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  • 99
    ATCC cell culture saos 2 cells
    Cell Culture Saos 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture saos 2 cells/product/ATCC
    Average 99 stars, based on 8 article reviews
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    cell culture saos 2 cells - by Bioz Stars, 2020-08
    99/100 stars
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    96
    Thermo Fisher high fidelity pcr
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    High Fidelity Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    high fidelity pcr - by Bioz Stars, 2020-08
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    91
    TaKaRa pmd18 t cloning vector
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    Pmd18 T Cloning Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 499 article reviews
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    pmd18 t cloning vector - by Bioz Stars, 2020-08
    91/100 stars
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    99
    Bio-Rad icycler detection system
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    Icycler Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icycler detection system/product/Bio-Rad
    Average 99 stars, based on 756 article reviews
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    icycler detection system - by Bioz Stars, 2020-08
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    89
    Biotechnology Information phylogenetic sequence analysis
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    Phylogenetic Sequence Analysis, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phylogenetic sequence analysis - by Bioz Stars, 2020-08
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    92
    Bio-Rad real time qrt pcr
    Analysis of tomato RNAi LIN5 . A, Screening of total invertase activity in primary Lin5i transformants in extracts of tissue sampled from young fruit. B and C, <t>qRT-PCR</t> of LIN5 and LIN7 , respectively, in young fruit of wild-type (WT) and Lin5i transgenic
    Real Time Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Biotechnology Information genbank
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Genbank, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 94/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genbank/product/Biotechnology Information
    Average 94 stars, based on 6786 article reviews
    Price from $9.99 to $1999.99
    genbank - by Bioz Stars, 2020-08
    94/100 stars
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    85
    Thermo Fisher fluorescent alexa conjugate
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Fluorescent Alexa Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent alexa conjugate/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    fluorescent alexa conjugate - by Bioz Stars, 2020-08
    85/100 stars
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    93
    Biotechnology Information ncbi national center
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    94
    Biotechnology Information open reading frame
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Open Reading Frame, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    open reading frame - by Bioz Stars, 2020-08
    94/100 stars
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    93
    Biotechnology Information exons
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    Waters Corporation mass lynx software
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Mass Lynx Software, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega point mutant rnase h minus
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Point Mutant Rnase H Minus, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescript rt reagent kit
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    Intel xeon e5 2680 v4
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    TaKaRa pcr buffer ii
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Pcr Buffer Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad dc protein assay kit
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    Abmart anti susi 1 antibody
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Anti Susi 1 Antibody, supplied by Abmart, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Oligo Dt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt/product/Thermo Fisher
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    oligo dt - by Bioz Stars, 2020-08
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    Servicebio Inc anti actin
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Anti Actin, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biogazelle qbaseplus version 2 5 software
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    Polysciences inc polybead polystyrene microsphere
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
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    Cells expressing α2 integrin attach on EV1 coated surface through interaction between nonactivated integrins and the virus, and activate FAK signaling. ( a ) Integrin α2 transfected SaOS cells (SaOS α2+ ) and α2 integrin negative wild type SaOS cells (SaOS α2− ) spreading on EV1 or BSA coated surface. Photographed through a phase contrast microscope after 0, 20, 40, and 60 min. Scale bar 50 μm. ( b ) Confocal microscopy image of SaOS α2+ cell on EV1-coated surface. α2 integrins were labelled using specific antibodies. Scale bar 50 μm. ( c ) Western blotting detection of phosphorylation of FAK at Y397 in SaOS α2+ cells on collagen I or an immobilized layer of EV1. Typical experiment on the left, and on the right, a visualization of the results from ten independent experiments presented in box blot as natural logarithm of pY397FAK band intensity normalized to β-actin. Whiskers present minimum and maximum of all of the data. Non-parametric Mann-Whitney U test was used to determine statistical significance. ( d ) Western blotting analysis of FAK Y397 phosphorylation SaOS α2+ cells plated on collagen I or an immobilized layer of EV1, time points 0 to 120 min. ( e ) Western blotting of FAK Y397 phosphorylation in <t>HeLa</t> <t>OHIO</t> cells plated on collagen I or an immobilized layer of EV1. ( f ) Western blotting of FAK Y397 phosphorylation in SaOS α2+ cells spreading on Collagen I or poly-L-lysine.
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    Cells expressing α2 integrin attach on EV1 coated surface through interaction between nonactivated integrins and the virus, and activate FAK signaling. ( a ) Integrin α2 transfected SaOS cells (SaOS α2+ ) and α2 integrin negative wild type SaOS cells (SaOS α2− ) spreading on EV1 or BSA coated surface. Photographed through a phase contrast microscope after 0, 20, 40, and 60 min. Scale bar 50 μm. ( b ) Confocal microscopy image of SaOS α2+ cell on EV1-coated surface. α2 integrins were labelled using specific antibodies. Scale bar 50 μm. ( c ) Western blotting detection of phosphorylation of FAK at Y397 in SaOS α2+ cells on collagen I or an immobilized layer of EV1. Typical experiment on the left, and on the right, a visualization of the results from ten independent experiments presented in box blot as natural logarithm of pY397FAK band intensity normalized to β-actin. Whiskers present minimum and maximum of all of the data. Non-parametric Mann-Whitney U test was used to determine statistical significance. ( d ) Western blotting analysis of FAK Y397 phosphorylation SaOS α2+ cells plated on collagen I or an immobilized layer of EV1, time points 0 to 120 min. ( e ) Western blotting of FAK Y397 phosphorylation in <t>HeLa</t> <t>OHIO</t> cells plated on collagen I or an immobilized layer of EV1. ( f ) Western blotting of FAK Y397 phosphorylation in SaOS α2+ cells spreading on Collagen I or poly-L-lysine.
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    Image Search Results


    Validation of PsnSuSy2 integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using PCR amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p

    Journal: PLoS ONE

    Article Title: Overexpression of Poplar Xylem Sucrose Synthase in Tobacco Leads to a Thickened Cell Wall and Increased Height

    doi: 10.1371/journal.pone.0120669

    Figure Lengend Snippet: Validation of PsnSuSy2 integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using PCR amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p

    Article Snippet: The full-length coding region of PsnSuSy2 was obtained by high-fidelity PCR (Thermo Scientific) with two specific primers, SuSy2 -F ( 5`-ATGTCTGTACTTACTCGTGTCC-3` ) and SuSy2 -R ( 5`-TTACTCGATAGTCAAAGGAACAG-3` ) and cloned the PCR products into the pMD18-T cloning vector (TaKaRa Biotechnology, Dalian, China).

    Techniques: Expressing, Transgenic Assay, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Marker, Quantitative RT-PCR, Activity Assay

    Tissue-specific expression of the PsnSuSy2 in one-year-old hybrid Populus simonii × Populus nigra propagated from two years old hybrid trees. a. Tissue-specific expression pattern characterized by RT-PCR. b. Tissue-specific expression pattern characterized by real-time quantitative PCR. The expression levels for real-time quantitative PCR were averaged from three replicates.

    Journal: PLoS ONE

    Article Title: Overexpression of Poplar Xylem Sucrose Synthase in Tobacco Leads to a Thickened Cell Wall and Increased Height

    doi: 10.1371/journal.pone.0120669

    Figure Lengend Snippet: Tissue-specific expression of the PsnSuSy2 in one-year-old hybrid Populus simonii × Populus nigra propagated from two years old hybrid trees. a. Tissue-specific expression pattern characterized by RT-PCR. b. Tissue-specific expression pattern characterized by real-time quantitative PCR. The expression levels for real-time quantitative PCR were averaged from three replicates.

    Article Snippet: The full-length coding region of PsnSuSy2 was obtained by high-fidelity PCR (Thermo Scientific) with two specific primers, SuSy2 -F ( 5`-ATGTCTGTACTTACTCGTGTCC-3` ) and SuSy2 -R ( 5`-TTACTCGATAGTCAAAGGAACAG-3` ) and cloned the PCR products into the pMD18-T cloning vector (TaKaRa Biotechnology, Dalian, China).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Analysis of tomato RNAi LIN5 . A, Screening of total invertase activity in primary Lin5i transformants in extracts of tissue sampled from young fruit. B and C, qRT-PCR of LIN5 and LIN7 , respectively, in young fruit of wild-type (WT) and Lin5i transgenic

    Journal: Plant Physiology

    Article Title: RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility 1RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility 1 [W]RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility 1 [W] [OA]

    doi: 10.1104/pp.109.136598

    Figure Lengend Snippet: Analysis of tomato RNAi LIN5 . A, Screening of total invertase activity in primary Lin5i transformants in extracts of tissue sampled from young fruit. B and C, qRT-PCR of LIN5 and LIN7 , respectively, in young fruit of wild-type (WT) and Lin5i transgenic

    Article Snippet: Expression of the LIN5 , LIN7 , SuSy2 , SuSy3 , SGN-U331270 , and hormone-related genes was analyzed by real-time qRT-PCR using the fluorescent intercalating dye SYBR Green in an iCycler detection system (Bio-Rad; ) as described by .

    Techniques: Activity Assay, Quantitative RT-PCR, Transgenic Assay

    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from Genbank ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.

    Journal: PLoS ONE

    Article Title: Overexpression of Poplar Xylem Sucrose Synthase in Tobacco Leads to a Thickened Cell Wall and Increased Height

    doi: 10.1371/journal.pone.0120669

    Figure Lengend Snippet: Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from Genbank ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.

    Article Snippet: Phylogenetic sequence analysis A total of 116 putative plant SuSy2 protein sequences from both dicotyledon and monocotyledon plants were retrieved from GenBank at the National Center for Biotechnology Information ( http://www.ncbi.nlm.nih.gov/genbank ) and aligned with PsnSuSy2 using ClustalW within the MEGA 4.0 software package [ ].

    Techniques: Derivative Assay, Generated

    Cells expressing α2 integrin attach on EV1 coated surface through interaction between nonactivated integrins and the virus, and activate FAK signaling. ( a ) Integrin α2 transfected SaOS cells (SaOS α2+ ) and α2 integrin negative wild type SaOS cells (SaOS α2− ) spreading on EV1 or BSA coated surface. Photographed through a phase contrast microscope after 0, 20, 40, and 60 min. Scale bar 50 μm. ( b ) Confocal microscopy image of SaOS α2+ cell on EV1-coated surface. α2 integrins were labelled using specific antibodies. Scale bar 50 μm. ( c ) Western blotting detection of phosphorylation of FAK at Y397 in SaOS α2+ cells on collagen I or an immobilized layer of EV1. Typical experiment on the left, and on the right, a visualization of the results from ten independent experiments presented in box blot as natural logarithm of pY397FAK band intensity normalized to β-actin. Whiskers present minimum and maximum of all of the data. Non-parametric Mann-Whitney U test was used to determine statistical significance. ( d ) Western blotting analysis of FAK Y397 phosphorylation SaOS α2+ cells plated on collagen I or an immobilized layer of EV1, time points 0 to 120 min. ( e ) Western blotting of FAK Y397 phosphorylation in HeLa OHIO cells plated on collagen I or an immobilized layer of EV1. ( f ) Western blotting of FAK Y397 phosphorylation in SaOS α2+ cells spreading on Collagen I or poly-L-lysine.

    Journal: Scientific Reports

    Article Title: Integrin α2β1 in nonactivated conformation can induce focal adhesion kinase signaling

    doi: 10.1038/s41598-017-03640-w

    Figure Lengend Snippet: Cells expressing α2 integrin attach on EV1 coated surface through interaction between nonactivated integrins and the virus, and activate FAK signaling. ( a ) Integrin α2 transfected SaOS cells (SaOS α2+ ) and α2 integrin negative wild type SaOS cells (SaOS α2− ) spreading on EV1 or BSA coated surface. Photographed through a phase contrast microscope after 0, 20, 40, and 60 min. Scale bar 50 μm. ( b ) Confocal microscopy image of SaOS α2+ cell on EV1-coated surface. α2 integrins were labelled using specific antibodies. Scale bar 50 μm. ( c ) Western blotting detection of phosphorylation of FAK at Y397 in SaOS α2+ cells on collagen I or an immobilized layer of EV1. Typical experiment on the left, and on the right, a visualization of the results from ten independent experiments presented in box blot as natural logarithm of pY397FAK band intensity normalized to β-actin. Whiskers present minimum and maximum of all of the data. Non-parametric Mann-Whitney U test was used to determine statistical significance. ( d ) Western blotting analysis of FAK Y397 phosphorylation SaOS α2+ cells plated on collagen I or an immobilized layer of EV1, time points 0 to 120 min. ( e ) Western blotting of FAK Y397 phosphorylation in HeLa OHIO cells plated on collagen I or an immobilized layer of EV1. ( f ) Western blotting of FAK Y397 phosphorylation in SaOS α2+ cells spreading on Collagen I or poly-L-lysine.

    Article Snippet: HeLa OHIO cells, originally from ATCC, were a gift from Dr. Petri Susi from department of Virology, University of Turku, Finland.

    Techniques: Expressing, Transfection, Microscopy, Confocal Microscopy, Western Blot, MANN-WHITNEY