superscript kit Bio Rad Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Bio-Rad superscript cdna kit bio rad
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Cdna Kit Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript cdna kit bio rad/product/Bio-Rad
    Average 92 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    superscript cdna kit bio rad - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    90
    Bio-Rad superscript rt kit
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Rt Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript rt kit/product/Bio-Rad
    Average 90 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    superscript rt kit - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    86
    Bio-Rad superscript mixture kit
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Mixture Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript mixture kit/product/Bio-Rad
    Average 86 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    superscript mixture kit - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    88
    Bio-Rad superscript synthesis kit
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript synthesis kit/product/Bio-Rad
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    superscript synthesis kit - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    Bio-Rad superscript reverse transcriptase kit
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Reverse Transcriptase Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript reverse transcriptase kit/product/Bio-Rad
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    superscript reverse transcriptase kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Bio-Rad superscript vilo cdna synthesis kit
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Vilo Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript vilo cdna synthesis kit/product/Bio-Rad
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    superscript vilo cdna synthesis kit - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Bio-Rad superscript iii reverse transcriptase
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Iii Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcriptase/product/Bio-Rad
    Average 91 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcriptase - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    89
    Bio-Rad superscript iii cellsdirect cdna synthesis kit
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript Iii Cellsdirect Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii cellsdirect cdna synthesis kit/product/Bio-Rad
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    superscript iii cellsdirect cdna synthesis kit - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    90
    Bio-Rad superscript one step sybr green qrt pcr kit
    <t>SREBP</t> expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid <t>cDNA.</t> The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.
    Superscript One Step Sybr Green Qrt Pcr Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript one step sybr green qrt pcr kit/product/Bio-Rad
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    superscript one step sybr green qrt pcr kit - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    SREBP expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid cDNA. The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.

    Journal: Cell metabolism

    Article Title: Linking Lipid Metabolism to the Innate Immune Response in Macrophages through Sterol Regulatory Element Binding Protein -1a

    doi: 10.1016/j.cmet.2011.04.001

    Figure Lengend Snippet: SREBP expression levels in BMDM of WT and SREBP-1aDF mice Total RNA was isolated from BMDM and equal amounts of RNA were used for the quantitative RT-PCR assay. RNA expression level for SREBP-1a (A), SREBP-1c (B), and SREBP-2 (C) are shown as the quantitative expression levels calculated by comparison to qPCR values obtained with a standard curve of serially diluted linear plasmid cDNA. The data are plotted as femtograms of RNA normalized to ribosomal L32 samples analyzed in parallel. Bars represent the standard deviations. (D) nuclear SREBP-1 protein level. Nuclear protein was prepared from BMDM of WT and SREBP-1aDF mice. Aliquots (30 µg) were analyzed by immunoblotting for SREBP-1. *, p = 0.0031, indicating a statistically significant difference between BMDM from the two lines of mice.

    Article Snippet: Total RNA was isolated from macrophages of WT and SREBP-1aDF mice using the Trizol method (Invitrogen). cDNA was synthesized by cDNA superscript kit (Bio-Rad) and used for qPCR with Real-Time PCR Detection (Bio-Rad). mRNA levels were normalized for expression of ribosomal protein L32 mRNA as control and calculated by the comparative threshold cycle method.

    Techniques: Expressing, Mouse Assay, Isolation, Quantitative RT-PCR, RNA Expression, Real-time Polymerase Chain Reaction, Plasmid Preparation