sybr green i Millipore Search Results


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  • 95
    Millipore sybr green i jumpstart taq readymix
    Sybr Green I Jumpstart Taq Readymix, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 1x sybr green i
    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via <t>SYBR</t> <t>Green</t> I real-time PCR. a , amplification curves; b , melting curve analysis
    1x Sybr Green I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 0 6x sybr green i
    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via <t>SYBR</t> <t>Green</t> I real-time PCR. a , amplification curves; b , melting curve analysis
    0 6x Sybr Green I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Millipore sybr green i staining
    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via <t>SYBR</t> <t>Green</t> I real-time PCR. a , amplification curves; b , melting curve analysis
    Sybr Green I Staining, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 0 25x sybr green i
    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via <t>SYBR</t> <t>Green</t> I real-time PCR. a , amplification curves; b , melting curve analysis
    0 25x Sybr Green I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sybr green i dna
    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via <t>SYBR</t> <t>Green</t> I real-time PCR. a , amplification curves; b , melting curve analysis
    Sybr Green I Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sybr green i fluorescence kit
    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via <t>SYBR</t> <t>Green</t> I real-time PCR. a , amplification curves; b , melting curve analysis
    Sybr Green I Fluorescence Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sybr green i fluorescence
    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via <t>SYBR</t> <t>Green</t> I real-time PCR. a , amplification curves; b , melting curve analysis
    Sybr Green I Fluorescence, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore cell permeant sybr green i 1x
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    Cell Permeant Sybr Green I 1x, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore x stock sybr green i
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    X Stock Sybr Green I, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore lightcycler 480 sybr green i master
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    Lightcycler 480 Sybr Green I Master, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore strand specific dna binding dye sybr green i
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    Strand Specific Dna Binding Dye Sybr Green I, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore lysis buffer
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    Lysis Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 79760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore chemistry
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    Chemistry, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore jumpstart taq readymix kit
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    Jumpstart Taq Readymix Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore faststart sybr green master
    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of <t>SYBR</t> <t>Green</t> I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.
    Faststart Sybr Green Master, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via SYBR Green I real-time PCR. a , amplification curves; b , melting curve analysis

    Journal: BMC Genomics

    Article Title: Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense

    doi: 10.1186/s12864-015-1978-2

    Figure Lengend Snippet: Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via SYBR Green I real-time PCR. a , amplification curves; b , melting curve analysis

    Article Snippet: A 10 μl reaction mixture was prepared for each sample, with the following components: 1 μl of Taq buffer (including 20 mM MgCl2 ) supplied together with Ex Taq HS (Takara), 0.25 μM forward primer, 0.25 μM reverse primer, 0.2 mM dNTPs, 0.7 mg/ml BSA (NEB), 0.5 × SYBR Green I (Sigma S9430), 3 % DMSO, 0.25 U of ExTaq HS, and sterile distilled water.

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Amplification

    Real-time monitoring of preamplification. ( a ) Response curve for real-time monitoring of adapter-based preamplification using SYBR Green I detection chemistry following full-length reverse transcription (RT). ( b ) Response curve for real-time monitoring of multiplex preamplification using SYBR Green I detection chemistry following universal RT. cDNA corresponding to 30 pg total RNA isolated from MLS 2645–94 cells was used in each preamplification reaction. Melting curves for adaptor-based and multiplex preamplification are shown in ( c ) and ( d ), respectively. RT no-template controls (NTCs) and preamplification NTCs were included as references to distinguish specific from non-specific PCR products and to determine in what reactions these products were generated. –d(Rel. fluoresc.)/d(Temp.), –d(Relative fluorescence)/d(Temperature).

    Journal: Scientific Reports

    Article Title: Global preamplification simplifies targeted mRNA quantification

    doi: 10.1038/srep45219

    Figure Lengend Snippet: Real-time monitoring of preamplification. ( a ) Response curve for real-time monitoring of adapter-based preamplification using SYBR Green I detection chemistry following full-length reverse transcription (RT). ( b ) Response curve for real-time monitoring of multiplex preamplification using SYBR Green I detection chemistry following universal RT. cDNA corresponding to 30 pg total RNA isolated from MLS 2645–94 cells was used in each preamplification reaction. Melting curves for adaptor-based and multiplex preamplification are shown in ( c ) and ( d ), respectively. RT no-template controls (NTCs) and preamplification NTCs were included as references to distinguish specific from non-specific PCR products and to determine in what reactions these products were generated. –d(Rel. fluoresc.)/d(Temp.), –d(Relative fluorescence)/d(Temperature).

    Article Snippet: Both preamplification approaches were performed as described above, except for the global preamplification which was adapted to real-time monitoring by adding 0.1x (final concentration) SYBR Green I (Sigma-Aldrich) to the reaction.

    Techniques: SYBR Green Assay, Multiplex Assay, Isolation, Polymerase Chain Reaction, Generated, Fluorescence

    Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of SYBR Green I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.

    Journal: Frontiers in Microbiology

    Article Title: Role of Temperate Bacteriophage ϕ20617 on Streptococcus thermophilus DSM 20617T Autolysis and Biology

    doi: 10.3389/fmicb.2018.02719

    Figure Lengend Snippet: Growth of Streptococcus thermophilus DSM 20617 T (squares) and its derivative A33 (circles) strain in M17 broth in the presence of sucrose, and lactose. (A) Growth of S. thermophilus strains in the presence of sucrose at a final concentration of 0.2% (white symbols) and 1% (black symbols). Strain DSM 20617 T (autolytic) (white and black squares), strain A33 (non-autolytic) (white and black circles). Flow cytometry of SYBR Green I stained cells of S. thermophilus DSM 20617 T grown in M17 (0.2 % wt/v sucrose). (B) Cells after 4 h of growth at 37°C (blue gate). (C) Cells and cell debris (red gate) after 8 h of growth at 37°C. (D) Cells and cell debris (red gate) after 8 h of growth at 37°C treated with DNAse. (E) M17 broth SYBR Green I stained.

    Article Snippet: Flow Cytometry Measurements The cell suspensions were diluted in sterile filtered (0.2 μm) water and stained with a cell-permeant SYBR green I (1X) (Sigma-Aldrich, Milan, Italy) or to a double staining using SYBR green I and propidium iodide (PI) ( ).

    Techniques: Concentration Assay, Flow Cytometry, Cytometry, SYBR Green Assay, Staining