sybr select master mix Thermo Fisher Search Results


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  • 94
    Thermo Fisher sybr master mixture
    Sybr Master Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr select master mix
    Sybr Select Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7803 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr select master mix kit
    Sybr Select Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fast sybr green master mix
    Fast Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green select master mix
    Sybr Green Select Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr select qpcr master mix
    Sybr Select Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher maxima sybr green master mix
    Maxima Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher powerup sybr green master mix
    Powerup Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green selected master mix
    Sybr Green Selected Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher maxima sybr green fluorescein qpcr master mix
    Maxima Sybr Green Fluorescein Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher amplitaq gold pcr master mix
    Use of exo+ and exo− Taq polymerase. Eprobe mediated real-time <t>PCR</t> experiments were performed by using an exo+ <t>(Amplitaq)</t> and exo− (Genotyping Master) Taq polymerase and Eprobes with different melting temperatures. Amplification curves (Random fluorescent units (RFU) plotted against PCR cycle number) using a 7 times serial dilution of the DNA template are shown on the left. The R-squared values of the PCR efficiency plots are indicated in the graphs. Differential melting curve analysis by plotting –dF/dT against temperature is shown on the right. Main peaks are indicated in the graph to show the different T M values for both Eprobes. EGFR wild-type plasmid DNA concentrations are indicated by colors: Red: 1.5×10 8 copies, Dark blue: 1.5×10 7 copies, Yellow: 1.5×10 6 copies, Green: 1.5×10 5 copies, Pink: 1.5×10 4 copies, Sky blue: 1.5×10 3 copies, Brown: 150 copies, Orange: TE negative control. A: Amplitaq and Eprobe 215-21 wt TO. B: Genotyping Mastermix and Eprobe 215-21 TO. C: Amplitaq and Eprobe 205-13 wt TO. D: Genotyping Master and Eprobe 205-13 wt TO.
    Amplitaq Gold Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1050 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mirnas taqman universal pcr master mix
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Mirnas Taqman Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr� select pcr master mix
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Sybr� Select Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher power sybr green pcr master mix
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr select pcr master mix
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Sybr Select Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green select qpcr supermix
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Sybr Green Select Qpcr Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum sybr green qpcr supermix udg
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Platinum Sybr Green Qpcr Supermix Udg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher revertaid first strand cdna synthesis kit
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Revertaid First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher powersybr green cells to ct kit
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Powersybr Green Cells To Ct Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher power sybr green rna to ct 1 step qrt pcr assay
    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via <t>qRT-PCR</t> for <t>miRNAs</t> of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p
    Power Sybr Green Rna To Ct 1 Step Qrt Pcr Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Use of exo+ and exo− Taq polymerase. Eprobe mediated real-time PCR experiments were performed by using an exo+ (Amplitaq) and exo− (Genotyping Master) Taq polymerase and Eprobes with different melting temperatures. Amplification curves (Random fluorescent units (RFU) plotted against PCR cycle number) using a 7 times serial dilution of the DNA template are shown on the left. The R-squared values of the PCR efficiency plots are indicated in the graphs. Differential melting curve analysis by plotting –dF/dT against temperature is shown on the right. Main peaks are indicated in the graph to show the different T M values for both Eprobes. EGFR wild-type plasmid DNA concentrations are indicated by colors: Red: 1.5×10 8 copies, Dark blue: 1.5×10 7 copies, Yellow: 1.5×10 6 copies, Green: 1.5×10 5 copies, Pink: 1.5×10 4 copies, Sky blue: 1.5×10 3 copies, Brown: 150 copies, Orange: TE negative control. A: Amplitaq and Eprobe 215-21 wt TO. B: Genotyping Mastermix and Eprobe 215-21 TO. C: Amplitaq and Eprobe 205-13 wt TO. D: Genotyping Master and Eprobe 205-13 wt TO.

    Journal: PLoS ONE

    Article Title: Eprobe Mediated Real-Time PCR Monitoring and Melting Curve Analysis

    doi: 10.1371/journal.pone.0070942

    Figure Lengend Snippet: Use of exo+ and exo− Taq polymerase. Eprobe mediated real-time PCR experiments were performed by using an exo+ (Amplitaq) and exo− (Genotyping Master) Taq polymerase and Eprobes with different melting temperatures. Amplification curves (Random fluorescent units (RFU) plotted against PCR cycle number) using a 7 times serial dilution of the DNA template are shown on the left. The R-squared values of the PCR efficiency plots are indicated in the graphs. Differential melting curve analysis by plotting –dF/dT against temperature is shown on the right. Main peaks are indicated in the graph to show the different T M values for both Eprobes. EGFR wild-type plasmid DNA concentrations are indicated by colors: Red: 1.5×10 8 copies, Dark blue: 1.5×10 7 copies, Yellow: 1.5×10 6 copies, Green: 1.5×10 5 copies, Pink: 1.5×10 4 copies, Sky blue: 1.5×10 3 copies, Brown: 150 copies, Orange: TE negative control. A: Amplitaq and Eprobe 215-21 wt TO. B: Genotyping Mastermix and Eprobe 215-21 TO. C: Amplitaq and Eprobe 205-13 wt TO. D: Genotyping Master and Eprobe 205-13 wt TO.

    Article Snippet: We selected AmpliTaq Gold PCR Master Mix (Applied Biosystems, Carlsbad, USA) as a Taq DNA polymerase with an exonuclease activity (exo+) and LightCycler 480 Genotyping Master (Roche Diagnostics, Mannheim, Germany) as a Taq DNA polymerase having an N-terminal deletion lacking an exonuclease activity (exo−).

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Serial Dilution, Plasmid Preparation, Negative Control

    Eprobe mediated real-time PCR monitoring. Real-time PCR experiments were performed using different Eprobes, a TaqMan probe, SYBR Green I, Amplitaq and different concentrations of an EGFR wild-type plasmid DNA template. Amplification curves (Random fluorescent units (RFU) plotted against PCR cycle number) using a 7 times serial dilution of the DNA template are shown on the left, and PCR efficiency plots (Ct values plotted against logarithm of plasmid DNA concentrations) are shown on the right. Plasmid DNA concentrations are indicated by colors: Red: 1.5×10 8 copies, Dark blue: 1.5×10 7 copies, Yellow: 1.5×10 6 copies, Green: 1.5×10 5 copies, Pink: 1.5×10 4 copies, Sky blue: 1.5×10 3 copies, Brown: 150 copies, Orange: TE negative control. A: Eprobe 203-10 wt TO, B: Eprobe 205-13 wt TO, C: Eprobe 215-21 wt TO, D: Eprobe 215-21 wt TP, E: SYBR Green I, F: TaqMan probe. Triplicate data are shown for each experiment. For Eprobe 203-10 wt early Ct values for the highest template concentration were not recorded by the PCR machine.

    Journal: PLoS ONE

    Article Title: Eprobe Mediated Real-Time PCR Monitoring and Melting Curve Analysis

    doi: 10.1371/journal.pone.0070942

    Figure Lengend Snippet: Eprobe mediated real-time PCR monitoring. Real-time PCR experiments were performed using different Eprobes, a TaqMan probe, SYBR Green I, Amplitaq and different concentrations of an EGFR wild-type plasmid DNA template. Amplification curves (Random fluorescent units (RFU) plotted against PCR cycle number) using a 7 times serial dilution of the DNA template are shown on the left, and PCR efficiency plots (Ct values plotted against logarithm of plasmid DNA concentrations) are shown on the right. Plasmid DNA concentrations are indicated by colors: Red: 1.5×10 8 copies, Dark blue: 1.5×10 7 copies, Yellow: 1.5×10 6 copies, Green: 1.5×10 5 copies, Pink: 1.5×10 4 copies, Sky blue: 1.5×10 3 copies, Brown: 150 copies, Orange: TE negative control. A: Eprobe 203-10 wt TO, B: Eprobe 205-13 wt TO, C: Eprobe 215-21 wt TO, D: Eprobe 215-21 wt TP, E: SYBR Green I, F: TaqMan probe. Triplicate data are shown for each experiment. For Eprobe 203-10 wt early Ct values for the highest template concentration were not recorded by the PCR machine.

    Article Snippet: We selected AmpliTaq Gold PCR Master Mix (Applied Biosystems, Carlsbad, USA) as a Taq DNA polymerase with an exonuclease activity (exo+) and LightCycler 480 Genotyping Master (Roche Diagnostics, Mannheim, Germany) as a Taq DNA polymerase having an N-terminal deletion lacking an exonuclease activity (exo−).

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Plasmid Preparation, Amplification, Polymerase Chain Reaction, Serial Dilution, Negative Control, Concentration Assay

    miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via qRT-PCR for miRNAs of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p

    Journal: Scientific Reports

    Article Title: Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma

    doi: 10.1038/srep46026

    Figure Lengend Snippet: miRNA regulation in murine allergic airway inflammation. ( a ) Expression analysis via qRT-PCR for miRNAs of lung homogenates of PBS/OVA and OVA/OVA mice (n = 5 mice per group), y-axis depicts dCp values (Cp miRNA -Cp reference miR ) – higher dCp means lower expression (reversed scale), Mann-Whitney U, *p

    Article Snippet: PCRs were performed on the LightCycler 480 platform (Roche, Mannheim, Germany) using LightCycler 480 SYBR Green I Mastermix or for miRNAs TaqMan® Universal PCR Master Mix (Invitrogen, Carlsbad, USA).

    Techniques: Expressing, Quantitative RT-PCR, Mouse Assay, MANN-WHITNEY

    miRNA-regulation of CREB1 in vitro. Transfection of 16-HBE14o − cells with (a) PremiRs or scrambled miRNA and a luciferase-vector containing the Creb1 3′-UTR fused to Renilla luciferase, representative of two independent experiments with n = 3 wells. (b,c) AntimiR transfection (n = 3). (b) qRT-PCR for miRNAs and (c) Creb1. All mean ± SD, *p

    Journal: Scientific Reports

    Article Title: Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma

    doi: 10.1038/srep46026

    Figure Lengend Snippet: miRNA-regulation of CREB1 in vitro. Transfection of 16-HBE14o − cells with (a) PremiRs or scrambled miRNA and a luciferase-vector containing the Creb1 3′-UTR fused to Renilla luciferase, representative of two independent experiments with n = 3 wells. (b,c) AntimiR transfection (n = 3). (b) qRT-PCR for miRNAs and (c) Creb1. All mean ± SD, *p

    Article Snippet: PCRs were performed on the LightCycler 480 platform (Roche, Mannheim, Germany) using LightCycler 480 SYBR Green I Mastermix or for miRNAs TaqMan® Universal PCR Master Mix (Invitrogen, Carlsbad, USA).

    Techniques: In Vitro, Transfection, Luciferase, Plasmid Preparation, Quantitative RT-PCR

    Pulmonary miRNA profile and selection of Creb1 as target gene. ( a ) Fold changes of miRNA expression analyzed by Exiqon microarray, TaqMan ® microarray and qRT-PCR of lung homogenate of mice with OVA-induced allergic airway inflammation and healthy controls on d72 of the treatment protocol (n = 5 mice per group) with adjusted p-value. n.d.: not detected; - miRNA not available on the array; *minor strand of miRNA. ( b) List of genes and miRNA-binding sites in their 3′-UTRs as predicted by five different algorithms (miRanda, PicTar, PITA, TargetSpy TargetScanS). Darker color depicts more binding sites for the same miRNA.

    Journal: Scientific Reports

    Article Title: Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma

    doi: 10.1038/srep46026

    Figure Lengend Snippet: Pulmonary miRNA profile and selection of Creb1 as target gene. ( a ) Fold changes of miRNA expression analyzed by Exiqon microarray, TaqMan ® microarray and qRT-PCR of lung homogenate of mice with OVA-induced allergic airway inflammation and healthy controls on d72 of the treatment protocol (n = 5 mice per group) with adjusted p-value. n.d.: not detected; - miRNA not available on the array; *minor strand of miRNA. ( b) List of genes and miRNA-binding sites in their 3′-UTRs as predicted by five different algorithms (miRanda, PicTar, PITA, TargetSpy TargetScanS). Darker color depicts more binding sites for the same miRNA.

    Article Snippet: PCRs were performed on the LightCycler 480 platform (Roche, Mannheim, Germany) using LightCycler 480 SYBR Green I Mastermix or for miRNAs TaqMan® Universal PCR Master Mix (Invitrogen, Carlsbad, USA).

    Techniques: Selection, Expressing, Microarray, Quantitative RT-PCR, Mouse Assay, Binding Assay