sybr green i Thermo Fisher Search Results


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  • 90
    Thermo Fisher sybr green i nucleic acid gel stain
    Analysis of cfDNA concentration in plasma of patients with renal carcinoma and controls. cfDNA concentrations were determined by measuring the fluorescence level of intercalated <t>SYBR</t> <t>Green</t> I dye (a) and by qPCR (c). ROC curve analysis of cfDNA concentration in cancer patients compared with the control group ((b) fluorescence test; (d) qPCR).
    Sybr Green I Nucleic Acid Gel Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher sybr green i
    Visual detection of amplified LAMP products using <t>SYBR</t> green I. Addition of 1 µl of diluted SYBR <t>green</t> I to the reaction tube after LAMP reaction enables visible analysis of the results under natural light ( Figure 1A ) or UV irradiation ( Figure 1B ). The color changes from orange (negative reaction) to green (positive reaction) ( Figure 1A ) and bright fluorescence indicates a positive reaction ( Figure 1B ).
    Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dye sybr green i
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Dye Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 10x sybr green i
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    10x Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green i chemistry
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Sybr Green I Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 1x sybr green i
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    1x Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher fluorophore sybr green i
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Fluorophore Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green i fluorescence
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Sybr Green I Fluorescence, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher continuous sybr green i
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Continuous Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Thermo Fisher sybr green i sbi
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Sybr Green I Sbi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher sybr green i system
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Sybr Green I System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher 000x sybr green i
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    000x Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher sybr green i master
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Sybr Green I Master, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher sybr green i protocol
    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with <t>SYBR</t> <t>Green</t> I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.
    Sybr Green I Protocol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher sybr green i stock
    Detection of SS-LAMP. a Visual Detection of SS-LAMP products using inspection of the color change in 1000 X <t>SYBR</t> <t>Green</t> I by the naked eye under visible and UV lights respectively: Samples that turned yellowish green were considered positive, while those remained orange were assumed to be negative. Tube 1 to 6: SS-LAMP products of Mycobacterium tuberculosis extracted genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). b Lanes 1 to 6: Gel electrophoresis of SS-LAMP products of Mycobacterium tuberculosis genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). M: 1 kb Ladder
    Sybr Green I Stock, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher probe sybr green i
    Detection of SS-LAMP. a Visual Detection of SS-LAMP products using inspection of the color change in 1000 X <t>SYBR</t> <t>Green</t> I by the naked eye under visible and UV lights respectively: Samples that turned yellowish green were considered positive, while those remained orange were assumed to be negative. Tube 1 to 6: SS-LAMP products of Mycobacterium tuberculosis extracted genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). b Lanes 1 to 6: Gel electrophoresis of SS-LAMP products of Mycobacterium tuberculosis genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). M: 1 kb Ladder
    Probe Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher sybr green i core reagent
    Detection of SS-LAMP. a Visual Detection of SS-LAMP products using inspection of the color change in 1000 X <t>SYBR</t> <t>Green</t> I by the naked eye under visible and UV lights respectively: Samples that turned yellowish green were considered positive, while those remained orange were assumed to be negative. Tube 1 to 6: SS-LAMP products of Mycobacterium tuberculosis extracted genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). b Lanes 1 to 6: Gel electrophoresis of SS-LAMP products of Mycobacterium tuberculosis genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). M: 1 kb Ladder
    Sybr Green I Core Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green i solution
    Community analysis of six digesters a – h . Column 1 : Flow cytometric measurement of the unstained samples with the respective autofluorescent subcommunities F420+. Column 2 : The total <t>SYBR</t> <t>Green</t> I stained digester communities. Column 3 : SYBR Green stained subcommunity F420+. Column 4 : the total DAPI stained digester communities. A mcrA targeted T-RFLP analysis of methanogenic archaea in the fresh samples is shown for each digester. Unidentified T-RFs are indicated in grey. The digesters were fed with a disintegrated straw, b whole plant rye silage, c corn silage, d , e chicken manure, f common duckweed, g Elodea nuttallii and h synthetic organic acids. 1,000,000 total events were recorded for unstained samples a – g while 200,000 events were recorded for unstained sample h ; 100,000 cell events were recorded in the SYBR Green I stained samples; 200,000 cell events were recorded in the DAPI stained samples. The black arrow marks the control beads (details in “ Methods ”)
    Sybr Green I Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher 7 5 sybr green i
    Community analysis of six digesters a – h . Column 1 : Flow cytometric measurement of the unstained samples with the respective autofluorescent subcommunities F420+. Column 2 : The total <t>SYBR</t> <t>Green</t> I stained digester communities. Column 3 : SYBR Green stained subcommunity F420+. Column 4 : the total DAPI stained digester communities. A mcrA targeted T-RFLP analysis of methanogenic archaea in the fresh samples is shown for each digester. Unidentified T-RFs are indicated in grey. The digesters were fed with a disintegrated straw, b whole plant rye silage, c corn silage, d , e chicken manure, f common duckweed, g Elodea nuttallii and h synthetic organic acids. 1,000,000 total events were recorded for unstained samples a – g while 200,000 events were recorded for unstained sample h ; 100,000 cell events were recorded in the SYBR Green I stained samples; 200,000 cell events were recorded in the DAPI stained samples. The black arrow marks the control beads (details in “ Methods ”)
    7 5 Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher fluorophore sybr green i kit
    Community analysis of six digesters a – h . Column 1 : Flow cytometric measurement of the unstained samples with the respective autofluorescent subcommunities F420+. Column 2 : The total <t>SYBR</t> <t>Green</t> I stained digester communities. Column 3 : SYBR Green stained subcommunity F420+. Column 4 : the total DAPI stained digester communities. A mcrA targeted T-RFLP analysis of methanogenic archaea in the fresh samples is shown for each digester. Unidentified T-RFs are indicated in grey. The digesters were fed with a disintegrated straw, b whole plant rye silage, c corn silage, d , e chicken manure, f common duckweed, g Elodea nuttallii and h synthetic organic acids. 1,000,000 total events were recorded for unstained samples a – g while 200,000 events were recorded for unstained sample h ; 100,000 cell events were recorded in the SYBR Green I stained samples; 200,000 cell events were recorded in the DAPI stained samples. The black arrow marks the control beads (details in “ Methods ”)
    Fluorophore Sybr Green I Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green i staining
    Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with <t>SYBR</t> Green I.
    Sybr Green I Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pbs sybr green i
    Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with <t>SYBR</t> Green I.
    Pbs Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dyes sybr green i
    Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with <t>SYBR</t> Green I.
    Dyes Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher more sensitive sybr green i
    Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with <t>SYBR</t> Green I.
    More Sensitive Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dsdna dye sybr green i
    Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with <t>SYBR</t> Green I.
    Dsdna Dye Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher sybr green i detection chemistry system
    Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with <t>SYBR</t> Green I.
    Sybr Green I Detection Chemistry System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dna dye sybr green i
    Direct comparison of P. falciparum strains 3D7, Dd2 and FCR3-FMG invasion into RBCs barcoded with DDAO and CellTrace Violet. Equal numbers (1×10 7 ) of RBCs stained with either 5 µM CellTrace DDAO (RBC DDAO ) or CellTrace Violet (RBC Violet ) were combined (total of 2×10 7 RBCs per well) and inoculated with 2×10 5 MACS purified trophozoite stage P. falciparum strains 3D7, Dd2 or FCR3-FMG. Invasion experiments were incubated 18–24 hours to allow for rupture of schizonts and subsequent invasion of merozoites into labeled RBCs, then stained with <t>DNA</t> dye <t>SYBR</t> <t>Green</t> I (to identify pRBCs), fixed and analyzed by flow cytometry. The Susceptibility Index (SI), an unadjusted Odds Ratio assessing the relative risk of RBC DDAO and RBC Violet to 3D7, Dd2, and FCR3-FMG invasion. The marker represents the SI point estimate and the bar represents the 95% confidence interval (CI). A SI of 1.0 indicates no difference in parasite invasion of two RBC populations. Data is the combination of four, seven, and three independent experiments performed in triplicate with 3D7, Dd2, and FCR3-FMG respectively.
    Dna Dye Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher real time sybr green i
    Direct comparison of P. falciparum strains 3D7, Dd2 and FCR3-FMG invasion into RBCs barcoded with DDAO and CellTrace Violet. Equal numbers (1×10 7 ) of RBCs stained with either 5 µM CellTrace DDAO (RBC DDAO ) or CellTrace Violet (RBC Violet ) were combined (total of 2×10 7 RBCs per well) and inoculated with 2×10 5 MACS purified trophozoite stage P. falciparum strains 3D7, Dd2 or FCR3-FMG. Invasion experiments were incubated 18–24 hours to allow for rupture of schizonts and subsequent invasion of merozoites into labeled RBCs, then stained with <t>DNA</t> dye <t>SYBR</t> <t>Green</t> I (to identify pRBCs), fixed and analyzed by flow cytometry. The Susceptibility Index (SI), an unadjusted Odds Ratio assessing the relative risk of RBC DDAO and RBC Violet to 3D7, Dd2, and FCR3-FMG invasion. The marker represents the SI point estimate and the bar represents the 95% confidence interval (CI). A SI of 1.0 indicates no difference in parasite invasion of two RBC populations. Data is the combination of four, seven, and three independent experiments performed in triplicate with 3D7, Dd2, and FCR3-FMG respectively.
    Real Time Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher sybr green i pcr reactions
    Direct comparison of P. falciparum strains 3D7, Dd2 and FCR3-FMG invasion into RBCs barcoded with DDAO and CellTrace Violet. Equal numbers (1×10 7 ) of RBCs stained with either 5 µM CellTrace DDAO (RBC DDAO ) or CellTrace Violet (RBC Violet ) were combined (total of 2×10 7 RBCs per well) and inoculated with 2×10 5 MACS purified trophozoite stage P. falciparum strains 3D7, Dd2 or FCR3-FMG. Invasion experiments were incubated 18–24 hours to allow for rupture of schizonts and subsequent invasion of merozoites into labeled RBCs, then stained with <t>DNA</t> dye <t>SYBR</t> <t>Green</t> I (to identify pRBCs), fixed and analyzed by flow cytometry. The Susceptibility Index (SI), an unadjusted Odds Ratio assessing the relative risk of RBC DDAO and RBC Violet to 3D7, Dd2, and FCR3-FMG invasion. The marker represents the SI point estimate and the bar represents the 95% confidence interval (CI). A SI of 1.0 indicates no difference in parasite invasion of two RBC populations. Data is the combination of four, seven, and three independent experiments performed in triplicate with 3D7, Dd2, and FCR3-FMG respectively.
    Sybr Green I Pcr Reactions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green i reaction mix
    Direct comparison of P. falciparum strains 3D7, Dd2 and FCR3-FMG invasion into RBCs barcoded with DDAO and CellTrace Violet. Equal numbers (1×10 7 ) of RBCs stained with either 5 µM CellTrace DDAO (RBC DDAO ) or CellTrace Violet (RBC Violet ) were combined (total of 2×10 7 RBCs per well) and inoculated with 2×10 5 MACS purified trophozoite stage P. falciparum strains 3D7, Dd2 or FCR3-FMG. Invasion experiments were incubated 18–24 hours to allow for rupture of schizonts and subsequent invasion of merozoites into labeled RBCs, then stained with <t>DNA</t> dye <t>SYBR</t> <t>Green</t> I (to identify pRBCs), fixed and analyzed by flow cytometry. The Susceptibility Index (SI), an unadjusted Odds Ratio assessing the relative risk of RBC DDAO and RBC Violet to 3D7, Dd2, and FCR3-FMG invasion. The marker represents the SI point estimate and the bar represents the 95% confidence interval (CI). A SI of 1.0 indicates no difference in parasite invasion of two RBC populations. Data is the combination of four, seven, and three independent experiments performed in triplicate with 3D7, Dd2, and FCR3-FMG respectively.
    Sybr Green I Reaction Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher moviol sybr green i
    Direct comparison of P. falciparum strains 3D7, Dd2 and FCR3-FMG invasion into RBCs barcoded with DDAO and CellTrace Violet. Equal numbers (1×10 7 ) of RBCs stained with either 5 µM CellTrace DDAO (RBC DDAO ) or CellTrace Violet (RBC Violet ) were combined (total of 2×10 7 RBCs per well) and inoculated with 2×10 5 MACS purified trophozoite stage P. falciparum strains 3D7, Dd2 or FCR3-FMG. Invasion experiments were incubated 18–24 hours to allow for rupture of schizonts and subsequent invasion of merozoites into labeled RBCs, then stained with <t>DNA</t> dye <t>SYBR</t> <t>Green</t> I (to identify pRBCs), fixed and analyzed by flow cytometry. The Susceptibility Index (SI), an unadjusted Odds Ratio assessing the relative risk of RBC DDAO and RBC Violet to 3D7, Dd2, and FCR3-FMG invasion. The marker represents the SI point estimate and the bar represents the 95% confidence interval (CI). A SI of 1.0 indicates no difference in parasite invasion of two RBC populations. Data is the combination of four, seven, and three independent experiments performed in triplicate with 3D7, Dd2, and FCR3-FMG respectively.
    Moviol Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of cfDNA concentration in plasma of patients with renal carcinoma and controls. cfDNA concentrations were determined by measuring the fluorescence level of intercalated SYBR Green I dye (a) and by qPCR (c). ROC curve analysis of cfDNA concentration in cancer patients compared with the control group ((b) fluorescence test; (d) qPCR).

    Journal: Disease Markers

    Article Title: Concentration and Methylation of Cell-Free DNA from Blood Plasma as Diagnostic Markers of Renal Cancer

    doi: 10.1155/2016/3693096

    Figure Lengend Snippet: Analysis of cfDNA concentration in plasma of patients with renal carcinoma and controls. cfDNA concentrations were determined by measuring the fluorescence level of intercalated SYBR Green I dye (a) and by qPCR (c). ROC curve analysis of cfDNA concentration in cancer patients compared with the control group ((b) fluorescence test; (d) qPCR).

    Article Snippet: Specifically, 5 μ L of a sample or the same volume of a standard dilution of genomic DNA (Human HCT116 DKO Nonmethylated DNA) with known concentration (0 ng/mL and 9 serial dilutions from 1 to 256 ng/mL) was added to 195 μ L of a SYBR Green I solution (Cat. number S7585, Thermo Fisher Scientific, USA) in PBS buffer (1 : 10,000) and to black 96-well plates (PAA, Cat. number PAA30296X, Austria) and incubated for 10 min. Two to three identical mixtures were prepared from each sample or standard for greater accuracy.

    Techniques: Concentration Assay, Fluorescence, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Visual detection of amplified LAMP products using SYBR green I. Addition of 1 µl of diluted SYBR green I to the reaction tube after LAMP reaction enables visible analysis of the results under natural light ( Figure 1A ) or UV irradiation ( Figure 1B ). The color changes from orange (negative reaction) to green (positive reaction) ( Figure 1A ) and bright fluorescence indicates a positive reaction ( Figure 1B ).

    Journal: PLoS ONE

    Article Title: Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method without RNA Extraction for the Detection of Human Enterovirus 71 Subgenotype C4 in Nasopharyngeal Swab Specimens

    doi: 10.1371/journal.pone.0052486

    Figure Lengend Snippet: Visual detection of amplified LAMP products using SYBR green I. Addition of 1 µl of diluted SYBR green I to the reaction tube after LAMP reaction enables visible analysis of the results under natural light ( Figure 1A ) or UV irradiation ( Figure 1B ). The color changes from orange (negative reaction) to green (positive reaction) ( Figure 1A ) and bright fluorescence indicates a positive reaction ( Figure 1B ).

    Article Snippet: Similarly, the specificity and sensitivity of both the RT-LAMP and the direct RT-LAMP were performed using the Loopamp RNA amplification kit and the fluorescent detection reagent (FDR) (Eiken Chemical Co., Ltd., Tokyo, Japan) in accordance with the manufacturer's instructions with the exception of the addition of a 1∶100 diluted SYBR Green I (Invitrogen, Eugene Oregon, USA) in place of the FDR after the amplification for the direct RT-LAMP assay.

    Techniques: Amplification, SYBR Green Assay, Irradiation, Fluorescence

    Electrophoresis of the loop-mediated isothermal amplification (LAMP) products and the LAMP products digested with the Hae III restriction enzyme. (A) Gel-imaging analysis of the LAMP product. M: 1 kb plus DNA ladder; Lanes 1–3 indicate positive control, negative control, and LAMP products digested with Hae III, respectively. (B, C) Visual analysis after the addition of SYBR Green I using the naked eye and a UV illuminator, respectively.

    Journal: The Plant Pathology Journal

    Article Title: Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches

    doi: 10.5423/PPJ.OA.07.2019.0197

    Figure Lengend Snippet: Electrophoresis of the loop-mediated isothermal amplification (LAMP) products and the LAMP products digested with the Hae III restriction enzyme. (A) Gel-imaging analysis of the LAMP product. M: 1 kb plus DNA ladder; Lanes 1–3 indicate positive control, negative control, and LAMP products digested with Hae III, respectively. (B, C) Visual analysis after the addition of SYBR Green I using the naked eye and a UV illuminator, respectively.

    Article Snippet: The resulting LAMP amplicons were visualized by adding 1 μl of 5-times diluted original SYBR Green I (Thermo Fisher Scientific, Waltham, MA, USA) to the reaction tube.

    Techniques: Electrophoresis, Amplification, Imaging, Positive Control, Negative Control, SYBR Green Assay

    Schematic illustrations for the device layout and its operation. (A) All the major components of the device, less the circuit. A disposable cartridge, pre-loaded with solid coconut oil at room temperature and a droplet of PCR mixture (within the oil), is connected to the device. (B) The oil melts upon initial heating and the thermocouple loop picks up the droplet (PCR mixture + sample target). The sample solution is added to the PCR mixture droplet using a pipette. (C) In one complete thermal cycle, the droplet moves from the denaturation chamber (98°C), to the annealing chamber (50°C), and then to the extension chamber (80°C). The droplet returns back to the denaturation chamber to commence another cycle. The droplet is guided across the chambers by a thermocouple loop, and it contacts on the Teflon-coated heater surfaces. A PCB heater and a surface-mounted thermocouple control the oil temperature in each chamber. The droplet stays in each chamber until the thermocouple loop detects that it has reached the desired temperature (95°C, 56°C, and 72°C, respectively). (D) A pipette dislodges the droplet upon completion of PCR thermocycling. (E) The thermocouple loop and the metal guide are moved to the extension chamber to secure room for a smartphone microscope. 1 μL of 20× SYBR Green I dye solution is added to the droplet. (F) A smartphone-based fluorescence microscope measures fluorescence. Circuit layout as seen on the breadboards. (A) There are 3 MAX31855, one for each surface-mounted thermocouple. Three JZC-11F relays, one for each heater. The temperature and PID settings are displayed on a 20×4 serial LCD (not shown). (B) The motor controller circuit, showing the AD595 used for the thermocouple loop, which measures internal droplet temperature. Also shown is the EasyDriver connected to the Arduino microcontroller and Haydon-Kerk linear stepper motor. The output of the thermocouple is displayed on another 20×4 serial LCD (not shown). There are 3 buttons, one for starting thermocycling and two for manually positioning the thermocouple loop and droplet. Images created using Fritzing software (Friends of Fritzing e.V., Berlin, Germany). T/C =temperature control.

    Journal: Biosensors & bioelectronics

    Article Title: A Portable, Shock-Proof, Surface-Heated Droplet PCR System for Escherichia coli Detection

    doi: 10.1016/j.bios.2015.06.026

    Figure Lengend Snippet: Schematic illustrations for the device layout and its operation. (A) All the major components of the device, less the circuit. A disposable cartridge, pre-loaded with solid coconut oil at room temperature and a droplet of PCR mixture (within the oil), is connected to the device. (B) The oil melts upon initial heating and the thermocouple loop picks up the droplet (PCR mixture + sample target). The sample solution is added to the PCR mixture droplet using a pipette. (C) In one complete thermal cycle, the droplet moves from the denaturation chamber (98°C), to the annealing chamber (50°C), and then to the extension chamber (80°C). The droplet returns back to the denaturation chamber to commence another cycle. The droplet is guided across the chambers by a thermocouple loop, and it contacts on the Teflon-coated heater surfaces. A PCB heater and a surface-mounted thermocouple control the oil temperature in each chamber. The droplet stays in each chamber until the thermocouple loop detects that it has reached the desired temperature (95°C, 56°C, and 72°C, respectively). (D) A pipette dislodges the droplet upon completion of PCR thermocycling. (E) The thermocouple loop and the metal guide are moved to the extension chamber to secure room for a smartphone microscope. 1 μL of 20× SYBR Green I dye solution is added to the droplet. (F) A smartphone-based fluorescence microscope measures fluorescence. Circuit layout as seen on the breadboards. (A) There are 3 MAX31855, one for each surface-mounted thermocouple. Three JZC-11F relays, one for each heater. The temperature and PID settings are displayed on a 20×4 serial LCD (not shown). (B) The motor controller circuit, showing the AD595 used for the thermocouple loop, which measures internal droplet temperature. Also shown is the EasyDriver connected to the Arduino microcontroller and Haydon-Kerk linear stepper motor. The output of the thermocouple is displayed on another 20×4 serial LCD (not shown). There are 3 buttons, one for starting thermocycling and two for manually positioning the thermocouple loop and droplet. Images created using Fritzing software (Friends of Fritzing e.V., Berlin, Germany). T/C =temperature control.

    Article Snippet: Once the thermocycling is finished, 1 μL of 20× SYBR Green I (SG) dye, prepared from 10,000× SG (Molecular Probes – Life Technologies, Eugene, OR, USA), was added to the reaction droplet using a pipette, resulting in 2× SG in the final solution ( ).

    Techniques: Polymerase Chain Reaction, Transferring, Microscopy, SYBR Green Assay, Fluorescence, Software

    Primase vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and Taq polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.

    Journal: Nucleic Acids Research

    Article Title: A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2

    doi: 10.1093/nar/gkt1385

    Figure Lengend Snippet: Primase vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and Taq polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.

    Article Snippet: Double-stranded DNA synthesized was assayed by adding 1 vol Sybr® Green I (Invitrogen) diluted 1/2500 and measuring fluorescence at 520 nm, with excitation at 480 nm, in a Tecan Infinite 200 microplate reader.

    Techniques: Activity Assay, Incubation, Synthesized, SYBR Green Assay, Fluorescence, Standard Deviation

    Efficiency of intact PolpTN2 and PolpTN2Δ 311–923 in priming DNA synthesis by T. nautilus PolB and Taq DNA polymerase. T. nautilus PolB (8 ng/µl) or Taq polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA (without annealed primer) with increasing concentrations of either intact PolpTN2 ( A ) or PolpTN2 Δ 311–923 ( B ). DNA synthesis by intact PolpTN2 or PolpTN2 Δ 311–923 alone was included as a control. At t = 0 and 6 min, aliquots were withdrawn, quenched in 25 mM EDTA and assayed for ds DNA using Sybr® Green I fluorescence. Circles, continuous line: PolpTN2 or PolpTN2 Δ 311–923 alone; squares, dashed line: PolpTN2 or PolpTN2 Δ 311–923 plus Taq DNA polymerase; triangles, dotted line: PolpTN2 or PolpTN2 Δ 311–923 plus T. nautilus PolB DNA polymerase. Points are the average of three determinations. The standard deviation is indicated by error bars.

    Journal: Nucleic Acids Research

    Article Title: A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2

    doi: 10.1093/nar/gkt1385

    Figure Lengend Snippet: Efficiency of intact PolpTN2 and PolpTN2Δ 311–923 in priming DNA synthesis by T. nautilus PolB and Taq DNA polymerase. T. nautilus PolB (8 ng/µl) or Taq polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA (without annealed primer) with increasing concentrations of either intact PolpTN2 ( A ) or PolpTN2 Δ 311–923 ( B ). DNA synthesis by intact PolpTN2 or PolpTN2 Δ 311–923 alone was included as a control. At t = 0 and 6 min, aliquots were withdrawn, quenched in 25 mM EDTA and assayed for ds DNA using Sybr® Green I fluorescence. Circles, continuous line: PolpTN2 or PolpTN2 Δ 311–923 alone; squares, dashed line: PolpTN2 or PolpTN2 Δ 311–923 plus Taq DNA polymerase; triangles, dotted line: PolpTN2 or PolpTN2 Δ 311–923 plus T. nautilus PolB DNA polymerase. Points are the average of three determinations. The standard deviation is indicated by error bars.

    Article Snippet: Double-stranded DNA synthesized was assayed by adding 1 vol Sybr® Green I (Invitrogen) diluted 1/2500 and measuring fluorescence at 520 nm, with excitation at 480 nm, in a Tecan Infinite 200 microplate reader.

    Techniques: DNA Synthesis, Incubation, SYBR Green Assay, Fluorescence, Standard Deviation

    SYBR Green I/propidium iodide (PI) assay reveals killing of bactericidal and bacteriostatic antibiotics in 30 min. SYBR Green I/PI assay reveals killing between sensitive and resistant strains after treatment with increasing concentrations of bactericidal drugs (A) kanamycin for Staphylococcus aureus and (B) ampicillin for Escherichia coli . Upon treatment with increasing concentrations of bacteriostatic drugs (C) erythromycin for S. aureus and (D) trimethoprim for E. coli , SYBR Green I/PI assay was consistently able to distinguish sensitive and resistant strains when administered at different concentrations for both sensitive and resistant strains (Student’s t -test, * p

    Journal: Frontiers in Medicine

    Article Title: A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay

    doi: 10.3389/fmed.2018.00127

    Figure Lengend Snippet: SYBR Green I/propidium iodide (PI) assay reveals killing of bactericidal and bacteriostatic antibiotics in 30 min. SYBR Green I/PI assay reveals killing between sensitive and resistant strains after treatment with increasing concentrations of bactericidal drugs (A) kanamycin for Staphylococcus aureus and (B) ampicillin for Escherichia coli . Upon treatment with increasing concentrations of bacteriostatic drugs (C) erythromycin for S. aureus and (D) trimethoprim for E. coli , SYBR Green I/PI assay was consistently able to distinguish sensitive and resistant strains when administered at different concentrations for both sensitive and resistant strains (Student’s t -test, * p

    Article Snippet: SYBR Green I/PI Assay SYBR Green I (10,000× stock, Invitrogen) was mixed with PI (20 mM, Sigma) in distilled H2 O.

    Techniques: SYBR Green Assay

    SYBR Green I/propidium iodide monitors the dynamics of resistant and sensitive strains during antibiotic drug exposure. A significant decrease in green/red fluorescence ratio was seen as early as 30 min between (A) sensitive Staphylococcus aureus strain Newman and resistant strain CA127 during kanamycin exposure and (B) sensitive Escherichia coli strain W3110 and resistant strain KTE181 during ampicillin exposure (twoway ANOVA, *** p

    Journal: Frontiers in Medicine

    Article Title: A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay

    doi: 10.3389/fmed.2018.00127

    Figure Lengend Snippet: SYBR Green I/propidium iodide monitors the dynamics of resistant and sensitive strains during antibiotic drug exposure. A significant decrease in green/red fluorescence ratio was seen as early as 30 min between (A) sensitive Staphylococcus aureus strain Newman and resistant strain CA127 during kanamycin exposure and (B) sensitive Escherichia coli strain W3110 and resistant strain KTE181 during ampicillin exposure (twoway ANOVA, *** p

    Article Snippet: SYBR Green I/PI Assay SYBR Green I (10,000× stock, Invitrogen) was mixed with PI (20 mM, Sigma) in distilled H2 O.

    Techniques: SYBR Green Assay, Fluorescence

    SYBR Green I/propidium iodide (PI) stain can distinguish between R (resistant) and S (sensitive) strains of Staphylococcus aureus against various antibiotics in 30 min. Treatment with (A) gentamicin (100 µg/ml), (B) kanamycin (100 µg/ml), (C) erythromycin (400 µg/ml), and (D) ciprofloxacin (100 µg/ml) was added to various overnight S. aureus strains diluted to 1:25 (OD600 = 0.1). After incubation with antibiotics for 30 min, SYBR Green I/PI staining was performed and distinguished the strains and their respective susceptibility categories. All susceptibility results were in concordance with results from the Kirby–Bauer disk diffusion test (Student’s t -test, * p

    Journal: Frontiers in Medicine

    Article Title: A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay

    doi: 10.3389/fmed.2018.00127

    Figure Lengend Snippet: SYBR Green I/propidium iodide (PI) stain can distinguish between R (resistant) and S (sensitive) strains of Staphylococcus aureus against various antibiotics in 30 min. Treatment with (A) gentamicin (100 µg/ml), (B) kanamycin (100 µg/ml), (C) erythromycin (400 µg/ml), and (D) ciprofloxacin (100 µg/ml) was added to various overnight S. aureus strains diluted to 1:25 (OD600 = 0.1). After incubation with antibiotics for 30 min, SYBR Green I/PI staining was performed and distinguished the strains and their respective susceptibility categories. All susceptibility results were in concordance with results from the Kirby–Bauer disk diffusion test (Student’s t -test, * p

    Article Snippet: SYBR Green I/PI Assay SYBR Green I (10,000× stock, Invitrogen) was mixed with PI (20 mM, Sigma) in distilled H2 O.

    Techniques: SYBR Green Assay, Staining, Incubation, Diffusion-based Assay

    SYBR Green I/propidium iodide (PI) stain can distinguish between R (resistant) and S (sensitive) strains of Gram-negative pathogens against various antibiotics in 30 min. Treatment with (A) ampicillin (100 µg/ml), (B) trimethoprim (50 µg/ml), and (C) streptomycin (50 µg/ml) was added to overnight cultures of Escherichia coli strains diluted to 1:25 (OD600 = 0.1). Treatment with (D) ceftriaxone (25 µg/ml) and (E) cefotaxime (50 µg/ml) was added to overnight cultures of Klebsiella pneumoniae strains diluted to 1:25 (OD600 = 0.1). After incubation with antibiotics for 30 min, SYBR Green I/PI staining was performed and distinguished their respective susceptibility categories. All susceptibility results were in concordance with results from the Kirby–Bauer disk diffusion test (Student’s t -test, * p

    Journal: Frontiers in Medicine

    Article Title: A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay

    doi: 10.3389/fmed.2018.00127

    Figure Lengend Snippet: SYBR Green I/propidium iodide (PI) stain can distinguish between R (resistant) and S (sensitive) strains of Gram-negative pathogens against various antibiotics in 30 min. Treatment with (A) ampicillin (100 µg/ml), (B) trimethoprim (50 µg/ml), and (C) streptomycin (50 µg/ml) was added to overnight cultures of Escherichia coli strains diluted to 1:25 (OD600 = 0.1). Treatment with (D) ceftriaxone (25 µg/ml) and (E) cefotaxime (50 µg/ml) was added to overnight cultures of Klebsiella pneumoniae strains diluted to 1:25 (OD600 = 0.1). After incubation with antibiotics for 30 min, SYBR Green I/PI staining was performed and distinguished their respective susceptibility categories. All susceptibility results were in concordance with results from the Kirby–Bauer disk diffusion test (Student’s t -test, * p

    Article Snippet: SYBR Green I/PI Assay SYBR Green I (10,000× stock, Invitrogen) was mixed with PI (20 mM, Sigma) in distilled H2 O.

    Techniques: SYBR Green Assay, Staining, Incubation, Diffusion-based Assay

    Drug susceptibility testing of Mycobacterium tuberculosis against first-line tuberculosis drugs using the SYBR Green I/propidium iodide (PI) assay. (A) M. tuberculosis H37Ra and INH-resistant mutants I2, I4 (10-day old) were treated with 10, 500, and 1,000 µg/ml INH overnight (16 h). INH-resistant mutants I2 and I4 were determined to be resistant using 500 and 1,000 µg/ml INH. (B) M. tuberculosis parental strain H37Ra and pyrazinamide (PZA)-resistant mutants (P5 and P2) (20-day old) were treated with PZA (2 mg/ml) overnight (16 h). Treatment with salicylic acid (40 µg/ml) strongly increased the efficacy SYBR Green I/PI assay in detecting PZA resistance in mutants P5 and P2 compared to parental strain H37Ra (Student’s t -test, * p

    Journal: Frontiers in Medicine

    Article Title: A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay

    doi: 10.3389/fmed.2018.00127

    Figure Lengend Snippet: Drug susceptibility testing of Mycobacterium tuberculosis against first-line tuberculosis drugs using the SYBR Green I/propidium iodide (PI) assay. (A) M. tuberculosis H37Ra and INH-resistant mutants I2, I4 (10-day old) were treated with 10, 500, and 1,000 µg/ml INH overnight (16 h). INH-resistant mutants I2 and I4 were determined to be resistant using 500 and 1,000 µg/ml INH. (B) M. tuberculosis parental strain H37Ra and pyrazinamide (PZA)-resistant mutants (P5 and P2) (20-day old) were treated with PZA (2 mg/ml) overnight (16 h). Treatment with salicylic acid (40 µg/ml) strongly increased the efficacy SYBR Green I/PI assay in detecting PZA resistance in mutants P5 and P2 compared to parental strain H37Ra (Student’s t -test, * p

    Article Snippet: SYBR Green I/PI Assay SYBR Green I (10,000× stock, Invitrogen) was mixed with PI (20 mM, Sigma) in distilled H2 O.

    Techniques: SYBR Green Assay

    A linear relationship between the percentage of live cells and the green/red fluorescence ratio from the SYBR Green I/propidium iodide (PI) viability assay for different bacterial species. Known proportions of isopropyl killed (30 min) and live (A) Staphylococcus aureus (USA300), (B) Klebsiella pneumoniae (Isolate 7), (C) Escherichia coli (W3110), (D) Acinetobacter baumannii , and (E) Mycobacterium tuberculosis (H37Ra) were stained with SYBR Green I/PI and measured using a fluorescence plate reader. A linear regression line was determined. (F) Fluorescence microscopy image showing known proportions (0, 50, and 100%) of live (i–iii) S. aureus (USA300) and (iv–vi) K. pneumoniae (isolate 7) stained with SYBR Green I/PI reveal red (dead) and green (live) ratios in concordance with the known proportions of live and killed organisms from representative Gram-positive ( S. aureus ) and Gram-negative ( K. pneumoniae ) bacteria. Data represent the means ± SEMs.

    Journal: Frontiers in Medicine

    Article Title: A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay

    doi: 10.3389/fmed.2018.00127

    Figure Lengend Snippet: A linear relationship between the percentage of live cells and the green/red fluorescence ratio from the SYBR Green I/propidium iodide (PI) viability assay for different bacterial species. Known proportions of isopropyl killed (30 min) and live (A) Staphylococcus aureus (USA300), (B) Klebsiella pneumoniae (Isolate 7), (C) Escherichia coli (W3110), (D) Acinetobacter baumannii , and (E) Mycobacterium tuberculosis (H37Ra) were stained with SYBR Green I/PI and measured using a fluorescence plate reader. A linear regression line was determined. (F) Fluorescence microscopy image showing known proportions (0, 50, and 100%) of live (i–iii) S. aureus (USA300) and (iv–vi) K. pneumoniae (isolate 7) stained with SYBR Green I/PI reveal red (dead) and green (live) ratios in concordance with the known proportions of live and killed organisms from representative Gram-positive ( S. aureus ) and Gram-negative ( K. pneumoniae ) bacteria. Data represent the means ± SEMs.

    Article Snippet: SYBR Green I/PI Assay SYBR Green I (10,000× stock, Invitrogen) was mixed with PI (20 mM, Sigma) in distilled H2 O.

    Techniques: Fluorescence, SYBR Green Assay, Viability Assay, Staining, Microscopy

    Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with SYBR Green I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.

    Journal: Scientific Reports

    Article Title: Rapid, simple and direct detection of Meloidogyne hapla from infected root galls using loop-mediated isothermal amplification combined with FTA technology

    doi: 10.1038/srep44853

    Figure Lengend Snippet: Loop-mediated isothermal amplification of DNA (LAMP) for detection of M. hapla . ( A ), LAMP products were visually observed with SYBR Green I fluorescence dye. ( B ), 2% agarose Gel electrophoresis separation of LAMP products. ( C ), A lateral flow strips (LFD) detection system was used to detect LAMP amplification. Lines 1–4 represent the M. hapla isolates Mh1, Mh2, Mh3, Mh4, CK, which was the negative control with no template DNA. M represents a DL2000 DNA size marker.

    Article Snippet: Analysis of LAMP products The LAMP amplification results were detected with three methods: adding the fluorescent dye SYBR green I (Invitrogen, 1:1000 TE buffer) to the reaction mixture and visually inspecting the results with the naked eye or under UV light; a Lateral-flow dipstick (LFD) assay that as visually observed with naked eyes, and 2% agarose gel electrophoresis.

    Techniques: Amplification, SYBR Green Assay, Fluorescence, Agarose Gel Electrophoresis, Flow Cytometry, Negative Control, Marker

    The sensitivity of the LAMP assay and the conventional PCR for detection of M. hapla . The two methods were carried out at the following, the negative control used water. The conventional PCR was performed with primers F3 and B3. ( A ) Sensitivity of the LAMP products detected by SYBR Green I fluorescence dye ( B ) Sensitivity of the LAMP products detected by gel electrophoresis. ( C ) Sensitivity of the conventional PCR products detected by gel electrophoresis. Concentrations of 10 −0 , 10 −1 , 10 −2 , 10 −3 , 10 −4 , 10 −5 and 10 −6 of single female nematode genomic DNA were used, CK represents no-template control. Lane M represents a DL2000 DNA size marker (ordinate values in bp).

    Journal: Scientific Reports

    Article Title: Rapid, simple and direct detection of Meloidogyne hapla from infected root galls using loop-mediated isothermal amplification combined with FTA technology

    doi: 10.1038/srep44853

    Figure Lengend Snippet: The sensitivity of the LAMP assay and the conventional PCR for detection of M. hapla . The two methods were carried out at the following, the negative control used water. The conventional PCR was performed with primers F3 and B3. ( A ) Sensitivity of the LAMP products detected by SYBR Green I fluorescence dye ( B ) Sensitivity of the LAMP products detected by gel electrophoresis. ( C ) Sensitivity of the conventional PCR products detected by gel electrophoresis. Concentrations of 10 −0 , 10 −1 , 10 −2 , 10 −3 , 10 −4 , 10 −5 and 10 −6 of single female nematode genomic DNA were used, CK represents no-template control. Lane M represents a DL2000 DNA size marker (ordinate values in bp).

    Article Snippet: Analysis of LAMP products The LAMP amplification results were detected with three methods: adding the fluorescent dye SYBR green I (Invitrogen, 1:1000 TE buffer) to the reaction mixture and visually inspecting the results with the naked eye or under UV light; a Lateral-flow dipstick (LFD) assay that as visually observed with naked eyes, and 2% agarose gel electrophoresis.

    Techniques: Lamp Assay, Polymerase Chain Reaction, Negative Control, SYBR Green Assay, Fluorescence, Nucleic Acid Electrophoresis, Marker

    Application of LAMP and conventional PCR on field samples. The number of samples is identical to that in Supplementary Table 1 . N; negative control. P; positive control. ( A ) LAMP products detected by SYBR Green I fluorescence dye ( B ) LAMP products detected by LFD strip. ( C ) Conventional PCR products detected by gel electrophoresis.

    Journal: Scientific Reports

    Article Title: Rapid, simple and direct detection of Meloidogyne hapla from infected root galls using loop-mediated isothermal amplification combined with FTA technology

    doi: 10.1038/srep44853

    Figure Lengend Snippet: Application of LAMP and conventional PCR on field samples. The number of samples is identical to that in Supplementary Table 1 . N; negative control. P; positive control. ( A ) LAMP products detected by SYBR Green I fluorescence dye ( B ) LAMP products detected by LFD strip. ( C ) Conventional PCR products detected by gel electrophoresis.

    Article Snippet: Analysis of LAMP products The LAMP amplification results were detected with three methods: adding the fluorescent dye SYBR green I (Invitrogen, 1:1000 TE buffer) to the reaction mixture and visually inspecting the results with the naked eye or under UV light; a Lateral-flow dipstick (LFD) assay that as visually observed with naked eyes, and 2% agarose gel electrophoresis.

    Techniques: Polymerase Chain Reaction, Negative Control, Positive Control, SYBR Green Assay, Fluorescence, Stripping Membranes, Nucleic Acid Electrophoresis

    Detection of SS-LAMP. a Visual Detection of SS-LAMP products using inspection of the color change in 1000 X SYBR Green I by the naked eye under visible and UV lights respectively: Samples that turned yellowish green were considered positive, while those remained orange were assumed to be negative. Tube 1 to 6: SS-LAMP products of Mycobacterium tuberculosis extracted genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). b Lanes 1 to 6: Gel electrophoresis of SS-LAMP products of Mycobacterium tuberculosis genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). M: 1 kb Ladder

    Journal: BMC Infectious Diseases

    Article Title: Development and evaluation of an in-house single step loop-mediated isothermal amplification (SS-LAMP) assay for the detection of Mycobacterium tuberculosis complex in sputum samples from Moroccan patients

    doi: 10.1186/s12879-016-1864-9

    Figure Lengend Snippet: Detection of SS-LAMP. a Visual Detection of SS-LAMP products using inspection of the color change in 1000 X SYBR Green I by the naked eye under visible and UV lights respectively: Samples that turned yellowish green were considered positive, while those remained orange were assumed to be negative. Tube 1 to 6: SS-LAMP products of Mycobacterium tuberculosis extracted genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). b Lanes 1 to 6: Gel electrophoresis of SS-LAMP products of Mycobacterium tuberculosis genomic DNA. NC: Negative Control. PC: Positive Control ( M. tuberculosis H37Ra genomic DNA). M: 1 kb Ladder

    Article Snippet: In our present study, the results were visualized by adding 1 μl of 1:10 SYBR Green I stock dilution ($US 0.08/reaction) (Life Technologies, USA) and then, observed under both visible light and UV light.

    Techniques: SYBR Green Assay, Negative Control, Positive Control, Nucleic Acid Electrophoresis

    Community analysis of six digesters a – h . Column 1 : Flow cytometric measurement of the unstained samples with the respective autofluorescent subcommunities F420+. Column 2 : The total SYBR Green I stained digester communities. Column 3 : SYBR Green stained subcommunity F420+. Column 4 : the total DAPI stained digester communities. A mcrA targeted T-RFLP analysis of methanogenic archaea in the fresh samples is shown for each digester. Unidentified T-RFs are indicated in grey. The digesters were fed with a disintegrated straw, b whole plant rye silage, c corn silage, d , e chicken manure, f common duckweed, g Elodea nuttallii and h synthetic organic acids. 1,000,000 total events were recorded for unstained samples a – g while 200,000 events were recorded for unstained sample h ; 100,000 cell events were recorded in the SYBR Green I stained samples; 200,000 cell events were recorded in the DAPI stained samples. The black arrow marks the control beads (details in “ Methods ”)

    Journal: Microbial Cell Factories

    Article Title: Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence

    doi: 10.1186/s12934-017-0793-7

    Figure Lengend Snippet: Community analysis of six digesters a – h . Column 1 : Flow cytometric measurement of the unstained samples with the respective autofluorescent subcommunities F420+. Column 2 : The total SYBR Green I stained digester communities. Column 3 : SYBR Green stained subcommunity F420+. Column 4 : the total DAPI stained digester communities. A mcrA targeted T-RFLP analysis of methanogenic archaea in the fresh samples is shown for each digester. Unidentified T-RFs are indicated in grey. The digesters were fed with a disintegrated straw, b whole plant rye silage, c corn silage, d , e chicken manure, f common duckweed, g Elodea nuttallii and h synthetic organic acids. 1,000,000 total events were recorded for unstained samples a – g while 200,000 events were recorded for unstained sample h ; 100,000 cell events were recorded in the SYBR Green I stained samples; 200,000 cell events were recorded in the DAPI stained samples. The black arrow marks the control beads (details in “ Methods ”)

    Article Snippet: The staining was performed in 800-µL batches containing 5 µL sample solution, 735 µL PBS, 40 µL ethanol, and 20 µL 20× SYBR-Green I solution (ThermoFisher Scientific, Waltham, Massachusetts, USA).

    Techniques: Flow Cytometry, SYBR Green Assay, Staining

    Flow cytometric analysis of a methanogenic enrichment culture (MEC) with sort gates in black ( a unstained, b – e stained). a FSC vs. F420+. Subcommunities with high autofluorescent (MEC F420+) and low autofluorescent properties (MEC F420low) can be detected. b 3D visualization of FSC vs. F420+ vs. SYBR Green I. c FSC vs. SYBR Green I plot of the total microbial community. d F420+ vs. SYBR Green I plot used for discrimination of high (MEC F420 + S1 and MEC F420 + S2) as well as low and non-autofluorescent subcommunities (MEC F420− and MEC MF420low. e Position of subcommunities MEC F420 + S1 and MEC F420 + S2 in a FSC vs. SYBR Green I plot. The arrow marks the control beads

    Journal: Microbial Cell Factories

    Article Title: Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence

    doi: 10.1186/s12934-017-0793-7

    Figure Lengend Snippet: Flow cytometric analysis of a methanogenic enrichment culture (MEC) with sort gates in black ( a unstained, b – e stained). a FSC vs. F420+. Subcommunities with high autofluorescent (MEC F420+) and low autofluorescent properties (MEC F420low) can be detected. b 3D visualization of FSC vs. F420+ vs. SYBR Green I. c FSC vs. SYBR Green I plot of the total microbial community. d F420+ vs. SYBR Green I plot used for discrimination of high (MEC F420 + S1 and MEC F420 + S2) as well as low and non-autofluorescent subcommunities (MEC F420− and MEC MF420low. e Position of subcommunities MEC F420 + S1 and MEC F420 + S2 in a FSC vs. SYBR Green I plot. The arrow marks the control beads

    Article Snippet: The staining was performed in 800-µL batches containing 5 µL sample solution, 735 µL PBS, 40 µL ethanol, and 20 µL 20× SYBR-Green I solution (ThermoFisher Scientific, Waltham, Massachusetts, USA).

    Techniques: Flow Cytometry, Staining, SYBR Green Assay

    Influence of nucleic acid staining on F 420 fluorescence. a Unstained digester sample after 3 h as a control. b The same sample after 3 h of SYBR Green I staining. c Cell numbers of the subcommunities F420+ (white bar), F420− (light grey bar) and autofluorescence intensity (dark grey bar) of the subcommunities F420+ are indicated with the respective standard deviations. Samples were gated according to Additional file 1 : Figure S4. Values are given in Additional file 1 : S7. The arrow marks the added control beads

    Journal: Microbial Cell Factories

    Article Title: Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence

    doi: 10.1186/s12934-017-0793-7

    Figure Lengend Snippet: Influence of nucleic acid staining on F 420 fluorescence. a Unstained digester sample after 3 h as a control. b The same sample after 3 h of SYBR Green I staining. c Cell numbers of the subcommunities F420+ (white bar), F420− (light grey bar) and autofluorescence intensity (dark grey bar) of the subcommunities F420+ are indicated with the respective standard deviations. Samples were gated according to Additional file 1 : Figure S4. Values are given in Additional file 1 : S7. The arrow marks the added control beads

    Article Snippet: The staining was performed in 800-µL batches containing 5 µL sample solution, 735 µL PBS, 40 µL ethanol, and 20 µL 20× SYBR-Green I solution (ThermoFisher Scientific, Waltham, Massachusetts, USA).

    Techniques: Staining, Fluorescence, SYBR Green Assay

    Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with SYBR Green I.

    Journal: Nucleic Acids Research

    Article Title: Exponential propagation of large circular DNA by reconstitution of a chromosome-replication cycle

    doi: 10.1093/nar/gkx822

    Figure Lengend Snippet: Monoclonal propagation of DNA by limiting-dilution. Mixtures of pOri8 (9.5 kb) and pOriDif (12 kb) after limiting dilution (15 molecules of each in samples 1–3, an average of 1.5 molecules of each in samples 4–7) in the RCR mixture (10 μl) were incubated for 6 h. pOri8 and pOriDif were also individually propagated. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with SYBR Green I.

    Article Snippet: An aliquot (2 μl) was analyzed by 0.5% agarose-gel electrophoresis followed by SYBR Green I staining (Molecular Probes) or by phosphor imaging.

    Techniques: Incubation, Marker, Derivative Assay, Staining, SYBR Green Assay

    Continuous repetition of the RCR. ( A ) The indicated amount of pOri8 (9.5 kb) was incubated in the RCR mixture for 3 h. Aliquots (1 μl) were detected by agarose-gel electrophoresis and SYBR Green staining. The input DNA (10 9 molecules in 10 μl, 1 ng/μl) was also detected (‘no RCR’). Size-marker fragments (M1) were derived from phage λ DNA. ( B ) pOri8 (10 5 molecules/μl) was incubated in the RCR mixture at 30°C. Aliquots (1 μl) were taken at the indicated times and the number of DNA circles was quantified using the transformation method. The RCR mixture was preincubated at 30°C for 15 min before the addition of template DNA. The values from three independent reactions are shown with the error bars (standard error of the mean). ( C ) pPKOZ (10 −5 ng, 8.9 kb) was incubated in the RCR mixture for 3 h (passage 1). The sample was then diluted 10 6 -fold in the fresh RCR mixture and further incubated for 3 h (passage 2). This sequence was repeated for a total of 10 incubations. At each passage, aliquots were analyzed. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with SYBR Green I. ( D ) The total doublings were deduced using the transformation method. Error rates per base per replication cycle were deduced by blue–white determination of the lacZ status ( 28 ). Mutagenic dNTPs, when included, consisted of 1 μM each of 8-oxo-GTP and dPTP. The transformation analysis and the blue–white determination were performed twice and the mean values were shown with the ranges.

    Journal: Nucleic Acids Research

    Article Title: Exponential propagation of large circular DNA by reconstitution of a chromosome-replication cycle

    doi: 10.1093/nar/gkx822

    Figure Lengend Snippet: Continuous repetition of the RCR. ( A ) The indicated amount of pOri8 (9.5 kb) was incubated in the RCR mixture for 3 h. Aliquots (1 μl) were detected by agarose-gel electrophoresis and SYBR Green staining. The input DNA (10 9 molecules in 10 μl, 1 ng/μl) was also detected (‘no RCR’). Size-marker fragments (M1) were derived from phage λ DNA. ( B ) pOri8 (10 5 molecules/μl) was incubated in the RCR mixture at 30°C. Aliquots (1 μl) were taken at the indicated times and the number of DNA circles was quantified using the transformation method. The RCR mixture was preincubated at 30°C for 15 min before the addition of template DNA. The values from three independent reactions are shown with the error bars (standard error of the mean). ( C ) pPKOZ (10 −5 ng, 8.9 kb) was incubated in the RCR mixture for 3 h (passage 1). The sample was then diluted 10 6 -fold in the fresh RCR mixture and further incubated for 3 h (passage 2). This sequence was repeated for a total of 10 incubations. At each passage, aliquots were analyzed. Size-marker fragments (M1) were derived from phage λ DNA. DNA was visualized by staining with SYBR Green I. ( D ) The total doublings were deduced using the transformation method. Error rates per base per replication cycle were deduced by blue–white determination of the lacZ status ( 28 ). Mutagenic dNTPs, when included, consisted of 1 μM each of 8-oxo-GTP and dPTP. The transformation analysis and the blue–white determination were performed twice and the mean values were shown with the ranges.

    Article Snippet: An aliquot (2 μl) was analyzed by 0.5% agarose-gel electrophoresis followed by SYBR Green I staining (Molecular Probes) or by phosphor imaging.

    Techniques: Incubation, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Marker, Derivative Assay, Transformation Assay, Sequencing

    Direct comparison of P. falciparum strains 3D7, Dd2 and FCR3-FMG invasion into RBCs barcoded with DDAO and CellTrace Violet. Equal numbers (1×10 7 ) of RBCs stained with either 5 µM CellTrace DDAO (RBC DDAO ) or CellTrace Violet (RBC Violet ) were combined (total of 2×10 7 RBCs per well) and inoculated with 2×10 5 MACS purified trophozoite stage P. falciparum strains 3D7, Dd2 or FCR3-FMG. Invasion experiments were incubated 18–24 hours to allow for rupture of schizonts and subsequent invasion of merozoites into labeled RBCs, then stained with DNA dye SYBR Green I (to identify pRBCs), fixed and analyzed by flow cytometry. The Susceptibility Index (SI), an unadjusted Odds Ratio assessing the relative risk of RBC DDAO and RBC Violet to 3D7, Dd2, and FCR3-FMG invasion. The marker represents the SI point estimate and the bar represents the 95% confidence interval (CI). A SI of 1.0 indicates no difference in parasite invasion of two RBC populations. Data is the combination of four, seven, and three independent experiments performed in triplicate with 3D7, Dd2, and FCR3-FMG respectively.

    Journal: PLoS ONE

    Article Title: RBC Barcoding Allows for the Study of Erythrocyte Population Dynamics and P. falciparum Merozoite Invasion

    doi: 10.1371/journal.pone.0101041

    Figure Lengend Snippet: Direct comparison of P. falciparum strains 3D7, Dd2 and FCR3-FMG invasion into RBCs barcoded with DDAO and CellTrace Violet. Equal numbers (1×10 7 ) of RBCs stained with either 5 µM CellTrace DDAO (RBC DDAO ) or CellTrace Violet (RBC Violet ) were combined (total of 2×10 7 RBCs per well) and inoculated with 2×10 5 MACS purified trophozoite stage P. falciparum strains 3D7, Dd2 or FCR3-FMG. Invasion experiments were incubated 18–24 hours to allow for rupture of schizonts and subsequent invasion of merozoites into labeled RBCs, then stained with DNA dye SYBR Green I (to identify pRBCs), fixed and analyzed by flow cytometry. The Susceptibility Index (SI), an unadjusted Odds Ratio assessing the relative risk of RBC DDAO and RBC Violet to 3D7, Dd2, and FCR3-FMG invasion. The marker represents the SI point estimate and the bar represents the 95% confidence interval (CI). A SI of 1.0 indicates no difference in parasite invasion of two RBC populations. Data is the combination of four, seven, and three independent experiments performed in triplicate with 3D7, Dd2, and FCR3-FMG respectively.

    Article Snippet: Following merozoite invasion, cells were stained with 1× DNA dye SYBR Green I (Invitrogen), fixed with 1% paraformaldehyde and 0.0075% glutaraldehyde in Alsever's Solution (Sigma) as described previously , and analyzed by flow cytometry or microscopy.

    Techniques: Staining, Magnetic Cell Separation, Purification, Incubation, Labeling, SYBR Green Assay, Flow Cytometry, Cytometry, Marker

    RBCs barcoded with CellTrace DDAO and Violet can be combined to directly compare P. falciparum invasion in an invasion assay. RBCs were labeled with 5 µM of either DDAO (A) or CellTrace Violet (B). Cells were then combined and infected with MACS purified unlabeled pRBCs. Experiments were incubated 18–24 hours. Cells were then stained with DNA dye SYBR Green I, fixed, and examined by brightfield (C) and fluorescence microscopy (D, E) and by flow cytometry (F–H). (A) shows red channel only, (B) shows violet channel only, (C) shows brightfield, (D) shows green channel only and (E) shows merge of red, violet, and green channels. (F) Flow cytometry plot of RBCs stained with CellTrace DDAO (R1) and CellTrace Violet (R4) and non-stained pRBC (R3). (G) Flow cytometry plot shows DDAO negative pRBCs (R5), DDAO negative uninfected RBCs (R7), DDAO positive pRBCs (R6) and DDAO positive uninfected RBCs (R8). (H) Flow cytometry plot shows CellTrace Violet negative pRBCs (R9), CellTrace Violet negative uninfected RBCs (R11), CellTrace Violet positive pRBCs (R10) and CellTrace Violet positive uninfected RBCs (R12).

    Journal: PLoS ONE

    Article Title: RBC Barcoding Allows for the Study of Erythrocyte Population Dynamics and P. falciparum Merozoite Invasion

    doi: 10.1371/journal.pone.0101041

    Figure Lengend Snippet: RBCs barcoded with CellTrace DDAO and Violet can be combined to directly compare P. falciparum invasion in an invasion assay. RBCs were labeled with 5 µM of either DDAO (A) or CellTrace Violet (B). Cells were then combined and infected with MACS purified unlabeled pRBCs. Experiments were incubated 18–24 hours. Cells were then stained with DNA dye SYBR Green I, fixed, and examined by brightfield (C) and fluorescence microscopy (D, E) and by flow cytometry (F–H). (A) shows red channel only, (B) shows violet channel only, (C) shows brightfield, (D) shows green channel only and (E) shows merge of red, violet, and green channels. (F) Flow cytometry plot of RBCs stained with CellTrace DDAO (R1) and CellTrace Violet (R4) and non-stained pRBC (R3). (G) Flow cytometry plot shows DDAO negative pRBCs (R5), DDAO negative uninfected RBCs (R7), DDAO positive pRBCs (R6) and DDAO positive uninfected RBCs (R8). (H) Flow cytometry plot shows CellTrace Violet negative pRBCs (R9), CellTrace Violet negative uninfected RBCs (R11), CellTrace Violet positive pRBCs (R10) and CellTrace Violet positive uninfected RBCs (R12).

    Article Snippet: Following merozoite invasion, cells were stained with 1× DNA dye SYBR Green I (Invitrogen), fixed with 1% paraformaldehyde and 0.0075% glutaraldehyde in Alsever's Solution (Sigma) as described previously , and analyzed by flow cytometry or microscopy.

    Techniques: Invasion Assay, Labeling, Infection, Magnetic Cell Separation, Purification, Incubation, Staining, SYBR Green Assay, Fluorescence, Microscopy, Flow Cytometry, Cytometry