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  • 99
    Millipore sybr green
    Sybr Green, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green/product/Millipore
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    sybr green - by Bioz Stars, 2020-08
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    99
    Bio-Rad iq sybr greeen supermix
    Reduction of CaMKIIδB transcripts and protein after Dox treatment in isolated cardiomyocytes. A , expression levels of CaMKIIδB and Dox-sensitive mRNAs in primary neonatal rat cardiomyocytes were analyzed by quantitative <t>RT-PCR.</t> B , rapid and severe reductions of CaMKIIδB transcripts in cardiomyocytes treated with Dox are shown. Expression of the indicated mRNAs was measured after the indicated time points of Dox exposure by real time PCR (IQ <t>SYBR</t> green, Bio-Rad). The data are corrected for GAPDH expression level and normalized so that expression at 0 h equals 1. Results shown are representative of three independent experiments. C ). The results are representative of three separate experiments. D , specificity of the band migrating at 55 kDa was demonstrated by transfecting primary cardiomyocytes with siCtr or siCaMKIIδB, and Western blot analysis was performed 72 h later using the CaMKIIδ-specific antibody. Untr , untreated; cTnI , cardiac troponin I.
    Iq Sybr Greeen Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iq sybr greeen supermix/product/Bio-Rad
    Average 99 stars, based on 2732 article reviews
    Price from $9.99 to $1999.99
    iq sybr greeen supermix - by Bioz Stars, 2020-08
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    92
    Bio-Rad sybr green
    Reduction of CaMKIIδB transcripts and protein after Dox treatment in isolated cardiomyocytes. A , expression levels of CaMKIIδB and Dox-sensitive mRNAs in primary neonatal rat cardiomyocytes were analyzed by quantitative <t>RT-PCR.</t> B , rapid and severe reductions of CaMKIIδB transcripts in cardiomyocytes treated with Dox are shown. Expression of the indicated mRNAs was measured after the indicated time points of Dox exposure by real time PCR (IQ <t>SYBR</t> green, Bio-Rad). The data are corrected for GAPDH expression level and normalized so that expression at 0 h equals 1. Results shown are representative of three independent experiments. C ). The results are representative of three separate experiments. D , specificity of the band migrating at 55 kDa was demonstrated by transfecting primary cardiomyocytes with siCtr or siCaMKIIδB, and Western blot analysis was performed 72 h later using the CaMKIIδ-specific antibody. Untr , untreated; cTnI , cardiac troponin I.
    Sybr Green, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 14783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green/product/Bio-Rad
    Average 92 stars, based on 14783 article reviews
    Price from $9.99 to $1999.99
    sybr green - by Bioz Stars, 2020-08
    92/100 stars
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    99
    Millipore kicqstart sybr green
    Reduction of CaMKIIδB transcripts and protein after Dox treatment in isolated cardiomyocytes. A , expression levels of CaMKIIδB and Dox-sensitive mRNAs in primary neonatal rat cardiomyocytes were analyzed by quantitative <t>RT-PCR.</t> B , rapid and severe reductions of CaMKIIδB transcripts in cardiomyocytes treated with Dox are shown. Expression of the indicated mRNAs was measured after the indicated time points of Dox exposure by real time PCR (IQ <t>SYBR</t> green, Bio-Rad). The data are corrected for GAPDH expression level and normalized so that expression at 0 h equals 1. Results shown are representative of three independent experiments. C ). The results are representative of three separate experiments. D , specificity of the band migrating at 55 kDa was demonstrated by transfecting primary cardiomyocytes with siCtr or siCaMKIIδB, and Western blot analysis was performed 72 h later using the CaMKIIδ-specific antibody. Untr , untreated; cTnI , cardiac troponin I.
    Kicqstart Sybr Green, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kicqstart sybr green/product/Millipore
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    kicqstart sybr green - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Reduction of CaMKIIδB transcripts and protein after Dox treatment in isolated cardiomyocytes. A , expression levels of CaMKIIδB and Dox-sensitive mRNAs in primary neonatal rat cardiomyocytes were analyzed by quantitative RT-PCR. B , rapid and severe reductions of CaMKIIδB transcripts in cardiomyocytes treated with Dox are shown. Expression of the indicated mRNAs was measured after the indicated time points of Dox exposure by real time PCR (IQ SYBR green, Bio-Rad). The data are corrected for GAPDH expression level and normalized so that expression at 0 h equals 1. Results shown are representative of three independent experiments. C ). The results are representative of three separate experiments. D , specificity of the band migrating at 55 kDa was demonstrated by transfecting primary cardiomyocytes with siCtr or siCaMKIIδB, and Western blot analysis was performed 72 h later using the CaMKIIδ-specific antibody. Untr , untreated; cTnI , cardiac troponin I.

    Journal: The Journal of Biological Chemistry

    Article Title: Critical Role of Nuclear Calcium/Calmodulin-dependent Protein Kinase II?B in Cardiomyocyte Survival in Cardiomyopathy *

    doi: 10.1074/jbc.M109.003186

    Figure Lengend Snippet: Reduction of CaMKIIδB transcripts and protein after Dox treatment in isolated cardiomyocytes. A , expression levels of CaMKIIδB and Dox-sensitive mRNAs in primary neonatal rat cardiomyocytes were analyzed by quantitative RT-PCR. B , rapid and severe reductions of CaMKIIδB transcripts in cardiomyocytes treated with Dox are shown. Expression of the indicated mRNAs was measured after the indicated time points of Dox exposure by real time PCR (IQ SYBR green, Bio-Rad). The data are corrected for GAPDH expression level and normalized so that expression at 0 h equals 1. Results shown are representative of three independent experiments. C ). The results are representative of three separate experiments. D , specificity of the band migrating at 55 kDa was demonstrated by transfecting primary cardiomyocytes with siCtr or siCaMKIIδB, and Western blot analysis was performed 72 h later using the CaMKIIδ-specific antibody. Untr , untreated; cTnI , cardiac troponin I.

    Article Snippet: Real time PCRs were performed after RT-PCR (RETROscriptTM , Ambion) with various dilutions of cDNA template using IQ SYBR Green and an Opticon 2 reader (Bio-Rad).

    Techniques: Isolation, Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, SYBR Green Assay, Western Blot

    (A and B). 293T cells were transfected with our experimental and control plasmids and 24 hours later the cells were washed and treated with IFNβ at 1000 U/ml for an additional 12 h. Total cellular RNA was then extracted and cDNA was generated by reverse transcription. Real-time PCRs were carried out using SYBR green supermix. MxA hand ISG15 relative gene expression was calculated using the comparative threshold cycle method, employing 18S rRNA and GAPDH as references. (C and D). 293T cells were transfected with our experimental and control plasmids and 24 hours later the cells were washed and treated with IFN-γ (5 ng/ml) for 12 hours. Total cellular RNA was then extracted and cDNA was generated by reverse transcription. Real-time PCRs were carried out using SYBR green supermix. IP10 and IRF1 relative gene expression was calculated using the comparative threshold cycle method, employing 18S rRNA and GAPDH as references

    Journal: Virus research

    Article Title: La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation

    doi: 10.1016/j.virusres.2015.10.027

    Figure Lengend Snippet: (A and B). 293T cells were transfected with our experimental and control plasmids and 24 hours later the cells were washed and treated with IFNβ at 1000 U/ml for an additional 12 h. Total cellular RNA was then extracted and cDNA was generated by reverse transcription. Real-time PCRs were carried out using SYBR green supermix. MxA hand ISG15 relative gene expression was calculated using the comparative threshold cycle method, employing 18S rRNA and GAPDH as references. (C and D). 293T cells were transfected with our experimental and control plasmids and 24 hours later the cells were washed and treated with IFN-γ (5 ng/ml) for 12 hours. Total cellular RNA was then extracted and cDNA was generated by reverse transcription. Real-time PCRs were carried out using SYBR green supermix. IP10 and IRF1 relative gene expression was calculated using the comparative threshold cycle method, employing 18S rRNA and GAPDH as references

    Article Snippet: Real-time PCRs were carried out using SYBR green supermix (BioRad).

    Techniques: Transfection, Generated, SYBR Green Assay, Expressing