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    Thermo Fisher sybr green pcr master mix
    Impact of nonspecific signal in <t>PCR</t> quantification read-outs. Individual cells expressing or not Gzmb mRNA were amplified simultaneously. ( A ) <t>SYBR</t> Green signal in positive (solid lines) and negative (dotted lines) cells. ( B ) C T evaluation, using the Sequence Detector 1.7 software. The C T value of negative cells and wells not containing template was not significantly different ( t -test: P = 0.18). This program's upper limit of detection is 60 cycles; that is, samples without template score with a C T of 60.
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 102754 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 102754 article reviews
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    sybr green pcr master mix - by Bioz Stars, 2020-07
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    Impact of nonspecific signal in PCR quantification read-outs. Individual cells expressing or not Gzmb mRNA were amplified simultaneously. ( A ) SYBR Green signal in positive (solid lines) and negative (dotted lines) cells. ( B ) C T evaluation, using the Sequence Detector 1.7 software. The C T value of negative cells and wells not containing template was not significantly different ( t -test: P = 0.18). This program's upper limit of detection is 60 cycles; that is, samples without template score with a C T of 60.

    Journal: Genome Research

    Article Title: Quantification of Multiple Gene Expression in Individual Cells

    doi: 10.1101/gr.2890204

    Figure Lengend Snippet: Impact of nonspecific signal in PCR quantification read-outs. Individual cells expressing or not Gzmb mRNA were amplified simultaneously. ( A ) SYBR Green signal in positive (solid lines) and negative (dotted lines) cells. ( B ) C T evaluation, using the Sequence Detector 1.7 software. The C T value of negative cells and wells not containing template was not significantly different ( t -test: P = 0.18). This program's upper limit of detection is 60 cycles; that is, samples without template score with a C T of 60.

    Article Snippet: Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Techniques: Polymerase Chain Reaction, Expressing, Amplification, SYBR Green Assay, Sequencing, Software

    mRNA levels for articular cartilage markers (aggrecan, type II collagen (α1(II)) and catabolic markers (Cox-2, IL-6, iNOS, MMP-13) in vehicle-treated, Wnt3a-treated, IL-1ß-treated, or combined Wnt3a- and IL-1ß-treated human articular chondrocytes overexpressing AnxA6. Human articular chondrocytes were transfected with empty expression vector (EV) or expression vector containing full-length AnxA6 ( AnxA6 ). Twelve hours after transfection cells were serum-starved for 24 h followed by treatment with IL-1ß, Wnt3a, IL-1ß, or combined IL-1ß and Wnt3a for 24h. Levels of mRNA were determined by real-time PCR using SYBR Green and normalized to the level of 18S RNA. The mRNA levels are expressed relative to the level of vehicle-treated cells transfected with empty expression vector, which was set as 1. Data were obtained from triplicate PCRs using RNA from 3 different cultures ( n = 3). Values are the mean ± SD. (**p

    Journal: PLoS ONE

    Article Title: Annexin A6 regulates catabolic events in articular chondrocytes via the modulation of NF-κB and Wnt/ß-catenin signaling

    doi: 10.1371/journal.pone.0197690

    Figure Lengend Snippet: mRNA levels for articular cartilage markers (aggrecan, type II collagen (α1(II)) and catabolic markers (Cox-2, IL-6, iNOS, MMP-13) in vehicle-treated, Wnt3a-treated, IL-1ß-treated, or combined Wnt3a- and IL-1ß-treated human articular chondrocytes overexpressing AnxA6. Human articular chondrocytes were transfected with empty expression vector (EV) or expression vector containing full-length AnxA6 ( AnxA6 ). Twelve hours after transfection cells were serum-starved for 24 h followed by treatment with IL-1ß, Wnt3a, IL-1ß, or combined IL-1ß and Wnt3a for 24h. Levels of mRNA were determined by real-time PCR using SYBR Green and normalized to the level of 18S RNA. The mRNA levels are expressed relative to the level of vehicle-treated cells transfected with empty expression vector, which was set as 1. Data were obtained from triplicate PCRs using RNA from 3 different cultures ( n = 3). Values are the mean ± SD. (**p

    Article Snippet: PCRs were performed with a SYBR Green PCR Master Mix kit (Applied Biosystems), with 95°C for 10 min followed by 40 cycles at 95°C for 15 s and 60°C for 1 min, and 1 cycle at 95°C for 15 s and 60°C for 1 min.

    Techniques: Transfection, Expressing, Plasmid Preparation, Real-time Polymerase Chain Reaction, SYBR Green Assay