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  • 92
    Amresco sybr green i
    Sybr Green I, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beijing CWBio sybr green i
    Sybr Green I, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad sybr green i
    Sybr Green I, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 763 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cambrex sybr green i
    Visual appearance of LAMP reactions from isolates after addition of <t>SYBR</t> Green I. (a) Positive reaction (tube 1), negative reaction (tube 2) and tube without DNA templates (tube 3). (b) Under UV transillumination, positive reaction (tube 4), negative reaction (tube 5) and tube without DNA templates (tube 6).
    Sybr Green I, supplied by Cambrex, used in various techniques. Bioz Stars score: 92/100, based on 394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kaneka Corp sybr green i
    Visual appearance of LAMP reactions from isolates after addition of <t>SYBR</t> Green I. (a) Positive reaction (tube 1), negative reaction (tube 2) and tube without DNA templates (tube 3). (b) Under UV transillumination, positive reaction (tube 4), negative reaction (tube 5) and tube without DNA templates (tube 6).
    Sybr Green I, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 91/100, based on 1160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Ribobio sybr green i
    Visual appearance of LAMP reactions from isolates after addition of <t>SYBR</t> Green I. (a) Positive reaction (tube 1), negative reaction (tube 2) and tube without DNA templates (tube 3). (b) Under UV transillumination, positive reaction (tube 4), negative reaction (tube 5) and tube without DNA templates (tube 6).
    Sybr Green I, supplied by Ribobio, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Roche sybr green i
    Effect of Cymbidium ethanol extract (CYM) on 2,4-dinitrochlorobenzene- (DNCB-) induced expression of interleukin- (IL-) 4, IL-13, and tumor necrosis factor- (TNF-) α mRNA in atopic dermatitis- (AD-) like mouse model. After inducing AD, 10 mg/mL CYM solution (in 3 : 1 mixture of acetone and olive oil) was applied to the dorsal skin of mice for a total of 6 times over a 2-week period. After euthanasia, dorsal skin lesions were enucleated from mice of all three groups (non-, DNCB, DNCB, and CYM treatment). Total RNA was isolated from extracted tissue of dorsal lesions using Tri-reagent. Total RNA was used as a template for cDNA synthesis, which was performed using a cDNA Synthesis kit. Real-time polymerase chain reaction (qPCR) analysis was carried out using <t>SYBR</t> <t>Green</t> I and a Lightcycler 96 instrument. The qPCR analysis was performed to detect IL-4, IL-13, and TNF- α mRNA expression. Data are mean ± standard deviation (SD, n = 6). ∗ P
    Sybr Green I, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 1948 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene sybr green i
    Effect of Cymbidium ethanol extract (CYM) on 2,4-dinitrochlorobenzene- (DNCB-) induced expression of interleukin- (IL-) 4, IL-13, and tumor necrosis factor- (TNF-) α mRNA in atopic dermatitis- (AD-) like mouse model. After inducing AD, 10 mg/mL CYM solution (in 3 : 1 mixture of acetone and olive oil) was applied to the dorsal skin of mice for a total of 6 times over a 2-week period. After euthanasia, dorsal skin lesions were enucleated from mice of all three groups (non-, DNCB, DNCB, and CYM treatment). Total RNA was isolated from extracted tissue of dorsal lesions using Tri-reagent. Total RNA was used as a template for cDNA synthesis, which was performed using a cDNA Synthesis kit. Real-time polymerase chain reaction (qPCR) analysis was carried out using <t>SYBR</t> <t>Green</t> I and a Lightcycler 96 instrument. The qPCR analysis was performed to detect IL-4, IL-13, and TNF- α mRNA expression. Data are mean ± standard deviation (SD, n = 6). ∗ P
    Sybr Green I, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa sybr green i
    Microbial distribution in a saponite-bearing locus near basaltic groundmass revealed by staining of a thin section with <t>SYBR-Green</t> I. (A) Back-scattered electron image of saponite aggregates enlarged from Figure 2B . An orange rectangle indicates the area shown in (B) . Fluorescence microscopy images of SYBR Green I-stained microbial cells associated with saponite in a 100-μm thin section of a rock piece (B) and in a 3-μm thin section of a clay fraction (C) .
    Sybr Green I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1608 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 11630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Toyobo sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Toyobo, used in various techniques. Bioz Stars score: 93/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Trevigen sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Trevigen, used in various techniques. Bioz Stars score: 92/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioteke Corporation sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Evrogen sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Evrogen, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Genecopoeia sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech sybr green i
    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all <t>SYBR</t> <t>Green</t> I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.
    Sybr Green I, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Generay Biotech sybr green i
    Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with <t>SYBR</t> Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.
    Sybr Green I, supplied by Shanghai Generay Biotech, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tektronix inc sybr green i
    Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with <t>SYBR</t> Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.
    Sybr Green I, supplied by Tektronix inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co sybr green i
    Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with <t>SYBR</t> Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.
    Sybr Green I, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioGenes GmbH sybr green i
    Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with <t>SYBR</t> Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.
    Sybr Green I, supplied by BioGenes GmbH, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotium sybr green i
    Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with <t>SYBR</t> Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.
    Sybr Green I, supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biozym sybr green i
    Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with <t>SYBR</t> Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.
    Sybr Green I, supplied by Biozym, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Visual appearance of LAMP reactions from isolates after addition of SYBR Green I. (a) Positive reaction (tube 1), negative reaction (tube 2) and tube without DNA templates (tube 3). (b) Under UV transillumination, positive reaction (tube 4), negative reaction (tube 5) and tube without DNA templates (tube 6).

    Journal: Fems Immunology and Medical Microbiology

    Article Title: Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples

    doi: 10.1111/j.1574-695X.2009.00647.x

    Figure Lengend Snippet: Visual appearance of LAMP reactions from isolates after addition of SYBR Green I. (a) Positive reaction (tube 1), negative reaction (tube 2) and tube without DNA templates (tube 3). (b) Under UV transillumination, positive reaction (tube 4), negative reaction (tube 5) and tube without DNA templates (tube 6).

    Article Snippet: LAMP reaction products were made visible by the addition of 2.0 µL of 10-fold diluted SYBR Green I (Cambrex Bio Science, Wokingham, UK) to each reaction tube separately; the change in the color of the solution was observed directly by the naked eye or using a UV transilluminator.

    Techniques: SYBR Green Assay

    Visual sensitivity of LAMP reactions using SYBR Green I. (a) Direct detection by the naked eye, (b) under UV transillumination. Tubes 1–7, 2 × 10 5 , 2 × 10 4 , 2 × 10 3 , 2 × 10 2 , 2 × 10 1 , 2 × 10 0 , 2 × 10 −1 copies per tube, respectively; tube 8, negative control without DNA.

    Journal: Fems Immunology and Medical Microbiology

    Article Title: Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples

    doi: 10.1111/j.1574-695X.2009.00647.x

    Figure Lengend Snippet: Visual sensitivity of LAMP reactions using SYBR Green I. (a) Direct detection by the naked eye, (b) under UV transillumination. Tubes 1–7, 2 × 10 5 , 2 × 10 4 , 2 × 10 3 , 2 × 10 2 , 2 × 10 1 , 2 × 10 0 , 2 × 10 −1 copies per tube, respectively; tube 8, negative control without DNA.

    Article Snippet: LAMP reaction products were made visible by the addition of 2.0 µL of 10-fold diluted SYBR Green I (Cambrex Bio Science, Wokingham, UK) to each reaction tube separately; the change in the color of the solution was observed directly by the naked eye or using a UV transilluminator.

    Techniques: SYBR Green Assay, Negative Control

    Effect of Cymbidium ethanol extract (CYM) on 2,4-dinitrochlorobenzene- (DNCB-) induced expression of interleukin- (IL-) 4, IL-13, and tumor necrosis factor- (TNF-) α mRNA in atopic dermatitis- (AD-) like mouse model. After inducing AD, 10 mg/mL CYM solution (in 3 : 1 mixture of acetone and olive oil) was applied to the dorsal skin of mice for a total of 6 times over a 2-week period. After euthanasia, dorsal skin lesions were enucleated from mice of all three groups (non-, DNCB, DNCB, and CYM treatment). Total RNA was isolated from extracted tissue of dorsal lesions using Tri-reagent. Total RNA was used as a template for cDNA synthesis, which was performed using a cDNA Synthesis kit. Real-time polymerase chain reaction (qPCR) analysis was carried out using SYBR Green I and a Lightcycler 96 instrument. The qPCR analysis was performed to detect IL-4, IL-13, and TNF- α mRNA expression. Data are mean ± standard deviation (SD, n = 6). ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effects of Cymbidium Root Ethanol Extract on Atopic Dermatitis

    doi: 10.1155/2016/5362475

    Figure Lengend Snippet: Effect of Cymbidium ethanol extract (CYM) on 2,4-dinitrochlorobenzene- (DNCB-) induced expression of interleukin- (IL-) 4, IL-13, and tumor necrosis factor- (TNF-) α mRNA in atopic dermatitis- (AD-) like mouse model. After inducing AD, 10 mg/mL CYM solution (in 3 : 1 mixture of acetone and olive oil) was applied to the dorsal skin of mice for a total of 6 times over a 2-week period. After euthanasia, dorsal skin lesions were enucleated from mice of all three groups (non-, DNCB, DNCB, and CYM treatment). Total RNA was isolated from extracted tissue of dorsal lesions using Tri-reagent. Total RNA was used as a template for cDNA synthesis, which was performed using a cDNA Synthesis kit. Real-time polymerase chain reaction (qPCR) analysis was carried out using SYBR Green I and a Lightcycler 96 instrument. The qPCR analysis was performed to detect IL-4, IL-13, and TNF- α mRNA expression. Data are mean ± standard deviation (SD, n = 6). ∗ P

    Article Snippet: The real-time polymerase chain reaction (qPCR) analysis was carried out using SYBR Green I and a Lightcycler® 96 instrument (Roche, Basel, Switzerland).

    Techniques: Expressing, Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, SYBR Green Assay, Standard Deviation

    Microbial distribution in a saponite-bearing locus near basaltic groundmass revealed by staining of a thin section with SYBR-Green I. (A) Back-scattered electron image of saponite aggregates enlarged from Figure 2B . An orange rectangle indicates the area shown in (B) . Fluorescence microscopy images of SYBR Green I-stained microbial cells associated with saponite in a 100-μm thin section of a rock piece (B) and in a 3-μm thin section of a clay fraction (C) .

    Journal: Frontiers in Microbiology

    Article Title: Deep Microbial Colonization in Saponite-Bearing Fractures in Aged Basaltic Crust: Implications for Subsurface Life on Mars

    doi: 10.3389/fmicb.2019.02793

    Figure Lengend Snippet: Microbial distribution in a saponite-bearing locus near basaltic groundmass revealed by staining of a thin section with SYBR-Green I. (A) Back-scattered electron image of saponite aggregates enlarged from Figure 2B . An orange rectangle indicates the area shown in (B) . Fluorescence microscopy images of SYBR Green I-stained microbial cells associated with saponite in a 100-μm thin section of a rock piece (B) and in a 3-μm thin section of a clay fraction (C) .

    Article Snippet: To demonstrate that microbial cells could be visually distinguishable from saponite, 3-μm thick thin sections of the clay fraction mainly composed of saponite were stained with SYBR-Green I and observed using the fluorescent microscope.

    Techniques: Staining, SYBR Green Assay, Fluorescence, Microscopy

    Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all SYBR Green I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.

    Journal: BMC Microbiology

    Article Title: Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry

    doi: 10.1186/1471-2180-14-56

    Figure Lengend Snippet: Growth and viability in pure and mixed cultures. Species-specific cell concentrations and viability were determined over a cultivation period of 32 h by qT-RFLP and flow cytometry, respectively. Open symbols represent data from pure cultures and filled symbols from mixed culture. Left: Dynamics of log 10 -transformed species-specific cell concentrations of (A) P. aeruginosa , (C) B. cepacia , and (E) S. aureus . Right: Dynamics of relative frequencies of (B) viable and dead cells of P. aeruginosa , (D) viable and dead cells of B. cepacia and (F) viable, damaged and dead cells of S. aureus . Relative frequencies of viability subpopulations were determined based on all SYBR Green I and PI fluorescence positive events in the respective species gate. Subpopulations were defined as gated in plots shown in Figure 2 . Error bars represent standard deviation of two (*) or three biological replicates.

    Article Snippet: SYBR Green I and PI for viability staining For staining, working solutions of 1:100 SYBR®Green I (10,000 × concentrate in DMSO, Life Technologies, Carlsbad, CA, USA) and 1 mg/mL propidium iodide (Sigma-Aldrich, Steinheim, Germany) were prepared in ultrapure water.

    Techniques: Flow Cytometry, Cytometry, Transformation Assay, SYBR Green Assay, Fluorescence, Standard Deviation

    Species discrimination in flow cytometric viability analysis. Four-color staining was applied. Samples were incubated with 20 μg/mL WGA-CF405S, 10 μg/mL 1°Ab and 60 μg/mL R-PE conjugated 2°Ab, SYBR Green I (dilution of 5 × 10 3 ) and 5 μg/mL PI. (A) For data analysis, only events with SYBR Green I positive fluorescence (cells) were considered. The cell gate was set manually based on the signal of four-color-stained (black) and unstained cells (red) in exponential growth phase (t = 4 h) from pure culture. (B, C) Gating for species discrimination in data acquired from mixed culture samples. Gate was set manually based on WGA-CF405S fluorescence and R-PE immunofluorescence signals of four-color-stained single species from pure culture. (B) Overlaid cytometric plot of P. aeruginosa (blue), B. cepacia (red) and S. aureus (green) in exponential growth phase (t = 4 h) from pure culture. (C) Gate applied for species discrimination in mixed culture sample at time point of inoculation. For each gate region, either relative frequencies of total events or cells (in italics) are shown. Data is presented in 5% quantile contour plots.

    Journal: BMC Microbiology

    Article Title: Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry

    doi: 10.1186/1471-2180-14-56

    Figure Lengend Snippet: Species discrimination in flow cytometric viability analysis. Four-color staining was applied. Samples were incubated with 20 μg/mL WGA-CF405S, 10 μg/mL 1°Ab and 60 μg/mL R-PE conjugated 2°Ab, SYBR Green I (dilution of 5 × 10 3 ) and 5 μg/mL PI. (A) For data analysis, only events with SYBR Green I positive fluorescence (cells) were considered. The cell gate was set manually based on the signal of four-color-stained (black) and unstained cells (red) in exponential growth phase (t = 4 h) from pure culture. (B, C) Gating for species discrimination in data acquired from mixed culture samples. Gate was set manually based on WGA-CF405S fluorescence and R-PE immunofluorescence signals of four-color-stained single species from pure culture. (B) Overlaid cytometric plot of P. aeruginosa (blue), B. cepacia (red) and S. aureus (green) in exponential growth phase (t = 4 h) from pure culture. (C) Gate applied for species discrimination in mixed culture sample at time point of inoculation. For each gate region, either relative frequencies of total events or cells (in italics) are shown. Data is presented in 5% quantile contour plots.

    Article Snippet: SYBR Green I and PI for viability staining For staining, working solutions of 1:100 SYBR®Green I (10,000 × concentrate in DMSO, Life Technologies, Carlsbad, CA, USA) and 1 mg/mL propidium iodide (Sigma-Aldrich, Steinheim, Germany) were prepared in ultrapure water.

    Techniques: Flow Cytometry, Staining, Incubation, Whole Genome Amplification, SYBR Green Assay, Fluorescence, Immunofluorescence

    Viability analysis by flow cytometry. Viability was determined by membrane integrity analysis using SYBR Green I (dilution of 5 × 10 3 ) and PI (5 μg/mL). Representative pseudo color dot plots with defined gates are shown for (A) P. aeruginosa , (B) B. cepacia and (C) S. aureus in stationary growth phase (t = 12 h) from pure culture. Gates were set manually for each species based on SYBR Green I and PI fluorescence signals of isopropanol-treated (red) and untreated cells (green). For each gate region, relative frequencies of total cells are presented. Only events with positive SYBR Green I fluorescence were considered as cells (see Figure 7 ).

    Journal: BMC Microbiology

    Article Title: Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry

    doi: 10.1186/1471-2180-14-56

    Figure Lengend Snippet: Viability analysis by flow cytometry. Viability was determined by membrane integrity analysis using SYBR Green I (dilution of 5 × 10 3 ) and PI (5 μg/mL). Representative pseudo color dot plots with defined gates are shown for (A) P. aeruginosa , (B) B. cepacia and (C) S. aureus in stationary growth phase (t = 12 h) from pure culture. Gates were set manually for each species based on SYBR Green I and PI fluorescence signals of isopropanol-treated (red) and untreated cells (green). For each gate region, relative frequencies of total cells are presented. Only events with positive SYBR Green I fluorescence were considered as cells (see Figure 7 ).

    Article Snippet: SYBR Green I and PI for viability staining For staining, working solutions of 1:100 SYBR®Green I (10,000 × concentrate in DMSO, Life Technologies, Carlsbad, CA, USA) and 1 mg/mL propidium iodide (Sigma-Aldrich, Steinheim, Germany) were prepared in ultrapure water.

    Techniques: Flow Cytometry, Cytometry, SYBR Green Assay, Fluorescence

    Comparison of NaCl-P buffer and Ringer solution for viability analysis of B. cepacia and S. aureus by flow cytometry. Viability was determined by membrane integrity analysis using SYBR Green I (dilution of 5 x 10 3 ) and PI (5 μg/mL). Relative frequencies of (A) viable and dead cells of B. cepacia and (B) of viable, damaged and dead cells of S. aureus in pure culture from both exponential and stationary growth phase. Relative frequencies were determined based on all SYBR Green I and PI fluorescence positive events. Error bars represent standard deviation of three biological replicates.

    Journal: BMC Microbiology

    Article Title: Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry

    doi: 10.1186/1471-2180-14-56

    Figure Lengend Snippet: Comparison of NaCl-P buffer and Ringer solution for viability analysis of B. cepacia and S. aureus by flow cytometry. Viability was determined by membrane integrity analysis using SYBR Green I (dilution of 5 x 10 3 ) and PI (5 μg/mL). Relative frequencies of (A) viable and dead cells of B. cepacia and (B) of viable, damaged and dead cells of S. aureus in pure culture from both exponential and stationary growth phase. Relative frequencies were determined based on all SYBR Green I and PI fluorescence positive events. Error bars represent standard deviation of three biological replicates.

    Article Snippet: SYBR Green I and PI for viability staining For staining, working solutions of 1:100 SYBR®Green I (10,000 × concentrate in DMSO, Life Technologies, Carlsbad, CA, USA) and 1 mg/mL propidium iodide (Sigma-Aldrich, Steinheim, Germany) were prepared in ultrapure water.

    Techniques: Flow Cytometry, Cytometry, SYBR Green Assay, Fluorescence, Standard Deviation

    Impact of KCL on flow cytometric viability determination. Comparison of Ringer solution without and with 3 M KCl. Viability was determined by membrane integrity analysis using SYBR Green I (dilution of 5 × 10 3 ) and PI (5 μg/mL). Relative frequencies of (A) viable and dead cells of P. aeruginosa, (B) of viable and dead cells of B. cepacia and (C) of viable, damaged and dead cells of S. aureus in exponential and stationary growth phases in pure culture. Relative frequencies were determined based on all SYBR Green I and PI fluorescence positive events. Error bars represent standard deviation of three biological replicates; # indicates a statistically significant difference (p

    Journal: BMC Microbiology

    Article Title: Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry

    doi: 10.1186/1471-2180-14-56

    Figure Lengend Snippet: Impact of KCL on flow cytometric viability determination. Comparison of Ringer solution without and with 3 M KCl. Viability was determined by membrane integrity analysis using SYBR Green I (dilution of 5 × 10 3 ) and PI (5 μg/mL). Relative frequencies of (A) viable and dead cells of P. aeruginosa, (B) of viable and dead cells of B. cepacia and (C) of viable, damaged and dead cells of S. aureus in exponential and stationary growth phases in pure culture. Relative frequencies were determined based on all SYBR Green I and PI fluorescence positive events. Error bars represent standard deviation of three biological replicates; # indicates a statistically significant difference (p

    Article Snippet: SYBR Green I and PI for viability staining For staining, working solutions of 1:100 SYBR®Green I (10,000 × concentrate in DMSO, Life Technologies, Carlsbad, CA, USA) and 1 mg/mL propidium iodide (Sigma-Aldrich, Steinheim, Germany) were prepared in ultrapure water.

    Techniques: Flow Cytometry, SYBR Green Assay, Fluorescence, Standard Deviation

    Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.

    Journal: International Journal of Analytical Chemistry

    Article Title: The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products

    doi: 10.1155/2016/4352308

    Figure Lengend Snippet: Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3–17 : 15 tobacco samples of different cured tobacco varieties; lanes 18-19 : two fresh tobacco samples; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.

    Article Snippet: The amplified LAMP products were examined either through visual inspection with 1000x SYBR Green I (Generay Biotech Co., Ltd., Shanghai, China) or on agarose gel electrophoresis (AGE) analysis.

    Techniques: SYBR Green Assay, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Marker

    Practical sample detection with different practical samples. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3-4 : coriander and spinach; lanes 5-6 : burley and Oriental SAADI-6; lanes 7-8 : NT . Oriental and NT . Virginia gold; lanes 9-10 : reconstituted tobacco (no tobacco stems, no sulphate cellulose) and reconstituted tobacco (tobacco stems, with sulphate cellulose); lanes 11-12 : cigarette (Liqun and Double Happiness); lanes 13-14 : wrappers from Liqun and Marlboro, respectively; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.

    Journal: International Journal of Analytical Chemistry

    Article Title: The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products

    doi: 10.1155/2016/4352308

    Figure Lengend Snippet: Practical sample detection with different practical samples. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : NTC; lane 2 : PTC; lanes 3-4 : coriander and spinach; lanes 5-6 : burley and Oriental SAADI-6; lanes 7-8 : NT . Oriental and NT . Virginia gold; lanes 9-10 : reconstituted tobacco (no tobacco stems, no sulphate cellulose) and reconstituted tobacco (tobacco stems, with sulphate cellulose); lanes 11-12 : cigarette (Liqun and Double Happiness); lanes 13-14 : wrappers from Liqun and Marlboro, respectively; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.

    Article Snippet: The amplified LAMP products were examined either through visual inspection with 1000x SYBR Green I (Generay Biotech Co., Ltd., Shanghai, China) or on agarose gel electrophoresis (AGE) analysis.

    Techniques: SYBR Green Assay, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Marker

    Specificity test of UMPS gene in tobacco and nontobacco plants. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : negative control (NTC); lane 2 : positive control (PTC); lanes 3–18 : Chinese jasmine, alfalfa, Altingia , canola, Pittosporum , Daphniphyllum , mondo grass, sapodilla, garden petunia, castor oil, indica rice, coriander, spinach, pomegranate, watermelon, and eggplant; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.

    Journal: International Journal of Analytical Chemistry

    Article Title: The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products

    doi: 10.1155/2016/4352308

    Figure Lengend Snippet: Specificity test of UMPS gene in tobacco and nontobacco plants. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1 : negative control (NTC); lane 2 : positive control (PTC); lanes 3–18 : Chinese jasmine, alfalfa, Altingia , canola, Pittosporum , Daphniphyllum , mondo grass, sapodilla, garden petunia, castor oil, indica rice, coriander, spinach, pomegranate, watermelon, and eggplant; lane M : Trans 2K DNA marker. Ct was expressed as mean Ct ± SD from 3 independent experiments with three replications.

    Article Snippet: The amplified LAMP products were examined either through visual inspection with 1000x SYBR Green I (Generay Biotech Co., Ltd., Shanghai, China) or on agarose gel electrophoresis (AGE) analysis.

    Techniques: SYBR Green Assay, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Negative Control, Positive Control, Marker

    Sensitivity test of UMPS gene using serial dilutions of genomic DNA from fresh tobaccoleave samples. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis. Lane 1 : NTC; lanes 2–9 : 120, 60, 30, 15, 7.5, 3.75, 1.88, and 0.94 ng per reaction, respectively; lane M : Trans 2K DNA marker. (c) qPCR method. For standard curve, a serial dilution of DNA samples (120, 60, 30, 15, 7.5, 3.75, 1.88, 0.94, 0.47, 0.24, 0.12, and 0.06 ng) was used. The result was developed after considering 3 independent experiments with three replications.

    Journal: International Journal of Analytical Chemistry

    Article Title: The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products

    doi: 10.1155/2016/4352308

    Figure Lengend Snippet: Sensitivity test of UMPS gene using serial dilutions of genomic DNA from fresh tobaccoleave samples. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis. Lane 1 : NTC; lanes 2–9 : 120, 60, 30, 15, 7.5, 3.75, 1.88, and 0.94 ng per reaction, respectively; lane M : Trans 2K DNA marker. (c) qPCR method. For standard curve, a serial dilution of DNA samples (120, 60, 30, 15, 7.5, 3.75, 1.88, 0.94, 0.47, 0.24, 0.12, and 0.06 ng) was used. The result was developed after considering 3 independent experiments with three replications.

    Article Snippet: The amplified LAMP products were examined either through visual inspection with 1000x SYBR Green I (Generay Biotech Co., Ltd., Shanghai, China) or on agarose gel electrophoresis (AGE) analysis.

    Techniques: SYBR Green Assay, Agarose Gel Electrophoresis, Marker, Real-time Polymerase Chain Reaction, Serial Dilution