Journal: The Journal of Investigative Dermatology
Article Title: Increased Bacterial Load and Expression of Antimicrobial Peptides in Skin of Barrier-Deficient Mice with Reduced Cancer Susceptibility
Figure Lengend Snippet: AMP expression and protease activity in EPI-/- epidermis after microbiota modulation. ( a–k ) qRT-PCR of Camp ( a ) , Defb1 ( b ), Defb2 ( c ), Defb3 ( d ), Defb6 ( e ), S100A9 ( f ), Pla2g2a ( g ), Reg3b ( h ), Reg3g ( i ), and Serpina1b ( j ) in WT, EPI-/-, antibiotic-treated EPI-/-, and flora-deficient EPI-/- epidermis. Data are means ± SEM from at least four mice per group. * P
Article Snippet: RNA concentration was measured using an ND-100 NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE). cDNA was synthesized using a Superscript III First-Strand Synthesis Supermix for quantitative reverse transcriptase PCR (qRT-PCR) kit (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. qRT-PCR was carried out using specific primers and fast SYBR Green and run on an ABI Prism 7900HT Sequence detection system.
Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Mouse Assay