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  • 99
    ATCC svg a cells
    Importance of the actin network during JUNV entry. (A, B) <t>Vero,</t> SUM159, <t>SVG-A,</t> or A549 cells were untreated or pretreated for 15 min with 1 μM latrunculin A (Lat A), 1 μM latrunculin B (Lat B), 1 μM cytochalasin D (Cyto D), or
    Svg A Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen svg a cells
    Expansions are suppressed by siRNA knockdown of <t>proteasome</t> components in <t>SVG-A</t> cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits PSMC5 and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P
    Svg A Cells, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher svg a cells
    Expansions are suppressed by siRNA knockdown of <t>proteasome</t> components in <t>SVG-A</t> cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits PSMC5 and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P
    Svg A Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Corning Life Sciences svg a cells
    VP1 pentamers of JCPyV Mad-1 with PML-associated mutations exhibit reduced binding to cells. (A) <t>SVG-A</t> cells were incubated with 100 µg/ml of His-tagged wild-type (WT) or mutant pentamers in PBS, washed, and then incubated with a penta-His Alexa Fluor 488 antibody. Pentamer binding was analyzed by flow cytometry. Histograms represent the fluorescence intensity of Alexa 488 for antibody alone (gray) and pentamer samples for 10,000 gated events. (B) Quantitation of binding of VP1 pentamers with PML-associated mutations. Bar graph represents the mean fluorescence intensity of VP1 pentamers binding to SVG-A cells for 3 independent experiments. Error bars indicate standard deviations.
    Svg A Cells, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 30 article reviews
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    85
    Iwaki America indirect immunofluorescence analysis svg a cells
    C80A and C247A mutations decrease infectivity. (A) Expression of JCV-encoded agnoprotein and Vp1 after JCV genome transfection. <t>SVG-A</t> cells were transfected with WT JCV genome for 3 days, fixed, and examined for the presence of agnoprotein and Vp1 by immunofluorescence analysis. Cell nuclei were counterstained with DAPI. Merged images of DAPI (Blue), Agno (Red), and Vp1 (Green) are also presented. (B) Levels of Vp1 expression. SVG-A cells were transfected with JCV genomes encoding either WT Vp1 or cysteine point mutant Vp1s or transfected with the transfection reagent alone (vehicle) for 3 days, and cell lysates were analyzed by SDS-PAGE and immunoblotting for Vp1 (Vp1) and for actin (act). (C) Subcellular localization of C80A and C247A Vp1s. SVG-A cells at 3 days posttransfection with the WT or either mutant JCV genome were processed for immunofluorescence analysis with a mouse anti-Vp1 antibody ( green ). Cell nuclei were counterstained with DAPI ( blue ). Merged images of DAPI and Vp1 are also presented. (D) Infectivity of WT and mutant JCV genomes. JCV genomes encoding either WT or individual cysteine mutant Vp1s were transfected into SVG-A cells, and the effectiveness of infection initiation was determined by immunofluorescence analysis for the presence of agnoprotein. For each genome, the average percentage of cells positive for agnoprotein is presented in the bar graph as the mean ± SD of three microscopic fields. The data represent the mean ± SD of three independent experiments. The significance of the changes was analyzed by Student's t -test (* p
    Indirect Immunofluorescence Analysis Svg A Cells, supplied by Iwaki America, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Importance of the actin network during JUNV entry. (A, B) Vero, SUM159, SVG-A, or A549 cells were untreated or pretreated for 15 min with 1 μM latrunculin A (Lat A), 1 μM latrunculin B (Lat B), 1 μM cytochalasin D (Cyto D), or

    Journal: Journal of Virology

    Article Title: Identification and Characterization of a Novel Broad-Spectrum Virus Entry Inhibitor

    doi: 10.1128/JVI.00103-16

    Figure Lengend Snippet: Importance of the actin network during JUNV entry. (A, B) Vero, SUM159, SVG-A, or A549 cells were untreated or pretreated for 15 min with 1 μM latrunculin A (Lat A), 1 μM latrunculin B (Lat B), 1 μM cytochalasin D (Cyto D), or

    Article Snippet: Vero and SVG-A cells very efficiently supported JUNV infection and spread ( and ), produced infectious viral particles ( and ), and exhibited no major cell death due to JUNV infection over time ( ).

    Techniques:

    Characterization of infection of Vero, SUM159, SVG-A, and A549 cells by JUNV. (A, B) JUNV (MOI, 0.1) was inoculated onto Vero, SUM159, SVG-A, or A549 cells for 1 h at 37°C, and the cells were subsequently washed and reincubated for the indicated

    Journal: Journal of Virology

    Article Title: Identification and Characterization of a Novel Broad-Spectrum Virus Entry Inhibitor

    doi: 10.1128/JVI.00103-16

    Figure Lengend Snippet: Characterization of infection of Vero, SUM159, SVG-A, and A549 cells by JUNV. (A, B) JUNV (MOI, 0.1) was inoculated onto Vero, SUM159, SVG-A, or A549 cells for 1 h at 37°C, and the cells were subsequently washed and reincubated for the indicated

    Article Snippet: Vero and SVG-A cells very efficiently supported JUNV infection and spread ( and ), produced infectious viral particles ( and ), and exhibited no major cell death due to JUNV infection over time ( ).

    Techniques: Infection

    Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits PSMC5 and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P

    Journal: Nucleic Acids Research

    Article Title: The 26S proteasome drives trinucleotide repeat expansions

    doi: 10.1093/nar/gkt295

    Figure Lengend Snippet: Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits PSMC5 and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P

    Article Snippet: Proteasome activity assay Approximately 5 × 106 SVG-A cells treated with siRNA, as outlined in Supplementary Figure S5 , were resuspended in lysis buffer (13 mM Tris–Cl and 5 mM MgCl2 , pH 7.8) and subjected to two rounds of freeze–thaw lysis.

    Techniques:

    VP1 pentamers of JCPyV Mad-1 with PML-associated mutations exhibit reduced binding to cells. (A) SVG-A cells were incubated with 100 µg/ml of His-tagged wild-type (WT) or mutant pentamers in PBS, washed, and then incubated with a penta-His Alexa Fluor 488 antibody. Pentamer binding was analyzed by flow cytometry. Histograms represent the fluorescence intensity of Alexa 488 for antibody alone (gray) and pentamer samples for 10,000 gated events. (B) Quantitation of binding of VP1 pentamers with PML-associated mutations. Bar graph represents the mean fluorescence intensity of VP1 pentamers binding to SVG-A cells for 3 independent experiments. Error bars indicate standard deviations.

    Journal: mBio

    Article Title: Progressive Multifocal Leukoencephalopathy-Associated Mutations in the JC Polyomavirus Capsid Disrupt Lactoseries Tetrasaccharide c Binding

    doi: 10.1128/mBio.00247-13

    Figure Lengend Snippet: VP1 pentamers of JCPyV Mad-1 with PML-associated mutations exhibit reduced binding to cells. (A) SVG-A cells were incubated with 100 µg/ml of His-tagged wild-type (WT) or mutant pentamers in PBS, washed, and then incubated with a penta-His Alexa Fluor 488 antibody. Pentamer binding was analyzed by flow cytometry. Histograms represent the fluorescence intensity of Alexa 488 for antibody alone (gray) and pentamer samples for 10,000 gated events. (B) Quantitation of binding of VP1 pentamers with PML-associated mutations. Bar graph represents the mean fluorescence intensity of VP1 pentamers binding to SVG-A cells for 3 independent experiments. Error bars indicate standard deviations.

    Article Snippet: SVG-A cells at 70% confluence in 24-well plates (Corning) were infected with 150 µl of virus supernatant at 37°C for 1 h; then 1 ml of medium containing 5% FBS, 1% P/S, and 1% amphotericin B was added to cells; and cells were incubated at 37°C for 72 h. Cells were fixed and stained by indirect immunofluorescence.

    Techniques: Binding Assay, Incubation, Mutagenesis, Flow Cytometry, Cytometry, Fluorescence, Quantitation Assay

    JCPyV with PML-associated mutations are not infectious. (A) PML-associated mutations are highlighted on the wild-type Mad-1 JCPyV VP1 pentamer (surface representation) in complex with LSTc (stick model) ( 41 ). Residues in pink indicate mutations used in this study, while those labeled in black represent other PML-associated mutations. (B) Growth of JCPyV VP1 wild-type (WT) and PML-associated mutant viruses. SVG-A cells were transfected with linearized DNA from wild-type and mutant JCPyV. Transfected cells were fixed and stained at day 4 posttransfection and then at 3-day intervals for 22 days by indirect immunofluorescence. Transfected or infected cells were quantified based on nuclear VP1 staining. Each data point represents the average number of infected cells per visual field for 6 fields of view for 3 independent experiments. Error bars indicate standard deviations. (C) Infectivity of supernatants from JCPyV VP1 wild-type and mutant viruses. SVG-A cells were inoculated with supernatants harvested from infected cells at day 22 from panel B. Cells were fixed and stained by indirect immunofluorescence at 72 h postinfection and quantified based on nuclear VP1 staining. The results are presented as the average number of infected cells per visual field for 6 visual fields from 3 individual samples performed in triplicate. Error bars indicate standard deviations.

    Journal: mBio

    Article Title: Progressive Multifocal Leukoencephalopathy-Associated Mutations in the JC Polyomavirus Capsid Disrupt Lactoseries Tetrasaccharide c Binding

    doi: 10.1128/mBio.00247-13

    Figure Lengend Snippet: JCPyV with PML-associated mutations are not infectious. (A) PML-associated mutations are highlighted on the wild-type Mad-1 JCPyV VP1 pentamer (surface representation) in complex with LSTc (stick model) ( 41 ). Residues in pink indicate mutations used in this study, while those labeled in black represent other PML-associated mutations. (B) Growth of JCPyV VP1 wild-type (WT) and PML-associated mutant viruses. SVG-A cells were transfected with linearized DNA from wild-type and mutant JCPyV. Transfected cells were fixed and stained at day 4 posttransfection and then at 3-day intervals for 22 days by indirect immunofluorescence. Transfected or infected cells were quantified based on nuclear VP1 staining. Each data point represents the average number of infected cells per visual field for 6 fields of view for 3 independent experiments. Error bars indicate standard deviations. (C) Infectivity of supernatants from JCPyV VP1 wild-type and mutant viruses. SVG-A cells were inoculated with supernatants harvested from infected cells at day 22 from panel B. Cells were fixed and stained by indirect immunofluorescence at 72 h postinfection and quantified based on nuclear VP1 staining. The results are presented as the average number of infected cells per visual field for 6 visual fields from 3 individual samples performed in triplicate. Error bars indicate standard deviations.

    Article Snippet: SVG-A cells at 70% confluence in 24-well plates (Corning) were infected with 150 µl of virus supernatant at 37°C for 1 h; then 1 ml of medium containing 5% FBS, 1% P/S, and 1% amphotericin B was added to cells; and cells were incubated at 37°C for 72 h. Cells were fixed and stained by indirect immunofluorescence.

    Techniques: Labeling, Mutagenesis, Transfection, Staining, Immunofluorescence, Infection

    C80A and C247A mutations decrease infectivity. (A) Expression of JCV-encoded agnoprotein and Vp1 after JCV genome transfection. SVG-A cells were transfected with WT JCV genome for 3 days, fixed, and examined for the presence of agnoprotein and Vp1 by immunofluorescence analysis. Cell nuclei were counterstained with DAPI. Merged images of DAPI (Blue), Agno (Red), and Vp1 (Green) are also presented. (B) Levels of Vp1 expression. SVG-A cells were transfected with JCV genomes encoding either WT Vp1 or cysteine point mutant Vp1s or transfected with the transfection reagent alone (vehicle) for 3 days, and cell lysates were analyzed by SDS-PAGE and immunoblotting for Vp1 (Vp1) and for actin (act). (C) Subcellular localization of C80A and C247A Vp1s. SVG-A cells at 3 days posttransfection with the WT or either mutant JCV genome were processed for immunofluorescence analysis with a mouse anti-Vp1 antibody ( green ). Cell nuclei were counterstained with DAPI ( blue ). Merged images of DAPI and Vp1 are also presented. (D) Infectivity of WT and mutant JCV genomes. JCV genomes encoding either WT or individual cysteine mutant Vp1s were transfected into SVG-A cells, and the effectiveness of infection initiation was determined by immunofluorescence analysis for the presence of agnoprotein. For each genome, the average percentage of cells positive for agnoprotein is presented in the bar graph as the mean ± SD of three microscopic fields. The data represent the mean ± SD of three independent experiments. The significance of the changes was analyzed by Student's t -test (* p

    Journal: PLoS ONE

    Article Title: Cysteine Residues in the Major Capsid Protein, Vp1, of the JC Virus Are Important for Protein Stability and Oligomer Formation

    doi: 10.1371/journal.pone.0076668

    Figure Lengend Snippet: C80A and C247A mutations decrease infectivity. (A) Expression of JCV-encoded agnoprotein and Vp1 after JCV genome transfection. SVG-A cells were transfected with WT JCV genome for 3 days, fixed, and examined for the presence of agnoprotein and Vp1 by immunofluorescence analysis. Cell nuclei were counterstained with DAPI. Merged images of DAPI (Blue), Agno (Red), and Vp1 (Green) are also presented. (B) Levels of Vp1 expression. SVG-A cells were transfected with JCV genomes encoding either WT Vp1 or cysteine point mutant Vp1s or transfected with the transfection reagent alone (vehicle) for 3 days, and cell lysates were analyzed by SDS-PAGE and immunoblotting for Vp1 (Vp1) and for actin (act). (C) Subcellular localization of C80A and C247A Vp1s. SVG-A cells at 3 days posttransfection with the WT or either mutant JCV genome were processed for immunofluorescence analysis with a mouse anti-Vp1 antibody ( green ). Cell nuclei were counterstained with DAPI ( blue ). Merged images of DAPI and Vp1 are also presented. (D) Infectivity of WT and mutant JCV genomes. JCV genomes encoding either WT or individual cysteine mutant Vp1s were transfected into SVG-A cells, and the effectiveness of infection initiation was determined by immunofluorescence analysis for the presence of agnoprotein. For each genome, the average percentage of cells positive for agnoprotein is presented in the bar graph as the mean ± SD of three microscopic fields. The data represent the mean ± SD of three independent experiments. The significance of the changes was analyzed by Student's t -test (* p

    Article Snippet: Indirect Immunofluorescence Analysis SVG-A cells seeded onto glass-bottomed dishes (Iwaki, Tokyo, Japan) were transfected with WT or mutant JCV genomes.

    Techniques: Infection, Expressing, Transfection, Immunofluorescence, Mutagenesis, SDS Page, Activated Clotting Time Assay