sybr green pcr master mix Thermo Fisher Search Results


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  • 99
    Thermo Fisher pcr master mix
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    Thermo Fisher platinum multiplex pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher sybr green pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher dreamtaq green pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher taqman fast universal pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher dynazyme ii pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher phusion high fidelity pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher quantitative pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher power sybr green pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher sybr green realtime pcr master mix plus
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Sybr Green Realtime Pcr Master Mix Plus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green taqman universel pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Sybr Green Taqman Universel Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rt2 sybr green pcr master mix reagent
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Rt2 Sybr Green Pcr Master Mix Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman openarray real time pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Taqman Openarray Real Time Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher maxima hot start green pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Maxima Hot Start Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman pcr master mix kit
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Taqman Pcr Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher power pcr sybr green pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Power Pcr Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mouse lrg47 sybr green pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Mouse Lrg47 Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman gene expression pcr master mix
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher sybr green real time pcr master mix plus
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher hotstart it sybr green one step qrt pcr master mix kit
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
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    Thermo Fisher sybr green one step rt pcr master mix reagents
    Bulk validation of <t>SOE-PCR</t> linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.
    Sybr Green One Step Rt Pcr Master Mix Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bulk validation of SOE-PCR linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.

    Journal: Scientific Reports

    Article Title: Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding

    doi: 10.1038/srep44447

    Figure Lengend Snippet: Bulk validation of SOE-PCR linkage of antibody and cell barcode sequences. ( a ) Generation of SOE-PCR product depends on the presence of both antibody tag and cell barcode sequences, as demonstrated on a 1% agarose gel stained with SYBR green. ( b ) The SOE-PCR product is pure, yielding a sharp peak on a Bioanalyzer at the anticipated molecular weight. ( c ) For Abseq to provide quantitative results, the number of SOE-PCR products must be in proportion to the number of antibody tag sequences bound to the cell, which we validate by quantitative PCR. When the appropriate antibody is used, amplification occurs early, indicating presence of much SOE-PCR product (green and red). When no or the incorrect antibody is used, amplification occurs late, indicating little SOE-PCR product.

    Article Snippet: FAM-conjugated probe 3Taq1: 5′ AGA TCG GAA GAG CGT CGT GTA GG 3′ was targeted to the homology domain shared by the antibody and cell barcodes. qPCR was performed using Platinum Multiplex PCR Master Mix (Thermo Fisher Scientific), 2% w/v Tween 20, 2% w/v PEG 6000 and Mx3005P qPCR System (Agilent Technologies).

    Techniques: Overlap Extension Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, SYBR Green Assay, Molecular Weight, Real-time Polymerase Chain Reaction, Amplification

    Microfluidic workflow for single cell protein profiling. ( a ) A two-inlet flow-focus droplet generator encapsulates single cells with proteinase K lysing agent into 47 μm droplets, while a one-inlet droplet maker encapsulates single barcode randomers into 53 μm droplets. (Scale Bar: 400 μm) ( b ) After thermal incubation, these droplets are controllably merged with each other and a PCR droplet using a triple-merger device. Cell and barcode droplets are introduced into two inlets, forming an interdigitated stream prior to spacing by oil (orange). One of each droplet is paired with a large PCR droplet formed upstream (green) and the three droplets electrically merged (blue). The droplets are mixed (pink) and split into two (yellow) and then four (purple) portions, followed by off-chip thermal cycling for SOE-PCR of barcodes to antibody tags.

    Journal: Scientific Reports

    Article Title: Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding

    doi: 10.1038/srep44447

    Figure Lengend Snippet: Microfluidic workflow for single cell protein profiling. ( a ) A two-inlet flow-focus droplet generator encapsulates single cells with proteinase K lysing agent into 47 μm droplets, while a one-inlet droplet maker encapsulates single barcode randomers into 53 μm droplets. (Scale Bar: 400 μm) ( b ) After thermal incubation, these droplets are controllably merged with each other and a PCR droplet using a triple-merger device. Cell and barcode droplets are introduced into two inlets, forming an interdigitated stream prior to spacing by oil (orange). One of each droplet is paired with a large PCR droplet formed upstream (green) and the three droplets electrically merged (blue). The droplets are mixed (pink) and split into two (yellow) and then four (purple) portions, followed by off-chip thermal cycling for SOE-PCR of barcodes to antibody tags.

    Article Snippet: FAM-conjugated probe 3Taq1: 5′ AGA TCG GAA GAG CGT CGT GTA GG 3′ was targeted to the homology domain shared by the antibody and cell barcodes. qPCR was performed using Platinum Multiplex PCR Master Mix (Thermo Fisher Scientific), 2% w/v Tween 20, 2% w/v PEG 6000 and Mx3005P qPCR System (Agilent Technologies).

    Techniques: Flow Cytometry, Incubation, Polymerase Chain Reaction, Chromatin Immunoprecipitation, Overlap Extension Polymerase Chain Reaction