sybr green reagent Thermo Fisher Search Results


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  • 90
    Thermo Fisher microrna measurements
    In vitro differentiation of bone marrow progenitors from miR-15a-deficient mice A. The levels of miR-15a and miR-150 in LSK cells co cultured with OP9 stromal cells at day 11 measured by TaqMan qPCR; B. Representative flow cytometry analysis of DBA, NZB and DBA−/− LSK derived immature (top box) and mature (bottom box) B cell progenitors at day 11 of OP9 co-culture. C. Quantitative flow cytometry analysis of mature AA4.1 − B220 + (right) and immature (left) B cells progenitors. N = 3, columns represent means and bars are SEMs; Transcript expression analysis of D. PU.1, E. Pax5, and F. cMyb measured by qPCR assay are shown. <t>MicroRNA</t> levels were assessed using TaqMan assays with custom probes for has-miR-15a and has-miR-150. Protein-coding genes transcripts measurements were done by qPCR with the use of SYBR green master mix and specific primers; n = 3, columns represent means and bars are SEMs; G. The ratio of PU.1 to Pax5 gene expression in LSK cells at day 11 of co culture with OP9 cells. Asterisks represent statistically significant difference ( p
    Microrna Measurements, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    microrna measurements - by Bioz Stars, 2020-02
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    99
    Thermo Fisher sybr green reagent
    Real-time <t>RT-PCR</t> . Amplification of TNF- α , MIP-2, IL-6 and GAPDH cDNA in livers from mice that were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg) prior to the onset of ischemia (90 min), followed by 3 h of reperfusion (IR). Results are expressed as fluorescence intensity of <t>SYBR</t> Green bound to DNA (Y-axis). The X-axis represents the number of cycles (i.e. 40 cycles). GAPDH was used as a housekeeping gene. NTC represents PCR products amplified in the absence of cDNA template. Graphs shown are representative of three separate experiments with similar results.
    Sybr Green Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher express sybr green reagent
    Real-time <t>RT-PCR</t> . Amplification of TNF- α , MIP-2, IL-6 and GAPDH cDNA in livers from mice that were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg) prior to the onset of ischemia (90 min), followed by 3 h of reperfusion (IR). Results are expressed as fluorescence intensity of <t>SYBR</t> Green bound to DNA (Y-axis). The X-axis represents the number of cycles (i.e. 40 cycles). GAPDH was used as a housekeeping gene. NTC represents PCR products amplified in the absence of cDNA template. Graphs shown are representative of three separate experiments with similar results.
    Express Sybr Green Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher sybr green core reagents
    Real-time <t>RT-PCR</t> . Amplification of TNF- α , MIP-2, IL-6 and GAPDH cDNA in livers from mice that were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg) prior to the onset of ischemia (90 min), followed by 3 h of reperfusion (IR). Results are expressed as fluorescence intensity of <t>SYBR</t> Green bound to DNA (Y-axis). The X-axis represents the number of cycles (i.e. 40 cycles). GAPDH was used as a housekeeping gene. NTC represents PCR products amplified in the absence of cDNA template. Graphs shown are representative of three separate experiments with similar results.
    Sybr Green Core Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher sybr green core reagents kit
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Sybr Green Core Reagents Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher platinum sybr green
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Platinum Sybr Green, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher maxima sybr green reagent
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Maxima Sybr Green Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher absolute blue sybr green master mix
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Absolute Blue Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 109 article reviews
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    90
    Thermo Fisher 2x power sybr green master mix reagent
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    2x Power Sybr Green Master Mix Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher sybr green fluorescent reagent
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Sybr Green Fluorescent Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dynamo flash sybr green reagent
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Dynamo Flash Sybr Green Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher sybr green fluorophore assay reagents
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Sybr Green Fluorophore Assay Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher alkaline phosphatase alp
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Alkaline Phosphatase Alp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher sybr green er reagent system
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Sybr Green Er Reagent System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher qpcr sybr green reagents
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Qpcr Sybr Green Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher sybr green core reagent kit
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Sybr Green Core Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher 2×sybr green master mix reagent
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    2×Sybr Green Master Mix Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher dynamo sybr green reagents
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Dynamo Sybr Green Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher tris
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Tris, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 11798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher 1x sybr green reagent
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    1x Sybr Green Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher ultrapure distilled water
    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time <t>RT-PCR</t> using <t>SYBR</t> Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
    Ultrapure Distilled Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher sybr green pcr master mix reagents
    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) <t>RT-PCR</t> results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using <t>SYBR</t> Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.
    Sybr Green Pcr Master Mix Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 11822 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher m mgcl2
    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) <t>RT-PCR</t> results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using <t>SYBR</t> Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.
    M Mgcl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reagent kit
    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) <t>RT-PCR</t> results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using <t>SYBR</t> Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.
    Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher sybr green sequence detection reagents
    Expression of S100P and S100PBPR in PanINs, PDACs, CPs, and pancreatic cancer cell lines, as determined by quantitative real-time <t>PCR.</t> Quantitative real-time PCR was conducted on 10-ng cDNA samples using the <t>SYBR</t> Green method and the ABI7700 sequence
    Sybr Green Sequence Detection Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher sybr green qpcr supermix reagents
    Expression of S100P and S100PBPR in PanINs, PDACs, CPs, and pancreatic cancer cell lines, as determined by quantitative real-time <t>PCR.</t> Quantitative real-time PCR was conducted on 10-ng cDNA samples using the <t>SYBR</t> Green method and the ABI7700 sequence
    Sybr Green Qpcr Supermix Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dynamo hs sybr green qpcr kit reagents
    Expression of S100P and S100PBPR in PanINs, PDACs, CPs, and pancreatic cancer cell lines, as determined by quantitative real-time <t>PCR.</t> Quantitative real-time PCR was conducted on 10-ng cDNA samples using the <t>SYBR</t> Green method and the ABI7700 sequence
    Dynamo Hs Sybr Green Qpcr Kit Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher maxima sybr green qpcr master mix
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    Image Search Results


    In vitro differentiation of bone marrow progenitors from miR-15a-deficient mice A. The levels of miR-15a and miR-150 in LSK cells co cultured with OP9 stromal cells at day 11 measured by TaqMan qPCR; B. Representative flow cytometry analysis of DBA, NZB and DBA−/− LSK derived immature (top box) and mature (bottom box) B cell progenitors at day 11 of OP9 co-culture. C. Quantitative flow cytometry analysis of mature AA4.1 − B220 + (right) and immature (left) B cells progenitors. N = 3, columns represent means and bars are SEMs; Transcript expression analysis of D. PU.1, E. Pax5, and F. cMyb measured by qPCR assay are shown. MicroRNA levels were assessed using TaqMan assays with custom probes for has-miR-15a and has-miR-150. Protein-coding genes transcripts measurements were done by qPCR with the use of SYBR green master mix and specific primers; n = 3, columns represent means and bars are SEMs; G. The ratio of PU.1 to Pax5 gene expression in LSK cells at day 11 of co culture with OP9 cells. Asterisks represent statistically significant difference ( p

    Journal: Oncotarget

    Article Title: Role of mir-15a/16-1 in early B cell development in a mouse model of chronic lymphocytic leukemia

    doi: 10.18632/oncotarget.11290

    Figure Lengend Snippet: In vitro differentiation of bone marrow progenitors from miR-15a-deficient mice A. The levels of miR-15a and miR-150 in LSK cells co cultured with OP9 stromal cells at day 11 measured by TaqMan qPCR; B. Representative flow cytometry analysis of DBA, NZB and DBA−/− LSK derived immature (top box) and mature (bottom box) B cell progenitors at day 11 of OP9 co-culture. C. Quantitative flow cytometry analysis of mature AA4.1 − B220 + (right) and immature (left) B cells progenitors. N = 3, columns represent means and bars are SEMs; Transcript expression analysis of D. PU.1, E. Pax5, and F. cMyb measured by qPCR assay are shown. MicroRNA levels were assessed using TaqMan assays with custom probes for has-miR-15a and has-miR-150. Protein-coding genes transcripts measurements were done by qPCR with the use of SYBR green master mix and specific primers; n = 3, columns represent means and bars are SEMs; G. The ratio of PU.1 to Pax5 gene expression in LSK cells at day 11 of co culture with OP9 cells. Asterisks represent statistically significant difference ( p

    Article Snippet: For microRNA measurements, the cDNA was prepared using the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer's protocols.

    Techniques: In Vitro, Mouse Assay, Cell Culture, Real-time Polymerase Chain Reaction, Flow Cytometry, Cytometry, Derivative Assay, Co-Culture Assay, Expressing, SYBR Green Assay

    Repopulation potential of DBA, NZB and DBA −/− congenic mice derived HSC and B1 progenitor cells in immunodeficient NSG recipients at 48 days post injection A. Mir-15a, B. cMyb and C. PU.1 expression levels in DBA, NZB and DBA −/− HSC and B1Ps. MicroRNA levels were assessed using TaqMan assays with custom probes for hsa-miR-15a. Quantitative PCR for protein coding genes was performed with the use of SYBR green master mix and specific primers; n = 3, columns represent means and bars are SEMs. Flow cytometry analysis of D. bone marrow, E. spleen and F. peritoneal cavity cells from NSG recipients. Recipient NSG mice were analyzed 48 days post injection for the presence of donor cells (H2D d+ ) which also expressed markers for B1 progenitors as a percentage of the total donor cell population in the bone marrow and spleen (mean ± SEM, n ≥ 3, p

    Journal: Oncotarget

    Article Title: Role of mir-15a/16-1 in early B cell development in a mouse model of chronic lymphocytic leukemia

    doi: 10.18632/oncotarget.11290

    Figure Lengend Snippet: Repopulation potential of DBA, NZB and DBA −/− congenic mice derived HSC and B1 progenitor cells in immunodeficient NSG recipients at 48 days post injection A. Mir-15a, B. cMyb and C. PU.1 expression levels in DBA, NZB and DBA −/− HSC and B1Ps. MicroRNA levels were assessed using TaqMan assays with custom probes for hsa-miR-15a. Quantitative PCR for protein coding genes was performed with the use of SYBR green master mix and specific primers; n = 3, columns represent means and bars are SEMs. Flow cytometry analysis of D. bone marrow, E. spleen and F. peritoneal cavity cells from NSG recipients. Recipient NSG mice were analyzed 48 days post injection for the presence of donor cells (H2D d+ ) which also expressed markers for B1 progenitors as a percentage of the total donor cell population in the bone marrow and spleen (mean ± SEM, n ≥ 3, p

    Article Snippet: For microRNA measurements, the cDNA was prepared using the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer's protocols.

    Techniques: Mouse Assay, Derivative Assay, Injection, Expressing, Real-time Polymerase Chain Reaction, SYBR Green Assay, Flow Cytometry, Cytometry

    Real-time RT-PCR . Amplification of TNF- α , MIP-2, IL-6 and GAPDH cDNA in livers from mice that were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg) prior to the onset of ischemia (90 min), followed by 3 h of reperfusion (IR). Results are expressed as fluorescence intensity of SYBR Green bound to DNA (Y-axis). The X-axis represents the number of cycles (i.e. 40 cycles). GAPDH was used as a housekeeping gene. NTC represents PCR products amplified in the absence of cDNA template. Graphs shown are representative of three separate experiments with similar results.

    Journal: BMC Clinical Pathology

    Article Title: Protection of early phase hepatic ischemia-reperfusion injury by cholinergic agonists

    doi: 10.1186/1472-6890-6-3

    Figure Lengend Snippet: Real-time RT-PCR . Amplification of TNF- α , MIP-2, IL-6 and GAPDH cDNA in livers from mice that were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg) prior to the onset of ischemia (90 min), followed by 3 h of reperfusion (IR). Results are expressed as fluorescence intensity of SYBR Green bound to DNA (Y-axis). The X-axis represents the number of cycles (i.e. 40 cycles). GAPDH was used as a housekeeping gene. NTC represents PCR products amplified in the absence of cDNA template. Graphs shown are representative of three separate experiments with similar results.

    Article Snippet: Real-Time PCR, using SYBR Green reagent (Applied Biosystems) was performed using Stratagne Mx3000P.

    Techniques: Quantitative RT-PCR, Amplification, Mouse Assay, Fluorescence, SYBR Green Assay, Polymerase Chain Reaction

    Midkine expression was induced by FBS, growth factors and cytokines. A . LNCaP cells were cultured in serum-free DMEM and treated for 48 h with 10% FBS and the indicated agents: 10 ng/ml insulin, 10 ng/ml IGF-I, 10 ng/ml EGF, 10 ng/ml HGF, 10 ng/ml bFGF, 20 ng/ml T3, 10 nM R1881, 10 nM DHT, 10 ng/ml TNFα, 10 ng/ml IL-1β, 50 ng/ml IL-6, 50 ng/ml IL-17, and 33.3 μM RA. B . LNCaP cells were treated with different dosages (1 to 50 ng/ml) of TNFα for 48 h. C . LNCaP cells were also treated with 20 ng/ml TNFα for different time periods (8 to 48 h). 20 μl of each medium supernatant was subjected to Western blot analysis of midkine expression using rabbit anti-midkine antibodies, horseradish peroxidase-conjungated secondary antibodies and enhanced chemiluminescence reagents. D . Total RNA was extracted from LNCaP cells not treated or treated with 20 ng/ml TNFα, using RNeasy Mini Kit; cDNA was made from total RNA using Superscript™ First-Strand Synthesis System with oligo dT primers; real-time quantitative PCR was done in triplicates with Sybr-Green reagents; results were normalized to GAPDH levels as described in Methods; the data (mean ± standard deviation of three independent experiments) were presented as fold change of midkine mRNA compared to the LNCaP cells without treatment for 8 h, where fold = 2 ΔΔCt ; solid bar, TNFα treated; open bar, control; * P

    Journal: BMC Medical Genomics

    Article Title: Midkine is a NF-?B-inducible gene that supports prostate cancer cell survival

    doi: 10.1186/1755-8794-1-6

    Figure Lengend Snippet: Midkine expression was induced by FBS, growth factors and cytokines. A . LNCaP cells were cultured in serum-free DMEM and treated for 48 h with 10% FBS and the indicated agents: 10 ng/ml insulin, 10 ng/ml IGF-I, 10 ng/ml EGF, 10 ng/ml HGF, 10 ng/ml bFGF, 20 ng/ml T3, 10 nM R1881, 10 nM DHT, 10 ng/ml TNFα, 10 ng/ml IL-1β, 50 ng/ml IL-6, 50 ng/ml IL-17, and 33.3 μM RA. B . LNCaP cells were treated with different dosages (1 to 50 ng/ml) of TNFα for 48 h. C . LNCaP cells were also treated with 20 ng/ml TNFα for different time periods (8 to 48 h). 20 μl of each medium supernatant was subjected to Western blot analysis of midkine expression using rabbit anti-midkine antibodies, horseradish peroxidase-conjungated secondary antibodies and enhanced chemiluminescence reagents. D . Total RNA was extracted from LNCaP cells not treated or treated with 20 ng/ml TNFα, using RNeasy Mini Kit; cDNA was made from total RNA using Superscript™ First-Strand Synthesis System with oligo dT primers; real-time quantitative PCR was done in triplicates with Sybr-Green reagents; results were normalized to GAPDH levels as described in Methods; the data (mean ± standard deviation of three independent experiments) were presented as fold change of midkine mRNA compared to the LNCaP cells without treatment for 8 h, where fold = 2 ΔΔCt ; solid bar, TNFα treated; open bar, control; * P

    Article Snippet: Real-time quantitative PCR was done in triplicates with an ABI 7700 Sequence Detector and Sybr-Green reagents (Applied Biosystems) following the recommended protocols [ ].

    Techniques: Expressing, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, SYBR Green Assay, Standard Deviation

    Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time RT-PCR using SYBR Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.

    Journal: Genome Biology

    Article Title: An integrated database of genes responsive to the Myc oncogenic transcription factor: identification of direct genomic targets

    doi:

    Figure Lengend Snippet: Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time RT-PCR using SYBR Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.

    Article Snippet: Real-time PCR For real-time PCR, a SYBR green core reagents kit (Applied Biosystems) or the Failsafe Real-time PCR PreMix selection kit (Epicenter, Madison, WI) were utilized.

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, SYBR Green Assay

    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) RT-PCR results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.

    Journal: Nanoscale Research Letters

    Article Title: Self-assembled HCV core virus-like particles targeted and inhibited tumor cell migration and invasion

    doi: 10.1186/1556-276X-8-401

    Figure Lengend Snippet: Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) RT-PCR results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.

    Article Snippet: Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

    Techniques: Expressing, Recombinant, Reverse Transcription Polymerase Chain Reaction, Isolation, Infection, Synthesized, Quantitative RT-PCR, SYBR Green Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Marker, SDS Page, Western Blot

    Expression of S100P and S100PBPR in PanINs, PDACs, CPs, and pancreatic cancer cell lines, as determined by quantitative real-time PCR. Quantitative real-time PCR was conducted on 10-ng cDNA samples using the SYBR Green method and the ABI7700 sequence

    Journal:

    Article Title: Expression of S100P and Its Novel Binding Partner S100PBPR in Early Pancreatic Cancer

    doi:

    Figure Lengend Snippet: Expression of S100P and S100PBPR in PanINs, PDACs, CPs, and pancreatic cancer cell lines, as determined by quantitative real-time PCR. Quantitative real-time PCR was conducted on 10-ng cDNA samples using the SYBR Green method and the ABI7700 sequence

    Article Snippet: PCR reactions containing 10 ng of cDNA, SYBR Green sequence detection reagents (Applied Biosystems), and gene-specific primers were assayed on an ABI7700 sequence detection system (Applied Biosystems).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, SYBR Green Assay, Sequencing