: 10.1016/j.leukres.2009.03.012

    Figure Lengend Snippet: Isolation and characterization of EL cccDNA A) Episomal cccDNA was purified from EL cells by alkaline lysis and CsCl-EtBr gradient centrifugation. Density of gradient fractions decreases from left to right. Fractions were analyzed by agarose gel electrophoresis followed by SYBRGreen® staining. Figure is a representative of 7 independent experiments. *High molecular weight genomic DNA; **genomic broken linear DNA front. B) SYBRGreen™ staining of purified total cccDNA fractions from EL and normal T-cells. C) Southern Blotting of DNA (from B) using a probe of radiolabeled human mtDNA-specific oligonucleotides. D) Electron Microscopy of EL cccDNA. The contour length of the concatemeric mtDNA was measured using the Image Processing and Analysis In Java (ImageJ) software from the NIH. DNA contour length was calculated to be around 10.3 μm. Similar results were obtained measuring the contour length of 50 other molecules. E) Banding pattern of fully digested EL cccDNA with BamHI (10U) (mtDNA single cutter) at 30, 60 and 90 min resembles that of linear human mtDNA monomer (16.5 kbp). F) Partial digestion of EL cccDNA with BamHI (0.1U) stopped at increasing time points (5s, 10s, 20s, 40s, 1min, 2min, 5min, 10min, 20min, 40 min, 1hr). After 5 minutes, two bands of linear DNA are observed representing putative human mtDNA monomers and dimers (16.5 and 33 kbp respectively).

    Article Snippet: The gel was stained with SYBRGreen® Nucleic Acid Stain (Lonza Rockland, Inc., Rockland, ME) for 1 hr, de-stained in 10mM Tris 1mM EDTA for 30 minutes and visualized using a Molecular Dynamics STORM Phosphor Imager (Model 860-PC, Amersham Biosciences, Piscataway, NJ).

    Techniques: Isolation, Purification, Alkaline Lysis, Gradient Centrifugation, Agarose Gel Electrophoresis, Staining, Molecular Weight, Southern Blot, Electron Microscopy, Software

    Schematic representation of the Gardella technique and visualization of circular episomal DNA in AIDS-associated lymphoma EL cells A) One million live cells in 10% Ficoll buffer are loaded into a well of a vertical agarose gel and digested by overlaying with a lysis buffer containing SDS and pronase and electrophoresis at 22 V for 3 hours. After electrophoresis at 70 volts for 18 h, the gel is stained and the DNA is visualized by staining with Sybr Green. The three different molecular configuration of DNA is indicated. B) SYBRGreen™ staining of Gardella gel of the unknown episomal DNA from EL cells (arrow) migrating within the range of large cccDNA.

    Journal: Leukemia research

    Article Title: Identification of Mitochondrial Genome Concatemers in AIDS-associated lymphomas and lymphoid cell lines

    doi: 10.1016/j.leukres.2009.03.012

    Figure Lengend Snippet: Schematic representation of the Gardella technique and visualization of circular episomal DNA in AIDS-associated lymphoma EL cells A) One million live cells in 10% Ficoll buffer are loaded into a well of a vertical agarose gel and digested by overlaying with a lysis buffer containing SDS and pronase and electrophoresis at 22 V for 3 hours. After electrophoresis at 70 volts for 18 h, the gel is stained and the DNA is visualized by staining with Sybr Green. The three different molecular configuration of DNA is indicated. B) SYBRGreen™ staining of Gardella gel of the unknown episomal DNA from EL cells (arrow) migrating within the range of large cccDNA.

    Article Snippet: The gel was stained with SYBRGreen® Nucleic Acid Stain (Lonza Rockland, Inc., Rockland, ME) for 1 hr, de-stained in 10mM Tris 1mM EDTA for 30 minutes and visualized using a Molecular Dynamics STORM Phosphor Imager (Model 860-PC, Amersham Biosciences, Piscataway, NJ).

    Techniques: Agarose Gel Electrophoresis, Lysis, Electrophoresis, Staining, SYBR Green Assay

    Allele-specific amplification and detection of the PCR products . ( A ) Schematic representation of the allele-specific primer PCR method. 'Koshihikari' (Ksh) allele specific primer forms a perfect match at the 3' end (SNP) with Ksh DNA sequence (1) but forms a mismatch with Kal DNA (2). 'Kasalath' (Kal) allele specific primer similarly forms a 3' end match with Kal DNA (4) and 3' end mismatch with Ksh DNA (3). Both allele specific primer has an artificial mismatch at third base from 3' end (blue circle) according to the result from Table 1. ( B ) Allele-specific amplification of SNP marker S0285 detected EtBr after gel electrophoresis. Fluorescence of same samples were detected with a UV transilluminator at room temperature (25°C) or immediately after heating to 80°C using SYBR Green I. Ksh allele-specific primers (lane 1 and 2) and Kal allele-specific primers (lane 3 and 4) were used for PCR of Ksh genomic DNA (lane 1 and 3) and Kal genomic DNA (lane 2 and 4). The specificity of the reaction is evident. ( C ) The effect of temperature on SYBR Green I fluorescence. The green line indicates the fluorescence intensity of the sample in which amplification had occurred, and the blue line shows the amplification-independent background fluorescence. The ratio of both (signal/noise ratio) is shown in red. ( D ) The relationship between the number of SNPs and samples in a single PCR operation using 96-well plate. In a single PCR operation, 48 samples SNP genotype could be examined (multi-sample, Fig. 2), or 48 locus SNPs of one sample could be examined (multi-locus, Fig. 3)

    Journal: Plant Methods

    Article Title: Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species

    doi: 10.1186/1746-4811-6-12

    Figure Lengend Snippet: Allele-specific amplification and detection of the PCR products . ( A ) Schematic representation of the allele-specific primer PCR method. 'Koshihikari' (Ksh) allele specific primer forms a perfect match at the 3' end (SNP) with Ksh DNA sequence (1) but forms a mismatch with Kal DNA (2). 'Kasalath' (Kal) allele specific primer similarly forms a 3' end match with Kal DNA (4) and 3' end mismatch with Ksh DNA (3). Both allele specific primer has an artificial mismatch at third base from 3' end (blue circle) according to the result from Table 1. ( B ) Allele-specific amplification of SNP marker S0285 detected EtBr after gel electrophoresis. Fluorescence of same samples were detected with a UV transilluminator at room temperature (25°C) or immediately after heating to 80°C using SYBR Green I. Ksh allele-specific primers (lane 1 and 2) and Kal allele-specific primers (lane 3 and 4) were used for PCR of Ksh genomic DNA (lane 1 and 3) and Kal genomic DNA (lane 2 and 4). The specificity of the reaction is evident. ( C ) The effect of temperature on SYBR Green I fluorescence. The green line indicates the fluorescence intensity of the sample in which amplification had occurred, and the blue line shows the amplification-independent background fluorescence. The ratio of both (signal/noise ratio) is shown in red. ( D ) The relationship between the number of SNPs and samples in a single PCR operation using 96-well plate. In a single PCR operation, 48 samples SNP genotype could be examined (multi-sample, Fig. 2), or 48 locus SNPs of one sample could be examined (multi-locus, Fig. 3)

    Article Snippet: Add 2 μl 10 × SYBR Green I (catalogue No. 50513, Lonza, Basel, Switzerland) to the PCR product, and detect the fluorescence at 75°C.

    Techniques: Amplification, Polymerase Chain Reaction, Sequencing, Marker, Nucleic Acid Electrophoresis, Fluorescence, SYBR Green Assay

    Optimization of the best condition of SYBR Green I (SG I) staining for fluorescence assay in mice using different concentrations of the stain. ( a ) 0.25x SG I; ( b ) 0.5x SG I; ( c ) 1x SG I; ( d ) 2x SG I; ( e ) 4x SG I; ( f ) 8x SG I. Each value represents the mean of duplicate trails of 5 mice per experimental group after subtracting the background fluorescence of non-parasitized RBCs from uninfected mice. Gain values are not set to 100.

    Journal: Scientific Reports

    Article Title: Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice

    doi: 10.1038/s41598-017-13052-5

    Figure Lengend Snippet: Optimization of the best condition of SYBR Green I (SG I) staining for fluorescence assay in mice using different concentrations of the stain. ( a ) 0.25x SG I; ( b ) 0.5x SG I; ( c ) 1x SG I; ( d ) 2x SG I; ( e ) 4x SG I; ( f ) 8x SG I. Each value represents the mean of duplicate trails of 5 mice per experimental group after subtracting the background fluorescence of non-parasitized RBCs from uninfected mice. Gain values are not set to 100.

    Article Snippet: Chemical reagents SYBR Green I (SG I) nucleic acid stain (Lonza, USA; 10,000x) was stored at −20 °C and thawed before use.

    Techniques: SYBR Green Assay, Staining, Fluorescence, Mouse Assay

    Comparison of the emitted fluorescence signals and parasitemia detected by fluorescence- and microscopy-based methods, respectively, using different concentrations of SYBR Green I stain. ( a ) Microscopy-based method; ( b ) fluorescence-based method using 1x SG I stain; ( c ) fluorescence-based method using 2x SG I stain; ( d ) fluorescence-based method using 4x SG I stain; ( e ) fluorescence-based method using 8x SG I stain. Each value represents the mean ± standard deviation of 5 mice per experimental group after subtracting the background fluorescence for non-parasitized RBCs from uninfected mice. Gain values are not set to 100.

    Journal: Scientific Reports

    Article Title: Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice

    doi: 10.1038/s41598-017-13052-5

    Figure Lengend Snippet: Comparison of the emitted fluorescence signals and parasitemia detected by fluorescence- and microscopy-based methods, respectively, using different concentrations of SYBR Green I stain. ( a ) Microscopy-based method; ( b ) fluorescence-based method using 1x SG I stain; ( c ) fluorescence-based method using 2x SG I stain; ( d ) fluorescence-based method using 4x SG I stain; ( e ) fluorescence-based method using 8x SG I stain. Each value represents the mean ± standard deviation of 5 mice per experimental group after subtracting the background fluorescence for non-parasitized RBCs from uninfected mice. Gain values are not set to 100.

    Article Snippet: Chemical reagents SYBR Green I (SG I) nucleic acid stain (Lonza, USA; 10,000x) was stored at −20 °C and thawed before use.

    Techniques: Fluorescence, Microscopy, SYBR Green Assay, Staining, Standard Deviation, Mouse Assay

    Probing RNA in liposomes with SYBR. ( A ); and measured by FCM. The horizontal axis shows SYBR fluorescence

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Sustainable proliferation of liposomes compatible with inner RNA replication

    doi: 10.1073/pnas.1516893113

    Figure Lengend Snippet: Probing RNA in liposomes with SYBR. ( A ); and measured by FCM. The horizontal axis shows SYBR fluorescence

    Article Snippet: SYBR Green II RNA Gel Stain was purchased from Lonza.

    Techniques: Fluorescence

    Synthesis of circular RNAs. ( A ) A scheme for the synthesis of circular RNAs used in this study. Transcribed linear RNAs were annealed to its complementary DNA oligomer and then ligated using T4 RNA ligase 2 to produce the circular RNA. ( B , C ) Verification of their circularity of the RNAs. The RNAs were incubated with RNase R and the reactions were analysed by denaturing PAGE. The gels were visualised by SYBR Green II staining.

    Journal: Scientific Reports

    Article Title: Rolling Circle Translation of Circular RNA in Living Human Cells

    doi: 10.1038/srep16435

    Figure Lengend Snippet: Synthesis of circular RNAs. ( A ) A scheme for the synthesis of circular RNAs used in this study. Transcribed linear RNAs were annealed to its complementary DNA oligomer and then ligated using T4 RNA ligase 2 to produce the circular RNA. ( B , C ) Verification of their circularity of the RNAs. The RNAs were incubated with RNase R and the reactions were analysed by denaturing PAGE. The gels were visualised by SYBR Green II staining.

    Article Snippet: The gels were electrophoresed and stained with SYBR Green II (Lonza) and visualised on a BioRad ChemiDoc XRS+ System (BioRad, Hercules, CA, USA), Luminescent Image Analyser LAS 4000 (Fujifilm, Tokyo, Japan) or FAS-IV Imaging System (Nippon Genetics, Tokyo, Japan).

    Techniques: Incubation, Polyacrylamide Gel Electrophoresis, SYBR Green Assay, Staining