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    TaKaRa sybr premix ex taq
    The expression level of 3D8 scFv protein in recombinant L. reuteri and recombinant L. paracasei ATCC 334 strains. The expression level of 3D8 scFv in transformed L. reuteri was determined with <t>SYBR</t> Premix Ex <t>Taq</t> by real-time RT-PCR
    Sybr Premix Ex Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 55890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa tb green premix ex taq ii
    The expression level of 3D8 scFv protein in recombinant L. reuteri and recombinant L. paracasei ATCC 334 strains. The expression level of 3D8 scFv in transformed L. reuteri was determined with <t>SYBR</t> Premix Ex <t>Taq</t> by real-time RT-PCR
    Tb Green Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tb green premix ex taq ii/product/TaKaRa
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    tb green premix ex taq ii - by Bioz Stars, 2020-09
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    TaKaRa tb green premix ex taq
    The expression level of 3D8 scFv protein in recombinant L. reuteri and recombinant L. paracasei ATCC 334 strains. The expression level of 3D8 scFv in transformed L. reuteri was determined with <t>SYBR</t> Premix Ex <t>Taq</t> by real-time RT-PCR
    Tb Green Premix Ex Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tb green premix ex taq/product/TaKaRa
    Average 99 stars, based on 1046 article reviews
    Price from $9.99 to $1999.99
    tb green premix ex taq - by Bioz Stars, 2020-09
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    The expression level of 3D8 scFv protein in recombinant L. reuteri and recombinant L. paracasei ATCC 334 strains. The expression level of 3D8 scFv in transformed L. reuteri was determined with SYBR Premix Ex Taq by real-time RT-PCR

    Journal: 3 Biotech

    Article Title: Oral administration of Lactobacillus reuteri expressing a 3D8 single-chain variable fragment (scFv) enhances chicken growth and conserves immune homeostasis

    doi: 10.1007/s13205-019-1811-8

    Figure Lengend Snippet: The expression level of 3D8 scFv protein in recombinant L. reuteri and recombinant L. paracasei ATCC 334 strains. The expression level of 3D8 scFv in transformed L. reuteri was determined with SYBR Premix Ex Taq by real-time RT-PCR

    Article Snippet: Quantitative real-time PCR to analyze the expression level of 3D8 scFv in transformed L. reuteri was conducted with SYBR Premix Ex Taq (TaKaRa, Otsu, Shinga, Japan) and a Rotor-Gene Q system (Qiagen, Chadstone, Victoria, Australia).

    Techniques: Expressing, Recombinant, Transformation Assay, Quantitative RT-PCR

    Quantitative analysis of NOX2 and NOX4 mRNA expression in human OS cell lines. Quantitative analysis of the mRNA expression of NOX family proteins in five human OS cell lines was performed by RT-qPCR using a SYBR Green reagent. The Cq value during the exponential phase of amplification was determined by the real-time monitoring of the fluorescent emission of Taq polymerase nuclease activity. β- actin was used as an internal control for normalization. qPCR efficiencies of the target ( NOX2 and NOX4 ) and control (β-actin) proteins were approximately equal over a concentration range of 0.1–200 ng total cDNA. The relative transcripts were determined by the formula: 1/2 (Cq target-Cq control) ). The bars represent the mean (n=2). OS, osteosarcoma; NOX, NADPH oxidase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Cq, quantification cycle; PBMCs, peripheral blood mononuclear cells.

    Journal: Oncology Letters

    Article Title: Inhibition of NADPH oxidase 2 induces apoptosis in osteosarcoma: The role of reactive oxygen species in cell proliferation

    doi: 10.3892/ol.2018.8291

    Figure Lengend Snippet: Quantitative analysis of NOX2 and NOX4 mRNA expression in human OS cell lines. Quantitative analysis of the mRNA expression of NOX family proteins in five human OS cell lines was performed by RT-qPCR using a SYBR Green reagent. The Cq value during the exponential phase of amplification was determined by the real-time monitoring of the fluorescent emission of Taq polymerase nuclease activity. β- actin was used as an internal control for normalization. qPCR efficiencies of the target ( NOX2 and NOX4 ) and control (β-actin) proteins were approximately equal over a concentration range of 0.1–200 ng total cDNA. The relative transcripts were determined by the formula: 1/2 (Cq target-Cq control) ). The bars represent the mean (n=2). OS, osteosarcoma; NOX, NADPH oxidase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Cq, quantification cycle; PBMCs, peripheral blood mononuclear cells.

    Article Snippet: RT-qPCR was performed with SYBR Premix Ex Taq II (Takara Bio, Inc., Otsu, Shiga, Japan) in an ABI PRISM 7500 Sequence Detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay, Amplification, Activity Assay, Real-time Polymerase Chain Reaction, Concentration Assay

    qRT-PCR analysis of the expression of defense signaling genes in transgenic eggplants. Quantitative reverse-transcription PCR (qPCR) was performed using a SYBR Premix Ex Taq kit (TaKaRa, Dalian, China), following the manufacturer’s protocols. Triplicate qPCR reactions were performed for each sample and the relative gene expression data was analyzed using the 2 −ΔΔ Ct method. ( a ) qRT-PCR analysis of defense signaling genes in SmNAC oveexpressing plants. CK represents non-transgenic plants from the E-31 line, whereas 1–3 show the SmNAC overexpressing transgenic T 0 plants EGT 0–87 , EGT 0–145 , and EGT 0–204 , and 4–6 show the SmNAC overexpressing transgenic T 1 plants EGT 1–87 , EGT 1–145 , and EGT 1–204 . ( b ) qRT-PCR analysis of defense signaling genes in RNAi- SmNAC plants. CK represents non-transgenic plants from line E-32. 1–5 show the RNAi- SmNAC transgenic plants (T0) RNAi-1, RNAi-2, RNAi-3, RNAi-4, and RNAi-5.

    Journal: Scientific Reports

    Article Title: Overexpression of the Eggplant (Solanum melongena) NAC Family Transcription Factor SmNAC Suppresses Resistance to Bacterial Wilt

    doi: 10.1038/srep31568

    Figure Lengend Snippet: qRT-PCR analysis of the expression of defense signaling genes in transgenic eggplants. Quantitative reverse-transcription PCR (qPCR) was performed using a SYBR Premix Ex Taq kit (TaKaRa, Dalian, China), following the manufacturer’s protocols. Triplicate qPCR reactions were performed for each sample and the relative gene expression data was analyzed using the 2 −ΔΔ Ct method. ( a ) qRT-PCR analysis of defense signaling genes in SmNAC oveexpressing plants. CK represents non-transgenic plants from the E-31 line, whereas 1–3 show the SmNAC overexpressing transgenic T 0 plants EGT 0–87 , EGT 0–145 , and EGT 0–204 , and 4–6 show the SmNAC overexpressing transgenic T 1 plants EGT 1–87 , EGT 1–145 , and EGT 1–204 . ( b ) qRT-PCR analysis of defense signaling genes in RNAi- SmNAC plants. CK represents non-transgenic plants from line E-32. 1–5 show the RNAi- SmNAC transgenic plants (T0) RNAi-1, RNAi-2, RNAi-3, RNAi-4, and RNAi-5.

    Article Snippet: Quantitative reverse-transcription (qRT-PCR) analysis Quantitative reverse-transcription PCR (qPCR) was performed using gene-specific primers ( ) and a SYBR Premix Ex Taq kit (TaKaRa, Dalian, China), following the manufacturer’s protocols.

    Techniques: Quantitative RT-PCR, Expressing, Transgenic Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Expression levels of miR-192, -194 and -215 in three colorectal cancer (CRC) cell lines (HT-29, HCT-116 and SW-620). Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Data are presented in CRC cell lines relative to normal colorectal tissues

    Journal: Experimental and Therapeutic Medicine

    Article Title: microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

    doi: 10.3892/etm.2011.436

    Figure Lengend Snippet: Expression levels of miR-192, -194 and -215 in three colorectal cancer (CRC) cell lines (HT-29, HCT-116 and SW-620). Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Data are presented in CRC cell lines relative to normal colorectal tissues

    Article Snippet: According to the manufacturer's instructions, real-time PCR was performed using the SYBR Premix Ex Taq™ II kit (Takara Bio, Kyoto, Japan) with a Rotor-gene 6000 system (Qiagen, Valencia, CA, USA) ( ).

    Techniques: Expressing

    Expression levels of miR-192, -194 and -215 in 107 patients with colorectal cancer (CRC). (A, C and E) Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Each sample was analyzed in triplicate and repeated three times. Data are presented

    Journal: Experimental and Therapeutic Medicine

    Article Title: microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

    doi: 10.3892/etm.2011.436

    Figure Lengend Snippet: Expression levels of miR-192, -194 and -215 in 107 patients with colorectal cancer (CRC). (A, C and E) Quantification of miRNAs was measured by SYBR Premix Ex Taq II. Each sample was analyzed in triplicate and repeated three times. Data are presented

    Article Snippet: According to the manufacturer's instructions, real-time PCR was performed using the SYBR Premix Ex Taq™ II kit (Takara Bio, Kyoto, Japan) with a Rotor-gene 6000 system (Qiagen, Valencia, CA, USA) ( ).

    Techniques: Expressing